FastQCFastQC Report
Sat 18 Jun 2016
SRR3553020_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553020_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805063
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCT57230.7108760432413365No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCC43390.5389640313863636No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGT42320.5256731460767666No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40420.5020725086111274No Hit
CTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCTGCT30310.37649227451764644No Hit
AACTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCTG10910.13551734460532902No Hit
AATGACTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTT10040.12471073692369417No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9110.1131588459536707No Hit
AATGATCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCT8600.10682393800236752No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG207.0320256E-445.01
CGAACGC207.0320256E-445.022
CATGCGT207.0320256E-445.025
ATTCGGC253.8897604E-545.017
TTCGTAA253.8897604E-545.018
TCGATTG207.0320256E-445.013
TAACGCG900.045.01
GCGCACA207.0320256E-445.031
CGCCTAT207.0320256E-445.032
CATAGCG207.0320256E-445.01
CGTAGGT207.0320256E-445.015
TCGTAAT253.8897604E-545.019
CGTAATT253.8897604E-545.020
ACGTACG207.0320256E-445.01
CGCACGT207.0320256E-445.022
CGCACAT207.0320256E-445.032
CACGTTA253.8897604E-545.037
CGACTAT253.8897604E-545.03
CGCAACA207.0320256E-445.031
TTCGATC453.8380676E-1045.013