##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552993_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	799612
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14571817331406	31.0	31.0	34.0	28.0	34.0
2	31.322701510232463	31.0	31.0	34.0	28.0	34.0
3	31.562073855820074	31.0	31.0	34.0	30.0	34.0
4	35.445867495735435	37.0	35.0	37.0	33.0	37.0
5	35.020176035377155	35.0	35.0	37.0	32.0	37.0
6	35.014224398833434	35.0	35.0	37.0	32.0	37.0
7	35.08630060579381	36.0	35.0	37.0	32.0	37.0
8	35.41534519241832	37.0	35.0	37.0	33.0	37.0
9	36.18459828016588	39.0	37.0	39.0	32.0	39.0
10	35.63990285288365	37.0	35.0	39.0	30.0	39.0
11	36.03071864854454	37.0	35.0	39.0	31.0	39.0
12	35.93340895334237	37.0	35.0	39.0	31.0	39.0
13	35.714891722485405	37.0	35.0	39.0	30.0	39.0
14	36.66498001530742	38.0	35.0	40.0	31.0	41.0
15	36.67279005317579	38.0	35.0	40.0	31.0	41.0
16	36.67616043781234	38.0	35.0	40.0	31.0	41.0
17	36.66812779197911	38.0	35.0	40.0	31.0	41.0
18	36.50030389739023	38.0	35.0	40.0	31.0	41.0
19	36.50190717497986	38.0	35.0	40.0	31.0	41.0
20	36.43944062870492	38.0	35.0	40.0	31.0	41.0
21	36.45598740389089	38.0	35.0	40.0	31.0	41.0
22	36.436791844044365	38.0	35.0	40.0	31.0	41.0
23	36.34388678509077	38.0	35.0	40.0	30.0	41.0
24	36.00476731214639	38.0	34.0	40.0	30.0	41.0
25	36.091758252752584	38.0	34.0	40.0	30.0	41.0
26	35.991974857806035	38.0	34.0	40.0	30.0	41.0
27	35.80391364811934	38.0	34.0	40.0	29.0	41.0
28	35.83126441324042	38.0	34.0	40.0	29.0	41.0
29	35.53606624212743	38.0	34.0	40.0	28.0	41.0
30	35.396784940696236	38.0	34.0	40.0	27.0	41.0
31	35.30278685162304	38.0	34.0	40.0	27.0	41.0
32	35.07924093185195	38.0	34.0	40.0	26.0	41.0
33	35.13797691880562	38.0	34.0	40.0	27.0	41.0
34	35.04713536064992	38.0	34.0	40.0	26.0	41.0
35	34.99285278360004	38.0	34.0	40.0	26.0	41.0
36	34.80810568125541	38.0	33.0	40.0	25.0	41.0
37	34.61092880046823	37.0	33.0	40.0	24.0	41.0
38	34.616205109478095	37.0	33.0	40.0	24.0	41.0
39	34.52197440758768	37.0	33.0	40.0	24.0	41.0
40	34.44474695227185	37.0	33.0	40.0	24.0	41.0
41	34.59178326488347	37.0	33.0	40.0	24.0	41.0
42	34.47271926884539	37.0	33.0	40.0	24.0	41.0
43	34.3512853734061	37.0	33.0	40.0	23.0	41.0
44	34.125483359429325	37.0	33.0	40.0	23.0	41.0
45	33.867079283452476	37.0	33.0	40.0	23.0	41.0
46	33.7488732035037	37.0	32.0	40.0	22.0	41.0
47	33.688435141043406	37.0	32.0	40.0	22.0	41.0
48	33.666763380239416	37.0	32.0	40.0	22.0	41.0
49	33.53845365002026	36.0	32.0	40.0	20.0	41.0
50	33.27575999359689	36.0	32.0	39.0	20.0	40.0
51	31.330907990375334	35.0	28.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	1.0
10	5.0
11	3.0
12	8.0
13	14.0
14	22.0
15	56.0
16	160.0
17	367.0
18	776.0
19	1429.0
20	2536.0
21	3782.0
22	5273.0
23	6739.0
24	8191.0
25	9171.0
26	10272.0
27	12207.0
28	14939.0
29	18772.0
30	23168.0
31	29406.0
32	37858.0
33	50189.0
34	74816.0
35	86204.0
36	85535.0
37	110187.0
38	128780.0
39	78741.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.146551077272477	36.149157341310534	20.05134990470378	25.652941676713205
2	20.208050904688772	34.263993036622765	21.058838536690295	24.469117521998168
3	19.169672291061165	36.10838756797047	20.463049579045837	24.258890561922534
4	17.830272682250893	33.64081579566089	19.854254313341972	28.67465720874624
5	16.638819827616395	37.13601096531818	18.712075356547924	27.5130938505175
6	19.341505630230664	37.59260741459608	21.135125535884907	21.930761419288356
7	78.39302061499828	8.761124145210427	6.989639975388064	5.856215264403235
8	81.22189261791969	6.926359284252863	5.461523839061945	6.3902242587655005
9	73.28491818531988	12.386632516770634	9.795125635933427	4.533323661976058
10	32.40759268245099	42.53587990175235	11.337748808171963	13.7187786076247
11	25.23086196805451	26.312386507456115	32.340310050374434	16.116441474114946
12	26.827886524964605	25.38956393851018	31.29692901057013	16.48562052595509
13	23.132219126276244	32.766016518011234	25.710219456436374	18.39154489927615
14	15.330810443064887	36.057487881622585	30.996283197350717	17.61541847796181
15	13.672881347453513	32.34193583888186	34.17419948675107	19.810983326913554
16	15.902712815715622	30.607344562112626	32.98399723866075	20.505945383511005
17	15.427607389583947	29.460788482413967	28.9317819142284	26.17982221377368
18	18.88028193673932	28.729683896689895	28.42103420158777	23.968999964983016
19	19.94642401564759	32.40521653001706	28.78533588790563	18.863023566429717
20	22.75828776956824	31.468887410394043	26.443825255248793	19.328999564788923
21	18.285493464330198	33.230741909826264	26.634917935198573	21.84884669064496
22	17.94057617944703	29.789322821568458	27.227455315828173	25.04264568315633
23	18.976953822603964	30.55732030034567	24.855429883493493	25.610295993556875
24	14.439253037723295	32.76751724586424	29.15238890862068	23.64084080779178
25	16.330295193168688	31.06944368018489	27.79285453444921	24.807406592197214
26	20.38351100283638	34.4088132744381	23.99513764175625	21.21253808096927
27	15.385336888390869	34.522743530612345	25.892182708613674	24.199736872383106
28	18.873153479437526	29.407012401014494	28.949040284538004	22.770793835009982
29	20.14264418242848	31.084450958714978	26.470713295948535	22.30219156290801
30	18.71657754010695	32.049669089508406	24.281651601026496	24.95210176935814
31	25.90731504779818	31.29580346468037	21.091479367493235	21.705402120028214
32	22.655237790328307	32.223128217185334	22.895729428772952	22.2259045637134
33	22.781549051289876	28.858246249430973	22.795180662621373	25.56502403665778
34	19.299235129037584	28.888886109763234	26.08189972136486	25.729979039834323
35	19.837996428267708	28.138397122604463	26.60190192243238	25.42170452669545
36	23.95962041590171	27.27010099898451	28.712550587034713	20.05772799807907
37	20.0658569406162	31.984262367248117	26.364786921657	21.585093770478682
38	22.014677118402425	29.708158456851574	27.318249350935204	20.9589150738108
39	20.418027743455575	28.058733485740582	28.146526065141593	23.376712705662246
40	22.198266159087158	26.97545809717713	25.615923723005658	25.210352020730053
41	18.50560021610481	23.09420068733336	32.792029134130054	25.60816996243178
42	23.497396237175032	25.29489302311621	27.302991951096285	23.904718788612477
43	21.09548130843459	25.322406367088035	28.525835029989544	25.056277294487828
44	21.6945218430939	26.37129007568671	25.716222367848406	26.217965713370983
45	24.501383170837858	27.838251552002724	24.7900231612332	22.870342115926224
46	19.269845875249498	27.68117537005448	29.78844739698754	23.260531357708487
47	20.308099428222686	25.935578755696515	26.571387122754537	27.184934693326262
48	21.456281296428767	22.59095661395777	34.56751524489378	21.38524684471969
49	22.03556224769013	21.844469567740354	30.515299920461423	25.604668264108092
50	19.334752354892125	23.483014261917027	27.630025562397766	29.552207820793086
51	18.613402500212604	23.181117842153444	31.895594363266184	26.309885294367767
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5144.0
1	4271.5
2	3399.0
3	2524.5
4	1650.0
5	1720.0
6	1790.0
7	1911.5
8	2033.0
9	2247.0
10	2461.0
11	2688.0
12	2915.0
13	3090.5
14	3266.0
15	3430.5
16	3595.0
17	3589.5
18	3584.0
19	3891.0
20	4198.0
21	4068.0
22	3938.0
23	4175.5
24	4413.0
25	4666.5
26	5747.5
27	6575.0
28	7823.5
29	9072.0
30	10487.0
31	11902.0
32	13858.5
33	15815.0
34	17699.5
35	19584.0
36	21163.5
37	22743.0
38	25163.5
39	27584.0
40	31910.5
41	36237.0
42	44452.5
43	52668.0
44	67266.0
45	81864.0
46	87819.5
47	93775.0
48	98294.0
49	102813.0
50	92604.5
51	82396.0
52	72584.0
53	62772.0
54	52261.5
55	41751.0
56	34563.0
57	27375.0
58	22295.0
59	17215.0
60	15414.5
61	13614.0
62	12153.5
63	10693.0
64	8261.0
65	5829.0
66	4625.0
67	3421.0
68	2891.0
69	2361.0
70	1768.5
71	1176.0
72	1142.5
73	1109.0
74	946.5
75	627.5
76	471.0
77	388.5
78	306.0
79	262.5
80	219.0
81	142.5
82	66.0
83	61.0
84	56.0
85	41.0
86	26.0
87	20.0
88	14.0
89	14.0
90	14.0
91	8.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	799612.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.43286556310449
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.16614033966054	26.570513363391857
2	10.45478785528398	7.199766094235416
3	3.932722015806357	4.062446654019649
4	1.9635206104285632	2.704385648370863
5	1.0835193895227617	1.8654338737227154
6	0.7034354240488299	1.4532778433159257
7	0.4756208542459541	1.1463892253281898
8	0.4009468818333963	1.104460006409222
9	0.2705409733953344	0.8383950869609681
>10	2.653392526144217	20.41774283225553
>50	0.6675584357220042	16.286459166808804
>100	0.21698356741487748	10.646012823176191
>500	0.007220750995503404	1.7498660216848791
>1k	0.003249337947976532	2.821590586401087
>5k	3.6103754977517025E-4	1.1332607739186813
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGCT	9116	1.1400529256689493	TruSeq Adapter, Index 13 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4289	0.5363851467961962	No Hit
AATCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCT	3280	0.4101989464890472	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCC	3198	0.39994397282682104	No Hit
AAACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCT	2908	0.3636763830457772	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGT	2709	0.33878931281671615	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTT	2299	0.2875144445055852	No Hit
ACACCTGGGAATGATACCTGTCTCTTATACACATCTGACGCCGAGACTTTC	1701	0.21272817316398454	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCT	1238	0.15482509016873183	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1075	0.13444020349869687	No Hit
CCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC	942	0.11780713646118368	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCT	887	0.1109288004682271	No Hit
ACACCTGGGAATCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATG	822	0.10279985793109657	No Hit
ACACCTGGGAATGATACGGCTGTCTCTTATACACATCTGACGCCGAGACTT	820	0.1025497366222618	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5012130883478485E-4	0.0	0.0	1.5307424100688833	0.0
2	2.5012130883478485E-4	0.0	0.0	1.9078002831373215	0.0
3	2.5012130883478485E-4	0.0	0.0	2.446186400404196	0.0
4	2.5012130883478485E-4	0.0	0.0	5.387612992301266	0.0
5	2.5012130883478485E-4	0.0	0.0	5.805815820673026	0.0
6	2.5012130883478485E-4	0.0	0.0	8.014136856375343	0.0
7	2.5012130883478485E-4	0.0	0.0	9.76673686738068	0.0
8	2.5012130883478485E-4	0.0	0.0	10.74371069968935	0.0
9	2.5012130883478485E-4	0.0	0.0	12.679149387452915	0.0
10	2.5012130883478485E-4	0.0	0.0	15.011905774300535	0.0
11	2.5012130883478485E-4	0.0	0.0	17.854034206590196	0.0
12	2.5012130883478485E-4	0.0	0.0	19.12865239641226	0.0
13	3.751819632521773E-4	0.0	0.0	19.727943052380404	0.0
14	3.751819632521773E-4	0.0	0.0	20.243693191197732	0.0
15	3.751819632521773E-4	0.0	0.0	20.650640560671928	0.0
16	3.751819632521773E-4	0.0	0.0	21.565459247735152	0.0
17	3.751819632521773E-4	0.0	0.0	22.740779277949805	0.0
18	3.751819632521773E-4	0.0	0.0	24.42159447331956	0.0
19	3.751819632521773E-4	0.0	0.0	25.087417397437758	0.0
20	3.751819632521773E-4	0.0	0.0	25.923197750909193	0.0
21	3.751819632521773E-4	0.0	0.0	26.642296513809196	0.0
22	3.751819632521773E-4	0.0	0.0	27.496085601516736	0.0
23	3.751819632521773E-4	0.0	0.0	28.287844604633246	0.0
24	3.751819632521773E-4	0.0	0.0	28.84924188231292	0.0
25	3.751819632521773E-4	0.0	0.0	29.290831053060735	0.0
26	3.751819632521773E-4	0.0	0.0	29.73467131558806	0.0
27	3.751819632521773E-4	0.0	0.0	30.310950811143403	0.0
28	3.751819632521773E-4	0.0	0.0	30.77292486856125	0.0
29	3.751819632521773E-4	0.0	0.0	31.265288665002526	0.0
30	3.751819632521773E-4	0.0	0.0	31.979760183689088	0.0
31	5.002426176695697E-4	0.0	0.0	32.48800668324137	0.0
32	5.002426176695697E-4	0.0	0.0	32.93434815885705	0.0
33	5.002426176695697E-4	0.0	0.0	33.39106966878936	0.0
34	5.002426176695697E-4	0.0	0.0	33.8400374181478	0.0
35	5.002426176695697E-4	0.0	0.0	34.34653306853824	0.0
36	5.002426176695697E-4	0.0	0.0	34.81613582587555	0.0
37	5.002426176695697E-4	0.0	0.0	35.27735951936689	0.0
38	5.002426176695697E-4	0.0	0.0	35.706692745981805	0.0
39	5.002426176695697E-4	0.0	0.0	36.15741134450209	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCGCG	125	0.0	45.000004	1
GACAGCG	20	7.0319965E-4	45.000004	9
ATCTCGC	20	7.0319965E-4	45.000004	11
ATCTCGA	30	2.1646683E-6	45.000004	15
CCGGGTA	30	2.1646683E-6	45.000004	5
ATCTCCG	20	7.0319965E-4	45.000004	1
AGATCGC	20	7.0319965E-4	45.000004	42
CGAGTAG	20	7.0319965E-4	45.000004	1
GCGGTCC	20	7.0319965E-4	45.000004	12
CCTAGCG	40	6.8102963E-9	45.000004	1
CGCATCG	20	7.0319965E-4	45.000004	21
GACAACG	30	2.1646683E-6	45.000004	1
CGACACG	20	7.0319965E-4	45.000004	1
AGACGCG	20	7.0319965E-4	45.000004	1
TTCCCGG	30	2.1646683E-6	45.000004	2
ACTAGCG	30	2.1646683E-6	45.000004	1
CGCTCGA	30	2.1646683E-6	45.000004	21
GTTGTGC	20	7.0319965E-4	45.000004	33
CGTAGTG	20	7.0319965E-4	45.000004	38
CGCTATT	30	2.1646683E-6	45.000004	29
>>END_MODULE