##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553007_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	884980
Sequences flagged as poor quality	0
Sequence length	46
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.864839883387194	33.0	31.0	34.0	30.0	34.0
2	32.08965061357319	33.0	31.0	34.0	30.0	34.0
3	32.27935320572216	34.0	31.0	34.0	30.0	34.0
4	35.976813035322834	37.0	35.0	37.0	35.0	37.0
5	35.676757666839926	37.0	35.0	37.0	35.0	37.0
6	35.746023638952295	37.0	35.0	37.0	35.0	37.0
7	35.59757282650455	37.0	35.0	37.0	33.0	37.0
8	35.91617550679111	37.0	35.0	37.0	35.0	37.0
9	37.36184320549617	39.0	37.0	39.0	34.0	39.0
10	36.94153314199191	39.0	37.0	39.0	33.0	39.0
11	37.096108386630206	39.0	37.0	39.0	34.0	39.0
12	36.850901715293	39.0	35.0	39.0	33.0	39.0
13	36.68208773079618	39.0	35.0	39.0	32.0	39.0
14	37.914584510384415	40.0	37.0	41.0	33.0	41.0
15	37.819586883319396	40.0	37.0	41.0	33.0	41.0
16	37.73358832967977	39.0	36.0	41.0	33.0	41.0
17	37.589865307690566	39.0	36.0	40.0	33.0	41.0
18	37.2568091934281	38.0	36.0	40.0	33.0	41.0
19	37.11711112115528	38.0	35.0	40.0	33.0	41.0
20	36.949012407060046	38.0	35.0	40.0	32.0	41.0
21	36.97784356708626	38.0	35.0	40.0	33.0	41.0
22	37.009455580917084	38.0	35.0	40.0	33.0	41.0
23	36.843977265022936	38.0	35.0	40.0	33.0	41.0
24	36.57874076250311	38.0	35.0	40.0	32.0	41.0
25	36.64923953083686	38.0	35.0	40.0	32.0	41.0
26	36.66597889217835	38.0	35.0	40.0	32.0	41.0
27	36.52542430337409	38.0	35.0	40.0	32.0	41.0
28	36.501830549842936	38.0	35.0	40.0	32.0	41.0
29	36.25835047119709	38.0	35.0	40.0	31.0	41.0
30	36.28680083165721	38.0	35.0	40.0	31.0	41.0
31	36.22238016678343	38.0	35.0	40.0	31.0	41.0
32	36.14566430879794	38.0	35.0	40.0	31.0	41.0
33	36.10779000655382	38.0	35.0	40.0	31.0	41.0
34	35.93123799407896	38.0	35.0	40.0	31.0	41.0
35	35.69438066396981	38.0	35.0	40.0	30.0	41.0
36	35.56726931682072	38.0	35.0	40.0	30.0	41.0
37	35.41313137020045	38.0	35.0	40.0	29.0	41.0
38	35.104272412935885	37.0	35.0	40.0	26.0	41.0
39	34.917241067594745	37.0	35.0	40.0	25.0	41.0
40	34.57939388460756	37.0	34.0	40.0	23.0	41.0
41	34.44395918551832	37.0	34.0	40.0	22.0	41.0
42	34.2368878392732	37.0	34.0	40.0	21.0	41.0
43	34.00406562860178	36.0	33.0	40.0	18.0	41.0
44	33.78729462812719	36.0	33.0	40.0	18.0	41.0
45	33.26958010350516	35.0	33.0	40.0	15.0	41.0
46	32.534217722434406	35.0	32.0	40.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	10.0
11	18.0
12	18.0
13	23.0
14	28.0
15	56.0
16	94.0
17	165.0
18	293.0
19	541.0
20	1037.0
21	1794.0
22	2909.0
23	4238.0
24	5803.0
25	7390.0
26	9020.0
27	10541.0
28	12225.0
29	14807.0
30	18794.0
31	24881.0
32	32637.0
33	45474.0
34	74024.0
35	119235.0
36	95083.0
37	93666.0
38	155251.0
39	154917.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.324188117245587	37.68977830007458	18.25815272661529	23.727880856064544
2	19.217270446789758	42.130443625844656	18.105380912562996	20.546905014802594
3	17.870686343194198	43.486406472462654	17.93577255983186	20.70713462451129
4	16.377206264548352	42.76955411421727	18.20436620036611	22.648873420868266
5	15.027684241451784	45.494813442111685	16.08025040113901	23.39725191529752
6	16.381613143799857	48.45465434247102	17.307961761847725	17.855770751881398
7	69.50936744333205	21.876426585911545	5.140229157721079	3.4739768130353226
8	71.51754841917331	20.951433930710298	4.2929783723925965	3.2380392777237907
9	66.37573730479784	23.78370132658365	6.427602883680987	3.412958484937513
10	30.757418246740038	47.33112612714412	10.550972903342448	11.360482722773396
11	23.3490022373387	41.69766548396574	22.82492259712084	12.128409681574725
12	23.911726818685167	38.51612465818437	24.614341567041063	12.957806956089405
13	18.52516441049515	42.76232231236864	23.418834323939524	15.293678953196682
14	13.646410088363577	43.99839544396483	27.006712016090763	15.348482451580828
15	12.056317656896201	44.91480033447083	29.42959162918936	13.599290379443604
16	14.265520124748582	43.62448868900992	27.62932495649619	14.480666229745303
17	14.509819430947593	42.41734276480825	25.776514723496575	17.296323080747587
18	15.891997559266876	43.023571154150375	25.731089968134874	15.353341318447875
19	16.46206693936586	44.10868042215643	25.129720445659785	14.299532192817917
20	16.90829171280707	42.89068679518181	26.646251892698142	13.55476959931298
21	16.739587335307014	44.7906167371014	24.336143189676605	14.133652737914982
22	16.18646749079075	43.08425049153653	24.57874754231734	16.150534475355375
23	14.458066848968338	44.00867816221836	24.6825916969875	16.850663291825803
24	13.120070510068023	42.770684083256114	28.61951682523899	15.489728581436868
25	14.17851250875726	44.045176162173156	26.336753372957585	15.439557956112
26	15.420009491739926	45.547582996225906	23.094533209790054	15.937874302244118
27	12.374064950620353	46.62659043142218	24.28416461388958	16.71518000406789
28	14.586883319397048	42.71350764989039	29.02935659562928	13.67025243508328
29	13.95104974123709	42.05812560735836	27.312368641099233	16.678456010305318
30	15.173337250559335	43.941896992022414	26.86716083979299	14.017604917625256
31	18.10854482587177	43.461095165992454	24.06189970394811	14.368460304187666
32	17.364460213790142	42.04535695721937	24.37885601934507	16.211326809645417
33	18.817939388460754	41.74060430744198	24.828583696806707	14.612872607290562
34	15.831205224976836	41.61088386178219	26.833148771723653	15.724762141517324
35	15.360573120296502	40.011186693484596	26.920834369138287	17.707405817080613
36	17.503898393184027	41.58229564509933	27.303554882596217	13.610251079120433
37	16.36590657415987	42.218694207778704	26.853375217519037	14.562024000542383
38	16.71687495762616	40.826120364302014	25.969400438427986	16.487604239643833
39	16.851793260864653	40.13842120725892	27.043323012949443	15.966462518926983
40	18.465389048340075	40.30350968383466	24.46089177156546	16.770209496259803
41	15.993242785147688	39.552419263712174	26.844674455919908	17.609663495220232
42	17.553617030893353	38.62301972925942	25.983073063798052	17.840290176049177
43	15.887138692399827	39.495355827250336	26.449072295419107	18.168433184930734
44	16.6785690072092	38.450699450835046	26.00499446315171	18.86573707880404
45	16.86975976858234	40.68713417252367	24.703157133494543	17.739948925399442
46	17.073832176998348	39.72993739971525	26.44523040068702	16.75100002259938
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	85024.0
1	63653.0
2	42282.0
3	25838.5
4	9395.0
5	7821.0
6	6247.0
7	6247.0
8	6174.0
9	6101.0
10	6578.5
11	7056.0
12	7161.0
13	7266.0
14	7922.0
15	8578.0
16	8556.0
17	8534.0
18	8154.5
19	7775.0
20	7775.0
21	10204.5
22	12634.0
23	12851.0
24	13068.0
25	12440.5
26	11813.0
27	16286.5
28	20760.0
29	25575.0
30	30390.0
31	30390.0
32	34907.5
33	39425.0
34	42385.5
35	45346.0
36	45170.5
37	44995.0
38	42484.0
39	39973.0
40	42720.0
41	45467.0
42	48417.5
43	51368.0
44	51368.0
45	50793.5
46	50219.0
47	52807.5
48	55396.0
49	54507.0
50	53618.0
51	50600.0
52	47582.0
53	40619.0
54	33656.0
55	28825.0
56	23994.0
57	23994.0
58	21480.5
59	18967.0
60	17764.0
61	16561.0
62	15396.0
63	14231.0
64	12434.5
65	10638.0
66	8383.5
67	6129.0
68	4894.5
69	3660.0
70	3660.0
71	3545.5
72	3431.0
73	2532.0
74	1633.0
75	1170.0
76	707.0
77	561.5
78	416.0
79	417.0
80	418.0
81	418.0
82	245.0
83	72.0
84	53.0
85	34.0
86	43.5
87	53.0
88	33.5
89	14.0
90	14.5
91	15.0
92	16.5
93	18.0
94	18.0
95	17.0
96	16.0
97	10.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	884980.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.99888658022591
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.12286347060508	15.423713992908791
2	10.064657166135552	4.025638742688057
3	3.3235102892033574	1.993995159659753
4	1.6184323403318421	1.2946737924826437
5	0.9271832366150845	0.927131619407592
6	0.6342187212496654	0.7610200963996783
7	0.490125062959407	0.6861368880975884
8	0.36168403661870757	0.5786622420972646
9	0.3215179442137001	0.5787000809853472
>10	3.559177930954549	16.506505327847098
>50	1.0834363896057824	15.249099933825894
>100	0.41260254334384683	16.65743207857295
>500	0.06526725238589048	8.989127111514938
>1k	0.013053450477178099	3.3118732794627936
>5k	5.675413250946998E-4	1.0007466570144095
>10k+	0.0017026239752840997	12.015542997035206
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	73724	8.33058374200547	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18264	2.0637754525526	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13874	1.567719044498181	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8817	0.9962937015525775	No Hit
GCACAAGGGGGATTTTTTTAAAATAAGTAAATTTCTTATTGACGGC	1823	0.20599335578205158	No Hit
TTCACTGGGAACAATAATTTTTTTTAATCTAAGTATAGGTAATATA	1718	0.19412868087414406	No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1613	0.18226400596623651	No Hit
TGCTTTATTTCCTGAAGGCATATTTAGATCAAATCTATCAATCTCT	1567	0.17706614838753418	No Hit
GAAGATGGGAGTTTTAATTTTCTCTTATTGACTTTTGCATCTTTAG	1504	0.16994734344278967	No Hit
CATAGCGGCAAGATTTTTTTATTTCCCTCATTTCCTTCAATACTTC	1370	0.15480575832222196	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1350	0.1525458202445253	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1345	0.15198083572510115	No Hit
ATCGAGGGGGGATTGATTGTTTTTCGTGTAATGAATTCTGTTAACT	1308	0.1477999502813623	No Hit
AGACGAGGACGTGAAGGATAACTGGGATGATGATGATGATGAAAAT	1244	0.14056814843273296	No Hit
ATTCTGGGGATTTTTAGTTTTTATGGGGATTTTTAGATGTGAAGAA	1172	0.13243237135302494	No Hit
CAGCTAGGGATACAAATGTTAGCTTTTGAAAGTAATTGAAATAATG	1154	0.13039842708309793	No Hit
TAGGGGGAGCGGGTCTACATGAATTATTTGCGATTGGTAATTATGT	1134	0.12813848900540126	No Hit
AGCTCTGGGCTGTCTGTTATAAACGATTTTATTATAGTTGTTTATT	1129	0.1275735044859771	No Hit
ATACAGGGAAAATGTGGGCCAAAAATTTGTTGCATCTTTTCATATA	1114	0.12587855092770459	No Hit
CATCATGGGCTACTATTTAAATATTTTCATTTTATTGTGACTTTGT	1111	0.1255395602160501	No Hit
AGAAACGGAAGAAAGGAAAGGAAAGAAAGAAATAAAAGAAAGAAAG	1105	0.12486157879274108	No Hit
TTGTGAGGGAAAAATTTAATGATTGTATATGAAGATTTTAATGACT	1090	0.12316662523446857	No Hit
TTTGTAGGGACTGTATTATTTGTATATAAATATGAACTATAGTTTT	1090	0.12316662523446857	No Hit
GGCTTGCAGAATTCTTTCTTACTTAATTTTCATTGTAAATTTCCCC	1073	0.1212456778684264	No Hit
GTGCAAAGGGATCTTCAATTTCTTAACTTTGGTATTAATAAACTTT	1069	0.12079369025288708	No Hit
ATAACCGGGAATGTAACATTGAGTTAGTTTGTGTTACTCTGTGTTT	1068	0.12068069334900224	No Hit
GCACTACTTCTGATTTTTGCAGTGTTCAGTCCTAGTTTTTCTTTAT	1028	0.1161608171936089	No Hit
ACGTATGGGACAGATAGTTTTTTTGTTTACGATTTATGACAGGTTT	989	0.11175393794210038	No Hit
GCAGTATGGGATTTCTAAAAATAATGTTAAGAATTTTTGCTGGTTT	978	0.11051097199936723	No Hit
CGAGCAGGAGCACTTTACTAAAGTCTCTTTACATTCTCATATAGAA	973	0.10994598747994305	No Hit
TATCTTGGGGAGATTCCTGAATTCATTCGGTTCATTGATGTTATAA	949	0.10723406178670704	No Hit
ATCAACGGGAAAATAAATAAATAAATAAATCTTTTTTTAAAATTAC	921	0.1040701484779317	No Hit
CAGCGAAGGGATGGATTTTGTTAAACAAATTTAAGGGATGATCAAT	919	0.10384415467016203	No Hit
AGATATGGGAGCAGGTGGATTATTGTCAGTGTGTTTATATGTATAT	912	0.1030531763429682	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	906	0.1023751949196592	No Hit
GATGAGGGGGAGAAGAAGGAAGAAGAGGAGAAGAAGGAAGAAGAGG	902	0.10192320730411987	No Hit
GTAAACGGGAGATAATGTTGGATATATTTAGATTTTGCCAAGGGCT	894	0.10101923207304118	No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	891	0.10068024136138669	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5054351510768605	0.0
2	0.0	0.0	0.0	0.7174173427648083	0.0
3	0.0	0.0	0.0	0.9547108409229587	0.0
4	0.0	0.0	0.0	2.075414133652738	0.0
5	0.0	0.0	0.0	2.2479604058848786	0.0
6	0.0	0.0	0.0	3.066622974530498	0.0
7	0.0	0.0	0.0	3.6455061131325	0.0
8	0.0	0.0	0.0	3.925964428574657	0.0
9	0.0	0.0	0.0	4.588691269859206	0.0
10	0.0	0.0	0.0	5.435829058283803	0.0
11	0.0	0.0	0.0	6.493932066261385	0.0
12	0.0	0.0	0.0	6.997446269972203	0.0
13	0.0	0.0	0.0	7.184230152093833	0.0
14	0.0	0.0	0.0	7.384347668873873	0.0
15	0.0	0.0	0.0	7.55282605256616	0.0
16	0.0	0.0	0.0	7.89204275802843	0.0
17	0.0	0.0	0.0	8.308436348844042	0.0
18	0.0	0.0	0.0	8.81873036678795	0.0
19	0.0	0.0	0.0	9.076702298357025	0.0
20	0.0	0.0	0.0	9.360324527107958	0.0
21	0.0	0.0	0.0	9.672421975637867	0.0
22	0.0	0.0	0.0	10.014463603697259	0.0
23	0.0	0.0	0.0	10.355375262717802	0.0
24	0.0	0.0	0.0	10.586227937354517	0.0
25	0.0	0.0	0.0	10.78126059345974	0.0
26	0.0	0.0	0.0	10.957422766616194	0.0
27	0.0	0.0	0.0	11.201496079007436	0.0
28	0.0	0.0	0.0	11.413139279983728	0.0
29	0.0	0.0	0.0	11.631223304481457	0.0
30	0.0	0.0	0.0	11.900268932631246	0.0
31	1.1299690388483356E-4	0.0	0.0	12.112025130511425	0.0
32	1.1299690388483356E-4	0.0	0.0	12.304119867115642	0.0
33	1.1299690388483356E-4	0.0	0.0	12.49508463468101	0.0
34	1.1299690388483356E-4	0.0	0.0	12.695993129788244	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGTAA	55	5.456968E-12	40.000004	13
ACTAGCT	55	5.456968E-12	40.000004	40
TCGTAAA	55	5.456968E-12	40.000004	14
TCACGCG	35	3.05532E-7	40.0	1
CGGAATA	80	0.0	40.0	19
AAGTACG	25	7.7931996E-5	40.0	1
TCGTTGA	40	1.9274012E-8	40.0	24
CGAACTG	40	1.9274012E-8	40.0	1
AAATCCG	40	1.9274012E-8	40.0	1
CGTATCG	20	0.0012556121	40.0	1
ATTAGCG	90	0.0	40.0	1
AATCACG	20	0.0012556121	40.0	1
GTCGAGC	20	0.0012556121	40.0	7
GGTCGAG	20	0.0012556121	40.0	6
GCGAATC	25	7.7931996E-5	40.0	28
TCCGATC	45	1.2205419E-9	40.0	10
TTTCGCC	20	0.0012556121	40.0	29
CATGAGT	20	0.0012556121	40.0	16
CGTTGAT	40	1.9274012E-8	40.0	25
GGCAACG	50	7.8216544E-11	40.0	1
>>END_MODULE