SRR3553022_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553022_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	350937
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4564	1.3005183266512224	No Hit
CTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGCT	3525	1.0044537908513496	No Hit
AATCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCT	2897	0.8255042927932934	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGT	2449	0.6978460521404127	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCC	1847	0.5263052912631042	No Hit
AAACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCT	1463	0.4168839421320636	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1112	0.31686599019197176	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTT	957	0.27269851853751526	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	768	0.21884269826208122	No Hit
AATGACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTT	660	0.18806794381897607	No Hit
AATGCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTC	621	0.17695483804785475	No Hit
CCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	619	0.17638493518779724	No Hit
AACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTG	577	0.16441697512658968	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCT	535	0.1524490150653821	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCT	533	0.1518791122053246	No Hit
TACCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCT	524	0.14931454933506585	No Hit
AACCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCT	490	0.1396262007140883	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	490	0.1396262007140883	No Hit
ACCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTG	452	0.12879804637299572	No Hit
AGCCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCT	369	0.10514707768060935	No Hit
GCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	354	0.10087280623017807	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCT	354	0.10087280623017807	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCGC	25	3.885248E-5	45.000004	25
ACTATTC	25	3.885248E-5	45.000004	28
CATATAG	25	3.885248E-5	45.000004	1
TCCGCAG	25	3.885248E-5	45.000004	1
CTATCGG	25	3.885248E-5	45.000004	2
CTATCCA	25	3.885248E-5	45.000004	41
ATGTGCG	25	3.885248E-5	45.000004	20
TAGCGAT	25	3.885248E-5	45.000004	45
GGCAACG	25	3.885248E-5	45.000004	1
TCTTACG	25	3.885248E-5	45.000004	1
CCGTGGC	25	3.885248E-5	45.000004	20
TAAACGG	25	3.885248E-5	45.000004	2
CCGTGCA	25	3.885248E-5	45.000004	26
CGATTAC	25	3.885248E-5	45.000004	38
CCGCTAG	25	3.885248E-5	45.000004	1
CCGTAGT	25	3.885248E-5	45.000004	27
GTTCGCT	25	3.885248E-5	45.000004	19
CGTAGTG	25	3.885248E-5	45.000004	28
TCTCGGT	25	3.885248E-5	45.000004	43
TCCACGA	25	3.885248E-5	45.000004	34

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content