##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552996_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	648096
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.196404545005677	31.0	31.0	34.0	28.0	34.0
2	31.41380752234237	31.0	31.0	34.0	28.0	34.0
3	31.40183244457611	31.0	31.0	34.0	28.0	34.0
4	35.415771737520366	37.0	35.0	37.0	33.0	37.0
5	35.069881622475684	37.0	35.0	37.0	32.0	37.0
6	35.14007955611515	37.0	35.0	37.0	32.0	37.0
7	35.06039537352491	37.0	35.0	37.0	32.0	37.0
8	35.44670542635659	37.0	35.0	37.0	33.0	37.0
9	35.888957808719695	39.0	37.0	39.0	32.0	39.0
10	35.76567977583568	38.0	35.0	39.0	30.0	39.0
11	36.140281686663705	38.0	35.0	39.0	32.0	39.0
12	35.73910809509702	37.0	35.0	39.0	31.0	39.0
13	35.425183923369374	37.0	35.0	39.0	30.0	39.0
14	36.49911587172271	38.0	35.0	40.0	31.0	41.0
15	36.61112859823236	38.0	35.0	40.0	31.0	41.0
16	36.641946563472075	38.0	35.0	40.0	31.0	41.0
17	36.58239674369229	38.0	35.0	40.0	31.0	41.0
18	36.45520262430257	38.0	35.0	40.0	31.0	41.0
19	36.48895379696835	38.0	35.0	40.0	31.0	41.0
20	36.400385128129166	38.0	35.0	40.0	31.0	41.0
21	36.41438922628746	38.0	35.0	40.0	31.0	41.0
22	36.396435096035155	38.0	35.0	40.0	31.0	41.0
23	36.33470041475337	38.0	35.0	40.0	31.0	41.0
24	35.953281612600605	38.0	34.0	40.0	30.0	41.0
25	36.07955457216215	38.0	34.0	40.0	30.0	41.0
26	35.90812317928208	38.0	34.0	40.0	30.0	41.0
27	35.83161136621735	38.0	34.0	40.0	30.0	41.0
28	35.77452723053375	38.0	34.0	40.0	29.0	41.0
29	35.485534550436974	37.0	34.0	40.0	29.0	41.0
30	35.605313101762704	38.0	34.0	40.0	29.0	41.0
31	35.40872185602133	37.0	34.0	40.0	29.0	41.0
32	35.303147990421174	37.0	34.0	40.0	28.0	41.0
33	35.28357527279909	37.0	34.0	40.0	28.0	41.0
34	35.092288796721476	37.0	34.0	40.0	27.0	41.0
35	35.036235372537405	37.0	34.0	40.0	27.0	41.0
36	34.789685478694516	37.0	33.0	40.0	26.0	41.0
37	34.638925406112676	37.0	33.0	40.0	25.0	41.0
38	34.608877388535035	37.0	33.0	40.0	26.0	41.0
39	34.428147990421174	36.0	33.0	40.0	25.0	41.0
40	34.273436342764036	36.0	33.0	40.0	24.0	41.0
41	34.248495593245444	36.0	33.0	40.0	24.0	41.0
42	34.27290864316397	36.0	33.0	40.0	24.0	41.0
43	34.20600497457167	36.0	33.0	40.0	24.0	41.0
44	34.15082024885202	36.0	33.0	40.0	24.0	41.0
45	33.91009973831037	35.0	33.0	39.0	23.0	41.0
46	33.70769145311806	35.0	33.0	39.0	23.0	41.0
47	33.57019793363946	35.0	33.0	39.0	23.0	40.0
48	33.459921061077374	35.0	32.0	39.0	23.0	40.0
49	33.33752561348936	35.0	32.0	39.0	22.0	40.0
50	33.21730268355305	35.0	32.0	39.0	22.0	40.0
51	31.35936651360292	34.0	29.0	38.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	15.0
10	21.0
11	20.0
12	16.0
13	27.0
14	31.0
15	72.0
16	165.0
17	330.0
18	595.0
19	1086.0
20	1801.0
21	2581.0
22	3525.0
23	4738.0
24	5886.0
25	6788.0
26	8430.0
27	9934.0
28	12104.0
29	15213.0
30	19451.0
31	24430.0
32	32290.0
33	43508.0
34	70524.0
35	72737.0
36	67161.0
37	85165.0
38	96648.0
39	62796.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.98700809756579	33.49364908902385	20.06153409371451	26.457808719695848
2	23.17573322470745	32.92984372685528	20.143157803782156	23.751265244655112
3	21.29237026613341	32.01717029575865	21.585073816224757	25.10538562188318
4	21.445279711647657	33.04433293833012	18.716054411692095	26.794332938330122
5	18.795209351700983	36.535328099540806	17.4634622031304	27.206000345627807
6	20.81558904853602	36.31684194934084	20.50220954920259	22.365359452920554
7	80.48761294623019	8.820606823680443	5.8850849256900215	4.806695304399349
8	82.59856564459587	6.3084789907668	4.7249172962030315	6.3680380684343065
9	75.99013109169012	11.283050659161606	7.764899027304597	4.961919221843678
10	46.188373327408286	27.726139337382115	10.948995210586085	15.136492124623512
11	37.31885399693873	25.298258282723545	19.86650125907273	17.516386461265
12	34.208358021033916	25.168184960252805	23.473682911173654	17.149774107539624
13	24.523681676788627	33.912722806497804	20.918197304103096	20.645398212610477
14	18.753548856959462	33.2009455389325	27.611187231521257	20.434318372586777
15	18.188354811632845	28.144132967955365	31.616149459339354	22.051362761072436
16	20.307639608946822	25.086406952056485	32.32622944748926	22.27972399150743
17	19.189132474201354	25.92548017577643	26.471541253147684	28.413846096874533
18	23.641713573297782	27.04012985730509	25.406421270922824	23.9117352984743
19	24.79092603564904	30.291037130301685	23.985181207722313	20.932855626326962
20	28.666277835382413	28.3104663506641	22.394213202982275	20.629042610971215
21	23.448995210586084	28.945094553893252	24.13222732434701	23.473682911173654
22	22.93302226830593	26.996309188762158	24.15305757171777	25.91761097121414
23	23.240538438749812	28.74188391843184	21.56316348195329	26.45441416086506
24	21.325544363797956	28.93151631857009	27.143972497901547	22.59896681973041
25	21.383714758307413	26.507338418999655	25.751586184762754	26.35736063793018
26	23.43510837900558	31.705333777711942	21.32106971806646	23.538488125216016
27	20.422128820421666	29.790339702760082	24.541271663457266	25.246259813360982
28	21.086690860613242	28.05973189157162	27.123759443045476	23.729817804769667
29	19.980527576161556	30.22823779193206	26.813311608156816	22.977923023749568
30	23.467665284155434	26.70175406112675	24.12327803288402	25.7073026218338
31	29.988767096232653	28.725682614921244	18.702013281982914	22.58353700686318
32	25.833209894830393	27.348880412778353	22.752339159630672	24.065570532760578
33	27.536661235372538	26.29116674072977	21.39837308053128	24.773798943366415
34	20.47351009726954	30.367106107737126	23.482323606379303	25.677060188614032
35	23.142404828914234	26.52261393373821	24.689706463240015	25.64527477410754
36	28.43760800869007	28.78045845059991	23.19255172073273	19.589381819977287
37	22.908334567718363	31.092461610625588	22.080216511134154	23.918987310521896
38	26.769645237742555	27.73694020638918	22.62025996148719	22.873154594381077
39	23.682911173653288	27.302899570434008	25.061873549597593	23.952315706315115
40	26.171894287266085	27.00988742408532	21.415345874685233	25.40287241396336
41	20.64894706956994	25.584481311410656	26.682929689428725	27.08364192959068
42	25.340535969979754	26.368161506937245	21.1147731200316	27.176529403051397
43	23.783204957290277	24.1072310275021	24.91714190490298	27.192422110304648
44	23.231589147286822	24.615797659605985	24.472608996198094	27.680004196909103
45	26.117118451587416	27.46306102799585	23.440971707895127	22.9788488125216
46	22.351164025082703	25.737236458796225	27.96098108922135	23.95061842689972
47	22.374617340640892	27.681701476324495	24.37077223127438	25.572908951760233
48	23.86745173554535	23.04056806399052	29.810398459487487	23.281581740976645
49	24.149971609144323	22.854021626425713	27.031489162099444	25.964517602330517
50	22.23652051547919	24.851102305831237	24.773798943366415	28.138578235323163
51	20.669931615069373	25.77827976102306	26.889997778106945	26.66179084580062
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3384.0
1	2784.5
2	2185.0
3	1548.5
4	912.0
5	854.0
6	796.0
7	911.5
8	1027.0
9	1082.5
10	1138.0
11	1188.5
12	1239.0
13	1367.0
14	1495.0
15	1660.0
16	1825.0
17	1833.5
18	1842.0
19	1824.0
20	1806.0
21	1764.5
22	1723.0
23	2078.5
24	2434.0
25	2101.0
26	2513.5
27	3259.0
28	3858.0
29	4457.0
30	5877.5
31	7298.0
32	7466.0
33	7634.0
34	8456.0
35	9278.0
36	10181.5
37	11085.0
38	12716.5
39	14348.0
40	20518.5
41	26689.0
42	30831.0
43	34973.0
44	46782.0
45	58591.0
46	59557.0
47	60523.0
48	60675.0
49	60827.0
50	57612.0
51	54397.0
52	49337.5
53	44278.0
54	41019.5
55	37761.0
56	35597.5
57	33434.0
58	32345.5
59	31257.0
60	30791.0
61	30325.0
62	27361.5
63	24398.0
64	21316.0
65	18234.0
66	16455.5
67	14677.0
68	12940.5
69	11204.0
70	10284.5
71	9365.0
72	7864.5
73	6364.0
74	5046.5
75	3217.5
76	2706.0
77	2204.5
78	1703.0
79	1265.5
80	828.0
81	648.5
82	469.0
83	336.0
84	203.0
85	146.0
86	89.0
87	69.0
88	49.0
89	44.0
90	39.0
91	39.5
92	40.0
93	24.5
94	9.0
95	5.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	648096.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.014166595530167
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.06935723515865	22.15062146187223
2	9.397156287327862	5.265070035148729
3	3.376985364253833	2.8381029175462205
4	1.5803106741627442	1.7708434599475884
5	0.8234872095486878	1.1534653938792598
6	0.48439571432384654	0.8141965343537446
7	0.35478927090901935	0.6957388019086367
8	0.23493008306218086	0.526509638816454
9	0.19931692652316343	0.5025327826436051
>10	2.6663836821275844	18.97419734365733
>50	1.504558280297213	30.252801656111185
>100	0.2983653101840315	10.810941105536456
>500	0.006089087962939418	1.0360452919183685
>1k	0.0038748741582341753	3.208933576660201
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCC	4325	0.6673394065076779	No Hit
CTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGCT	4295	0.6627104626475091	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGT	3518	0.5428208166691355	No Hit
AATCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCT	3283	0.5065607564311461	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2836	0.4375894929146299	No Hit
AAACTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCT	1288	0.19873598972991657	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTT	1148	0.1771342517157952	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	789	0.12174122352244111	No Hit
AATGACTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTT	762	0.11757517404828914	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	701	0.10816298819927912	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9109761516812324	0.0
2	0.0	0.0	0.0	1.1063175825803584	0.0
3	0.0	0.0	0.0	1.4497852170048882	0.0
4	0.0	0.0	0.0	3.339782995111835	0.0
5	0.0	0.0	0.0	3.571538784377623	0.0
6	0.0	0.0	0.0	4.80947267071545	0.0
7	0.0	0.0	0.0	5.7776934281341035	0.0
8	0.0	0.0	0.0	6.200316002567521	0.0
9	0.0	0.0	0.0	7.642232015010122	0.0
10	0.0	0.0	0.0	8.753024243321978	0.0
11	1.5429812867229545E-4	0.0	0.0	10.776952797116477	0.0
12	1.5429812867229545E-4	0.0	0.0	11.377944008295067	0.0
13	1.5429812867229545E-4	0.0	0.0	11.635158988791783	0.0
14	1.5429812867229545E-4	0.0	0.0	11.96689996543722	0.0
15	1.5429812867229545E-4	0.0	0.0	12.233218535525602	0.0
16	1.5429812867229545E-4	0.0	0.0	12.689323803880907	0.0
17	1.5429812867229545E-4	0.0	0.0	13.242482595171087	0.0
18	1.5429812867229545E-4	0.0	0.0	13.936361279810399	0.0
19	1.5429812867229545E-4	0.0	0.0	14.254832617390017	1.5429812867229545E-4
20	1.5429812867229545E-4	0.0	0.0	14.558799930874438	1.5429812867229545E-4
21	1.5429812867229545E-4	0.0	0.0	14.918314570680886	1.5429812867229545E-4
22	1.5429812867229545E-4	0.0	0.0	15.289092973880413	1.5429812867229545E-4
23	1.5429812867229545E-4	0.0	0.0	15.67807855626327	1.5429812867229545E-4
24	1.5429812867229545E-4	0.0	0.0	15.94424282822298	1.5429812867229545E-4
25	1.5429812867229545E-4	0.0	0.0	16.170598182985238	1.5429812867229545E-4
26	1.5429812867229545E-4	0.0	0.0	16.38723275564114	1.5429812867229545E-4
27	3.085962573445909E-4	0.0	0.0	16.631023798943367	1.5429812867229545E-4
28	3.085962573445909E-4	0.0	0.0	16.851978719202094	1.5429812867229545E-4
29	3.085962573445909E-4	0.0	0.0	17.096695551276355	1.5429812867229545E-4
30	3.085962573445909E-4	0.0	0.0	17.408069174937047	1.5429812867229545E-4
31	3.085962573445909E-4	0.0	0.0	17.645379696835036	1.5429812867229545E-4
32	3.085962573445909E-4	0.0	0.0	17.877906976744185	1.5429812867229545E-4
33	3.085962573445909E-4	0.0	0.0	18.125864069520564	1.5429812867229545E-4
34	4.628943860168864E-4	0.0	0.0	18.36286599516121	1.5429812867229545E-4
35	4.628943860168864E-4	0.0	0.0	18.650014812620352	1.5429812867229545E-4
36	4.628943860168864E-4	0.0	0.0	18.894268750308598	1.5429812867229545E-4
37	4.628943860168864E-4	0.0	0.0	19.135436725423393	1.5429812867229545E-4
38	4.628943860168864E-4	0.0	0.0	19.416722213992987	1.5429812867229545E-4
39	4.628943860168864E-4	0.0	0.0	19.665450797412728	1.5429812867229545E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAT	20	7.031006E-4	45.000004	18
CGAACCG	20	7.031006E-4	45.000004	1
CAACGGA	20	7.031006E-4	45.000004	3
TTAGCTT	30	2.1640299E-6	45.000004	44
CGAAACG	45	3.8380676E-10	45.000004	1
ACGCATG	30	2.1640299E-6	45.000004	1
CGTGCGT	20	7.031006E-4	45.000004	17
CCGATAT	20	7.031006E-4	45.000004	45
TAGACGT	20	7.031006E-4	45.000004	26
TCCGAAC	20	7.031006E-4	45.000004	35
TCACACG	20	7.031006E-4	45.000004	1
GGGTATA	30	2.1640299E-6	45.000004	7
CGACCGA	30	2.1640299E-6	45.000004	28
TCGTGGG	80	0.0	45.000004	3
TAACCGC	20	7.031006E-4	45.000004	24
GCCGAAC	20	7.031006E-4	45.000004	22
CGCTCAC	20	7.031006E-4	45.000004	25
CTACGAC	20	7.031006E-4	45.000004	29
TACGACA	20	7.031006E-4	45.000004	30
TGCGACC	20	7.031006E-4	45.000004	18
>>END_MODULE