##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553021_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	313472
Sequences flagged as poor quality	0
Sequence length	46
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.108408406492448	31.0	31.0	34.0	28.0	34.0
2	31.33481140261331	31.0	31.0	34.0	28.0	34.0
3	31.600771360759495	31.0	31.0	34.0	30.0	34.0
4	35.51278583095141	37.0	35.0	37.0	33.0	37.0
5	34.93252028889343	35.0	35.0	37.0	32.0	37.0
6	34.983000076561865	35.0	35.0	37.0	32.0	37.0
7	34.741750459371175	35.0	35.0	37.0	32.0	37.0
8	35.36366884442629	37.0	35.0	37.0	33.0	37.0
9	36.759187423438135	39.0	37.0	39.0	32.0	39.0
10	36.245447759289505	38.0	35.0	39.0	32.0	39.0
11	36.47719732543896	38.0	35.0	39.0	32.0	39.0
12	36.09626378113516	37.0	35.0	39.0	32.0	39.0
13	35.66910282258065	37.0	35.0	39.0	30.0	39.0
14	36.66018017558187	38.0	35.0	40.0	31.0	41.0
15	36.68312959371172	38.0	35.0	40.0	31.0	41.0
16	36.71839271131074	38.0	35.0	40.0	31.0	41.0
17	36.66727809820335	38.0	35.0	40.0	32.0	41.0
18	36.52133842895059	38.0	35.0	40.0	31.0	41.0
19	36.46638934258881	38.0	35.0	40.0	31.0	41.0
20	36.3513232441813	38.0	35.0	40.0	30.0	41.0
21	36.48165386382197	38.0	35.0	40.0	31.0	41.0
22	36.48297136586362	38.0	35.0	40.0	31.0	41.0
23	36.329244717231525	38.0	35.0	40.0	31.0	41.0
24	35.78112239689669	38.0	34.0	40.0	29.0	41.0
25	36.01746567476521	38.0	34.0	40.0	30.0	41.0
26	35.99111563393222	38.0	34.0	40.0	30.0	41.0
27	35.78971646590445	38.0	34.0	40.0	30.0	41.0
28	35.88512849632503	38.0	34.0	40.0	30.0	41.0
29	35.52576306655778	38.0	34.0	40.0	29.0	41.0
30	35.58266767047775	38.0	34.0	40.0	29.0	41.0
31	35.45646501122907	38.0	34.0	40.0	29.0	41.0
32	35.3113388117599	38.0	34.0	40.0	27.0	41.0
33	35.3206251276031	38.0	34.0	40.0	28.0	41.0
34	35.21399997447938	38.0	34.0	40.0	27.0	41.0
35	35.06408227848101	38.0	34.0	40.0	27.0	41.0
36	35.16749502347897	38.0	34.0	40.0	27.0	41.0
37	34.92109981114741	38.0	34.0	40.0	25.0	41.0
38	34.88491157104941	37.0	34.0	40.0	25.0	41.0
39	34.789362367292775	37.0	34.0	40.0	25.0	41.0
40	34.52724964271131	37.0	33.0	40.0	24.0	41.0
41	34.56359738668844	37.0	33.0	40.0	24.0	41.0
42	34.47008026235198	37.0	33.0	40.0	24.0	41.0
43	34.462047646998776	37.0	33.0	40.0	24.0	41.0
44	34.46539084830543	37.0	33.0	40.0	24.0	41.0
45	34.29527358105349	37.0	33.0	40.0	23.0	41.0
46	33.2042670477746	35.0	32.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	4.0
11	3.0
12	8.0
13	10.0
14	9.0
15	31.0
16	43.0
17	92.0
18	170.0
19	349.0
20	587.0
21	1053.0
22	1548.0
23	2182.0
24	2666.0
25	3231.0
26	3739.0
27	4280.0
28	4882.0
29	6161.0
30	7885.0
31	10272.0
32	13838.0
33	19168.0
34	29669.0
35	36452.0
36	34869.0
37	46015.0
38	54573.0
39	29680.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.406517966516944	34.998660167415274	19.65693905675786	25.93788280930992
2	18.48139546753777	37.17429307880767	19.66906135157207	24.675250102082483
3	19.048591261739485	34.69911188240098	21.864791751735403	24.387505104124134
4	18.705338913842382	34.545669150673746	19.160562984891794	27.588428950592082
5	15.790246018783177	37.40940179665169	19.99094017966517	26.809412004899958
6	20.172774601878317	36.50310075541037	21.667644957125358	21.656479685585953
7	73.98204624336464	11.07531135157207	8.896807370355248	6.045835034708044
8	76.01795375663536	8.961884953042059	6.213952123315639	8.80620916700694
9	70.28155624744794	12.021807370355248	12.027549510004084	5.669086872192731
10	34.670720191915066	36.66643272764394	12.714373213556554	15.948473866884443
11	27.646488362596976	26.207763372805225	30.137938954675374	16.007809309922415
12	28.30460136790527	24.872715904450796	31.576663944467132	15.246018783176806
13	22.609355859534507	35.902728154348715	24.101036137198857	17.386879848917925
14	13.584945385871785	37.03807676602695	32.05070947325439	17.326268374846876
15	12.726495508370764	33.06643017558187	34.45188086974275	19.755193446304613
16	15.00070181706819	30.923017047774604	32.11706308697428	21.959218048182933
17	15.293231931400571	30.661749693752554	26.784529399755	27.260488975091874
18	18.813801551653736	29.468660677827685	26.83620865659453	24.88132911392405
19	19.72488770926909	36.14900214373213	26.239345140873827	17.88676500612495
20	19.55358054307881	33.84512811351572	26.012530624744794	20.58876071866068
21	16.52204981625153	33.513041037158025	26.328986320947322	23.635922825643117
22	16.044176194365047	33.49358156390363	26.89171600653328	23.570526235198038
23	17.773517251939566	33.08716567986934	22.478243670886076	26.661073397305024
24	14.691583299305838	35.51226265822785	26.657564311964066	23.138589730502247
25	16.583618313597388	32.077825132707225	25.541356165781952	25.79720038791343
26	18.75733717844018	35.25673744385463	23.402409146590443	22.583516231114743
27	13.277421906900777	33.12799867292773	26.14715189873418	27.447427521437323
28	17.227375969783584	29.20579828501429	28.706232135565536	24.860593609636584
29	16.036839015924865	30.626977848101266	29.38061453654553	23.955568599428336
30	17.723433033891382	30.801156084115966	24.642711310739077	26.83269957125357
31	19.98105093915884	31.7572223356472	24.698537668436096	23.56318905675786
32	20.890861065741117	29.883370763576973	21.390108207431606	27.835659963250308
33	18.804550326663943	28.476865557370356	27.644574316047365	25.074009799918333
34	15.002615863617802	27.573435585953447	25.88014240506329	31.543806145365455
35	15.009315026541445	30.94981369946917	26.784210391996734	27.25666088199265
36	18.77360657411188	24.91514393630053	32.169699367088604	24.141550122498977
37	15.113630563495303	31.904284912209064	25.271794610044918	27.710289914250712
38	18.610912617394856	29.038319211923238	28.340330236831356	24.01043793385055
39	17.589768783176808	27.191583299305837	28.761420477746018	26.457227439771337
40	20.72370100040833	29.470574724377297	23.859228256431198	25.946496018783176
41	16.085328195181706	25.367177929767255	30.03266639444671	28.51482748060433
42	20.99964271131074	29.144229787668436	24.311262249897915	25.544865251122907
43	17.5195870763577	26.728703042057983	29.127322376480198	26.624387505104124
44	20.40947835851368	25.349313495304205	25.895773785218456	28.345434360963655
45	21.208273785218456	29.12955543078808	24.78498877092691	24.877182013066555
46	16.387428542262146	27.697210596161696	31.45033687219273	24.465023989383422
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6027.0
1	4692.0
2	3357.0
3	2332.0
4	1307.0
5	1403.0
6	1499.0
7	1499.0
8	1511.5
9	1524.0
10	1581.5
11	1639.0
12	1712.0
13	1785.0
14	1880.5
15	1976.0
16	1937.5
17	1899.0
18	1871.5
19	1844.0
20	1844.0
21	1783.5
22	1723.0
23	1785.0
24	1847.0
25	1935.0
26	2023.0
27	2086.0
28	2149.0
29	2599.0
30	3049.0
31	3049.0
32	3898.5
33	4748.0
34	5309.0
35	5870.0
36	7313.5
37	8757.0
38	8751.5
39	8746.0
40	12304.5
41	15863.0
42	18555.0
43	21247.0
44	21247.0
45	29182.0
46	37117.0
47	41883.0
48	46649.0
49	43426.5
50	40204.0
51	33878.5
52	27553.0
53	23384.5
54	19216.0
55	16471.5
56	13727.0
57	13727.0
58	12246.5
59	10766.0
60	9398.5
61	8031.0
62	6391.5
63	4752.0
64	3726.0
65	2700.0
66	2147.5
67	1595.0
68	1252.5
69	910.0
70	910.0
71	678.0
72	446.0
73	405.0
74	364.0
75	274.5
76	185.0
77	211.5
78	238.0
79	151.5
80	65.0
81	65.0
82	51.0
83	37.0
84	27.5
85	18.0
86	11.0
87	4.0
88	4.0
89	4.0
90	5.5
91	7.0
92	4.5
93	2.0
94	2.0
95	2.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	313472.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.69557584666936
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.13373621311096	30.618680761845336
2	10.071392303004751	7.995794340909741
3	3.621934103830899	4.313242797907736
4	1.9552323539159244	3.104562968109258
5	1.2131697705703939	2.4078736321281764
6	0.8873187397939417	2.1133577001396895
7	0.6468365396201938	1.7973584243223386
8	0.5330960277050936	1.6929243041060555
9	0.37582527083038936	1.3426740489008497
>10	3.2170875904348923	26.161580522982252
>50	0.27420648628117955	6.837565431062273
>100	0.056454276587301684	4.147422987143541
>500	0.00806489665532881	2.0111239834222867
>1k	0.004838937993197286	3.764534689758464
>5k	8.064896655328813E-4	1.691303407262009
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5283	1.6853179869334423	No Hit
CTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTT	3626	1.1567221314822378	No Hit
AATCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGT	2249	0.7174484483462638	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTAT	1881	0.6000535933033891	No Hit
AAACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGT	1389	0.4431017762351981	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTCTCTCATCGT	1351	0.43097948142098813	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1263	0.4029067986933442	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCC	954	0.30433340138832177	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	857	0.2733896488362597	No Hit
CCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCT	804	0.2564822376480196	No Hit
TACCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGT	544	0.17354022049816253	No Hit
ACCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTC	540	0.17226418946508779	No Hit
AACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTC	537	0.17130716619028175	No Hit
AATGCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCG	515	0.16428899550837076	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	512	0.16333197223356472	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGC	511	0.16301296447529603	No Hit
AATGACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCC	508	0.16205594120049	No Hit
TACATTGGGGTTGTTTCTGTTGCTGTTGCTCTTGTTGTTTTATATA	471	0.1502526541445488	No Hit
AACCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGT	431	0.13749234381380154	No Hit
GCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCT	396	0.1263270722743977	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGC	390	0.12441302572478563	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	368	0.11739485504287464	No Hit
TCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCT	367	0.11707584728460596	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGC	359	0.11452378521845652	No Hit
AGCCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGT	334	0.10654859126173948	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.2578475908534095	0.0
2	0.0	0.0	0.0	1.8923540220498163	0.0
3	0.0	0.0	0.0	2.746656798693344	0.0
4	0.0	0.0	0.0	6.653225806451613	0.0
5	0.0	0.0	0.0	7.304958656594528	0.0
6	0.0	0.0	0.0	9.90231982441813	0.0
7	0.0	0.0	0.0	11.821789505920783	0.0
8	0.0	0.0	0.0	12.951714985708453	0.0
9	0.0	0.0	0.0	15.360861576153532	0.0
10	0.0	0.0	0.0	18.066366374030217	0.0
11	0.0	0.0	0.0	21.208273785218456	0.0
12	0.0	0.0	0.0	22.62722029399755	0.0
13	0.0	0.0	0.0	23.20302929767252	0.0
14	0.0	0.0	0.0	23.81999030216415	0.0
15	0.0	0.0	0.0	24.272981318905675	0.0
16	0.0	0.0	0.0	25.139725398121683	0.0
17	0.0	0.0	0.0	26.268055839118006	0.0
18	0.0	0.0	0.0	27.680941200489997	0.0
19	0.0	0.0	0.0	28.317042670477747	0.0
20	0.0	0.0	0.0	28.942935892200897	0.0
21	0.0	0.0	0.0	29.68494793793385	0.0
22	0.0	0.0	0.0	30.499055737035526	0.0
23	0.0	0.0	0.0	31.245852899142506	0.0
24	0.0	0.0	0.0	31.785933033891386	0.0
25	0.0	0.0	0.0	32.263168640261334	0.0
26	0.0	0.0	0.0	32.75220753368722	0.0
27	0.0	0.0	0.0	33.268681094324215	0.0
28	0.0	0.0	0.0	33.70731676194365	0.0
29	0.0	0.0	0.0	34.24452582686811	0.0
30	0.0	0.0	0.0	34.84872652102899	0.0
31	0.0	0.0	0.0	35.34031747652103	0.0
32	0.0	0.0	0.0	35.76587382605145	0.0
33	0.0	0.0	0.0	36.20769957125357	0.0
34	0.0	0.0	0.0	36.721621069824415	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAT	25	7.781669E-5	40.000004	30
TCGTTGA	25	7.781669E-5	40.000004	24
TGGGTGA	25	7.781669E-5	40.000004	5
CGTTGAT	25	7.781669E-5	40.000004	25
ATTAACG	25	7.781669E-5	40.000004	1
TTCAACT	25	7.781669E-5	40.000004	24
CGCGATG	25	7.781669E-5	40.000004	1
GTAGGGC	50	7.6397555E-11	40.000004	4
AAGCGGT	25	7.781669E-5	40.000004	27
TCGATCA	25	7.781669E-5	40.000004	17
GGCCCAC	25	7.781669E-5	40.000004	27
ATCGTTG	25	7.781669E-5	40.000004	23
CATTATG	25	7.781669E-5	40.000004	1
TGCATCG	25	7.781669E-5	40.000004	23
CCACTAG	25	7.781669E-5	40.000004	1
TCTCGAG	25	7.781669E-5	40.000004	1
GAAAACG	25	7.781669E-5	40.000004	1
GGGCCTA	25	7.781669E-5	40.000004	7
CTCGATC	25	7.781669E-5	40.000004	16
CTCGAGG	50	7.6397555E-11	40.000004	2
>>END_MODULE