SRR3553023_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553023_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	313082
Sequences flagged as poor quality	0
Sequence length	46
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTAT	9971	3.18478864961895	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGT	9045	2.8890194901016346	No Hit
AATCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGT	7518	2.401287841523946	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5827	1.8611737500079852	No Hit
CTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTT	3344	1.0680907877169559	No Hit
AATGACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCC	2117	0.6761806810995202	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCGT	1912	0.6107026274266805	No Hit
AACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTC	1539	0.49156451025609904	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGC	1474	0.4708031761647109	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCGTTTGT	1452	0.4637762630876256	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1296	0.4139490612682939	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1055	0.33697242256022386	No Hit
AAACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGT	1000	0.31940513986751073	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCC	773	0.24690017311758578	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	682	0.21783430538964235	No Hit
AATGCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCG	602	0.19228189420024147	No Hit
AGGGAGGGACATACACACACGCACACACACCCACACACAGTAATCT	493	0.1574667339546828	No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCCTCGTTTGTCGTAT	457	0.1459681489194524	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGC	455	0.1453293386397174	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	425	0.13574718444369208	No Hit
CCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCT	385	0.12297097884899164	No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCCTCGTTTGTCG	382	0.12201276342938909	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCCTCGTTTGTC	366	0.11690228119150894	No Hit
AATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTCG	339	0.10827834241508615	No Hit
AATCTGTCTCTTATACCCATCTGACGCCTCGTTTGTCGTATGCCGT	326	0.1041260755968085	No Hit
AACCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGT	325	0.103806670456941	No Hit
AATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTCGTTTG	325	0.103806670456941	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAT	45	1.2150849E-9	40.0	18
TTGATGG	30	4.856158E-6	40.0	2
GTCATAG	20	0.0012543697	40.0	1
GTGCAGG	55	5.456968E-12	40.0	2
CGGGTGA	20	0.0012543697	40.0	6
AACGTAG	20	0.0012543697	40.0	13
CGGGTAC	35	3.04688E-7	40.0	6
CGGCGAT	100	0.0	40.0	30
CGGCGAA	20	0.0012543697	40.0	30
GGAGCTA	20	0.0012543697	40.0	8
ACAGTAG	30	4.856158E-6	40.0	1
TCGCACG	30	4.856158E-6	40.0	1
CGAACCC	20	0.0012543697	40.0	33
ACACGAG	25	7.781648E-5	40.0	37
CCCTCGA	45	1.2150849E-9	40.0	40
GTACAGT	20	0.0012543697	40.0	13
TACCAGG	45	1.2150849E-9	40.0	2
ACGCATT	20	0.0012543697	40.0	27
TCACACG	30	4.856158E-6	40.0	1
ACCGCTA	20	0.0012543697	40.0	36

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content