##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552989_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	591200
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.069181326116375	31.0	31.0	34.0	28.0	34.0
2	31.262007780784845	31.0	31.0	34.0	28.0	34.0
3	31.515065967523682	31.0	31.0	34.0	30.0	34.0
4	35.39744079837618	37.0	35.0	37.0	33.0	37.0
5	34.986711772665764	35.0	35.0	37.0	32.0	37.0
6	35.002298714479025	36.0	35.0	37.0	32.0	37.0
7	35.06797023004059	36.0	35.0	37.0	32.0	37.0
8	35.38742557510149	37.0	35.0	37.0	33.0	37.0
9	36.278924221921514	39.0	37.0	39.0	32.0	39.0
10	35.760037212449255	37.0	35.0	39.0	30.0	39.0
11	36.07904600811908	37.0	35.0	39.0	31.0	39.0
12	35.91680649526387	37.0	35.0	39.0	31.0	39.0
13	35.74368572395129	37.0	35.0	39.0	30.0	39.0
14	36.73745602165088	38.0	35.0	40.0	31.0	41.0
15	36.736003044654936	38.0	35.0	40.0	31.0	41.0
16	36.74146650879567	38.0	35.0	40.0	31.0	41.0
17	36.70812246278755	38.0	35.0	40.0	31.0	41.0
18	36.53187753721245	38.0	35.0	40.0	31.0	41.0
19	36.553386332882276	38.0	35.0	40.0	31.0	41.0
20	36.51596752368065	38.0	35.0	40.0	31.0	41.0
21	36.55433694181326	38.0	35.0	40.0	31.0	41.0
22	36.54882442489851	38.0	35.0	40.0	31.0	41.0
23	36.494844384303114	38.0	35.0	40.0	31.0	41.0
24	36.120849120433014	38.0	34.0	40.0	30.0	41.0
25	36.21248308525034	38.0	35.0	40.0	30.0	41.0
26	36.097667456021654	38.0	35.0	40.0	30.0	41.0
27	35.95940629228687	38.0	34.0	40.0	30.0	41.0
28	35.92362821380244	38.0	34.0	40.0	30.0	41.0
29	35.644406292286874	38.0	34.0	40.0	29.0	41.0
30	35.45243403247632	38.0	34.0	40.0	27.0	41.0
31	35.25500845737483	38.0	34.0	40.0	27.0	41.0
32	35.145348443843034	38.0	34.0	40.0	27.0	41.0
33	35.05336265223275	37.0	34.0	40.0	27.0	41.0
34	35.00728856562923	38.0	34.0	40.0	26.0	41.0
35	34.94312415426252	37.0	33.0	40.0	26.0	41.0
36	34.54646143437077	37.0	33.0	40.0	24.0	41.0
37	34.365015223274696	37.0	33.0	40.0	24.0	41.0
38	34.26264546684709	37.0	33.0	40.0	24.0	41.0
39	34.26178958051421	37.0	33.0	40.0	24.0	41.0
40	34.28889546684709	37.0	33.0	40.0	24.0	41.0
41	34.36801589986468	37.0	33.0	40.0	24.0	41.0
42	34.30845230040595	37.0	33.0	40.0	24.0	41.0
43	34.0751099458728	37.0	33.0	40.0	23.0	41.0
44	33.89074932341001	37.0	32.0	40.0	23.0	41.0
45	33.704113667117724	36.0	32.0	40.0	23.0	41.0
46	33.64345060893099	36.0	32.0	39.0	22.0	41.0
47	33.59889715832206	36.0	32.0	39.0	22.0	41.0
48	33.59784336941813	36.0	32.0	39.0	22.0	41.0
49	33.38054634641407	36.0	32.0	39.0	22.0	40.0
50	33.05371447902571	35.0	31.0	39.0	20.0	40.0
51	31.071958728010827	34.0	28.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	20.0
9	21.0
10	20.0
11	17.0
12	22.0
13	21.0
14	21.0
15	52.0
16	108.0
17	233.0
18	522.0
19	990.0
20	1655.0
21	2572.0
22	3532.0
23	4589.0
24	5609.0
25	6661.0
26	7476.0
27	9349.0
28	11376.0
29	14030.0
30	17950.0
31	22644.0
32	28964.0
33	38567.0
34	57678.0
35	64965.0
36	64488.0
37	80694.0
38	90524.0
39	55824.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.734776725304464	35.11366711772666	19.85081190798376	25.30074424898511
2	20.641407307171853	35.56765899864682	20.947902571041947	22.843031123139376
3	19.942828146143437	33.59117050067659	22.231901217861978	24.234100135318
4	20.432510148849797	33.69367388362652	18.63058186738836	27.243234100135318
5	16.292625169147495	37.86079161028417	18.952131258457374	26.89445196211096
6	20.076454668470905	36.501860622462786	20.9404600811908	22.481224627875505
7	77.05395805142084	9.162212449255751	7.860284167794316	5.923545331529093
8	79.95906630581867	6.36129905277402	5.7523680649526385	7.927266576454668
9	72.57002706359947	11.792625169147497	10.725473612990529	4.911874154262517
10	33.658998646820024	39.593876860622466	11.927604871447903	14.819519621109608
11	25.93792286874154	25.553619756427604	31.44198240866035	17.0664749661705
12	26.23782138024357	25.411535859269286	31.57966847090663	16.770974289580515
13	22.3323748308525	33.11332882273342	25.392422192151553	19.161874154262517
14	15.554296346414073	34.9096752368065	32.534167794316645	17.00186062246279
15	13.783491204330176	31.53636671177267	33.01962110960758	21.66052097428958
16	15.820872801082544	28.66136671177267	33.036535859269286	22.481224627875505
17	15.00236806495264	29.041610284167795	29.557510148849797	26.39851150202977
18	18.101657645466847	28.817320703653586	28.17794316644114	24.903078484438428
19	19.5723951285521	32.783491204330176	28.475473612990527	19.1686400541272
20	22.576962110960757	31.505412719891744	26.413734776725306	19.503890392422193
21	18.262347767253047	32.42642083897158	26.521481732070367	22.789749661705006
22	17.517591339648174	30.14918809201624	27.15984438430311	25.173376184032474
23	19.764039242219216	30.09793640054127	23.617219215155615	26.520805142083898
24	14.907814614343708	33.90155615696887	27.5585250338295	23.632104194857916
25	18.506596752368065	30.118741542625173	27.06596752368065	24.308694181326114
26	22.06258457374831	33.34489174560217	23.40037212449256	21.19215155615697
27	16.37026387009472	31.699763193504737	26.539073071718537	25.390899864682005
28	21.36400541271989	30.42997293640054	27.446887686062247	20.75913396481732
29	20.724458728010823	31.461434370771315	26.340663058186735	21.473443843031124
30	21.904769959404604	31.571041948579158	25.49255751014885	21.03163058186739
31	26.986468200270636	33.609945872801084	19.711772665764546	19.691813261163734
32	25.007104194857916	32.374661705006766	21.23866711772666	21.37956698240866
33	24.47801082543978	30.420500676589985	25.114174560216508	19.98731393775372
34	24.00575101488498	30.545669824086602	22.418301759133964	23.030277401894452
35	26.094215155615696	28.66880920162382	25.545331529093367	19.691644113667117
36	26.599458728010827	29.950778078484436	26.82628552097429	16.623477672530445
37	22.89732746955345	34.60571718538566	21.70652909336942	20.790426251691475
38	23.40882949932341	32.351150202976996	25.863497970230043	18.37652232746955
39	22.521481732070363	29.0228349120433	27.36062246278755	21.095060893098783
40	26.768944519621108	27.40409336941813	24.27249661705007	21.55446549391069
41	25.10081190798376	23.544993234100136	29.11366711772666	22.240527740189446
42	30.403416779431662	25.837787550744252	22.25456698240866	21.504228687415427
43	23.514039242219216	26.792794316644112	26.011671177266578	23.681495263870094
44	22.656292286874155	27.243403247631935	24.28941136671177	25.810893098782138
45	24.943504736129903	29.006089309878213	25.109100135317995	20.941305818673882
46	21.081190798376184	26.60808525033829	31.321887686062244	20.988836265223274
47	22.729194857916102	26.387347767253043	25.937415426251693	24.94604194857916
48	24.476995940460082	22.869079837618404	32.35267253044655	20.301251691474967
49	24.38531799729364	21.94519621109608	29.04854533152909	24.620940460081194
50	20.86468200270636	24.58423545331529	25.631258457374827	28.91982408660352
51	20.328822733423547	23.902909336941814	31.376860622462786	24.391407307171853
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4320.0
1	3573.0
2	2826.0
3	2039.5
4	1253.0
5	1196.5
6	1140.0
7	1300.0
8	1460.0
9	1546.0
10	1632.0
11	1767.5
12	1903.0
13	2032.5
14	2162.0
15	2161.0
16	2160.0
17	2191.5
18	2223.0
19	2271.5
20	2320.0
21	2490.5
22	2661.0
23	2779.0
24	2897.0
25	2998.0
26	3973.0
27	4847.0
28	5400.0
29	5953.0
30	6633.0
31	7313.0
32	9311.5
33	11310.0
34	12498.0
35	13686.0
36	14594.5
37	15503.0
38	16973.0
39	18443.0
40	22277.0
41	26111.0
42	32352.0
43	38593.0
44	51031.5
45	63470.0
46	66104.0
47	68738.0
48	70821.0
49	72904.0
50	66841.0
51	60778.0
52	53320.0
53	45862.0
54	38719.5
55	31577.0
56	26644.0
57	21711.0
58	18841.5
59	15972.0
60	14032.0
61	12092.0
62	10380.0
63	8668.0
64	7297.0
65	5926.0
66	4727.0
67	3528.0
68	2850.5
69	2173.0
70	1774.0
71	1375.0
72	1138.5
73	902.0
74	774.5
75	572.5
76	498.0
77	363.0
78	228.0
79	162.5
80	97.0
81	72.5
82	48.0
83	68.5
84	89.0
85	65.5
86	42.0
87	40.5
88	39.0
89	24.5
90	10.0
91	8.5
92	7.0
93	4.0
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	591200.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.85593372535263
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.39534444590504	29.677289637203724
2	10.101411994104591	7.647967659626111
3	3.6410568067083546	4.135068154949866
4	1.7056427340017912	2.582747931900043
5	0.9517749035829566	1.8015163835745145
6	0.633412152123746	1.4387045070977702
7	0.46248185314264856	1.2255377667222458
8	0.36847582771130133	1.1159197210586782
9	0.29577211266620634	1.0077056545409495
>10	2.8244913566932905	23.930495752250746
>50	0.5127281136844992	13.568120545501342
>100	0.09856755978461942	5.812733568784014
>500	0.0053040839345983555	1.3719048484544785
>1k	0.003094048961849041	3.516687913156211
>5k	4.42006994549863E-4	1.167599955179333
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGCT	6978	1.1803112313937754	Illumina Single End Adapter 2 (95% over 21bp)
AATCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCT	4380	0.7408660351826792	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCC	4352	0.7361299052774019	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGT	3994	0.675575101488498	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3592	0.6075778078484438	No Hit
AAACTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCT	2053	0.34725981055480376	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTT	1636	0.27672530446549387	No Hit
AATGACTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTT	1010	0.17083897158322056	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCT	891	0.1507104194857916	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	868	0.14682002706359945	No Hit
AACTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTG	805	0.1361637347767253	No Hit
CCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGC	752	0.12719891745602166	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	732	0.12381596752368065	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCT	707	0.1195872801082544	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCT	636	0.10757780784844384	No Hit
GCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGC	633	0.1070703653585927	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6914749661705008E-4	0.0	0.0	1.5637686062246279	0.0
2	1.6914749661705008E-4	0.0	0.0	1.9903585926928282	0.0
3	1.6914749661705008E-4	0.0	0.0	2.6133288227334237	0.0
4	1.6914749661705008E-4	0.0	0.0	5.97479702300406	0.0
5	1.6914749661705008E-4	0.0	0.0	6.437077131258458	0.0
6	1.6914749661705008E-4	0.0	0.0	8.719722598105548	0.0
7	1.6914749661705008E-4	0.0	0.0	10.516914749661705	0.0
8	1.6914749661705008E-4	0.0	0.0	11.453484438430312	0.0
9	1.6914749661705008E-4	0.0	0.0	13.729194857916102	0.0
10	1.6914749661705008E-4	0.0	0.0	16.031461434370772	0.0
11	1.6914749661705008E-4	0.0	0.0	19.316644113667117	0.0
12	1.6914749661705008E-4	0.0	0.0	20.647665764546684	0.0
13	1.6914749661705008E-4	0.0	0.0	21.293809201623816	0.0
14	1.6914749661705008E-4	0.0	0.0	21.876522327469555	0.0
15	1.6914749661705008E-4	0.0	0.0	22.320196211096075	0.0
16	1.6914749661705008E-4	0.0	0.0	23.18978349120433	0.0
17	1.6914749661705008E-4	0.0	0.0	24.36874154262517	0.0
18	1.6914749661705008E-4	0.0	0.0	25.818504736129906	0.0
19	1.6914749661705008E-4	0.0	0.0	26.4484100135318	0.0
20	1.6914749661705008E-4	0.0	0.0	27.057171853856563	0.0
21	1.6914749661705008E-4	0.0	0.0	27.764715832205685	0.0
22	1.6914749661705008E-4	0.0	0.0	28.543978349120433	0.0
23	1.6914749661705008E-4	0.0	0.0	29.347936400541272	0.0
24	1.6914749661705008E-4	0.0	0.0	29.930987821380242	0.0
25	1.6914749661705008E-4	0.0	0.0	30.374154262516914	0.0
26	1.6914749661705008E-4	0.0	0.0	30.78332205683356	0.0
27	1.6914749661705008E-4	0.0	0.0	31.246786197564276	0.0
28	1.6914749661705008E-4	0.0	0.0	31.718031123139376	0.0
29	1.6914749661705008E-4	0.0	0.0	32.221583220568334	0.0
30	1.6914749661705008E-4	0.0	0.0	32.89901894451962	0.0
31	1.6914749661705008E-4	0.0	0.0	33.37855209742896	0.0
32	3.3829499323410016E-4	0.0	0.0	33.80006765899865	0.0
33	3.3829499323410016E-4	0.0	0.0	34.24052774018944	0.0
34	3.3829499323410016E-4	0.0	0.0	34.70449932341001	0.0
35	3.3829499323410016E-4	0.0	0.0	35.21244925575102	0.0
36	3.3829499323410016E-4	0.0	0.0	35.651725304465494	0.0
37	3.3829499323410016E-4	0.0	0.0	36.097428958051424	0.0
38	3.3829499323410016E-4	0.0	0.0	36.51995940460081	0.0
39	3.3829499323410016E-4	0.0	0.0	36.942320703653586	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGTG	20	7.030503E-4	45.000004	45
ACGTTGT	20	7.030503E-4	45.000004	31
GCAACGT	20	7.030503E-4	45.000004	44
CCTACGC	20	7.030503E-4	45.000004	28
TAACGTT	20	7.030503E-4	45.000004	29
CCGTCAT	20	7.030503E-4	45.000004	17
AGTGCCG	20	7.030503E-4	45.000004	45
CTAGTAG	20	7.030503E-4	45.000004	1
CTACGCC	20	7.030503E-4	45.000004	29
CTACGAA	20	7.030503E-4	45.000004	10
CGCTACG	20	7.030503E-4	45.000004	1
ACTAAGC	20	7.030503E-4	45.000004	22
CTCGCGA	20	7.030503E-4	45.000004	18
CGTGTTA	20	7.030503E-4	45.000004	38
TACTAGA	20	7.030503E-4	45.000004	30
CGACTCG	40	6.8048394E-9	45.000004	1
TAGTGCG	20	7.030503E-4	45.000004	1
TAAGACG	20	7.030503E-4	45.000004	1
CCGCACG	20	7.030503E-4	45.000004	1
GCGTAAG	20	7.030503E-4	45.000004	1
>>END_MODULE