FastQCFastQC Report
Sat 18 Jun 2016
SRR3552987_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552987_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466158
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCC66701.430845335701629No Hit
AATCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCT63411.36026840684918No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGT59881.284543009022692No Hit
CTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGCT33640.7216437345277781No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31690.6798124241137125No Hit
AAACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCT15560.33379240515018516No Hit
AATGACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTT14860.31877603730923854No Hit
AATGATCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCT12230.26235739813539616No Hit
AAAAACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTT11610.24905718661912915No Hit
AACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTG9910.21258886471968733No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7550.16196225314163867No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCACGCACATTCGTAT6990.14994915886888135No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCT6440.138150584136709No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA6350.13621990827144442No Hit
CGTAGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC5650.12120354043049782No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5640.12098902088991285No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAC950.045.00000421
AATCACG302.1627147E-645.0000041
ATCACCG600.045.0000041
TCTAGCG351.2098462E-745.0000041
GCATTTA302.1627147E-645.00000411
AACGAGT302.1627147E-645.00000430
TTAACGG351.2098462E-745.0000042
CATAACG351.2098462E-745.0000041
ATACGAG302.1627147E-645.0000041
TAGTGCC302.1627147E-645.00000417
GCGTAAG351.2098462E-745.0000041
GATAGCG351.2098462E-745.0000041
GACTAGT351.2098462E-745.00000416
AAATGCG302.1627147E-645.0000041
GCATAAA207.028968E-445.09
TCACGGA207.028968E-445.043
CTATGCC207.028968E-445.016
CCTTATC207.028968E-445.042
GTCGCTC207.028968E-445.037
TACCCGA207.028968E-445.015