##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552991_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	553029
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31995790455835	31.0	31.0	34.0	30.0	34.0
2	31.489614468680667	31.0	31.0	34.0	30.0	34.0
3	31.685991150554493	31.0	31.0	34.0	30.0	34.0
4	35.530675606523346	37.0	35.0	37.0	33.0	37.0
5	35.10612644183217	37.0	35.0	37.0	32.0	37.0
6	35.11440810518074	37.0	35.0	37.0	32.0	37.0
7	35.09831130013073	37.0	35.0	37.0	32.0	37.0
8	35.44491156883274	37.0	35.0	37.0	33.0	37.0
9	36.288301336819586	39.0	37.0	39.0	32.0	39.0
10	35.9416594789785	38.0	35.0	39.0	30.0	39.0
11	36.2238598699164	38.0	35.0	39.0	32.0	39.0
12	35.77223617567976	37.0	35.0	39.0	31.0	39.0
13	35.440837641425674	37.0	35.0	39.0	30.0	39.0
14	36.409667485791886	38.0	35.0	40.0	31.0	41.0
15	36.50247093732878	38.0	35.0	40.0	31.0	41.0
16	36.52119870748189	38.0	35.0	40.0	31.0	41.0
17	36.47785559165975	38.0	35.0	40.0	31.0	41.0
18	36.291693563990314	38.0	35.0	40.0	30.0	41.0
19	36.30134947715219	38.0	35.0	40.0	30.0	41.0
20	36.2982592232957	38.0	34.0	40.0	30.0	41.0
21	36.348348820767086	38.0	35.0	40.0	31.0	41.0
22	36.32105911263243	38.0	35.0	40.0	31.0	41.0
23	36.28412253245309	38.0	35.0	40.0	31.0	41.0
24	35.908102468405815	38.0	34.0	40.0	30.0	41.0
25	36.041298015113135	38.0	34.0	40.0	30.0	41.0
26	35.89743214189491	38.0	34.0	40.0	30.0	41.0
27	35.738039054009825	38.0	34.0	40.0	30.0	41.0
28	35.74512186521864	38.0	34.0	40.0	30.0	41.0
29	35.38722200824912	37.0	34.0	40.0	29.0	41.0
30	35.26983033439476	37.0	34.0	40.0	27.0	41.0
31	35.177030499304735	37.0	34.0	40.0	27.0	41.0
32	34.925206453911095	37.0	34.0	40.0	26.0	41.0
33	35.0108999708876	37.0	34.0	40.0	27.0	41.0
34	34.8256492878312	37.0	33.0	40.0	25.0	41.0
35	34.617794003569436	37.0	33.0	40.0	25.0	41.0
36	34.480497406103474	37.0	33.0	40.0	24.0	41.0
37	34.1350381263912	36.0	33.0	40.0	23.0	41.0
38	34.05658835250954	36.0	33.0	40.0	23.0	41.0
39	33.934900339765186	36.0	33.0	40.0	23.0	41.0
40	33.812351612664074	36.0	33.0	40.0	22.0	41.0
41	33.93716966018057	36.0	33.0	40.0	23.0	41.0
42	33.70769887293433	36.0	33.0	40.0	22.0	41.0
43	33.53279665261677	35.0	33.0	40.0	22.0	41.0
44	33.32240623909415	35.0	32.0	39.0	21.0	41.0
45	32.8473787088923	35.0	31.0	39.0	19.0	41.0
46	32.7539514202691	35.0	31.0	39.0	18.0	40.0
47	32.65832894839149	35.0	31.0	39.0	18.0	40.0
48	32.64917752956897	35.0	31.0	39.0	18.0	40.0
49	32.52204676427457	35.0	31.0	39.0	18.0	40.0
50	32.25008272622232	35.0	31.0	39.0	15.0	40.0
51	30.32669896153728	34.0	27.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	2.0
11	12.0
12	14.0
13	16.0
14	23.0
15	67.0
16	132.0
17	280.0
18	506.0
19	985.0
20	1751.0
21	2631.0
22	3782.0
23	4894.0
24	6244.0
25	7377.0
26	8815.0
27	10402.0
28	12304.0
29	14858.0
30	17934.0
31	21944.0
32	27711.0
33	36934.0
34	58612.0
35	61018.0
36	52007.0
37	67275.0
38	79673.0
39	54819.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.90208289257887	34.66183509363885	20.75677767350356	25.67930434027872
2	21.348970849629946	35.31406128792523	19.44328416773804	23.893683694706787
3	19.198089069470136	32.16847579421694	23.097161270023815	25.53627386628911
4	21.994144972505964	32.491062855654945	18.05420692224097	27.460585249598125
5	17.092774519961882	37.07906818629764	18.137023555726735	27.69113373801374
6	20.689692583933212	35.89522430107643	21.631053706044348	21.784029408946004
7	77.26864233159563	9.699129702059023	7.227649906243615	5.80457806010173
8	80.00122959193821	5.934227680646042	5.597355654043459	8.467187073372283
9	74.04494158534182	10.950601143882146	9.687557072052279	5.316900198723755
10	41.82728211359621	30.32698104439369	12.485963665558225	15.359773176451869
11	34.5108484365196	23.80905883778247	24.440852107213185	17.239240618484743
12	33.92281417430189	21.915668075272727	28.114981312010762	16.04653643841462
13	24.85800925448756	31.891998430462053	23.536017098560833	19.71397521648955
14	17.559476989452634	32.706060622499	31.550786667606946	18.18367572044142
15	16.566762321686564	28.420209428438653	33.37040191382369	21.642626336051094
16	18.74802225561408	24.56525787978569	35.121304669375384	21.565415195224844
17	18.19741821857443	25.97711874060854	28.40791350905649	27.417549531760542
18	21.593804303210142	26.703301273531764	26.378363521623637	25.32453090163445
19	22.947440369311558	30.996204539002477	26.644714834122624	19.41164025756335
20	26.673284764451772	29.95068974683064	23.421014087868812	19.95501140084878
21	21.082257892443256	29.773122205164647	25.45743532436816	23.68718457802394
22	19.986836133367326	28.847854271656644	25.562673928491996	25.602635666484037
23	22.356874594279866	28.387299761856973	22.44294602995503	26.81287961390813
24	19.6651531836486	30.41305248006886	26.751038372309587	23.170755963972955
25	20.773051684450543	26.147815033208023	26.715597192913933	26.363536089427498
26	21.906626958079954	31.77392143992449	22.426672019008045	23.89277958298751
27	18.25166492173105	29.747445432337184	26.51777754873614	25.483112097195626
28	23.74920664196633	26.74633699136935	27.188990089127334	22.315466277536984
29	20.282480665570883	27.235642253842023	28.12004433763871	24.36183274294838
30	23.760417627285367	28.39688334608131	25.548931430358984	22.293767596274336
31	29.81453052190753	26.818304284223792	20.736887215679467	22.63027797818921
32	25.943124139963725	25.734816799842324	23.44614839366471	24.87591066652924
33	28.258192608344228	24.54120850805292	25.713298940923536	21.487299942679318
34	21.319677629925373	24.728902100974814	28.117693647168597	25.83372662193122
35	22.28816210361482	24.893450433883213	27.586618423265325	25.23176903923664
36	25.466838086248643	25.52361630221923	28.54913575960754	20.46040985192458
37	22.023076547522823	29.483444810308317	25.45164900936479	23.041829632804067
38	24.21608993380094	26.898589404895585	26.634950427554433	22.250370233749045
39	21.71423198421783	24.005251080865563	30.54776512624112	23.732751808675495
40	23.34072896719702	24.511553643660637	26.27131669406125	25.87640069508109
41	19.592824246106446	20.71988991535706	31.750595357567146	27.93669048096935
42	24.693280099235302	22.8734840306747	23.627513204551658	28.805722665538337
43	22.249646944373623	23.236575297136316	27.34666717296923	27.16711058552083
44	21.846232295232255	23.153577841306692	25.812389585356282	29.187800278104763
45	25.099949550566063	26.253957749051132	25.32977474960626	23.31631795077654
46	20.57487039558504	24.368342347327175	32.425966811867006	22.630820445220774
47	21.935377710752963	24.50649061803269	26.68467657211466	26.873455099099687
48	22.167191955575564	20.05229382184298	33.6224682611581	24.158045961423362
49	22.93279375945927	20.23528603382463	30.212882145420945	26.619038061295157
50	20.16512696440874	22.158693305414364	27.55009230980654	30.12608742037036
51	19.051080503915706	23.259901379493662	31.52709894056189	26.161919176028743
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3111.0
1	2570.5
2	2030.0
3	1531.0
4	1032.0
5	1014.5
6	997.0
7	1027.5
8	1058.0
9	1132.5
10	1207.0
11	1291.5
12	1376.0
13	1455.0
14	1534.0
15	1615.5
16	1697.0
17	1705.5
18	1714.0
19	1620.0
20	1526.0
21	1647.0
22	1768.0
23	1805.5
24	1843.0
25	2107.0
26	2729.5
27	3088.0
28	3827.0
29	4566.0
30	5151.5
31	5737.0
32	6885.5
33	8034.0
34	8572.0
35	9110.0
36	10421.5
37	11733.0
38	12370.0
39	13007.0
40	16120.5
41	19234.0
42	25395.0
43	31556.0
44	43557.0
45	55558.0
46	57280.5
47	59003.0
48	59003.5
49	59004.0
50	53557.0
51	48110.0
52	43569.0
53	39028.0
54	35325.5
55	31623.0
56	29470.5
57	27318.0
58	25454.0
59	23590.0
60	22452.5
61	21315.0
62	19422.0
63	17529.0
64	15910.0
65	14291.0
66	11701.0
67	9111.0
68	7270.5
69	5430.0
70	4869.5
71	4309.0
72	3961.5
73	3614.0
74	2630.0
75	1454.5
76	1263.0
77	960.0
78	657.0
79	657.0
80	657.0
81	419.0
82	181.0
83	188.0
84	195.0
85	166.5
86	138.0
87	97.0
88	56.0
89	55.5
90	55.0
91	31.5
92	8.0
93	6.5
94	5.0
95	4.5
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	553029.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.125908474331695
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.06924375694159	25.722971965444604
2	9.183426722507551	5.900518527360189
3	3.174569892925494	3.0595782547648023
4	1.481127700055794	1.9033029172315938
5	0.8061959096718814	1.2949888003249725
6	0.524884846324429	1.0117441519549357
7	0.3628808030708035	0.8160512826581232
8	0.2524832055412062	0.6489001882018134
9	0.20860948863874307	0.6031592404996857
>10	2.6693965934580968	21.325425463454664
>50	1.0624351337570472	24.45371874855077
>100	0.1957707823026449	8.351632632375582
>500	0.005048530202647003	1.038728001316521
>1k	0.00392663460205878	3.8692798258617325
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCC	4753	0.8594486003446474	No Hit
CTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGCT	4347	0.7860347287393609	Illumina PCR Primer Index 2 (95% over 24bp)
AATGATACCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGT	3775	0.6826043480540803	No Hit
AATCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCT	3689	0.6670536264825172	Illumina PCR Primer Index 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2579	0.46634082480303923	No Hit
AAACTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCT	1188	0.21481694450019798	Illumina PCR Primer Index 2 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTT	1140	0.2061374719951395	No Hit
AATGACTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTT	840	0.15189076883852384	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCT	774	0.13995649414406838	No Hit
AACTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTG	643	0.11626876709901288	Illumina PCR Primer Index 2 (95% over 22bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	624	0.11283314256576057	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCT	617	0.11156738615877286	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	610	0.11030162975178517	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	580	0.1048769594361236	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCT	570	0.10306873599756974	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.6164468771077103E-4	0.0	0.0	1.2823920626223941	0.0
2	3.6164468771077103E-4	0.0	0.0	1.5626666955982418	0.0
3	3.6164468771077103E-4	0.0	0.0	2.0919336960629553	0.0
4	3.6164468771077103E-4	0.0	0.0	4.8820224617515535	0.0
5	3.6164468771077103E-4	0.0	0.0	5.28851109073846	0.0
6	3.6164468771077103E-4	0.0	0.0	7.117529098835685	0.0
7	3.6164468771077103E-4	0.0	0.0	8.631373761592972	0.0
8	3.6164468771077103E-4	0.0	0.0	9.438022237531847	0.0
9	3.6164468771077103E-4	0.0	0.0	11.584021814407564	0.0
10	3.6164468771077103E-4	0.0	0.0	13.255724383350602	0.0
11	3.6164468771077103E-4	0.0	0.0	16.223019046017477	0.0
12	3.6164468771077103E-4	0.0	0.0	17.112303333098264	0.0
13	3.6164468771077103E-4	0.0	0.0	17.543022156161793	0.0
14	3.6164468771077103E-4	0.0	0.0	18.043538403953498	0.0
15	3.6164468771077103E-4	0.0	0.0	18.369199445237047	0.0
16	5.424670315661565E-4	0.0	0.0	18.955244661672353	0.0
17	5.424670315661565E-4	0.0	0.0	19.677087458343053	0.0
18	5.424670315661565E-4	0.0	0.0	20.61645953467178	0.0
19	5.424670315661565E-4	0.0	0.0	21.050613982268562	0.0
20	5.424670315661565E-4	0.0	0.0	21.459634124069442	0.0
21	5.424670315661565E-4	0.0	0.0	21.955991457952475	0.0
22	5.424670315661565E-4	0.0	0.0	22.416003500720578	0.0
23	5.424670315661565E-4	0.0	0.0	22.917423860231562	0.0
24	5.424670315661565E-4	0.0	0.0	23.239468454638004	0.0
25	5.424670315661565E-4	0.0	0.0	23.5248061132418	0.0
26	5.424670315661565E-4	0.0	0.0	23.788083445895243	0.0
27	5.424670315661565E-4	0.0	0.0	24.075590972625307	0.0
28	5.424670315661565E-4	0.0	0.0	24.356046427945007	0.0
29	5.424670315661565E-4	0.0	0.0	24.657115630464226	0.0
30	5.424670315661565E-4	0.0	0.0	25.080059092741973	0.0
31	7.232893754215421E-4	0.0	0.0	25.385106386826006	0.0
32	7.232893754215421E-4	0.0	0.0	25.679123517934865	0.0
33	7.232893754215421E-4	0.0	0.0	25.981458476861068	0.0
34	7.232893754215421E-4	0.0	0.0	26.276741364376914	0.0
35	7.232893754215421E-4	0.0	0.0	26.607103786600703	0.0
36	7.232893754215421E-4	0.0	0.0	26.911066146621607	0.0
37	7.232893754215421E-4	0.0	0.0	27.215570973674076	0.0
38	7.232893754215421E-4	0.0	0.0	27.525138826354496	0.0
39	7.232893754215421E-4	0.0	0.0	27.833260100284072	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	30	2.1634514E-6	45.000004	1
CTCACGG	85	0.0	45.000004	2
ACTTCGC	30	2.1634514E-6	45.000004	13
GTCTAGG	60	0.0	45.000004	2
CATACGA	60	0.0	45.000004	18
TTAACGG	85	0.0	45.000004	2
ACCAACG	30	2.1634514E-6	45.000004	1
ACCCGAT	30	2.1634514E-6	45.000004	41
ATTGCGG	60	0.0	45.000004	2
CCCGATT	30	2.1634514E-6	45.000004	42
AAGTACG	55	1.8189894E-12	45.0	1
AGACTAT	20	7.030108E-4	45.0	27
AATCCCG	20	7.030108E-4	45.0	1
GTACCAC	20	7.030108E-4	45.0	44
ATCTACG	45	3.8380676E-10	45.0	1
CAACGAT	20	7.030108E-4	45.0	10
GCGAAGA	20	7.030108E-4	45.0	9
GACGTTA	20	7.030108E-4	45.0	9
CGGTTTA	20	7.030108E-4	45.0	29
ACACCCC	20	7.030108E-4	45.0	22
>>END_MODULE