FastQCFastQC Report
Sat 18 Jun 2016
SRR3553016_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553016_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences588685
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGT185823.1565268352344633No Hit
AATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT164772.798950202570135No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCC146792.493523701130486No Hit
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT68281.1598732768798252TruSeq Adapter, Index 16 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47670.8097709301239202No Hit
AATGACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTT42340.7192301485514324No Hit
AATGATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCT32140.5459626115834445No Hit
AACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG30300.5147065068754937No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTAT20130.3419485803103527No Hit
AAACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT17410.29574390378555593No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11840.20112623899029192No Hit
AATGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC11650.19789870643892746No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTT10550.17921299166786991No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9190.15611065340547153No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCCTGACCTTTCGTATGCCGT7700.1308000033974027No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGACCTTTC6480.11007584701495707No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGT207.030479E-445.036
TCCGCGA207.030479E-445.02
CGAGTTA253.888479E-545.017
ATAATCG207.030479E-445.016
CGTTGCC253.888479E-545.017
ATAGCTA253.888479E-545.030
TAAACGG551.8189894E-1245.02
TAAACCG551.8189894E-1245.01
TGTATCG207.030479E-445.021
CGACAGA207.030479E-445.023
AAGCGAA207.030479E-445.020
TACGGAA207.030479E-445.04
CGCGAAC207.030479E-445.023
ATCACCG406.8048394E-945.01
TAGCCGT453.8380676E-1045.044
TAGCCCG253.888479E-545.022
TCGGCGC207.030479E-445.026
CGTTAGC253.888479E-545.040
CGTTAAG253.888479E-545.01
CCGTATA207.030479E-445.038