##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553024_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	355902
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.259799045804744	31.0	31.0	34.0	30.0	34.0
2	31.437468741395104	31.0	31.0	34.0	30.0	34.0
3	31.60901034554456	31.0	31.0	34.0	30.0	34.0
4	35.39646307129491	37.0	35.0	37.0	33.0	37.0
5	34.76902630499407	35.0	35.0	37.0	32.0	37.0
6	34.786865485442625	35.0	35.0	37.0	32.0	37.0
7	34.616321346887624	35.0	35.0	37.0	31.0	37.0
8	35.11087040814606	36.0	35.0	37.0	32.0	37.0
9	36.76582036628061	39.0	35.0	39.0	32.0	39.0
10	36.13357047726621	37.0	35.0	39.0	32.0	39.0
11	36.13670898168597	37.0	35.0	39.0	32.0	39.0
12	35.54738102061803	37.0	35.0	39.0	30.0	39.0
13	35.459907502627125	37.0	35.0	39.0	30.0	39.0
14	36.19181122893381	38.0	35.0	40.0	30.0	41.0
15	36.39227371579817	38.0	35.0	40.0	31.0	41.0
16	36.247396755286566	38.0	34.0	40.0	30.0	41.0
17	36.09518350557176	38.0	34.0	40.0	30.0	41.0
18	35.77840529134425	37.0	34.0	40.0	29.0	41.0
19	35.73198801917382	37.0	34.0	40.0	29.0	41.0
20	35.752802737832326	37.0	34.0	40.0	30.0	41.0
21	35.94950295306011	37.0	34.0	40.0	30.0	41.0
22	35.92191108788374	37.0	34.0	40.0	30.0	41.0
23	35.721339020292106	37.0	34.0	40.0	30.0	41.0
24	35.212519176627275	37.0	33.0	40.0	27.0	41.0
25	35.394861506819296	37.0	34.0	40.0	28.0	41.0
26	35.329601969081374	37.0	34.0	40.0	27.0	41.0
27	35.26264814471399	37.0	34.0	40.0	27.0	41.0
28	35.150853324791655	37.0	33.0	40.0	27.0	41.0
29	34.90745486116965	37.0	33.0	40.0	26.0	41.0
30	34.99741220897888	37.0	34.0	40.0	26.0	41.0
31	34.682480008541674	37.0	33.0	40.0	25.0	41.0
32	34.362192401279	37.0	33.0	40.0	24.0	41.0
33	34.48108468061432	37.0	33.0	40.0	24.0	41.0
34	34.370956049699075	37.0	33.0	40.0	24.0	41.0
35	34.20898730549421	37.0	33.0	40.0	23.0	41.0
36	34.0645542874162	37.0	33.0	40.0	23.0	41.0
37	33.69892835668246	37.0	33.0	40.0	21.0	41.0
38	33.61553461346101	36.0	33.0	40.0	21.0	41.0
39	33.238298745160186	36.0	32.0	39.0	19.0	41.0
40	32.95714831610949	36.0	31.0	39.0	18.0	40.0
41	33.166846491449895	36.0	32.0	39.0	18.0	40.0
42	32.969092615382884	36.0	32.0	39.0	17.0	40.0
43	32.98563930520199	36.0	32.0	39.0	17.0	40.0
44	32.865974341251246	36.0	32.0	39.0	16.0	40.0
45	32.41441183247073	35.0	31.0	39.0	15.0	40.0
46	32.4132092542329	35.0	31.0	39.0	15.0	40.0
47	32.294384409191295	35.0	31.0	39.0	15.0	40.0
48	32.333709841473215	35.0	31.0	39.0	13.0	40.0
49	32.156661103337434	35.0	31.0	39.0	12.0	40.0
50	31.920053835044477	35.0	30.0	39.0	11.0	40.0
51	30.050435232170653	34.0	26.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	5.0
11	6.0
12	2.0
13	11.0
14	12.0
15	48.0
16	104.0
17	253.0
18	494.0
19	1053.0
20	1838.0
21	2877.0
22	3967.0
23	4784.0
24	5279.0
25	5547.0
26	5830.0
27	6536.0
28	7510.0
29	9123.0
30	11424.0
31	14620.0
32	18777.0
33	24302.0
34	35669.0
35	39560.0
36	36774.0
37	44625.0
38	48815.0
39	26052.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.04683311698164	47.10650684739057	16.89594326528089	19.950716770346894
2	15.859983928159998	48.6816595579682	16.369112845671	19.089243668200798
3	16.192378800905868	32.16334833746368	31.958235694095567	19.686037167534884
4	26.884648021084455	32.912711926316796	16.22581497153711	23.97682508106164
5	13.508774887469022	46.933987445982325	18.156121628987755	21.401116037560904
6	19.60033941927834	35.00879455580469	27.75707919595844	17.633786828958534
7	61.80830678108018	22.785766868407595	9.523408129204107	5.882518221308112
8	64.30646638681435	11.699568982472703	5.8704362436850595	18.12352838702789
9	64.39469292108501	14.698709195227899	12.204764232850616	8.701833650836466
10	34.71686025928486	35.30859618659069	14.382891919685756	15.591651634438694
11	27.06025816095442	26.49212423644711	27.778714365190417	18.66890323740805
12	25.127141741265852	26.60591960708285	34.62189029564318	13.645048356008116
13	22.30895021663267	37.339492332158855	20.6863125242342	19.665244926974278
14	12.111199150327899	35.980129361453436	36.948654404864264	14.960017083354407
15	11.421121544694888	35.01553798517569	28.6477738253789	24.915566644750523
16	14.334564009193542	31.054054205933095	34.94304611943737	19.66833566543599
17	13.822625329444621	32.749464740293675	27.154947148372305	26.272962781889397
18	16.216823732375765	33.75816938370675	24.9650184601379	25.05998842377958
19	17.07801585829807	36.831768295766814	30.191738175115624	15.898477670819494
20	18.627599732510635	41.012132553343335	22.024040325707638	18.336227388438388
21	14.116807435754787	33.54828014453417	27.00687267843395	25.32803974127709
22	15.468303072194031	38.554433523835215	26.1926035818849	19.784659822085853
23	18.678175452793187	33.76519378930155	19.064517760507105	28.492112997398163
24	12.635781760147458	44.540912947946346	23.422178015296346	19.401127276609852
25	15.452849379885475	31.837696894088825	26.258632994476006	26.450820731549697
26	18.769492725525566	38.8269804609134	19.379492107377875	23.024034706183162
27	12.099679125152429	34.04504610819832	28.708464689717957	25.146810076931292
28	20.06029749762575	29.595787604452912	23.54440267264584	26.799512225275496
29	14.180308062331765	35.65138717961686	31.31114745070272	18.85715730734865
30	20.149647936791588	29.995897747132638	20.430905136807322	29.42354917926845
31	26.81131322667476	33.511191282993636	21.179425796989058	18.49806969334255
32	20.173811892037698	27.91920247708639	24.21818365729892	27.688801973577
33	23.793909559373088	27.423279442093612	24.770582913273877	24.012228085259427
34	16.209518350557175	26.53679945603003	31.94587274024872	25.307809453164072
35	19.925990862653205	26.4994296182657	25.18333698602424	28.391242533056854
36	24.337317576186702	23.522205550966277	33.90680580609269	18.233671066754333
37	19.738298745160186	27.42580822810774	26.363156149726606	26.47273687700547
38	27.277452781945595	26.49184326022332	28.676433400205674	17.554270557625415
39	18.026872566043462	25.73095964619474	36.98433838528584	19.257829402475963
40	25.76776753151148	27.32521873999022	27.033565419694185	19.873448308804107
41	18.471095975858525	21.702322549465862	38.410011744806155	21.416569729869458
42	27.783490960994882	24.42975875381425	23.336761243263595	24.44998904192727
43	22.14289326837163	23.54131193418413	30.009665582098442	24.3061292153458
44	19.95717922349411	23.351372006900775	27.726733763788907	28.964715005816206
45	27.31988019173818	29.728408382082712	23.009423942545983	19.942287483633134
46	17.17607656040146	23.899275643295066	39.08266882456407	19.84197897173941
47	22.373855724328607	27.138931503616163	25.15945400700193	25.3277587650533
48	19.29800900247821	21.227753707481273	37.24620822585993	22.22802906418059
49	23.251063495007052	20.53458536338655	30.42017184505847	25.794179296547924
50	20.569145438912958	23.430326325786314	25.346022219599778	30.65450601570095
51	16.133092817685768	24.031053492253484	35.29567128029626	24.540182409764487
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5948.0
1	4799.5
2	3651.0
3	2615.5
4	1580.0
5	1579.0
6	1578.0
7	1816.0
8	2054.0
9	2410.5
10	2767.0
11	3074.0
12	3381.0
13	3548.5
14	3716.0
15	3564.0
16	3412.0
17	3151.5
18	2891.0
19	2744.0
20	2597.0
21	2405.5
22	2214.0
23	2226.5
24	2239.0
25	2344.5
26	2601.0
27	2752.0
28	2849.5
29	2947.0
30	3289.5
31	3632.0
32	4060.0
33	4488.0
34	5182.0
35	5876.0
36	6464.0
37	7052.0
38	7678.0
39	8304.0
40	10359.5
41	12415.0
42	16008.0
43	19601.0
44	38407.5
45	57214.0
46	54343.0
47	51472.0
48	45055.0
49	38638.0
50	33495.5
51	28353.0
52	24817.0
53	21281.0
54	18159.0
55	15037.0
56	12809.5
57	10582.0
58	9262.5
59	7943.0
60	6808.0
61	5673.0
62	4632.5
63	3592.0
64	3066.0
65	2540.0
66	2224.0
67	1908.0
68	1486.0
69	1064.0
70	1012.5
71	961.0
72	877.5
73	794.0
74	711.0
75	445.0
76	262.0
77	198.0
78	134.0
79	141.5
80	149.0
81	105.5
82	62.0
83	43.0
84	24.0
85	20.5
86	17.0
87	14.0
88	11.0
89	9.5
90	8.0
91	5.0
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	2.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	355902.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.54184438240667
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.49456066874195	30.219142702561037
2	8.731089999593115	6.555624441070237
3	3.0977793036122487	3.4888904564175345
4	1.61852555516044	2.430497380831264
5	1.017517215415959	1.9099736478782852
6	0.6814265537860471	1.534920578416527
7	0.4885154947499695	1.283784087760843
8	0.38505823670445616	1.1564637120418086
9	0.3281082370151228	1.108600953714679
>10	2.767142568976354	21.607169110316672
>50	0.32585438064545397	8.131545728914672
>100	0.05093815605492154	3.380293261682668
>500	0.005243633711536041	1.414267455324637
>1k	0.005243633711536041	4.052696022804404
>5k	7.490905302194344E-4	1.4153923449291903
>10k+	0.0022472715906583034	10.310738115335552
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGT	13587	3.8176239526611253	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCC	12411	3.487195913481801	No Hit
AATCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCT	10666	2.996892402964861	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5033	1.4141533343448478	No Hit
CTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGCT	3854	1.0828823664941474	TruSeq Adapter, Index 27 (95% over 23bp)
AATGACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTT	2980	0.8373091468999894	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCT	2002	0.5625144000314694	No Hit
AACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTG	1733	0.48693179583143675	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTAT	1644	0.46192491191395385	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1180	0.33155194407449246	No Hit
AAACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCT	1018	0.2860337958201977	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	947	0.2660844839309698	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTT	860	0.24163955246107072	No Hit
AATGCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTC	739	0.20764142938224567	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCGTTTGTC	666	0.18713016504543384	No Hit
AGGGAGGGACATACACACACGCACACACACCCACACACAGTAATCTGTAAC	629	0.17673404476513196	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	621	0.17448623497479643	No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCCTCGTTTGTCGTATGCCGT	567	0.1593135188900315	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATG	493	0.13852127832942776	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCT	475	0.1334637063011728	No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGC	413	0.11604318042607235	No Hit
AATCTGTCTCTTATACCCATCTGACGCCTCGTTTGTCGTATGCCGTCTTCT	413	0.11604318042607235	No Hit
AATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTA	358	0.1005894881175155	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.3790313063708548	0.0
2	0.0	0.0	0.0	1.657759720372462	0.0
3	0.0	0.0	0.0	2.6667453400093284	0.0
4	0.0	0.0	0.0	7.955279824221274	0.0
5	0.0	0.0	0.0	8.510488842434153	0.0
6	0.0	0.0	0.0	10.944866845367544	0.0
7	0.0	0.0	0.0	12.68972919511551	0.0
8	0.0	0.0	0.0	13.545020820338182	0.0
9	0.0	0.0	0.0	19.097672954914554	0.0
10	0.0	0.0	0.0	20.830453327039464	0.0
11	0.0	0.0	0.0	26.53623750358245	0.0
12	0.0	0.0	0.0	27.596360795949447	0.0
13	0.0	0.0	0.0	28.170395221156387	0.0
14	0.0	0.0	0.0	29.087501615613288	0.0
15	0.0	0.0	0.0	29.52470061983355	0.0
16	0.0	0.0	0.0	30.144815145742367	0.0
17	0.0	0.0	0.0	30.88181578074863	0.0
18	0.0	0.0	0.0	32.03775196542868	0.0
19	0.0	0.0	0.0	32.49518125776197	0.0
20	0.0	0.0	0.0	32.92114121303055	0.0
21	0.0	0.0	0.0	33.431675011660516	0.0
22	0.0	0.0	0.0	33.94951419210906	0.0
23	0.0	0.0	0.0	34.40891031800889	0.0
24	0.0	0.0	0.0	34.77614624250496	0.0
25	0.0	0.0	0.0	35.03464436839354	0.0
26	0.0	0.0	0.0	35.32348792645166	0.0
27	0.0	0.0	0.0	35.68594725514327	0.0
28	0.0	0.0	0.0	35.98265814746756	0.0
29	0.0	0.0	0.0	36.346522357278126	0.0
30	0.0	0.0	0.0	36.80648043562554	0.0
31	0.0	0.0	0.0	37.129884069210064	0.0
32	0.0	0.0	0.0	37.4111412692258	0.0
33	0.0	0.0	0.0	37.7143146146973	0.0
34	0.0	0.0	0.0	37.99276205247512	0.0
35	0.0	0.0	0.0	38.32431399654961	0.0
36	2.809762237919427E-4	0.0	0.0	38.615686340621856	0.0
37	2.809762237919427E-4	0.0	0.0	38.92756994903091	0.0
38	2.809762237919427E-4	0.0	0.0	39.232148175621376	0.0
39	2.809762237919427E-4	0.0	0.0	39.71458435187215	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	30	2.1612632E-6	45.000004	21
GTGCAGA	30	2.1612632E-6	45.000004	19
TAATACG	30	2.1612632E-6	45.000004	1
AGCAACG	30	2.1612632E-6	45.000004	1
CTCACGT	30	2.1612632E-6	45.000004	45
CTCACGA	30	2.1612632E-6	45.000004	24
ACACGAC	30	2.1612632E-6	45.000004	26
CCTAGCG	30	2.1612632E-6	45.000004	1
GCAACGG	60	0.0	45.000004	2
CACGACC	30	2.1612632E-6	45.000004	27
CGAGACA	30	2.1612632E-6	45.000004	22
CCTATGT	30	2.1612632E-6	45.000004	10
TACAGCG	30	2.1612632E-6	45.000004	1
CGTCCGG	30	2.1612632E-6	45.000004	2
GCGTAGG	30	2.1612632E-6	45.000004	2
GCTCACG	30	2.1612632E-6	45.000004	44
AACACGT	30	2.1612632E-6	45.000004	41
TGATCGG	20	7.026717E-4	45.0	2
AGGTACG	20	7.026717E-4	45.0	1
AGCCGAC	20	7.026717E-4	45.0	41
>>END_MODULE