SRR3552991_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552991_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	553029
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCC	4753	0.8594486003446474	No Hit
CTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGCT	4347	0.7860347287393609	Illumina PCR Primer Index 2 (95% over 24bp)
AATGATACCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGT	3775	0.6826043480540803	No Hit
AATCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCT	3689	0.6670536264825172	Illumina PCR Primer Index 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2579	0.46634082480303923	No Hit
AAACTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCT	1188	0.21481694450019798	Illumina PCR Primer Index 2 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTT	1140	0.2061374719951395	No Hit
AATGACTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTT	840	0.15189076883852384	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCT	774	0.13995649414406838	No Hit
AACTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTG	643	0.11626876709901288	Illumina PCR Primer Index 2 (95% over 22bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	624	0.11283314256576057	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCT	617	0.11156738615877286	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	610	0.11030162975178517	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	580	0.1048769594361236	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCT	570	0.10306873599756974	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	30	2.1634514E-6	45.000004	1
CTCACGG	85	0.0	45.000004	2
ACTTCGC	30	2.1634514E-6	45.000004	13
GTCTAGG	60	0.0	45.000004	2
CATACGA	60	0.0	45.000004	18
TTAACGG	85	0.0	45.000004	2
ACCAACG	30	2.1634514E-6	45.000004	1
ACCCGAT	30	2.1634514E-6	45.000004	41
ATTGCGG	60	0.0	45.000004	2
CCCGATT	30	2.1634514E-6	45.000004	42
AAGTACG	55	1.8189894E-12	45.0	1
AGACTAT	20	7.030108E-4	45.0	27
AATCCCG	20	7.030108E-4	45.0	1
GTACCAC	20	7.030108E-4	45.0	44
ATCTACG	45	3.8380676E-10	45.0	1
CAACGAT	20	7.030108E-4	45.0	10
GCGAAGA	20	7.030108E-4	45.0	9
GACGTTA	20	7.030108E-4	45.0	9
CGGTTTA	20	7.030108E-4	45.0	29
ACACCCC	20	7.030108E-4	45.0	22