##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553022_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	350937
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28731082786939	31.0	31.0	34.0	30.0	34.0
2	31.444139546414313	31.0	31.0	34.0	30.0	34.0
3	31.704548109774688	31.0	31.0	34.0	30.0	34.0
4	35.54947184252444	37.0	35.0	37.0	33.0	37.0
5	35.00578166451528	35.0	35.0	37.0	32.0	37.0
6	34.998127869104714	35.0	35.0	37.0	32.0	37.0
7	35.08533155523641	36.0	35.0	37.0	32.0	37.0
8	35.42466311617185	37.0	35.0	37.0	33.0	37.0
9	36.977739594286156	39.0	37.0	39.0	33.0	39.0
10	36.18051388140891	38.0	35.0	39.0	31.0	39.0
11	36.241370958320154	38.0	35.0	39.0	32.0	39.0
12	35.88136617113613	37.0	35.0	39.0	31.0	39.0
13	35.51446270983111	37.0	35.0	39.0	30.0	39.0
14	36.297782792922945	38.0	35.0	40.0	30.0	41.0
15	36.42051137383633	38.0	35.0	40.0	31.0	41.0
16	36.476396048293566	38.0	35.0	40.0	31.0	41.0
17	36.37477951883101	38.0	35.0	40.0	31.0	41.0
18	36.22951697883096	38.0	35.0	40.0	30.0	41.0
19	36.2547665250458	38.0	34.0	40.0	30.0	41.0
20	36.144749627426	38.0	34.0	40.0	30.0	41.0
21	36.21429772295312	38.0	34.0	40.0	30.0	41.0
22	36.19633723431841	38.0	34.0	40.0	30.0	41.0
23	36.05808449949706	38.0	34.0	40.0	30.0	41.0
24	35.58447812570348	37.0	34.0	40.0	29.0	41.0
25	35.709865303459026	37.0	34.0	40.0	29.0	41.0
26	35.649532537179034	37.0	34.0	40.0	29.0	41.0
27	35.45364552612007	37.0	34.0	40.0	29.0	41.0
28	35.36912038343065	37.0	34.0	40.0	27.0	41.0
29	35.094105209766994	37.0	34.0	40.0	27.0	41.0
30	34.99860943702146	37.0	34.0	40.0	27.0	41.0
31	34.849406018744105	37.0	34.0	40.0	26.0	41.0
32	34.756135716667096	37.0	33.0	40.0	25.0	41.0
33	34.732778247947635	37.0	34.0	40.0	25.0	41.0
34	34.728181981381276	37.0	34.0	40.0	25.0	41.0
35	34.54331689163582	37.0	33.0	40.0	25.0	41.0
36	34.59575080427541	37.0	33.0	40.0	25.0	41.0
37	34.36690346130502	37.0	33.0	40.0	24.0	41.0
38	34.33655898352126	37.0	33.0	40.0	24.0	41.0
39	34.174347532463095	37.0	33.0	40.0	23.0	41.0
40	33.916415197029664	36.0	33.0	40.0	23.0	41.0
41	33.91947557538818	36.0	33.0	40.0	23.0	41.0
42	33.7889136796633	36.0	33.0	39.0	23.0	41.0
43	33.765687288601654	36.0	33.0	39.0	23.0	41.0
44	33.705893080524426	36.0	33.0	39.0	22.0	41.0
45	33.4776498345857	36.0	32.0	39.0	22.0	40.0
46	33.44316786203792	36.0	32.0	39.0	22.0	40.0
47	33.426595086867444	36.0	32.0	39.0	22.0	40.0
48	33.398148385607676	36.0	32.0	39.0	22.0	40.0
49	33.1625619413171	36.0	32.0	39.0	20.0	40.0
50	33.003915232648595	35.0	32.0	39.0	19.0	40.0
51	31.191230904692294	34.0	29.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	3.0
12	5.0
13	12.0
14	22.0
15	29.0
16	71.0
17	167.0
18	353.0
19	760.0
20	1292.0
21	1975.0
22	2657.0
23	3300.0
24	3847.0
25	4116.0
26	4866.0
27	5420.0
28	6681.0
29	8188.0
30	10636.0
31	13447.0
32	17485.0
33	23703.0
34	35423.0
35	39835.0
36	38631.0
37	48363.0
38	52618.0
39	27026.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.29463123010683	35.37842974664968	19.78560254404637	25.54133647919712
2	18.08273279819455	37.674568369821365	20.44013597882241	23.802562853161678
3	18.883161365145313	34.6996754403212	21.9167542892314	24.50040890530209
4	18.81505797336844	34.86580212402796	18.925334176789566	27.393805725814037
5	15.646113120018693	37.69821933851375	19.68472973781619	26.970937803651367
6	19.978229710745804	36.98498590915179	21.35055579776427	21.686228582338142
7	74.9325377489407	10.99057665620895	8.280688556635521	5.796197038214836
8	76.80808806138994	8.99335208313743	5.77254606952245	8.426013785950184
9	70.93552403992739	12.021530930052972	11.450203312845325	5.592741717174308
10	34.70879388608212	37.219500936065444	12.326998863043794	15.74470631480864
11	28.303370690465812	26.593662110293298	29.59477057135611	15.508196627884777
12	28.39427019664498	24.999643810712463	31.433277197901617	15.172808794740936
13	23.066533309397414	35.64286467371636	23.673479855358654	17.61712216152757
14	13.897651145362271	37.58680332937251	31.774079108216004	16.741466417049214
15	12.928816283264519	33.19085761831896	34.59538321693068	19.28494288148585
16	15.244046652248125	30.937461709651593	31.991497049327943	21.826994588772344
17	15.586558271142684	30.663053482533904	26.615603370405516	27.1347848759179
18	19.359885107583413	29.853791421252247	26.520144641345883	24.266178829818458
19	19.864534090164334	36.16803015925936	26.005237407283925	17.962198343292386
20	19.964552042104422	33.93230123925377	25.40512969564338	20.69801702299843
21	16.81127951740626	33.52966486862314	25.86760586658004	23.79144974739056
22	15.948446587279197	33.67612990365792	26.608764536084824	23.766658972978057
23	18.055947363771846	32.82241541929179	22.292320274009295	26.82931694292708
24	14.9097986248244	35.52916905313489	26.138594676537387	23.422437645503326
25	16.551118861790005	32.375896528436726	25.083704482570944	25.98928012720232
26	18.642662358201044	35.38840304670069	23.075651755158333	22.893282839939932
27	13.61754388964401	32.89251347107886	25.924311201155763	27.565631438121375
28	17.45697945785141	29.266221572532963	28.20848186426624	25.06831710534939
29	16.313754320576056	30.52941126185042	29.349712341531387	23.807122076042138
30	18.157390072862082	31.014398595759353	24.37275066464921	26.45546066672936
31	20.183394740366502	31.91199560034992	24.2903997013709	23.614209957912674
32	20.919994186990827	30.141022462721228	21.123164556601324	27.815818793686613
33	19.12793464354001	28.576639111863383	27.32570233403716	24.969723910559445
34	15.057118514149263	27.749140159059888	25.65560200263865	31.538139324152198
35	15.39564081302342	30.84998162063276	26.428390280876624	27.32598728546719
36	18.963517668413417	24.905609838802974	32.24424896776345	23.88662352502016
37	15.18762626910243	32.06814898400568	25.112769528433876	27.63145521845801
38	18.77146040457404	28.801465790156065	28.18711050701408	24.239963298255812
39	17.988128923425002	27.159005747470346	28.544154648840102	26.308710680264547
40	20.752442746133923	29.505865725187142	23.85470896485694	25.886982563822
41	16.243086365928928	25.403135035633177	29.817317638208568	28.536460960229327
42	21.35739463208496	28.953914805221448	24.140515249175778	25.548175313517813
43	17.844228451260484	27.00313731524462	28.63305949500908	26.519574738485822
44	20.37174763561551	25.446162701567516	25.629101519645975	28.552988143171
45	21.3964329779989	29.302410404146613	24.400960856222056	24.90019576163243
46	16.66054021092105	27.709816861715918	31.20446119958853	24.4251817277745
47	18.54720362914141	27.06896109558126	26.45745532673956	27.92637994853777
48	19.779618564015763	23.879214787839413	32.61753534110111	23.723631307043714
49	19.271265212844472	22.9821876861089	30.265261286213764	27.481285814832862
50	17.505991103816353	24.711842866383424	26.674873267851495	31.107292761948724
51	17.42734450912842	25.91063353251438	31.55096213850349	25.111059819853704
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5287.0
1	4139.0
2	2991.0
3	2066.0
4	1141.0
5	1060.0
6	979.0
7	1070.0
8	1161.0
9	1350.0
10	1539.0
11	1717.0
12	1895.0
13	2071.5
14	2248.0
15	2232.0
16	2216.0
17	2153.5
18	2091.0
19	2013.5
20	1936.0
21	1922.0
22	1908.0
23	1860.0
24	1812.0
25	1844.0
26	2071.5
27	2267.0
28	2752.5
29	3238.0
30	3746.5
31	4255.0
32	4811.5
33	5368.0
34	5805.0
35	6242.0
36	7348.5
37	8455.0
38	9191.5
39	9928.0
40	11345.0
41	12762.0
42	16898.5
43	21035.0
44	29423.5
45	37812.0
46	39919.0
47	42026.0
48	42699.5
49	43373.0
50	39933.5
51	36494.0
52	31955.0
53	27416.0
54	22831.0
55	18246.0
56	15918.0
57	13590.0
58	11750.5
59	9911.0
60	8785.0
61	7659.0
62	6228.5
63	4798.0
64	3713.0
65	2628.0
66	2101.5
67	1575.0
68	1249.5
69	924.0
70	729.5
71	535.0
72	455.5
73	376.0
74	438.0
75	366.5
76	233.0
77	155.0
78	77.0
79	60.5
80	44.0
81	36.5
82	29.0
83	22.0
84	15.0
85	14.0
86	13.0
87	11.0
88	9.0
89	7.5
90	6.0
91	5.0
92	4.0
93	5.5
94	7.0
95	7.0
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	350937.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.326290858131806
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.47673537672615	33.639554180100845
2	9.386276193813366	7.945725125082056
3	3.3116107278298665	4.205045966251094
4	1.6715787598450562	2.8300691512590843
5	1.0331861680639913	2.186546913003758
6	0.7092330000195948	1.8011521347008865
7	0.5448396445283141	1.6142728885742597
8	0.441057133225881	1.4934650004777945
9	0.3389603538121086	1.2912241072343917
>10	2.703074151705713	23.56986337178366
>50	0.3267413193100358	9.043055132036327
>100	0.045905805192319085	3.622022461251658
>500	0.006075768334277525	1.6555694336140823
>1k	0.004725597593326965	5.1024341346300774
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4564	1.3005183266512224	No Hit
CTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGCT	3525	1.0044537908513496	No Hit
AATCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCT	2897	0.8255042927932934	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGT	2449	0.6978460521404127	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCC	1847	0.5263052912631042	No Hit
AAACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCT	1463	0.4168839421320636	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1112	0.31686599019197176	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTT	957	0.27269851853751526	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	768	0.21884269826208122	No Hit
AATGACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTT	660	0.18806794381897607	No Hit
AATGCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTC	621	0.17695483804785475	No Hit
CCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	619	0.17638493518779724	No Hit
AACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTG	577	0.16441697512658968	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCT	535	0.1524490150653821	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCT	533	0.1518791122053246	No Hit
TACCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCT	524	0.14931454933506585	No Hit
AACCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCT	490	0.1396262007140883	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	490	0.1396262007140883	No Hit
ACCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTG	452	0.12879804637299572	No Hit
AGCCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCT	369	0.10514707768060935	No Hit
GCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	354	0.10087280623017807	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCT	354	0.10087280623017807	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.849514300287516E-4	0.0	0.0	1.250366874966162	0.0
2	2.849514300287516E-4	0.0	0.0	1.7681236233284037	0.0
3	2.849514300287516E-4	0.0	0.0	2.538917241556177	0.0
4	2.849514300287516E-4	0.0	0.0	6.40257368131602	0.0
5	2.849514300287516E-4	0.0	0.0	7.059101776102263	0.0
6	2.849514300287516E-4	0.0	0.0	9.560975331754703	0.0
7	2.849514300287516E-4	0.0	0.0	11.473854281537712	0.0
8	2.849514300287516E-4	0.0	0.0	12.572626995728578	0.0
9	2.849514300287516E-4	0.0	0.0	15.035462205467079	0.0
10	2.849514300287516E-4	0.0	0.0	17.682945941864208	0.0
11	2.849514300287516E-4	0.0	0.0	20.801454392098865	0.0
12	2.849514300287516E-4	0.0	0.0	22.184893584888457	0.0
13	2.849514300287516E-4	0.0	0.0	22.75736100781622	0.0
14	2.849514300287516E-4	0.0	0.0	23.383114348159356	0.0
15	2.849514300287516E-4	0.0	0.0	23.840461393355504	0.0
16	2.849514300287516E-4	0.0	0.0	24.678218597640033	0.0
17	2.849514300287516E-4	0.0	0.0	25.759039371739085	0.0
18	2.849514300287516E-4	0.0	0.0	27.082353812792608	0.0
19	2.849514300287516E-4	0.0	0.0	27.67533773868244	0.0
20	2.849514300287516E-4	0.0	0.0	28.3062202047661	0.0
21	2.849514300287516E-4	0.0	0.0	29.05706722289186	0.0
22	2.849514300287516E-4	0.0	0.0	29.8275758896896	0.0
23	2.849514300287516E-4	0.0	0.0	30.520292816089498	0.0
24	2.849514300287516E-4	0.0	0.0	31.04716801021266	0.0
25	2.849514300287516E-4	0.0	0.0	31.475450009545874	0.0
26	2.849514300287516E-4	0.0	0.0	31.92310870612104	0.0
27	2.849514300287516E-4	0.0	0.0	32.43915574590311	0.0
28	2.849514300287516E-4	0.0	0.0	32.89279842250888	0.0
29	2.849514300287516E-4	0.0	0.0	33.426512450952735	0.0
30	2.849514300287516E-4	0.0	0.0	34.04428715125507	0.0
31	2.849514300287516E-4	0.0	0.0	34.497929827860844	0.0
32	2.849514300287516E-4	0.0	0.0	34.92991619578443	0.0
33	2.849514300287516E-4	0.0	0.0	35.3607627579879	0.0
34	2.849514300287516E-4	0.0	0.0	35.871680672029456	0.0
35	2.849514300287516E-4	0.0	0.0	36.35752286022847	0.0
36	2.849514300287516E-4	0.0	0.0	36.79007913101212	0.0
37	2.849514300287516E-4	0.0	0.0	37.21921598463542	0.0
38	2.849514300287516E-4	0.0	0.0	37.67798778698171	0.0
39	2.849514300287516E-4	0.0	0.0	38.321978018846686	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCGC	25	3.885248E-5	45.000004	25
ACTATTC	25	3.885248E-5	45.000004	28
CATATAG	25	3.885248E-5	45.000004	1
TCCGCAG	25	3.885248E-5	45.000004	1
CTATCGG	25	3.885248E-5	45.000004	2
CTATCCA	25	3.885248E-5	45.000004	41
ATGTGCG	25	3.885248E-5	45.000004	20
TAGCGAT	25	3.885248E-5	45.000004	45
GGCAACG	25	3.885248E-5	45.000004	1
TCTTACG	25	3.885248E-5	45.000004	1
CCGTGGC	25	3.885248E-5	45.000004	20
TAAACGG	25	3.885248E-5	45.000004	2
CCGTGCA	25	3.885248E-5	45.000004	26
CGATTAC	25	3.885248E-5	45.000004	38
CCGCTAG	25	3.885248E-5	45.000004	1
CCGTAGT	25	3.885248E-5	45.000004	27
GTTCGCT	25	3.885248E-5	45.000004	19
CGTAGTG	25	3.885248E-5	45.000004	28
TCTCGGT	25	3.885248E-5	45.000004	43
TCCACGA	25	3.885248E-5	45.000004	34
>>END_MODULE