FastQCFastQC Report
Sat 18 Jun 2016
SRR3553018_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553018_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences806683
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42280.5241216190250694No Hit
CTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTCTGCT25840.320324092611348No Hit
AATCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTCT20500.25412708585652605No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCC17650.21879722270086266No Hit
AATGATACCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGT17460.21644189849048512No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11090.13747655522677432No Hit
AAACTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTCT8960.11107213118412065No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAT207.0320343E-445.015
CGAACAG453.8380676E-1045.01
CGTATAG253.8897677E-545.01
ATCTACG453.8380676E-1045.01
CAACGGT207.0320343E-445.039
CCGTGAC207.0320343E-445.022
TCGGAAC207.0320343E-445.015
TATCTCG207.0320343E-445.01
GTTCGAC207.0320343E-445.032
CGGATAC207.0320343E-445.035
TCGCTAG207.0320343E-445.01
TTGCGAT207.0320343E-445.019
CTCGCTA207.0320343E-445.031
CTCGAAT207.0320343E-445.011
ACGTATA207.0320343E-445.033
ATCGGAC207.0320343E-445.039
ACTCACG351.2113196E-745.01
TCGCGAC207.0320343E-445.016
ACGTTGC302.1646938E-644.99999644
TAGTTGC302.1646938E-644.99999614