##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553020_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	805063
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.70828121525893	31.0	31.0	34.0	30.0	34.0
2	31.876884169313456	33.0	31.0	34.0	30.0	34.0
3	32.06496758638765	33.0	31.0	34.0	30.0	34.0
4	35.8246298736869	37.0	35.0	37.0	35.0	37.0
5	35.38934220054828	37.0	35.0	37.0	33.0	37.0
6	35.4236823701996	37.0	35.0	37.0	33.0	37.0
7	35.49885040052766	37.0	35.0	37.0	33.0	37.0
8	35.79882195554882	37.0	35.0	37.0	35.0	37.0
9	37.4300880800633	39.0	37.0	39.0	35.0	39.0
10	36.777659636575024	39.0	37.0	39.0	32.0	39.0
11	36.683365152789285	39.0	35.0	39.0	32.0	39.0
12	36.33526817155924	38.0	35.0	39.0	32.0	39.0
13	36.15519282341879	38.0	35.0	39.0	32.0	39.0
14	37.138943163454286	39.0	36.0	40.0	32.0	41.0
15	37.16029056111137	39.0	35.0	40.0	32.0	41.0
16	37.19513876553761	39.0	35.0	40.0	32.0	41.0
17	37.07394203931866	39.0	35.0	40.0	32.0	41.0
18	36.97007190741594	38.0	35.0	40.0	32.0	41.0
19	37.06813628250212	39.0	35.0	40.0	32.0	41.0
20	36.97732102953434	39.0	35.0	40.0	31.0	41.0
21	37.06154797823276	39.0	35.0	40.0	32.0	41.0
22	37.07258810801142	39.0	35.0	40.0	32.0	41.0
23	36.95630776721822	39.0	35.0	40.0	32.0	41.0
24	36.598837606497874	38.0	35.0	40.0	31.0	41.0
25	36.716409771657624	38.0	35.0	40.0	31.0	41.0
26	36.64823374071346	38.0	35.0	40.0	31.0	41.0
27	36.5396335442071	38.0	35.0	40.0	31.0	41.0
28	36.46208930232789	38.0	35.0	40.0	31.0	41.0
29	36.19836832645395	38.0	35.0	40.0	30.0	41.0
30	36.13561671571045	38.0	35.0	40.0	30.0	41.0
31	36.07570587643451	38.0	35.0	40.0	30.0	41.0
32	35.933210195972244	38.0	35.0	40.0	30.0	41.0
33	35.94403916215253	38.0	35.0	40.0	30.0	41.0
34	35.87073309790662	38.0	35.0	40.0	30.0	41.0
35	35.678042587971376	38.0	34.0	40.0	29.0	41.0
36	35.63909408332019	38.0	34.0	40.0	29.0	41.0
37	35.39264256337703	38.0	34.0	40.0	27.0	41.0
38	35.34219185330837	38.0	34.0	40.0	27.0	41.0
39	35.20410824991336	38.0	34.0	40.0	27.0	41.0
40	35.08538213779543	38.0	34.0	40.0	26.0	41.0
41	35.088169497294004	37.0	34.0	40.0	27.0	41.0
42	35.03076529414468	37.0	34.0	40.0	27.0	41.0
43	34.97892338860437	37.0	34.0	40.0	26.0	41.0
44	34.817975736060404	37.0	34.0	40.0	26.0	41.0
45	34.60111072052746	37.0	33.0	40.0	25.0	41.0
46	34.539032349021134	37.0	33.0	40.0	25.0	41.0
47	34.420808060984044	36.0	33.0	40.0	25.0	41.0
48	34.378987731394936	36.0	33.0	40.0	25.0	41.0
49	34.219158003783555	36.0	33.0	40.0	24.0	41.0
50	34.07388241665559	36.0	33.0	39.0	24.0	41.0
51	32.45889824771478	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	8.0
11	12.0
12	15.0
13	32.0
14	48.0
15	72.0
16	156.0
17	298.0
18	581.0
19	1171.0
20	1860.0
21	2750.0
22	3816.0
23	4720.0
24	5738.0
25	6737.0
26	7838.0
27	9306.0
28	11483.0
29	14346.0
30	18453.0
31	24328.0
32	32345.0
33	45383.0
34	71817.0
35	78192.0
36	82556.0
37	112703.0
38	151444.0
39	116841.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.134913665141735	31.850178184812865	19.388668961311105	26.626239188734296
2	24.466780860628297	32.43534978007933	19.206074555655892	23.891794803636486
3	21.636691786853948	30.86454103591893	21.64128770046568	25.857479476761448
4	22.159880655302754	31.63913880031749	19.47065012303385	26.730330421345904
5	18.474206366458276	35.65236509440876	17.882699863240518	27.990728675892445
6	21.13225921449626	36.94481053035601	20.315677158185135	21.607253096962598
7	83.6207849572021	7.660270065820936	4.787824058489833	3.9311209184871245
8	84.89807629961878	5.582047616149295	3.9559636947667447	5.563912389465172
9	80.62760305715206	7.747840852206597	6.892255637136473	4.732300453504881
10	43.07923727708266	28.36076182857739	12.731053346135642	15.828947548204303
11	33.05256855674649	28.316044831274073	20.28151834080066	18.349868271178778
12	30.87745927958433	24.978914693632674	25.635757698465834	18.507868328317162
13	26.743745520536905	29.874929042820252	23.020185997866005	20.36113943877684
14	20.615405253998755	30.762685653172483	28.177422139633794	20.444486953194968
15	20.390453914786793	28.91835794217347	29.233612773161855	21.457575369877883
16	23.62063590054443	26.49258505234994	28.71750409595274	21.169274951152893
17	22.68816229288888	27.541074425231315	25.39714283230008	24.37362044957972
18	24.50007018084299	27.404811797337597	25.08288171236288	23.012236309456526
19	24.852464962369407	30.162732606019656	24.183573211040628	20.801229220570317
20	25.862323818135973	30.249433895235526	24.430386193378656	19.457856093249845
21	24.425044996478537	28.189967741655003	26.240182445348996	21.144804816517464
22	24.193013466026883	27.005836810286898	25.58433315156702	23.2168165721192
23	23.782486588006154	28.82109847303876	23.399286763893013	23.997128175062077
24	22.085849181989484	30.524567642532325	25.489309532297472	21.90027364318072
25	23.639267982754145	27.6927395744184	25.015682002526514	23.652310440300944
26	23.724478705393242	30.521089653853178	22.322228198290073	23.43220344246351
27	21.546264081196128	29.05039729809965	25.818848959646633	23.58448966105758
28	24.1135165819321	28.523854654853096	25.729787606684194	21.632841156530606
29	22.80206642213094	29.966723101173447	24.974194566139545	22.25701591055607
30	25.59414604819747	28.675395590158782	25.1340578314989	20.596400530144845
31	28.38821309636637	28.335049555127984	22.01864947215311	21.258087876352533
32	26.15621386152388	28.845320179911386	23.224393618884484	21.77407233968025
33	26.67592474129354	27.135764530229313	24.179846794598685	22.008463933878467
34	23.036582230210556	28.42274455539504	24.8002951321822	23.7403780822122
35	23.719758577900116	30.371536140649862	25.574520254936573	20.334185026513452
36	26.98459623656782	28.130593506346706	24.350516667639678	20.534293589445795
37	23.68584818827843	31.37145788590458	22.078768990749793	22.863924935067192
38	25.12933770400577	28.920221150394443	24.19947258785958	21.7509685577402
39	22.841442222534138	27.620571309326102	25.061144283118214	24.476842185021543
40	24.56093498272806	27.780558788566857	24.247419146079253	23.41108708262583
41	21.95008340962136	25.339259163568567	27.48120333439743	25.229454092412645
42	24.831597030294525	27.248426520657386	24.104945824115628	23.815030624932458
43	24.36678868610283	25.4874463240765	25.55154068687792	24.594224302942752
44	23.927071545953545	26.21769973281594	24.251766631928184	25.603462089302326
45	24.980777901853642	27.477228490192694	24.508765152540857	23.033228455412807
46	23.19184958195818	25.723949554258486	27.937937776298256	23.146263087485078
47	22.79945793062158	27.953837153117213	25.46074033957591	23.785964576685302
48	23.43145815917512	25.826053364767727	28.53081063221139	22.211677843845763
49	24.385296554431143	24.836814013313244	27.26469853912054	23.51319089313507
50	22.378497086563414	26.320051971087977	25.330688405752095	25.970762536596514
51	22.190064628482492	26.29359441435018	27.514741082374922	24.001599874792408
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4897.0
1	3903.5
2	2910.0
3	2086.5
4	1263.0
5	1282.0
6	1301.0
7	1460.0
8	1619.0
9	1806.5
10	1994.0
11	2293.5
12	2593.0
13	2528.0
14	2463.0
15	2435.0
16	2407.0
17	2161.0
18	1915.0
19	1896.0
20	1877.0
21	2206.0
22	2535.0
23	2467.0
24	2399.0
25	3245.0
26	4781.5
27	5472.0
28	6622.5
29	7773.0
30	9063.0
31	10353.0
32	11927.0
33	13501.0
34	15626.0
35	17751.0
36	20040.5
37	22330.0
38	23229.0
39	24128.0
40	28393.5
41	32659.0
42	37366.0
43	42073.0
44	52884.0
45	63695.0
46	64567.5
47	65440.0
48	65441.0
49	65442.0
50	65762.5
51	66083.0
52	62664.0
53	59245.0
54	53893.5
55	48542.0
56	45632.5
57	42723.0
58	40565.0
59	38407.0
60	35039.5
61	31672.0
62	28927.0
63	26182.0
64	23049.0
65	19916.0
66	18128.5
67	16341.0
68	14443.0
69	12545.0
70	11933.0
71	11321.0
72	10082.5
73	8844.0
74	7720.5
75	5570.0
76	4543.0
77	3975.0
78	3407.0
79	2503.5
80	1600.0
81	1408.5
82	1217.0
83	813.5
84	410.0
85	343.0
86	276.0
87	235.0
88	194.0
89	132.0
90	70.0
91	47.5
92	25.0
93	20.5
94	16.0
95	9.5
96	3.0
97	2.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	805063.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.84892156369884
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.55142478457785	20.304696179733416
2	8.51091443865482	4.399959195202808
3	2.9001152124406864	2.248945519562073
4	1.3938461477596875	1.4411767898121577
5	0.8865593388019434	1.1458301405128068
6	0.5425121657756432	0.8414012652292189
7	0.4384867067895599	0.7934085943369562
8	0.336319054297491	0.6954787883930572
9	0.29189212093098	0.6790586885105938
>10	4.262032656517673	28.12915295487538
>50	1.7135745266856695	30.266313937647077
>100	0.16604778512096144	5.592155451639701
>500	0.0028961822986214203	0.5350222578957816
>1k	0.0028961822986214203	2.2133301848911544
>5k	4.826970497702367E-4	0.714070051757826
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCT	5723	0.7108760432413365	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCC	4339	0.5389640313863636	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGT	4232	0.5256731460767666	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4042	0.5020725086111274	No Hit
CTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCTGCT	3031	0.37649227451764644	No Hit
AACTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCTG	1091	0.13551734460532902	No Hit
AATGACTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTT	1004	0.12471073692369417	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	911	0.1131588459536707	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCT	860	0.10682393800236752	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2421388139810177E-4	0.0	0.0	0.4689074022778342	0.0
2	1.2421388139810177E-4	0.0	0.0	0.5936181392015283	0.0
3	1.2421388139810177E-4	0.0	0.0	0.9162015891923986	0.0
4	1.2421388139810177E-4	0.0	0.0	2.391241430794857	0.0
5	1.2421388139810177E-4	0.0	0.0	2.590356282676014	0.0
6	1.2421388139810177E-4	0.0	0.0	3.4023424253754055	0.0
7	1.2421388139810177E-4	0.0	0.0	3.9981964144420994	0.0
8	1.2421388139810177E-4	0.0	0.0	4.362764156345528	0.0
9	1.2421388139810177E-4	0.0	0.0	5.4466544854253645	0.0
10	1.2421388139810177E-4	0.0	0.0	6.243362320712789	0.0
11	1.2421388139810177E-4	0.0	0.0	7.687969761372712	0.0
12	1.2421388139810177E-4	0.0	0.0	8.144083133866543	0.0
13	1.2421388139810177E-4	0.0	0.0	8.368164975908718	0.0
14	1.2421388139810177E-4	0.0	0.0	8.638827023475182	0.0
15	1.2421388139810177E-4	0.0	0.0	8.821297215248993	0.0
16	1.2421388139810177E-4	0.0	0.0	9.133446699202423	0.0
17	1.2421388139810177E-4	0.0	0.0	9.497766013343055	0.0
18	1.2421388139810177E-4	0.0	0.0	9.961829074246364	0.0
19	1.2421388139810177E-4	0.0	0.0	10.185662488525743	0.0
20	2.4842776279620355E-4	0.0	0.0	10.456945605499197	0.0
21	2.4842776279620355E-4	0.0	0.0	10.739283757917082	0.0
22	2.4842776279620355E-4	0.0	0.0	11.037148645509731	0.0
23	2.4842776279620355E-4	0.0	0.0	11.34134844105368	0.0
24	2.4842776279620355E-4	0.0	0.0	11.55089725897228	0.0
25	2.4842776279620355E-4	0.0	0.0	11.736597011662441	0.0
26	2.4842776279620355E-4	0.0	0.0	11.917079781333884	0.0
27	2.4842776279620355E-4	0.0	0.0	12.147496531327363	0.0
28	2.4842776279620355E-4	0.0	0.0	12.348723019192287	0.0
29	2.4842776279620355E-4	0.0	0.0	12.56448253118079	0.0
30	2.4842776279620355E-4	0.0	0.0	12.829679167965736	0.0
31	2.4842776279620355E-4	0.0	0.0	13.015627348418695	0.0
32	2.4842776279620355E-4	0.0	0.0	13.230268935474616	0.0
33	2.4842776279620355E-4	0.0	0.0	13.45050014719345	0.0
34	2.4842776279620355E-4	0.0	0.0	13.654956195974725	0.0
35	2.4842776279620355E-4	0.0	0.0	13.869100927505052	0.0
36	2.4842776279620355E-4	0.0	0.0	14.057533385585973	0.0
37	2.4842776279620355E-4	0.0	0.0	14.276398244609428	0.0
38	2.4842776279620355E-4	0.0	0.0	14.516006821826366	0.0
39	2.4842776279620355E-4	0.0	0.0	14.862936192571265	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	20	7.0320256E-4	45.0	1
CGAACGC	20	7.0320256E-4	45.0	22
CATGCGT	20	7.0320256E-4	45.0	25
ATTCGGC	25	3.8897604E-5	45.0	17
TTCGTAA	25	3.8897604E-5	45.0	18
TCGATTG	20	7.0320256E-4	45.0	13
TAACGCG	90	0.0	45.0	1
GCGCACA	20	7.0320256E-4	45.0	31
CGCCTAT	20	7.0320256E-4	45.0	32
CATAGCG	20	7.0320256E-4	45.0	1
CGTAGGT	20	7.0320256E-4	45.0	15
TCGTAAT	25	3.8897604E-5	45.0	19
CGTAATT	25	3.8897604E-5	45.0	20
ACGTACG	20	7.0320256E-4	45.0	1
CGCACGT	20	7.0320256E-4	45.0	22
CGCACAT	20	7.0320256E-4	45.0	32
CACGTTA	25	3.8897604E-5	45.0	37
CGACTAT	25	3.8897604E-5	45.0	3
CGCAACA	20	7.0320256E-4	45.0	31
TTCGATC	45	3.8380676E-10	45.0	13
>>END_MODULE