FastQCFastQC Report
Sat 18 Jun 2016
SRR3553019_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553019_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708174
Sequences flagged as poor quality0
Sequence length46
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47620.6724336109487217No Hit
AATCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGT38090.5378621638184966No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGAGATCATCGTAT29420.4154346248238427No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGAGATCATCGT29120.4111983777998062No Hit
CTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTT26420.3730721545834781No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11050.1560350987186765No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9410.13287694832061048No Hit
AACTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTC8850.12496928720907574No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG257.79162E-540.01
CGAACTC200.001255442540.016
GTACCAC200.001255442540.09
CGACGAA200.001255442540.06
TTAGCCG257.79162E-540.01
GCGAAAT200.001255442540.030
ACGCAAC200.001255442540.026
CTATACG304.8648653E-640.01
ATGCTCG257.79162E-540.01
ATTCGGA200.001255442540.018
TAACGGT200.001255442540.036
CGACAAT900.040.020
ATGTCGC200.001255442540.039
CGTTAAG304.8648653E-640.01
TACGCAA200.001255442540.025
GTTAACG304.8648653E-640.01
CATAGCG451.2205419E-940.01
CGTACTG507.8216544E-1140.01
AATTCGG200.001255442540.02
TTACGGT257.79162E-540.028
Produced by FastQC (version 0.11.5)