SRR3553021_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553021_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	313472
Sequences flagged as poor quality	0
Sequence length	46
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5283	1.6853179869334423	No Hit
CTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTT	3626	1.1567221314822378	No Hit
AATCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGT	2249	0.7174484483462638	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTAT	1881	0.6000535933033891	No Hit
AAACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGT	1389	0.4431017762351981	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTCTCTCATCGT	1351	0.43097948142098813	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1263	0.4029067986933442	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCC	954	0.30433340138832177	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	857	0.2733896488362597	No Hit
CCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCT	804	0.2564822376480196	No Hit
TACCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGT	544	0.17354022049816253	No Hit
ACCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTC	540	0.17226418946508779	No Hit
AACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTC	537	0.17130716619028175	No Hit
AATGCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCG	515	0.16428899550837076	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	512	0.16333197223356472	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGC	511	0.16301296447529603	No Hit
AATGACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCC	508	0.16205594120049	No Hit
TACATTGGGGTTGTTTCTGTTGCTGTTGCTCTTGTTGTTTTATATA	471	0.1502526541445488	No Hit
AACCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGT	431	0.13749234381380154	No Hit
GCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCT	396	0.1263270722743977	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGC	390	0.12441302572478563	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	368	0.11739485504287464	No Hit
TCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCT	367	0.11707584728460596	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGC	359	0.11452378521845652	No Hit
AGCCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGT	334	0.10654859126173948	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAT	25	7.781669E-5	40.000004	30
TCGTTGA	25	7.781669E-5	40.000004	24
TGGGTGA	25	7.781669E-5	40.000004	5
CGTTGAT	25	7.781669E-5	40.000004	25
ATTAACG	25	7.781669E-5	40.000004	1
TTCAACT	25	7.781669E-5	40.000004	24
CGCGATG	25	7.781669E-5	40.000004	1
GTAGGGC	50	7.6397555E-11	40.000004	4
AAGCGGT	25	7.781669E-5	40.000004	27
TCGATCA	25	7.781669E-5	40.000004	17
GGCCCAC	25	7.781669E-5	40.000004	27
ATCGTTG	25	7.781669E-5	40.000004	23
CATTATG	25	7.781669E-5	40.000004	1
TGCATCG	25	7.781669E-5	40.000004	23
CCACTAG	25	7.781669E-5	40.000004	1
TCTCGAG	25	7.781669E-5	40.000004	1
GAAAACG	25	7.781669E-5	40.000004	1
GGGCCTA	25	7.781669E-5	40.000004	7
CTCGATC	25	7.781669E-5	40.000004	16
CTCGAGG	50	7.6397555E-11	40.000004	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content