##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552987_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	466158
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.341345638174182	31.0	31.0	34.0	30.0	34.0
2	31.51324229124031	31.0	31.0	34.0	30.0	34.0
3	31.70817405257445	31.0	31.0	34.0	30.0	34.0
4	35.52922399701389	37.0	35.0	37.0	33.0	37.0
5	35.097621407333996	37.0	35.0	37.0	32.0	37.0
6	35.1266330300027	37.0	35.0	37.0	32.0	37.0
7	35.0625646240116	37.0	35.0	37.0	32.0	37.0
8	35.40471256526757	37.0	35.0	37.0	33.0	37.0
9	36.30916127150022	39.0	37.0	39.0	32.0	39.0
10	36.02461611728212	38.0	35.0	39.0	31.0	39.0
11	36.23344874484617	38.0	35.0	39.0	32.0	39.0
12	35.77511916560479	37.0	35.0	39.0	31.0	39.0
13	35.54737449534278	37.0	35.0	39.0	30.0	39.0
14	36.538868795558585	38.0	35.0	40.0	31.0	41.0
15	36.62597874540392	38.0	35.0	40.0	31.0	41.0
16	36.62227613813342	38.0	35.0	40.0	31.0	41.0
17	36.57305891993702	38.0	35.0	40.0	31.0	41.0
18	36.345788337859695	38.0	35.0	40.0	31.0	41.0
19	36.347974291978254	38.0	35.0	40.0	30.0	41.0
20	36.33001257084508	38.0	35.0	40.0	30.0	41.0
21	36.40217050871164	38.0	35.0	40.0	31.0	41.0
22	36.35547604031251	38.0	35.0	40.0	31.0	41.0
23	36.292806301726024	38.0	35.0	40.0	31.0	41.0
24	35.930575041080495	38.0	34.0	40.0	30.0	41.0
25	35.97377498616349	38.0	34.0	40.0	30.0	41.0
26	35.859412473882244	38.0	34.0	40.0	30.0	41.0
27	35.78489267587385	38.0	34.0	40.0	30.0	41.0
28	35.65599646471797	38.0	34.0	40.0	29.0	41.0
29	35.44490494639157	37.0	34.0	40.0	29.0	41.0
30	35.40465464499161	37.0	34.0	40.0	28.0	41.0
31	35.23069645914046	37.0	34.0	40.0	27.0	41.0
32	35.133733626796065	37.0	34.0	40.0	27.0	41.0
33	35.174987879645954	37.0	34.0	40.0	27.0	41.0
34	34.88299675217416	37.0	34.0	40.0	26.0	41.0
35	34.87594334967972	37.0	33.0	40.0	26.0	41.0
36	34.740641585041985	37.0	33.0	40.0	25.0	41.0
37	34.55333599337564	37.0	33.0	40.0	25.0	41.0
38	34.581727654572056	37.0	33.0	40.0	25.0	41.0
39	34.348326104024814	36.0	33.0	40.0	24.0	41.0
40	34.25829225284131	36.0	33.0	40.0	24.0	41.0
41	34.30812085172838	36.0	33.0	40.0	24.0	41.0
42	34.256996554816176	36.0	33.0	40.0	24.0	41.0
43	34.29558003938579	36.0	33.0	40.0	24.0	41.0
44	34.1162524294338	36.0	33.0	40.0	24.0	41.0
45	33.831812389790585	35.0	33.0	39.0	23.0	41.0
46	33.68812720150679	35.0	33.0	39.0	23.0	40.0
47	33.58696836694855	35.0	32.0	39.0	23.0	40.0
48	33.53096804087885	35.0	33.0	39.0	23.0	40.0
49	33.41962596372904	35.0	32.0	39.0	22.0	40.0
50	33.18384539147671	35.0	32.0	39.0	22.0	40.0
51	31.09464173091527	34.0	28.0	38.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	7.0
11	12.0
12	12.0
13	18.0
14	32.0
15	61.0
16	110.0
17	256.0
18	446.0
19	853.0
20	1395.0
21	1999.0
22	2730.0
23	3490.0
24	4282.0
25	4771.0
26	5603.0
27	6830.0
28	8544.0
29	10899.0
30	13952.0
31	17983.0
32	23452.0
33	31481.0
34	50138.0
35	53987.0
36	48726.0
37	60191.0
38	68411.0
39	45480.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.21524890702294	38.30289301052433	16.099906040441223	25.38195204201151
2	20.766349606785685	37.57652984610368	18.742572260907245	22.91454828620339
3	19.59442935657009	31.984005423053986	23.790646089952332	24.63091913042359
4	23.183341270556333	31.52321744987751	17.967942199854985	27.325499079711168
5	16.567344119375836	38.47086181080235	19.339365622814583	25.622428447007238
6	21.167715667220126	35.09882915234749	21.903732210967096	21.829722969465287
7	74.97608107122477	11.262275880709973	8.043839213313941	5.717803834751307
8	77.72471994473976	6.979178733390825	5.3237314386967505	9.972369883172657
9	73.00722072773608	10.916041342205862	10.159001883481567	5.917736046576483
10	43.086678765568756	27.67795468489225	12.580712977145089	16.65465357239391
11	35.006585749895955	24.62748681777423	22.106453176819876	18.259474255509932
12	31.76326481579207	23.43175489855371	28.399598419420023	16.405381866234194
13	23.958829409770935	33.62572346715062	21.775449525697297	20.639997597381146
14	17.055590593747187	33.554717499217	31.210447959704652	18.179243947331162
15	16.518004625041293	28.662599376177177	31.64206127536157	23.177334723419957
16	18.144277262215816	25.019628537963523	33.88893894344836	22.947155256372305
17	17.908520287112953	27.56147057435462	26.456694940342118	28.073314198190314
18	20.768923841272702	27.57541434449264	26.25804984576045	25.39761196847421
19	22.606283706382815	30.63296135644996	26.353296521780166	20.407458415387058
20	25.6728405390447	30.566460298868627	23.70548183234011	20.055217329746565
21	21.70894846811596	29.837093860879786	23.98714598912815	24.466811681876102
22	20.839715289665737	29.86498140115583	24.468313318660197	24.826989990518236
23	23.103110962377563	28.955204029535047	20.40724389584647	27.534441112240916
24	19.09481334654774	31.320281964484142	26.843044632935616	22.741860056032504
25	20.10562942178403	27.59214686865827	25.690002102291498	26.612221607266207
26	22.980405785162972	31.914286572363874	21.303935575491572	23.801372066981582
27	19.60944572441104	29.66183139622188	25.44587886510582	25.282844014261258
28	20.851299344857324	30.64261473577628	26.03108817182157	22.474997747544823
29	19.835334800646994	29.624719515700686	25.89937317390241	24.640572509749916
30	24.846940307792636	28.1247988879307	22.539568129260896	24.488692675015766
31	25.797690911665143	30.100738376258697	18.73055916663449	25.371011545441675
32	25.465400143299057	28.583441665701333	20.854088098884926	25.09707009211469
33	24.655803397131447	28.36699144925111	19.902693936390666	27.074511217226778
34	18.090218337988407	31.315133495510107	22.19976917697433	28.394878989527157
35	22.559518446535296	28.034271641803855	25.5816697342961	23.824540177364756
36	24.496415378476826	26.841328476610936	23.671158705846516	24.991097439065722
37	22.525409839582288	30.9787668558729	21.303935575491572	25.191887729053242
38	23.22281286602397	28.047786372860706	23.28266381784717	25.44673694326816
39	21.01969718421651	29.04272800209371	24.863243792877093	25.074331020812686
40	22.80707399637033	26.723557248829792	23.462002153776186	27.007366601023687
41	19.123344445445536	24.807468712325008	29.214558154102257	26.854628688127203
42	23.837411349799854	24.938325632081828	25.43408029037365	25.79018272774467
43	21.93719725929835	23.38327348238151	26.690306720039132	27.989222538281012
44	22.579897802890866	23.70526731279952	24.706859047790665	29.007975836518952
45	25.48213266746468	27.595364661767043	23.32428060872065	23.598222062047633
46	20.276386976089654	25.50487173876668	31.14201622625805	23.076725058885614
47	22.763526529631584	26.216647574427554	24.058151957061767	26.961673938879095
48	22.044027990509655	22.482720451005882	32.56642597574213	22.906825582742332
49	23.208011017723603	20.92037463692568	28.700569334860717	27.17104501049001
50	20.28797103128124	24.22011421020341	25.45488868581039	30.037026072704965
51	20.246568759948342	23.358818254754826	30.48258315849991	25.912029826796925
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3726.0
1	2996.5
2	2267.0
3	1620.0
4	973.0
5	960.0
6	947.0
7	965.5
8	984.0
9	1049.5
10	1115.0
11	1211.0
12	1307.0
13	1344.0
14	1381.0
15	1509.0
16	1637.0
17	1668.0
18	1699.0
19	1644.0
20	1589.0
21	1494.5
22	1400.0
23	1696.5
24	1993.0
25	1793.0
26	1840.5
27	2088.0
28	2447.0
29	2806.0
30	3373.5
31	3941.0
32	4980.0
33	6019.0
34	6952.0
35	7885.0
36	8125.0
37	8365.0
38	9746.0
39	11127.0
40	12648.0
41	14169.0
42	20293.0
43	26417.0
44	38700.5
45	50984.0
46	50376.5
47	49769.0
48	47272.0
49	44775.0
50	42556.0
51	40337.0
52	36758.0
53	33179.0
54	30678.0
55	28177.0
56	24479.5
57	20782.0
58	19860.5
59	18939.0
60	18530.5
61	18122.0
62	16093.0
63	14064.0
64	12792.5
65	11521.0
66	9858.0
67	8195.0
68	7467.0
69	6739.0
70	5992.0
71	5245.0
72	4226.5
73	3208.0
74	2849.5
75	2182.0
76	1873.0
77	1397.0
78	921.0
79	789.5
80	658.0
81	541.0
82	424.0
83	303.0
84	182.0
85	125.5
86	69.0
87	50.0
88	31.0
89	23.0
90	15.0
91	14.5
92	14.0
93	13.0
94	12.0
95	7.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	466158.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.164739115585842
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.53098624045512	23.195004653081206
2	9.07844025236784	5.2954068307348265
3	3.0847964151609992	2.6990184801859494
4	1.4269844775987477	1.664705200446321
5	0.8152430752917103	1.188817580333532
6	0.5258217674733919	0.9201272801794631
7	0.35641376425267296	0.7276300116143185
8	0.3225334963640266	0.7525284221995673
9	0.2512485674654555	0.6594839030965212
>10	3.2213063428169826	23.243739101878315
>50	1.1761246352971202	23.985890500639158
>100	0.19834706529058244	7.995154000449754
>500	0.0051423313223484335	1.0397535269229214
>1k	0.0044077125620129425	2.5622115039091735
>5k	0.0022038562810064713	4.070529004328989
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCC	6670	1.430845335701629	No Hit
AATCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCT	6341	1.36026840684918	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGT	5988	1.284543009022692	No Hit
CTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGCT	3364	0.7216437345277781	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3169	0.6798124241137125	No Hit
AAACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCT	1556	0.33379240515018516	No Hit
AATGACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTT	1486	0.31877603730923854	No Hit
AATGATCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCT	1223	0.26235739813539616	No Hit
AAAAACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTT	1161	0.24905718661912915	No Hit
AACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTG	991	0.21258886471968733	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	755	0.16196225314163867	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCACGCACATTCGTAT	699	0.14994915886888135	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCT	644	0.138150584136709	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	635	0.13621990827144442	No Hit
CGTAGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	565	0.12120354043049782	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	564	0.12098902088991285	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.290390811699038E-4	0.0	0.0	0.9917238361242325	0.0
2	4.290390811699038E-4	0.0	0.0	1.3012755331883181	0.0
3	4.290390811699038E-4	0.0	0.0	1.9156594974236203	0.0
4	4.290390811699038E-4	0.0	0.0	5.311932863964579	0.0
5	4.290390811699038E-4	0.0	0.0	5.688414657691169	0.0
6	4.290390811699038E-4	0.0	0.0	7.51440498715028	0.0
7	4.290390811699038E-4	0.0	0.0	8.92015153660347	0.0
8	4.290390811699038E-4	0.0	0.0	9.482621772017213	0.0
9	4.290390811699038E-4	0.0	0.0	11.847485187425722	0.0
10	4.290390811699038E-4	0.0	0.0	13.298495359942336	0.0
11	4.290390811699038E-4	0.0	0.0	16.546535723939094	0.0
12	4.290390811699038E-4	0.0	0.0	17.338541867778737	0.0
13	4.290390811699038E-4	0.0	0.0	17.695073344230927	0.0
14	4.290390811699038E-4	0.0	0.0	18.171092204788934	0.0
15	4.290390811699038E-4	0.0	0.0	18.496089308775137	0.0
16	4.290390811699038E-4	0.0	0.0	19.015870155612475	0.0
17	4.290390811699038E-4	0.0	0.0	19.60644245084285	0.0
18	4.290390811699038E-4	0.0	0.0	20.450791362585218	0.0
19	4.290390811699038E-4	0.0	0.0	20.809468034443256	0.0
20	4.290390811699038E-4	0.0	0.0	21.178441644249375	0.0
21	4.290390811699038E-4	0.0	0.0	21.573586638006855	0.0
22	4.290390811699038E-4	0.0	0.0	21.96680095589907	0.0
23	4.290390811699038E-4	0.0	0.0	22.353794207114326	0.0
24	4.290390811699038E-4	0.0	0.0	22.62945181676599	0.0
25	4.290390811699038E-4	0.0	0.0	22.883657472359157	0.0
26	4.290390811699038E-4	0.0	0.0	23.124777435976643	0.0
27	4.290390811699038E-4	0.0	0.0	23.36718451683764	0.0
28	4.290390811699038E-4	0.0	0.0	23.60637380458986	0.0
29	4.290390811699038E-4	0.0	0.0	23.851784159019044	0.0
30	4.290390811699038E-4	0.0	0.0	24.204883322821875	0.0
31	4.290390811699038E-4	0.0	0.0	24.47153111176897	0.0
32	4.290390811699038E-4	0.0	0.0	24.728311001849157	0.0
33	4.290390811699038E-4	0.0	0.0	24.98916676320046	0.0
34	4.290390811699038E-4	0.0	0.0	25.24787732914591	0.0
35	4.290390811699038E-4	0.0	0.0	25.557643545750583	0.0
36	4.290390811699038E-4	0.0	0.0	25.80755881053205	0.0
37	4.290390811699038E-4	0.0	0.0	26.051038489095973	0.0
38	4.290390811699038E-4	0.0	0.0	26.304600586067384	0.0
39	6.435586217548556E-4	0.0	0.0	26.555588448551777	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	95	0.0	45.000004	21
AATCACG	30	2.1627147E-6	45.000004	1
ATCACCG	60	0.0	45.000004	1
TCTAGCG	35	1.2098462E-7	45.000004	1
GCATTTA	30	2.1627147E-6	45.000004	11
AACGAGT	30	2.1627147E-6	45.000004	30
TTAACGG	35	1.2098462E-7	45.000004	2
CATAACG	35	1.2098462E-7	45.000004	1
ATACGAG	30	2.1627147E-6	45.000004	1
TAGTGCC	30	2.1627147E-6	45.000004	17
GCGTAAG	35	1.2098462E-7	45.000004	1
GATAGCG	35	1.2098462E-7	45.000004	1
GACTAGT	35	1.2098462E-7	45.000004	16
AAATGCG	30	2.1627147E-6	45.000004	1
GCATAAA	20	7.028968E-4	45.0	9
TCACGGA	20	7.028968E-4	45.0	43
CTATGCC	20	7.028968E-4	45.0	16
CCTTATC	20	7.028968E-4	45.0	42
GTCGCTC	20	7.028968E-4	45.0	37
TACCCGA	20	7.028968E-4	45.0	15
>>END_MODULE