FastQCFastQC Report
Sat 18 Jun 2016
SRR3552986_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552986_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471796
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCC61361.3005621073514824No Hit
AATCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCT57361.2157797013963663No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGT57211.2126003611730494No Hit
CTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGCT32510.6890690044002069No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31250.6623625465243452No Hit
AATGACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTT14480.30691230955752064No Hit
AAACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCT13870.2939829926493654No Hit
AATGATCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCT11940.25307548177602185No Hit
AAAAACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTT10470.22191794758751665No Hit
AACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTG9750.20665711451559574No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7980.1691408998804568No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCACGCACATTCGTAT6790.14391813410880974No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA6060.12844534502200106No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCT5750.12187470856047952No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5690.1206029724711528No Hit
CGTAGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC5340.11318451195008013No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGTC207.029052E-445.00000444
TCGCACA207.029052E-445.00000424
CAAACCG207.029052E-445.0000041
AGATCAT207.029052E-445.00000431
CGAACAG207.029052E-445.0000041
GCGAATT207.029052E-445.0000049
CCTAGCG207.029052E-445.0000041
TAGACGT302.1627711E-645.00000418
TAGCGTG207.029052E-445.00000419
CGTTGAA207.029052E-445.00000420
CGTCTAC207.029052E-445.00000442
GATCAAT207.029052E-445.0000049
TACGGTG207.029052E-445.00000445
GTCTAAG207.029052E-445.00000423
TGCGGTT207.029052E-445.00000435
ACGGGTA406.8012014E-945.0000045
CACCTCG207.029052E-445.00000437
TCGTGTT207.029052E-445.00000442
GTAGCGC207.029052E-445.00000418
GCACAAT406.8012014E-945.00000435