##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552992_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	806249
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.940630003882177	31.0	31.0	33.0	28.0	34.0
2	31.160854773153208	31.0	31.0	34.0	28.0	34.0
3	31.187221317483804	31.0	31.0	34.0	28.0	34.0
4	35.24158293529666	37.0	35.0	37.0	33.0	37.0
5	34.88507892722968	35.0	35.0	37.0	32.0	37.0
6	34.92303370298754	35.0	35.0	37.0	32.0	37.0
7	34.95023001578917	35.0	35.0	37.0	32.0	37.0
8	35.334378089151116	37.0	35.0	37.0	33.0	37.0
9	35.699473735781375	39.0	35.0	39.0	32.0	39.0
10	35.31822303035415	37.0	35.0	39.0	29.0	39.0
11	35.85332198861642	37.0	35.0	39.0	30.0	39.0
12	35.827586762898314	37.0	35.0	39.0	31.0	39.0
13	35.59975020124056	37.0	35.0	39.0	30.0	39.0
14	36.54594548334323	38.0	35.0	40.0	31.0	41.0
15	36.59846399809488	38.0	35.0	40.0	31.0	41.0
16	36.606767884363265	38.0	35.0	40.0	31.0	41.0
17	36.60430338518249	38.0	35.0	40.0	31.0	41.0
18	36.44828210639641	38.0	35.0	40.0	31.0	41.0
19	36.456263511644664	38.0	35.0	40.0	31.0	41.0
20	36.35112353627726	38.0	35.0	40.0	30.0	41.0
21	36.351700281178644	38.0	35.0	40.0	30.0	41.0
22	36.35669377574422	38.0	35.0	40.0	30.0	41.0
23	36.270851808808445	38.0	35.0	40.0	30.0	41.0
24	35.883387142185605	38.0	34.0	40.0	29.0	41.0
25	36.02267785758494	38.0	34.0	40.0	30.0	41.0
26	35.89962530186084	38.0	34.0	40.0	30.0	41.0
27	35.72376647909021	38.0	34.0	40.0	29.0	41.0
28	35.70443870317979	38.0	34.0	40.0	29.0	41.0
29	35.43090906159264	38.0	34.0	40.0	27.0	41.0
30	35.347975625396124	38.0	34.0	40.0	27.0	41.0
31	35.31475263845289	38.0	34.0	40.0	27.0	41.0
32	35.08755483727732	38.0	34.0	40.0	27.0	41.0
33	35.07071016522191	38.0	34.0	40.0	27.0	41.0
34	35.070251249924034	38.0	34.0	40.0	26.0	41.0
35	34.95060458989717	38.0	34.0	40.0	25.0	41.0
36	34.82312040076949	38.0	33.0	40.0	25.0	41.0
37	34.61454091726005	37.0	33.0	40.0	24.0	41.0
38	34.642813820544276	37.0	33.0	40.0	25.0	41.0
39	34.484757190396515	37.0	33.0	40.0	24.0	41.0
40	34.37807550769055	37.0	33.0	40.0	24.0	41.0
41	34.53260097066787	37.0	33.0	40.0	24.0	41.0
42	34.47363531613683	37.0	33.0	40.0	24.0	41.0
43	34.37616914873693	37.0	33.0	40.0	24.0	41.0
44	34.27278793524085	37.0	33.0	40.0	23.0	41.0
45	34.06660225315008	37.0	33.0	40.0	23.0	41.0
46	33.828117616269914	37.0	32.0	40.0	23.0	41.0
47	33.72105515789787	37.0	32.0	40.0	22.0	41.0
48	33.63296822693734	36.0	32.0	40.0	22.0	41.0
49	33.51614327583662	36.0	32.0	39.0	21.0	41.0
50	33.3918789356638	36.0	32.0	39.0	20.0	40.0
51	31.555183324258387	35.0	29.0	38.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	14.0
11	11.0
12	12.0
13	19.0
14	31.0
15	66.0
16	171.0
17	351.0
18	859.0
19	1599.0
20	2663.0
21	3851.0
22	5385.0
23	6747.0
24	7959.0
25	9108.0
26	10522.0
27	12317.0
28	15425.0
29	18729.0
30	23775.0
31	30579.0
32	39322.0
33	52124.0
34	77571.0
35	87774.0
36	88976.0
37	111902.0
38	125600.0
39	72778.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.19177450142574	35.91198252648996	20.117358285095545	25.778884686988757
2	20.328831415604856	34.08711204603044	21.116429291695248	24.467627246669455
3	19.449946604584937	36.042835401966386	20.382040783926552	24.12517720952212
4	18.028673523936153	33.58193312487829	19.758040009972106	28.631353341213444
5	16.91388144357388	37.10987548511688	18.476674079595757	27.49956899171348
6	19.232519978319353	37.59979857339358	21.06520442195897	22.102477026328092
7	78.93231495480924	8.85173190912485	6.5526902979104475	5.663262838155458
8	81.79135726059815	7.003295507963421	5.149277704530486	6.05606952690794
9	73.16858687576668	13.392140641414748	9.08354615013476	4.355726332683823
10	32.95681607046955	42.67713820420242	11.109099050045334	13.256946675282698
11	25.62917907495079	26.52108715793756	31.90825663039582	15.941477136715829
12	27.269243124642635	25.54669835249408	30.825960714369877	16.358097808493405
13	23.156369806350146	33.01114171924554	25.623225579194518	18.209262895209793
14	15.463957164598034	36.461564603490984	30.487851767878162	17.586626464032822
15	13.9357692226595	32.32376102171909	34.274151037706716	19.46631871791469
16	16.102097490973634	30.576161954929553	33.072661175393705	20.249079378703104
17	15.563182093869262	29.25225333612817	28.831911729502917	26.352652840499648
18	19.2545975250822	28.826826451877768	28.315321941484576	23.60325408155545
19	20.306133713034065	32.25703225678419	28.63879521090879	18.798038819272954
20	23.085672044244397	31.467821975593147	26.141923896959874	19.304582083202586
21	18.625015348856248	33.101932529528725	26.72325795132769	21.549794170287342
22	18.212859798895874	29.762021410259116	27.098080121649765	24.927038669195248
23	18.863651303753556	30.802022700183194	24.841085074214046	25.493240921849203
24	14.724111285719424	32.68059867361075	29.306454953742577	23.28883508692724
25	16.372609454399324	31.31923264400948	27.849088805071386	24.45906909651981
26	20.120583095296862	34.60097314849383	24.080525991350065	21.19791776485924
27	15.55449991255803	34.70069420241141	26.033396630569467	23.711409254461092
28	18.708612351767258	29.83854863695955	29.153276469180117	22.299562542093078
29	19.859001375505585	31.117557975265708	26.81466891741881	22.2087717318099
30	18.8563334652198	32.03811725657955	24.602697181639915	24.50285209656074
31	25.756062953256375	31.546209669717417	21.196553422081763	21.50117395494444
32	22.733671607654706	32.227016715679646	22.99760991951618	22.041701757149465
33	22.827687228139197	29.100811287827955	22.933733871297825	25.137767612735022
34	19.32318675744094	29.279788254000934	25.992838440729848	25.404186547828278
35	19.815962562434187	28.378205740410223	26.60852912685783	25.197302570297765
36	24.03389027459259	27.580809402554294	28.434391856610052	19.95090846624306
37	20.07816443803341	32.129280160347484	26.453862268356303	21.3386931332628
38	21.892740332081033	29.956130178145955	27.120529761897377	21.030599727875632
39	20.384397375996745	28.50198883967608	27.934546275406234	23.17906750892094
40	22.276368714875925	27.284871051002852	25.544837885070244	24.893922349050975
41	18.55382146210414	23.62235488044016	32.32661373843565	25.497209919020055
42	23.3673468122131	25.493737046495564	27.106638271799405	24.03227786949193
43	21.281018643123897	25.678047352616872	28.237182309683483	24.803751694575745
44	21.584398864370684	26.88214186932325	25.80195448304432	25.731504783261748
45	24.092184920539435	28.50992683401778	24.756247759687145	22.64164048575564
46	19.496210227857645	28.130019386070554	29.343540271057698	23.030230115014096
47	20.38104853463384	26.38626528529028	26.500001860467425	26.732684319608456
48	21.537887178774795	22.9871913019427	33.99222820741483	21.482693311867674
49	21.9779497400927	22.520462040883153	30.103231135790555	25.39835708323359
50	19.46879934114647	24.017518161262835	27.634886989007118	28.878795508583575
51	18.73428680221619	23.810138059085965	31.318612488201538	26.136962650496308
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5549.0
1	4591.0
2	3633.0
3	2703.5
4	1774.0
5	1745.0
6	1716.0
7	1840.0
8	1964.0
9	2166.0
10	2368.0
11	2611.5
12	2855.0
13	3004.5
14	3154.0
15	3303.5
16	3453.0
17	3527.0
18	3601.0
19	3920.0
20	4239.0
21	4163.0
22	4087.0
23	4280.0
24	4473.0
25	4796.5
26	6055.0
27	6990.0
28	8261.0
29	9532.0
30	11011.0
31	12490.0
32	14443.5
33	16397.0
34	18416.0
35	20435.0
36	22061.0
37	23687.0
38	26082.5
39	28478.0
40	32718.5
41	36959.0
42	45404.0
43	53849.0
44	68026.5
45	82204.0
46	87584.5
47	92965.0
48	97261.5
49	101558.0
50	91801.0
51	82044.0
52	72454.5
53	62865.0
54	52392.0
55	41919.0
56	34709.0
57	27499.0
58	22369.0
59	17239.0
60	15689.0
61	14139.0
62	12507.0
63	10875.0
64	8334.5
65	5794.0
66	4627.0
67	3460.0
68	2923.0
69	2386.0
70	1804.5
71	1223.0
72	1188.5
73	1154.0
74	1007.0
75	680.0
76	500.0
77	424.0
78	348.0
79	281.5
80	215.0
81	144.5
82	74.0
83	66.0
84	58.0
85	42.5
86	27.0
87	23.5
88	20.0
89	14.0
90	8.0
91	8.0
92	8.0
93	5.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	806249.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.997238495622675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.77001992757359	26.099686767914243
2	10.464612926922085	7.1153588288189225
3	4.105147061029347	4.1869099108025765
4	2.0290905752393544	2.7593390486253018
5	1.169422664229147	1.9878570608992392
6	0.7108366547093867	1.4499889968951363
7	0.509164338909729	1.2117127024366077
8	0.3895126037533987	1.0593882309484213
9	0.2854064514929684	0.8732728079636228
>10	2.6482577514261774	20.12077379756479
>50	0.6594002037063765	15.919002264289164
>100	0.24846964045629805	11.844938243824085
>500	0.006983614154836777	1.5854897820196219
>1k	0.003308027757554263	2.726247680826142
>5k	3.675586397282514E-4	1.0600338761721668
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGCT	8483	1.052156343759806	TruSeq Adapter, Index 13 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4665	0.5786053688128605	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCC	3080	0.3820159776942359	No Hit
AATCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCT	2832	0.3512562496201546	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGT	2658	0.329674827503662	No Hit
AAACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCT	2623	0.3253337368480457	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTT	2113	0.2620778444376365	No Hit
ACACCTGGGAATGATACCTGTCTCTTATACACATCTGACGCCGAGACTTTC	1661	0.2060157593993915	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCT	1110	0.13767458936383176	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1075	0.13333349870821545	No Hit
CCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC	932	0.11559704260098308	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	822	0.10195361482618892	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2403116158903762E-4	0.0	0.0	1.374513332729715	0.0
2	1.2403116158903762E-4	0.0	0.0	1.7053044406876783	0.0
3	1.2403116158903762E-4	0.0	0.0	2.202793429821308	0.0
4	1.2403116158903762E-4	0.0	0.0	4.837959488942002	0.0
5	1.2403116158903762E-4	0.0	0.0	5.228409585624292	0.0
6	1.2403116158903762E-4	0.0	0.0	7.241683400537551	0.0
7	1.2403116158903762E-4	0.0	0.0	8.838336543673233	0.0
8	1.2403116158903762E-4	0.0	0.0	9.760880323572493	0.0
9	1.2403116158903762E-4	0.0	0.0	11.56454147540028	0.0
10	1.2403116158903762E-4	0.0	0.0	13.756668225324931	0.0
11	1.2403116158903762E-4	0.0	0.0	16.44491962160573	0.0
12	1.2403116158903762E-4	0.0	0.0	17.651122668059124	0.0
13	1.2403116158903762E-4	0.0	0.0	18.224518728085243	0.0
14	1.2403116158903762E-4	0.0	0.0	18.71878290701756	0.0
15	1.2403116158903762E-4	0.0	0.0	19.09484538895552	0.0
16	1.2403116158903762E-4	0.0	0.0	19.971993763713197	0.0
17	1.2403116158903762E-4	0.0	0.0	21.08765406220659	0.0
18	1.2403116158903762E-4	0.0	0.0	22.690632794583312	0.0
19	1.2403116158903762E-4	0.0	0.0	23.33757933343173	0.0
20	1.2403116158903762E-4	0.0	0.0	24.153952439010776	0.0
21	1.2403116158903762E-4	0.0	0.0	24.860681997745115	0.0
22	1.2403116158903762E-4	0.0	0.0	25.670853545244707	0.0
23	1.2403116158903762E-4	0.0	0.0	26.413924234324632	0.0
24	1.2403116158903762E-4	0.0	0.0	26.949614821227684	0.0
25	2.4806232317807524E-4	0.0	0.0	27.405677402390577	0.0
26	2.4806232317807524E-4	0.0	0.0	27.841522904214454	0.0
27	2.4806232317807524E-4	0.0	0.0	28.387135984044633	0.0
28	2.4806232317807524E-4	0.0	0.0	28.84890399864062	0.0
29	2.4806232317807524E-4	0.0	0.0	29.326175908435236	0.0
30	2.4806232317807524E-4	0.0	0.0	30.030920968584148	0.0
31	2.4806232317807524E-4	0.0	0.0	30.531386705595914	0.0
32	2.4806232317807524E-4	0.0	0.0	30.960534524693983	0.0
33	2.4806232317807524E-4	0.0	0.0	31.405186238990684	0.0
34	2.4806232317807524E-4	0.0	0.0	31.844132519854288	0.0
35	2.4806232317807524E-4	0.0	0.0	32.337032356009125	0.0
36	2.4806232317807524E-4	0.0	0.0	32.796939903181276	0.0
37	2.4806232317807524E-4	0.0	0.0	33.24853736252696	0.0
38	2.4806232317807524E-4	0.0	0.0	33.67644487000914	0.0
39	2.4806232317807524E-4	0.0	0.0	34.10708106304628	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTGCG	35	1.2113196E-7	45.000004	1
TAGCGCG	35	1.2113196E-7	45.000004	1
GCGTTGG	35	1.2113196E-7	45.000004	2
ACTAGCG	35	1.2113196E-7	45.000004	1
TTTGCGC	35	1.2113196E-7	45.000004	13
TCATACG	35	1.2113196E-7	45.000004	1
CGATCGT	35	1.2113196E-7	45.000004	27
TCACGCG	45	3.8380676E-10	45.0	1
AACGTAA	25	3.8897662E-5	45.0	15
CTATGCG	25	3.8897662E-5	45.0	1
CGTGGAC	55	1.8189894E-12	45.0	45
CAGCGCG	85	0.0	45.0	1
TCGTTAC	20	7.0320326E-4	45.0	20
ACGCCTC	20	7.0320326E-4	45.0	23
TCCGCGA	25	3.8897662E-5	45.0	17
ATAGGCG	25	3.8897662E-5	45.0	1
TTTACGT	20	7.0320326E-4	45.0	37
GCGAATC	55	1.8189894E-12	45.0	14
GCGGTCC	20	7.0320326E-4	45.0	12
ACTTGGC	20	7.0320326E-4	45.0	35
>>END_MODULE