##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553023_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	313082
Sequences flagged as poor quality	0
Sequence length	46
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.09819152809807	31.0	31.0	33.0	28.0	34.0
2	31.33927533361867	31.0	31.0	34.0	30.0	34.0
3	31.568767926613475	31.0	31.0	34.0	30.0	34.0
4	35.46085051200644	37.0	35.0	37.0	33.0	37.0
5	34.809050025233006	35.0	35.0	37.0	32.0	37.0
6	34.92642822008292	35.0	35.0	37.0	32.0	37.0
7	34.215253511859515	35.0	35.0	37.0	30.0	37.0
8	35.11916047553037	35.0	35.0	37.0	32.0	37.0
9	36.50672347819421	38.0	35.0	39.0	32.0	39.0
10	36.19687813416294	37.0	35.0	39.0	32.0	39.0
11	36.31297870845338	37.0	35.0	39.0	32.0	39.0
12	35.816137625286665	37.0	35.0	39.0	31.0	39.0
13	35.500220389546506	37.0	35.0	39.0	30.0	39.0
14	36.44132208175494	38.0	35.0	40.0	31.0	41.0
15	36.59146166180106	38.0	35.0	40.0	31.0	41.0
16	36.41800550654461	38.0	35.0	40.0	31.0	41.0
17	36.41329108668017	38.0	35.0	40.0	31.0	41.0
18	36.14016455752806	38.0	35.0	40.0	30.0	41.0
19	35.82733916354182	37.0	34.0	40.0	29.0	41.0
20	35.851633118480144	37.0	34.0	40.0	30.0	41.0
21	36.0913754224133	38.0	34.0	40.0	30.0	41.0
22	35.952622635603454	38.0	34.0	40.0	30.0	41.0
23	35.710085536696454	37.0	34.0	40.0	29.0	41.0
24	35.31572239860484	37.0	34.0	40.0	27.0	41.0
25	35.61497946224951	37.0	34.0	40.0	29.0	41.0
26	35.44954357005513	37.0	34.0	40.0	28.0	41.0
27	35.282437188979245	37.0	34.0	40.0	27.0	41.0
28	35.41602519467743	37.0	34.0	40.0	27.0	41.0
29	35.01706581662312	37.0	33.0	40.0	25.0	41.0
30	35.311969388211395	37.0	34.0	40.0	27.0	41.0
31	34.926482518956696	37.0	34.0	40.0	25.0	41.0
32	34.616637174925415	37.0	33.0	40.0	24.0	41.0
33	34.60839013421404	37.0	34.0	40.0	24.0	41.0
34	34.395864342249	37.0	34.0	40.0	23.0	41.0
35	34.2451817734651	37.0	33.0	40.0	22.0	41.0
36	34.17734012175724	37.0	33.0	40.0	22.0	41.0
37	33.97173583917312	37.0	33.0	40.0	21.0	41.0
38	33.772717690573074	37.0	33.0	40.0	20.0	41.0
39	33.51405702020557	37.0	33.0	40.0	19.0	41.0
40	33.25028906165158	36.0	32.0	40.0	18.0	41.0
41	33.365709302994105	37.0	33.0	40.0	17.0	41.0
42	33.32814406449429	37.0	33.0	40.0	16.0	41.0
43	33.42059588222894	37.0	33.0	40.0	17.0	41.0
44	33.37243916929111	37.0	33.0	40.0	17.0	41.0
45	33.10870315125111	36.0	32.0	40.0	15.0	41.0
46	31.975402610178804	35.0	30.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	9.0
12	7.0
13	2.0
14	12.0
15	27.0
16	49.0
17	105.0
18	221.0
19	452.0
20	993.0
21	1909.0
22	3044.0
23	4071.0
24	4780.0
25	5112.0
26	5263.0
27	5222.0
28	5745.0
29	6685.0
30	8324.0
31	10743.0
32	14449.0
33	19303.0
34	29808.0
35	34760.0
36	31607.0
37	41619.0
38	50222.0
39	28536.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.37813735698635	46.40605336621077	16.912182750844824	20.303626525958055
2	16.308187631355363	47.97401319782038	15.871241400016608	19.84655777080765
3	16.44712886719773	32.27237592707342	31.356641391073264	19.923853814655583
4	27.234079250803305	33.05843197628736	16.224503484710077	23.482985288199256
5	13.523613621990405	47.226285765390536	17.812905245271207	21.437195367347854
6	18.9327396656467	35.10549951769824	28.404060278137994	17.557700538517064
7	61.984080847829006	23.23768214078101	8.978478481675726	5.79975852971426
8	64.30136513756779	11.635290435093681	5.801355555413598	18.261988871924927
9	66.29062034866266	14.500673944845122	11.494752173551976	7.713953532940251
10	36.82932905756319	34.83144990769191	13.526488268249212	14.812732766495678
11	25.933780926402665	26.414166256763405	26.98302681086744	20.669026005966487
12	27.83136686235555	26.150976421512574	32.382890105467574	13.634766610664299
13	21.50778390325857	39.015018429676566	21.128969407375703	18.348228259689154
14	12.095233836502898	35.45908100753157	34.584549734574324	17.8611354213912
15	11.29672098683412	34.026868360365654	29.07097821018136	25.605432442618863
16	14.080336780779476	33.68095259388914	33.327690509195676	18.91102011613571
17	13.576634875208413	31.890686784931745	26.427900677777703	28.104777662082135
18	16.141138743204657	32.54035683942226	24.8235286602232	26.494975757149884
19	19.18123686446362	35.988015919152176	29.055327358327848	15.775419858056356
20	18.815517979315324	41.18888981161485	21.917900102848456	18.077692106221374
21	14.039772328016303	33.57075782063485	29.03392721395673	23.35554263739212
22	17.209548936061477	36.72232833570758	25.91940769510863	20.148715033122315
23	17.533745153027002	35.897304859429795	19.880414715633606	26.688535271909597
24	12.708811110188384	43.95589653828709	23.652589417468906	19.682702934055616
25	15.109779546572463	31.9235855143381	28.259689154917883	24.70694578417156
26	19.63798621447416	37.89710044014028	20.555956586453387	21.908956758932167
27	11.864303920378687	36.46840124951291	27.527612574341546	24.139682255766857
28	18.817753815294395	29.607259440018908	26.65340070652417	24.921586038162527
29	14.211612293265022	37.17428660862011	30.056343066672632	18.557758031442244
30	19.198165336876603	30.27737142346095	20.726199526002773	29.79826371365968
31	27.032215202407038	33.261254240103234	21.126094761116896	18.580435796372836
32	21.91374783603018	28.163867612957628	23.46445979008694	26.457924760925255
33	22.826607725771524	27.9271884043158	24.22847688465003	25.017726985262644
34	17.9623868507292	26.482838361834922	31.095367986661643	24.45940680077424
35	18.8196702461336	27.94251985102944	25.451479165202727	27.78633073763423
36	23.41750723452642	23.662490976804797	33.365699720839906	19.554302067828875
37	20.13913287892629	27.292849796538928	26.043336889377223	26.52468043515756
38	25.963485604410348	27.822742923579124	27.80325921004721	18.41051226196332
39	17.822167994327366	25.632901284647474	35.07355900371149	21.471371717313676
40	25.118020199181046	26.64701260372682	26.52595805571703	21.709009141375102
41	18.71714119623613	21.915344861729515	36.559431714375144	22.808082227659206
42	26.439399262812934	24.963428111485168	23.77013050894015	24.827042116761746
43	21.28324208993171	23.49128982183581	30.989964290505362	24.235503797727112
44	20.268811365712498	23.370235273826026	27.665914999904178	28.695038360557295
45	27.096415635520405	29.25783021700385	23.394190659316088	20.25156348815965
46	17.234462536971147	25.431037236251207	37.74761883468229	19.58688139209536
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6847.0
1	5466.0
2	4085.0
3	3076.5
4	2068.0
5	2231.0
6	2394.0
7	2394.0
8	2449.5
9	2505.0
10	2674.0
11	2843.0
12	3009.5
13	3176.0
14	3081.5
15	2987.0
16	2823.5
17	2660.0
18	2501.0
19	2342.0
20	2342.0
21	2183.5
22	2025.0
23	2081.5
24	2138.0
25	2184.0
26	2230.0
27	2455.0
28	2680.0
29	2897.0
30	3114.0
31	3114.0
32	3349.0
33	3584.0
34	4145.5
35	4707.0
36	5471.0
37	6235.0
38	7512.0
39	8789.0
40	15646.5
41	22504.0
42	22306.0
43	22108.0
44	22108.0
45	34463.0
46	46818.0
47	43327.0
48	39836.0
49	35961.5
50	32087.0
51	28292.5
52	24498.0
53	20669.0
54	16840.0
55	14901.5
56	12963.0
57	12963.0
58	11167.0
59	9371.0
60	7904.0
61	6437.0
62	5268.5
63	4100.0
64	3487.5
65	2875.0
66	2289.5
67	1704.0
68	1577.0
69	1450.0
70	1450.0
71	1053.0
72	656.0
73	665.5
74	675.0
75	535.0
76	395.0
77	295.5
78	196.0
79	147.0
80	98.0
81	98.0
82	64.5
83	31.0
84	26.0
85	21.0
86	13.0
87	5.0
88	3.5
89	2.0
90	1.0
91	0.0
92	1.5
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	313082.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.25523720783006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.95645672184344	24.926947485433516
2	10.90923514657597	7.2557840511076135
3	3.9608956063508782	3.95161568833951
4	2.102599821990506	2.7968982333374215
5	1.3964116753448945	2.3219000751688923
6	0.9252569284337341	1.8461783179951243
7	0.6867036629330678	1.5985545241627468
8	0.5529558718709511	1.4710942947624772
9	0.47011227259929594	1.407032562564149
>10	3.6521035870574328	25.26650735916563
>50	0.3124100635694292	6.765307247171775
>100	0.05888879792522786	3.9263477297024294
>500	0.003992460876286635	1.0146965094006533
>1k	0.00798492175257327	4.709692107257399
>5k	0.003992460876286635	10.741443814430664
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTAT	9971	3.18478864961895	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGT	9045	2.8890194901016346	No Hit
AATCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGT	7518	2.401287841523946	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5827	1.8611737500079852	No Hit
CTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTT	3344	1.0680907877169559	No Hit
AATGACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCC	2117	0.6761806810995202	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCGT	1912	0.6107026274266805	No Hit
AACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTC	1539	0.49156451025609904	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGC	1474	0.4708031761647109	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCGTTTGT	1452	0.4637762630876256	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1296	0.4139490612682939	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1055	0.33697242256022386	No Hit
AAACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGT	1000	0.31940513986751073	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCC	773	0.24690017311758578	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	682	0.21783430538964235	No Hit
AATGCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCG	602	0.19228189420024147	No Hit
AGGGAGGGACATACACACACGCACACACACCCACACACAGTAATCT	493	0.1574667339546828	No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCCTCGTTTGTCGTAT	457	0.1459681489194524	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGC	455	0.1453293386397174	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	425	0.13574718444369208	No Hit
CCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCT	385	0.12297097884899164	No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCCTCGTTTGTCG	382	0.12201276342938909	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCCTCGTTTGTC	366	0.11690228119150894	No Hit
AATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTCG	339	0.10827834241508615	No Hit
AATCTGTCTCTTATACCCATCTGACGCCTCGTTTGTCGTATGCCGT	326	0.1041260755968085	No Hit
AACCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGT	325	0.103806670456941	No Hit
AATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTCGTTTG	325	0.103806670456941	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.1940513986751073E-4	0.0	0.0	1.1635929245373418	0.0
2	3.1940513986751073E-4	0.0	0.0	1.486511520943395	0.0
3	3.1940513986751073E-4	0.0	0.0	2.388192230789378	0.0
4	3.1940513986751073E-4	0.0	0.0	6.864655266032541	0.0
5	3.1940513986751073E-4	0.0	0.0	7.401575306149827	0.0
6	3.1940513986751073E-4	0.0	0.0	9.71598494962981	0.0
7	3.1940513986751073E-4	0.0	0.0	11.35708855826908	0.0
8	3.1940513986751073E-4	0.0	0.0	12.258130457835328	0.0
9	3.1940513986751073E-4	0.0	0.0	16.899725950389993	0.0
10	3.1940513986751073E-4	0.0	0.0	18.771759475153473	0.0
11	3.1940513986751073E-4	0.0	0.0	23.733718322995255	0.0
12	3.1940513986751073E-4	0.0	0.0	24.86569013868571	0.0
13	3.1940513986751073E-4	0.0	0.0	25.43327307223028	0.0
14	3.1940513986751073E-4	0.0	0.0	26.3119566120058	0.0
15	3.1940513986751073E-4	0.0	0.0	26.742834145687073	0.0
16	3.1940513986751073E-4	0.0	0.0	27.395059441296528	0.0
17	3.1940513986751073E-4	0.0	0.0	28.17376917229352	0.0
18	6.388102797350215E-4	0.0	0.0	29.884822506563776	0.0
19	6.388102797350215E-4	0.0	0.0	30.449211388709667	0.0
20	6.388102797350215E-4	0.0	0.0	30.896697989664048	0.0
21	6.388102797350215E-4	0.0	0.0	31.46619735404782	0.0
22	6.388102797350215E-4	0.0	0.0	32.00279798902524	0.0
23	6.388102797350215E-4	0.0	0.0	32.48541915536505	0.0
24	6.388102797350215E-4	0.0	0.0	32.83133492184156	0.0
25	6.388102797350215E-4	0.0	0.0	33.10219048044921	0.0
26	6.388102797350215E-4	0.0	0.0	33.406583578742946	0.0
27	6.388102797350215E-4	0.0	0.0	33.778690566688596	0.0
28	6.388102797350215E-4	0.0	0.0	34.10192856823452	0.0
29	6.388102797350215E-4	0.0	0.0	34.45199660152931	0.0
30	6.388102797350215E-4	0.0	0.0	34.900760823043164	0.0
31	6.388102797350215E-4	0.0	0.0	35.22623466056816	0.0
32	9.582154196025322E-4	0.0	0.0	35.50315891683329	0.0
33	9.582154196025322E-4	0.0	0.0	35.81489833334398	0.0
34	9.582154196025322E-4	0.0	0.0	36.13941395544937	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAT	45	1.2150849E-9	40.0	18
TTGATGG	30	4.856158E-6	40.0	2
GTCATAG	20	0.0012543697	40.0	1
GTGCAGG	55	5.456968E-12	40.0	2
CGGGTGA	20	0.0012543697	40.0	6
AACGTAG	20	0.0012543697	40.0	13
CGGGTAC	35	3.04688E-7	40.0	6
CGGCGAT	100	0.0	40.0	30
CGGCGAA	20	0.0012543697	40.0	30
GGAGCTA	20	0.0012543697	40.0	8
ACAGTAG	30	4.856158E-6	40.0	1
TCGCACG	30	4.856158E-6	40.0	1
CGAACCC	20	0.0012543697	40.0	33
ACACGAG	25	7.781648E-5	40.0	37
CCCTCGA	45	1.2150849E-9	40.0	40
GTACAGT	20	0.0012543697	40.0	13
TACCAGG	45	1.2150849E-9	40.0	2
ACGCATT	20	0.0012543697	40.0	27
TCACACG	30	4.856158E-6	40.0	1
ACCGCTA	20	0.0012543697	40.0	36
>>END_MODULE