##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553019_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	708174
Sequences flagged as poor quality	0
Sequence length	46
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.557163917342347	31.0	31.0	34.0	30.0	34.0
2	31.78824272000949	33.0	31.0	34.0	30.0	34.0
3	31.973078650162247	33.0	31.0	34.0	30.0	34.0
4	35.7894895887169	37.0	35.0	37.0	35.0	37.0
5	35.34108990163434	37.0	35.0	37.0	33.0	37.0
6	35.43197575737037	37.0	35.0	37.0	33.0	37.0
7	35.221552895192424	37.0	35.0	37.0	32.0	37.0
8	35.747056514359464	37.0	35.0	37.0	35.0	37.0
9	37.25150457373471	39.0	37.0	39.0	34.0	39.0
10	36.86841510702172	39.0	37.0	39.0	32.0	39.0
11	36.87642020181481	39.0	37.0	39.0	33.0	39.0
12	36.53763481856154	39.0	35.0	39.0	32.0	39.0
13	36.27824941327979	39.0	35.0	39.0	32.0	39.0
14	37.39349933773338	39.0	36.0	41.0	32.0	41.0
15	37.36357166459091	39.0	36.0	40.0	32.0	41.0
16	37.38108430978827	39.0	35.0	41.0	32.0	41.0
17	37.3179289270716	39.0	35.0	40.0	32.0	41.0
18	37.22461287762612	39.0	35.0	40.0	32.0	41.0
19	37.29122644999675	39.0	35.0	40.0	32.0	41.0
20	37.164283071674475	39.0	35.0	40.0	32.0	41.0
21	37.31865614947739	39.0	35.0	40.0	32.0	41.0
22	37.34229017162449	39.0	35.0	41.0	32.0	41.0
23	37.204276067746065	39.0	35.0	41.0	32.0	41.0
24	36.802459565022154	39.0	35.0	40.0	31.0	41.0
25	36.991581165080895	39.0	35.0	40.0	32.0	41.0
26	36.98641153162923	39.0	35.0	40.0	32.0	41.0
27	36.839138403838604	39.0	35.0	40.0	31.0	41.0
28	36.875776292267155	39.0	35.0	40.0	31.0	41.0
29	36.531552979917365	38.0	35.0	40.0	31.0	41.0
30	36.62545221936982	39.0	35.0	40.0	31.0	41.0
31	36.59031113822309	39.0	35.0	40.0	31.0	41.0
32	36.45945064348592	39.0	35.0	40.0	31.0	41.0
33	36.46267301538887	39.0	35.0	40.0	31.0	41.0
34	36.32108775526918	38.0	35.0	40.0	30.0	41.0
35	36.14284060132115	38.0	35.0	40.0	30.0	41.0
36	36.115639659179806	38.0	35.0	40.0	30.0	41.0
37	35.90383436838969	38.0	35.0	40.0	30.0	41.0
38	35.81024578705234	38.0	35.0	40.0	29.0	41.0
39	35.70755633502501	38.0	35.0	40.0	29.0	41.0
40	35.55687444046237	38.0	34.0	40.0	28.0	41.0
41	35.57828866916888	38.0	34.0	40.0	28.0	41.0
42	35.52633815983078	38.0	34.0	40.0	28.0	41.0
43	35.50500159565305	38.0	34.0	40.0	28.0	41.0
44	35.39851646629218	38.0	34.0	40.0	28.0	41.0
45	35.19380830134967	37.0	34.0	40.0	27.0	41.0
46	34.208239782878216	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	9.0
11	14.0
12	18.0
13	19.0
14	34.0
15	40.0
16	90.0
17	129.0
18	301.0
19	461.0
20	852.0
21	1472.0
22	2293.0
23	3208.0
24	4136.0
25	5007.0
26	5882.0
27	6982.0
28	8323.0
29	10399.0
30	13605.0
31	17949.0
32	24342.0
33	34887.0
34	58416.0
35	68180.0
36	68412.0
37	98928.0
38	148714.0
39	125066.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.19948769652656	31.513582820041403	19.12510202294916	27.161827460482872
2	24.496522041193266	32.17005990053292	18.741298042571458	24.592120015702356
3	21.48525644827401	31.044065441544028	21.583819795699927	25.886858314482037
4	22.11532758898237	31.653943804771146	19.486312685865336	26.744415920381147
5	18.490371010514366	35.50440993315202	17.81539565135122	28.189823404982388
6	21.083660230395353	36.81439872121823	20.461214334330265	21.64072671405615
7	83.11177761397623	7.895799619867434	4.8015035852770644	4.190919180879275
8	84.38745280114775	5.750987751597771	4.177363190402359	5.68419625685213
9	80.45226173228613	8.043079808069768	6.842527401457834	4.662131058186265
10	43.168769257272935	28.31959377215204	12.742207423599286	15.769429546975744
11	32.64550802486394	28.371275985845283	20.167218790862133	18.815997198428636
12	31.252912419829027	24.78190388237919	25.280651365342415	18.68453233244937
13	26.52257778455577	30.087662071750724	23.2030546165208	20.1867055271727
14	20.532383284334077	30.756847893314355	27.738945513390778	20.97182330896079
15	20.212829050487592	28.75112613566722	29.25368624095208	21.782358572893102
16	23.59914371326821	26.86020667237148	28.458683882774572	21.08196573158574
17	22.610121241389827	27.16493404163384	25.44035787814859	24.784586838827746
18	24.204785829471287	27.122712779627605	25.36791805403757	23.304583336863537
19	25.217531284684274	29.81767192808547	24.123308678375654	20.841488108854602
20	25.58664960871198	30.34593193198282	24.602993049730713	19.46442540957448
21	24.29600634872221	28.054687124915628	26.858935798264266	20.790370728097894
22	24.23895822213185	26.88294119806714	25.557843128948537	23.320257450852473
23	23.340167811865445	29.129846619616085	23.641929808210975	23.888055760307495
24	21.904080070717082	30.462569933377953	25.677728919728764	21.955621076176193
25	23.387613778534654	27.760832789681633	25.578600739366316	23.2729526924174
26	23.72368937577488	30.43757607593614	22.707554922942666	23.131179625346313
27	21.364382199854838	29.583689884124524	25.70540573361914	23.3465221824015
28	23.557063659496112	28.576310341808654	26.311612682758756	21.555013315936478
29	22.493906864697095	30.207265446062692	25.06050772832665	22.23831996091356
30	25.37017738578372	28.685040682092257	25.136477758291043	20.808304173832983
31	28.216370552999688	28.304343282865513	22.34916842470918	21.13011773942562
32	26.445619296952444	28.73615806284896	23.299358632200562	21.518864007998033
33	26.24750414446167	27.366296983509702	24.055105101288664	22.331093770739958
34	23.249794542019334	28.211004640102573	25.06036652009252	23.47883429778557
35	23.57414985582639	30.266996529101604	25.618986294328792	20.53986732074321
36	26.73354288635279	28.088294684639653	24.380025248032265	20.798137180975296
37	23.596319548585516	31.26463270326219	22.254276491370764	22.884771256781526
38	24.71242943118499	29.276985599584282	24.213681948221765	21.796903021008962
39	22.668016617384993	27.593642240466327	25.03381937207522	24.704521770073455
40	24.5506047948668	27.483076193138974	24.287957479376537	23.67836153261769
41	21.938676088080047	25.466198984995213	27.325487803844815	25.269637123079917
42	24.59748592859947	27.31348510394338	24.277931694752986	23.811097272704167
43	24.132204797126132	25.708088690067694	25.61291434026101	24.546792172545164
44	23.738516240359008	26.41540073484765	24.529847184449018	25.31623584034432
45	24.847848127720024	27.488865730738492	24.67571529031001	22.987570851231474
46	23.120306591317952	26.103189329176164	27.64264149771101	23.13386258179487
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5761.0
1	4639.0
2	3517.0
3	2505.0
4	1493.0
5	1704.0
6	1915.0
7	1915.0
8	1924.0
9	1933.0
10	1969.5
11	2006.0
12	2145.5
13	2285.0
14	2260.0
15	2235.0
16	2139.0
17	2043.0
18	1856.5
19	1670.0
20	1670.0
21	1862.0
22	2054.0
23	2759.5
24	3465.0
25	3602.0
26	3739.0
27	4525.5
28	5312.0
29	7071.0
30	8830.0
31	8830.0
32	10103.0
33	11376.0
34	12893.0
35	14410.0
36	16652.0
37	18894.0
38	21843.0
39	24792.0
40	29784.5
41	34777.0
42	38090.5
43	41404.0
44	41404.0
45	49709.5
46	58015.0
47	61904.5
48	65794.0
49	63799.0
50	61804.0
51	59299.5
52	56795.0
53	53170.0
54	49545.0
55	46746.0
56	43947.0
57	43947.0
58	42332.5
59	40718.0
60	36644.5
61	32571.0
62	29587.0
63	26603.0
64	23407.0
65	20211.0
66	18405.0
67	16599.0
68	14653.0
69	12707.0
70	12707.0
71	10937.0
72	9167.0
73	8148.0
74	7129.0
75	6133.5
76	5138.0
77	4264.5
78	3391.0
79	2722.5
80	2054.0
81	2054.0
82	1639.5
83	1225.0
84	839.0
85	453.0
86	352.0
87	251.0
88	177.5
89	104.0
90	65.5
91	27.0
92	18.0
93	9.0
94	9.0
95	6.5
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	708174.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.78574589448069
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.50358483483409	17.95909083003729
2	9.071203981676543	4.315307057303195
3	3.197579418762267	2.281704345963016
4	1.6666116727724833	1.5856640701336675
5	1.007732494044475	1.198483451647658
6	0.6778947828846694	0.9674539829333341
7	0.5209925416584279	0.8674537346164907
8	0.4662428340260109	0.8871946860212183
9	0.3755393251430439	0.8039234665113484
>10	5.596478906185387	33.89297447403909
>50	1.795371619863336	28.224482350049346
>100	0.11239755728748159	3.6784246282880453
>500	0.004782874778190706	0.7536881172894668
>1k	0.0035871560836430293	2.5841548051668286
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4762	0.6724336109487217	No Hit
AATCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGT	3809	0.5378621638184966	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGAGATCATCGTAT	2942	0.4154346248238427	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGAGATCATCGT	2912	0.4111983777998062	No Hit
CTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTT	2642	0.3730721545834781	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1105	0.1560350987186765	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	941	0.13287694832061048	No Hit
AACTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTC	885	0.12496928720907574	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.42150657889162835	0.0
2	0.0	0.0	0.0	0.5707636823718465	0.0
3	0.0	0.0	0.0	0.8564279400260388	0.0
4	0.0	0.0	0.0	2.1275844637052477	0.0
5	0.0	0.0	0.0	2.3217457856402524	0.0
6	0.0	0.0	0.0	3.1157596861788206	0.0
7	0.0	0.0	0.0	3.7071397707343112	0.0
8	0.0	0.0	0.0	4.077670177103367	0.0
9	0.0	0.0	0.0	5.021788430526961	0.0
10	0.0	0.0	0.0	5.821309452196776	0.0
11	0.0	0.0	0.0	7.150784976573554	0.0
12	0.0	0.0	0.0	7.6246798103291	0.0
13	0.0	0.0	0.0	7.846659154388611	0.0
14	0.0	0.0	0.0	8.113119092200504	0.0
15	0.0	0.0	0.0	8.294854089531668	0.0
16	0.0	0.0	0.0	8.619774236275266	0.0
17	0.0	0.0	0.0	9.001036468438548	0.0
18	0.0	0.0	0.0	9.555278787416652	0.0
19	0.0	0.0	0.0	9.797168492489135	0.0
20	0.0	0.0	0.0	10.073089381988042	0.0
21	0.0	0.0	0.0	10.3741453371629	0.0
22	0.0	0.0	0.0	10.685509493429581	0.0
23	0.0	0.0	0.0	10.992072569735686	0.0
24	0.0	0.0	0.0	11.20543821151299	0.0
25	0.0	0.0	0.0	11.39917590874559	0.0
26	0.0	0.0	0.0	11.592489981275788	0.0
27	0.0	0.0	0.0	11.827460482875678	0.0
28	0.0	0.0	0.0	12.035036587053463	0.0
29	0.0	0.0	0.0	12.259840095795665	0.0
30	0.0	0.0	0.0	12.516839081920544	0.0
31	0.0	0.0	0.0	12.718343232030547	0.0
32	1.4120823413454886E-4	0.0	0.0	12.935945120831885	0.0
33	1.4120823413454886E-4	0.0	0.0	13.15552392491111	0.0
34	1.4120823413454886E-4	0.0	0.0	13.361264322045146	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	25	7.79162E-5	40.0	1
CGAACTC	20	0.0012554425	40.0	16
GTACCAC	20	0.0012554425	40.0	9
CGACGAA	20	0.0012554425	40.0	6
TTAGCCG	25	7.79162E-5	40.0	1
GCGAAAT	20	0.0012554425	40.0	30
ACGCAAC	20	0.0012554425	40.0	26
CTATACG	30	4.8648653E-6	40.0	1
ATGCTCG	25	7.79162E-5	40.0	1
ATTCGGA	20	0.0012554425	40.0	18
TAACGGT	20	0.0012554425	40.0	36
CGACAAT	90	0.0	40.0	20
ATGTCGC	20	0.0012554425	40.0	39
CGTTAAG	30	4.8648653E-6	40.0	1
TACGCAA	20	0.0012554425	40.0	25
GTTAACG	30	4.8648653E-6	40.0	1
CATAGCG	45	1.2205419E-9	40.0	1
CGTACTG	50	7.8216544E-11	40.0	1
AATTCGG	20	0.0012554425	40.0	2
TTACGGT	25	7.79162E-5	40.0	28
>>END_MODULE