##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553018_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	806683
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.650369475989947	31.0	31.0	34.0	30.0	34.0
2	31.8410614826394	33.0	31.0	34.0	30.0	34.0
3	32.0512568133951	33.0	31.0	34.0	30.0	34.0
4	35.83047615978023	37.0	35.0	37.0	35.0	37.0
5	35.38495790787707	37.0	35.0	37.0	33.0	37.0
6	35.41254619224652	37.0	35.0	37.0	33.0	37.0
7	35.490292965142444	37.0	35.0	37.0	33.0	37.0
8	35.79604999733476	37.0	35.0	37.0	35.0	37.0
9	37.37156603027459	39.0	37.0	39.0	35.0	39.0
10	36.67869658837486	39.0	37.0	39.0	32.0	39.0
11	36.6675744995246	39.0	35.0	39.0	32.0	39.0
12	36.369994161275244	38.0	35.0	39.0	32.0	39.0
13	36.16459997297575	38.0	35.0	39.0	32.0	39.0
14	37.149470114034884	39.0	36.0	40.0	32.0	41.0
15	37.15221468656213	39.0	35.0	40.0	32.0	41.0
16	37.19702287019808	39.0	35.0	40.0	32.0	41.0
17	37.07939549984319	39.0	35.0	40.0	32.0	41.0
18	36.98375322152568	38.0	35.0	40.0	32.0	41.0
19	37.08315782035818	39.0	35.0	40.0	32.0	41.0
20	36.982154080351265	39.0	35.0	40.0	31.0	41.0
21	37.02272887863014	39.0	35.0	40.0	32.0	41.0
22	37.035529445891385	39.0	35.0	40.0	32.0	41.0
23	36.88136975738921	39.0	35.0	40.0	31.0	41.0
24	36.526057943454866	38.0	35.0	40.0	31.0	41.0
25	36.631738861485864	38.0	35.0	40.0	31.0	41.0
26	36.603518358512574	38.0	35.0	40.0	31.0	41.0
27	36.478764272954805	38.0	35.0	40.0	31.0	41.0
28	36.341819525141844	38.0	35.0	40.0	30.0	41.0
29	36.1867164673112	38.0	35.0	40.0	30.0	41.0
30	36.091242780621386	38.0	35.0	40.0	30.0	41.0
31	35.970770426549215	38.0	35.0	40.0	30.0	41.0
32	35.95332863094921	38.0	35.0	40.0	30.0	41.0
33	35.85651736803676	38.0	35.0	40.0	29.0	41.0
34	35.789491039231024	38.0	35.0	40.0	29.0	41.0
35	35.640573806563424	38.0	34.0	40.0	29.0	41.0
36	35.55474455269294	38.0	34.0	40.0	28.0	41.0
37	35.41734237612544	38.0	34.0	40.0	28.0	41.0
38	35.360582781588306	38.0	34.0	40.0	27.0	41.0
39	35.283029393206505	38.0	34.0	40.0	27.0	41.0
40	35.12408963620158	38.0	34.0	40.0	26.0	41.0
41	35.098495939545025	38.0	34.0	40.0	27.0	41.0
42	35.02222310374707	37.0	34.0	40.0	26.0	41.0
43	35.001004111900215	37.0	34.0	40.0	26.0	41.0
44	34.85662273780407	37.0	34.0	40.0	26.0	41.0
45	34.65155953453835	37.0	33.0	40.0	25.0	41.0
46	34.620110997752526	37.0	33.0	40.0	25.0	41.0
47	34.53581518390743	37.0	33.0	40.0	25.0	41.0
48	34.50366996701307	37.0	33.0	40.0	25.0	41.0
49	34.3255070455185	36.0	33.0	40.0	24.0	41.0
50	34.19486836836775	36.0	33.0	40.0	24.0	41.0
51	32.63706189420132	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	8.0
11	13.0
12	22.0
13	39.0
14	58.0
15	84.0
16	179.0
17	315.0
18	576.0
19	1141.0
20	1978.0
21	3074.0
22	4177.0
23	5166.0
24	6007.0
25	6694.0
26	7786.0
27	9357.0
28	10981.0
29	13967.0
30	17971.0
31	23700.0
32	31684.0
33	44569.0
34	71315.0
35	79147.0
36	84985.0
37	115135.0
38	149672.0
39	116866.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.109903146589183	33.15143618992838	20.78437254782858	24.954288115653856
2	23.27047923409815	31.942906941140446	19.076266637576346	25.71034718718505
3	21.06304459124588	32.41074870798071	20.304382266640054	26.221824434133357
4	20.197400961716063	32.32719668072836	19.237792292635397	28.237610064920172
5	17.582619194900598	35.66270765591936	17.663196075781936	29.091477073398103
6	20.41582629112055	37.80357339872044	18.720612681809335	23.059987628349674
7	83.93309391669342	7.401668313327541	4.791969088229205	3.873268681749832
8	85.73875983502813	6.388011151840314	3.703437409738398	4.169791603393155
9	80.05759387516534	9.202995476537872	6.5437104785894835	4.195700169707307
10	39.69006412680074	32.45029336182863	11.99045969730365	15.869182814066987
11	31.452999505381918	28.13075272442831	22.73011827446469	17.686129495725087
12	30.45582961336733	25.699686246022292	25.63187770164984	18.21260643896053
13	25.964474273041578	30.257238593102866	24.008811391835454	19.7694757420201
14	20.96238547236027	31.958030601859715	26.493678433783778	20.585905491996236
15	18.936186829274945	30.946232906854366	29.652416128764337	20.465164135106356
16	21.901168116843916	27.791957931430318	28.516282108337478	21.790591843388295
17	21.34481574546631	28.370128042861943	25.73662764679558	24.548428564876165
18	23.041392963530903	28.971603467533097	26.033894355031656	21.953109213904344
19	23.35663451442512	32.000798330942885	23.746254724594422	20.89631243003757
20	24.267897054976988	29.858321050524182	25.04366647121608	20.830115423282752
21	23.075979040093817	29.938030180380643	25.586630683924167	21.39936009560137
22	23.129779603636123	27.880592500399786	25.47221151307267	23.51741638289142
23	21.99438936980202	30.255503091053114	23.768196429080568	23.9819111100643
24	20.28739913943891	30.951067519707248	27.068253576683777	21.693279764170065
25	21.72774187630085	30.919580553947462	24.331614773089306	23.02106279666238
26	22.249384206683416	31.407876452088367	23.564150973802597	22.778588367425616
27	19.5874959556604	30.774418203928928	25.0201132291123	24.617972611298367
28	20.0895519057672	31.199988099414515	26.729954641414285	21.980505353404002
29	20.85664381175753	29.095691864090355	26.384217840217282	23.66344648393483
30	22.560906824613884	30.415045315198164	24.61809657573049	22.405951284457462
31	23.5661344047166	31.636466864927115	22.6438390297056	22.15355970065069
32	24.195377862183783	28.33727746834878	23.300974484400935	24.1663701850665
33	23.7727831130692	29.958856204977668	22.510949158467454	23.75741152348568
34	19.707369561525407	29.92265859079713	25.385684339449327	24.98428750822814
35	21.36365833914933	29.81840450337989	25.300644739011485	23.517292418459295
36	23.82348456580838	29.705596870146017	24.577560206425574	21.893358357620034
37	20.777802432925945	30.89268027217631	23.74104821844516	24.588469076452586
38	22.53983287115261	29.78728943091648	24.787679918877675	22.885197779053236
39	22.318060502080744	27.888278295191544	24.903090805186174	24.890570397541538
40	24.40413396588251	28.546157536479633	23.298619160190558	23.7510893374473
41	20.899535505272826	26.6279319137753	27.39713121511176	25.07540136584011
42	22.823463491854916	28.186412754452494	24.403142250425507	24.586981503267083
43	21.900176401386915	27.1823008542389	26.231121766542742	24.68640097783144
44	22.522849743951465	26.845861385451286	24.764250641206026	25.86703822939122
45	22.186038381867473	29.784190320113353	24.33632542151006	23.693445876509113
46	21.828277030754336	27.999598355239915	27.13668194321685	23.035442670788896
47	21.851830272858113	28.169925484979853	25.71369422685243	24.26455001530961
48	21.359691477321327	26.979371078850058	28.500662589889707	23.16027485393891
49	22.307523525350106	26.45921632165299	26.938958673977265	24.29430147901964
50	21.197917893398028	27.242423603819592	26.795159932712103	24.764498570070277
51	21.311221384360397	26.940694176027012	27.02846099397161	24.719623445640977
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5233.0
1	4233.0
2	3233.0
3	2326.0
4	1419.0
5	1448.5
6	1478.0
7	1625.5
8	1773.0
9	2003.5
10	2234.0
11	2518.5
12	2803.0
13	2898.5
14	2994.0
15	2968.5
16	2943.0
17	2765.5
18	2588.0
19	2520.0
20	2452.0
21	2723.0
22	2994.0
23	3161.0
24	3328.0
25	3393.5
26	4480.0
27	5501.0
28	7582.5
29	9664.0
30	11332.5
31	13001.0
32	15666.5
33	18332.0
34	20520.5
35	22709.0
36	25246.0
37	27783.0
38	29119.0
39	30455.0
40	33456.0
41	36457.0
42	41133.0
43	45809.0
44	53439.0
45	61069.0
46	62364.0
47	63659.0
48	67592.0
49	71525.0
50	67948.5
51	64372.0
52	59096.0
53	53820.0
54	48558.0
55	43296.0
56	40299.5
57	37303.0
58	34520.5
59	31738.0
60	29420.5
61	27103.0
62	25296.5
63	23490.0
64	22257.0
65	21024.0
66	17650.5
67	14277.0
68	12812.5
69	11348.0
70	10335.0
71	9322.0
72	8565.0
73	7808.0
74	7374.5
75	5473.0
76	4005.0
77	3179.5
78	2354.0
79	2166.5
80	1979.0
81	1365.5
82	752.0
83	559.0
84	366.0
85	288.0
86	210.0
87	157.5
88	105.0
89	83.5
90	62.0
91	63.5
92	65.0
93	44.0
94	23.0
95	18.5
96	14.0
97	12.0
98	10.0
99	5.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	806683.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.088038338795673
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.53103963129377	22.05782851949262
2	8.835129141531208	4.963228921110789
3	3.1322152357548627	2.639333448817276
4	1.5427158995330525	1.7332745332781614
5	0.9371844510142301	1.3161836395305435
6	0.6495967887581227	1.0947539704438023
7	0.47314928255233496	0.9302884631812556
8	0.3654727785269064	0.8212330732039943
9	0.2842173000604102	0.7184795778581216
>10	3.5847509670909203	24.218884934272875
>50	1.3982571257368042	27.713394426939285
>100	0.2600687366264465	9.470009306139405
>500	0.0035443780119447555	0.6453628597174594
>1k	0.0026582835089585667	1.6777443260144211
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4228	0.5241216190250694	No Hit
CTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTCTGCT	2584	0.320324092611348	No Hit
AATCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTCT	2050	0.25412708585652605	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCC	1765	0.21879722270086266	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGT	1746	0.21644189849048512	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1109	0.13747655522677432	No Hit
AAACTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTCT	896	0.11107213118412065	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.958577285005386E-4	0.0	0.0	0.3891243524407977	0.0
2	4.958577285005386E-4	0.0	0.0	0.5217662948146917	0.0
3	4.958577285005386E-4	0.0	0.0	0.7654803683727065	0.0
4	4.958577285005386E-4	0.0	0.0	1.9295063860277208	0.0
5	4.958577285005386E-4	0.0	0.0	2.1033045198671596	0.0
6	4.958577285005386E-4	0.0	0.0	3.062913188947827	0.0
7	4.958577285005386E-4	0.0	0.0	3.739511059486812	0.0
8	4.958577285005386E-4	0.0	0.0	4.2647483584010075	0.0
9	4.958577285005386E-4	0.0	0.0	5.144028075464588	0.0
10	4.958577285005386E-4	0.0	0.0	6.2138411247045	0.0
11	4.958577285005386E-4	0.0	0.0	7.417039902911057	0.0
12	4.958577285005386E-4	0.0	0.0	7.970789021214033	0.0
13	4.958577285005386E-4	0.0	0.0	8.22082528081043	0.0
14	4.958577285005386E-4	0.0	0.0	8.453754448773559	0.0
15	4.958577285005386E-4	0.0	0.0	8.642552278900139	0.0
16	4.958577285005386E-4	0.0	0.0	9.010602677879662	0.0
17	4.958577285005386E-4	0.0	0.0	9.447453336688637	0.0
18	4.958577285005386E-4	0.0	0.0	10.078804189501948	0.0
19	4.958577285005386E-4	0.0	0.0	10.332311453197848	0.0
20	4.958577285005386E-4	0.0	0.0	10.652511581377071	0.0
21	4.958577285005386E-4	0.0	0.0	10.990934481078689	0.0
22	4.958577285005386E-4	0.0	0.0	11.33667128227569	0.0
23	4.958577285005386E-4	0.0	0.0	11.702490321476962	0.0
24	4.958577285005386E-4	0.0	0.0	11.967278348496249	0.0
25	4.958577285005386E-4	0.0	0.0	12.19376136598887	0.0
26	4.958577285005386E-4	0.0	0.0	12.407352082540477	0.0
27	4.958577285005386E-4	0.0	0.0	12.68986702335366	0.0
28	4.958577285005386E-4	0.0	0.0	12.92118465369916	0.0
29	4.958577285005386E-4	0.0	0.0	13.213492784650228	0.0
30	4.958577285005386E-4	0.0	0.0	13.556874261636851	0.0
31	4.958577285005386E-4	0.0	0.0	13.796497508934737	0.0
32	4.958577285005386E-4	0.0	0.0	14.03822815157875	0.0
33	4.958577285005386E-4	0.0	0.0	14.285289264804142	0.0
34	4.958577285005386E-4	0.0	0.0	14.554416046947809	0.0
35	4.958577285005386E-4	0.0	0.0	14.847096071195253	0.0
36	4.958577285005386E-4	0.0	0.0	15.106305698768908	0.0
37	4.958577285005386E-4	0.0	0.0	15.340102617756914	0.0
38	4.958577285005386E-4	0.0	0.0	15.634022286325607	0.0
39	4.958577285005386E-4	0.0	0.0	16.052526209180062	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAT	20	7.0320343E-4	45.0	15
CGAACAG	45	3.8380676E-10	45.0	1
CGTATAG	25	3.8897677E-5	45.0	1
ATCTACG	45	3.8380676E-10	45.0	1
CAACGGT	20	7.0320343E-4	45.0	39
CCGTGAC	20	7.0320343E-4	45.0	22
TCGGAAC	20	7.0320343E-4	45.0	15
TATCTCG	20	7.0320343E-4	45.0	1
GTTCGAC	20	7.0320343E-4	45.0	32
CGGATAC	20	7.0320343E-4	45.0	35
TCGCTAG	20	7.0320343E-4	45.0	1
TTGCGAT	20	7.0320343E-4	45.0	19
CTCGCTA	20	7.0320343E-4	45.0	31
CTCGAAT	20	7.0320343E-4	45.0	11
ACGTATA	20	7.0320343E-4	45.0	33
ATCGGAC	20	7.0320343E-4	45.0	39
ACTCACG	35	1.2113196E-7	45.0	1
TCGCGAC	20	7.0320343E-4	45.0	16
ACGTTGC	30	2.1646938E-6	44.999996	44
TAGTTGC	30	2.1646938E-6	44.999996	14
>>END_MODULE