FastQCFastQC Report
Sat 18 Jun 2016
SRR3552996_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552996_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences648096
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCC43250.6673394065076779No Hit
CTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGCT42950.6627104626475091No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGT35180.5428208166691355No Hit
AATCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCT32830.5065607564311461No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28360.4375894929146299No Hit
AAACTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCT12880.19873598972991657No Hit
AAAAACTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTT11480.1771342517157952No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG7890.12174122352244111No Hit
AATGACTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTT7620.11757517404828914No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7010.10816298819927912No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAT207.031006E-445.00000418
CGAACCG207.031006E-445.0000041
CAACGGA207.031006E-445.0000043
TTAGCTT302.1640299E-645.00000444
CGAAACG453.8380676E-1045.0000041
ACGCATG302.1640299E-645.0000041
CGTGCGT207.031006E-445.00000417
CCGATAT207.031006E-445.00000445
TAGACGT207.031006E-445.00000426
TCCGAAC207.031006E-445.00000435
TCACACG207.031006E-445.0000041
GGGTATA302.1640299E-645.0000047
CGACCGA302.1640299E-645.00000428
TCGTGGG800.045.0000043
TAACCGC207.031006E-445.00000424
GCCGAAC207.031006E-445.00000422
CGCTCAC207.031006E-445.00000425
CTACGAC207.031006E-445.00000429
TACGACA207.031006E-445.00000430
TGCGACC207.031006E-445.00000418