##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552986_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	471796
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14423818769129	31.0	31.0	34.0	28.0	34.0
2	31.345299663413847	31.0	31.0	34.0	28.0	34.0
3	31.32377976922229	31.0	31.0	34.0	28.0	34.0
4	35.332707356569365	37.0	35.0	37.0	33.0	37.0
5	34.97027952759243	35.0	35.0	37.0	32.0	37.0
6	35.045085163926785	36.0	35.0	37.0	32.0	37.0
7	34.91980644176721	36.0	35.0	37.0	32.0	37.0
8	35.31943679047724	37.0	35.0	37.0	33.0	37.0
9	35.80379443657852	39.0	35.0	39.0	32.0	39.0
10	35.68442080899372	38.0	35.0	39.0	30.0	39.0
11	36.066066689840525	37.0	35.0	39.0	31.0	39.0
12	35.665334169853075	37.0	35.0	39.0	31.0	39.0
13	35.423396976659404	37.0	35.0	39.0	30.0	39.0
14	36.4007346395476	38.0	35.0	40.0	31.0	41.0
15	36.54073370694113	38.0	35.0	40.0	31.0	41.0
16	36.54310549474773	38.0	35.0	40.0	31.0	41.0
17	36.50240146164868	38.0	35.0	40.0	31.0	41.0
18	36.3097821939991	38.0	35.0	40.0	30.0	41.0
19	36.31215398180569	38.0	35.0	40.0	30.0	41.0
20	36.2810706322224	38.0	34.0	40.0	30.0	41.0
21	36.30625100679107	38.0	35.0	40.0	31.0	41.0
22	36.28003204774945	38.0	35.0	40.0	30.0	41.0
23	36.218015837353434	38.0	35.0	40.0	30.0	41.0
24	35.83230040102078	38.0	34.0	40.0	30.0	41.0
25	35.91032988834157	38.0	34.0	40.0	30.0	41.0
26	35.79333016812351	38.0	34.0	40.0	30.0	41.0
27	35.71493823601726	38.0	34.0	40.0	29.0	41.0
28	35.54445777412271	37.0	34.0	40.0	29.0	41.0
29	35.34095880422895	37.0	34.0	40.0	28.0	41.0
30	35.362735165198515	37.0	34.0	40.0	28.0	41.0
31	35.25255618953955	37.0	34.0	40.0	27.0	41.0
32	35.137989724372396	37.0	34.0	40.0	27.0	41.0
33	35.09918057804644	37.0	34.0	40.0	27.0	41.0
34	34.92478952767722	37.0	34.0	40.0	27.0	41.0
35	34.863123044705766	37.0	33.0	40.0	26.0	41.0
36	34.7691714215466	37.0	33.0	40.0	26.0	41.0
37	34.5477049402708	37.0	33.0	40.0	25.0	41.0
38	34.58338349625686	37.0	33.0	40.0	25.0	41.0
39	34.32486710357867	36.0	33.0	40.0	24.0	41.0
40	34.186273304563834	36.0	33.0	40.0	24.0	41.0
41	34.22631391533629	36.0	33.0	40.0	24.0	41.0
42	34.23203460817811	36.0	33.0	40.0	24.0	41.0
43	34.292868104011056	36.0	33.0	40.0	24.0	41.0
44	34.23248395492967	36.0	33.0	40.0	24.0	41.0
45	33.98906730875209	36.0	33.0	39.0	23.0	41.0
46	33.73689899871979	35.0	33.0	39.0	23.0	40.0
47	33.599676555121285	35.0	32.0	39.0	23.0	40.0
48	33.48299264936541	35.0	32.0	39.0	23.0	40.0
49	33.36148250515053	35.0	32.0	39.0	22.0	40.0
50	33.272338044409025	35.0	32.0	39.0	22.0	40.0
51	31.309205673638605	34.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	6.0
10	11.0
11	12.0
12	14.0
13	21.0
14	29.0
15	68.0
16	121.0
17	252.0
18	486.0
19	883.0
20	1426.0
21	2089.0
22	2844.0
23	3540.0
24	4202.0
25	4817.0
26	5789.0
27	7349.0
28	8695.0
29	11267.0
30	14601.0
31	18522.0
32	24425.0
33	32838.0
34	51368.0
35	53952.0
36	50466.0
37	61350.0
38	68120.0
39	42232.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.441885899838066	37.94627338934624	16.07389634503048	25.537944365785215
2	20.82128716648721	37.166275254559174	18.836742999092827	23.175694579860785
3	20.046587932072335	31.890902000016958	23.3577647966494	24.70474527126131
4	23.18883585278383	31.59416357917405	18.028342758310796	27.188657809731325
5	16.71951436637869	38.42720158712664	19.023476248208972	25.8298077982857
6	21.08792783321605	35.28855691866824	21.69327421173558	21.93024103638013
7	75.54472695826162	11.181951521420274	7.6130361427396585	5.660285377578445
8	78.26666610145062	7.1378307573612325	5.155618106130616	9.439885035057523
9	72.6667881881152	11.923373661497767	9.619199823652595	5.790638326734436
10	43.49231447490017	27.89235177915879	12.393916014548662	16.221417731392382
11	35.44582828171498	24.72827238891385	21.733969766594036	18.091929562777132
12	32.052624439376345	23.681633587397943	27.7359282401716	16.529813733054116
13	23.992361105223445	33.595876183774344	21.896328073998085	20.51543463700413
14	17.27123587313161	33.89070700048326	30.6558343012658	18.182222825119332
15	16.748552340418314	28.362894132209682	31.940923619530476	22.947629907841524
16	18.331651815615224	24.912250209836454	33.96446769366421	22.791630280884114
17	17.963060305725357	27.31625533069377	26.495137729018474	28.22554663456239
18	21.035574697538767	27.507227700107673	26.212176449143275	25.245021153210285
19	22.871961610526583	30.470160832224096	26.190768891639603	20.46710866560971
20	25.928367345208525	30.3919490627305	23.57374797582006	20.105935616240917
21	22.03791469194313	29.837260171769152	23.94869816615656	24.176126970131158
22	21.017346480258418	29.708814826747155	24.28316475764949	24.990673935344937
23	22.85988011767798	29.13738141060967	20.757276449991096	27.245462021721252
24	19.17735631501751	30.876480512763994	27.22447837624736	22.72168479597114
25	20.319375323232922	27.788917243893547	25.848459927595822	26.043247505277705
26	22.82914649551925	31.93286929096474	21.486405141205097	23.751579072310914
27	19.826789544633698	29.941966443123725	25.423064205716027	24.80817980652655
28	20.783558995837183	30.660921245623108	26.172964586389032	22.382555172150674
29	19.922169751333204	29.792749408642717	26.04769858159035	24.23738225843373
30	24.629924798005916	28.20901406540115	22.724652180179568	24.436408956413366
31	26.05214965790299	30.020602124647095	18.979813309142088	24.947434908307827
32	25.722346098737592	28.579513179425003	21.064188759548617	24.633951962288787
33	24.64518563107784	28.594985968511814	20.030267318925976	26.72956108148437
34	18.460944984696777	31.180213482098196	22.363691086825664	27.99515044637937
35	22.682049021187122	27.934742982136346	25.5833029529712	23.79990504370533
36	24.62208242545507	27.070174397409048	23.648992361105222	24.658750816030658
37	22.623549161078095	31.08207784720515	21.354568499944893	24.939804491771866
38	23.136482717106546	28.190785848120797	23.27425412678361	25.398477307989047
39	21.241172031979925	28.945137305106446	24.716614808095024	25.097075854818605
40	23.035167741990183	26.61086571314721	23.5781990521327	26.77576749272991
41	19.272312609687237	24.81708195915184	28.852724482615365	27.05788094854556
42	23.55445997846527	25.26091785432687	25.345064392237322	25.83955777497054
43	22.091115651679964	23.734410635105004	26.521844186894334	27.6526295263207
44	22.654282783236823	24.045774020975166	24.812206970809417	28.487736224978594
45	25.122722532620028	27.898286547575644	23.440851554485413	23.53813936531891
46	20.486184706949615	25.853758827968022	30.613867010318017	23.04618945476435
47	22.953564676258384	26.19712757208624	24.231235534001986	26.618072217653392
48	22.13753401894039	22.793113972988323	32.23745008435849	22.831901923712792
49	23.284428015498225	21.385090166088734	28.43813851749485	26.892343300918192
50	20.4880923110836	24.651332355509584	25.38173278281291	29.478842550593903
51	20.424505506617265	23.81071480046461	30.003221731426294	25.76155796149183
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3681.0
1	3064.0
2	2447.0
3	1754.0
4	1061.0
5	1012.0
6	963.0
7	997.5
8	1032.0
9	1084.0
10	1136.0
11	1208.5
12	1281.0
13	1365.5
14	1450.0
15	1559.5
16	1669.0
17	1703.5
18	1738.0
19	1647.0
20	1556.0
21	1525.0
22	1494.0
23	1797.5
24	2101.0
25	1866.0
26	1870.0
27	2109.0
28	2577.0
29	3045.0
30	3544.0
31	4043.0
32	5111.0
33	6179.0
34	7208.5
35	8238.0
36	8533.0
37	8828.0
38	10109.5
39	11391.0
40	12963.0
41	14535.0
42	20891.5
43	27248.0
44	38967.5
45	50687.0
46	49957.0
47	49227.0
48	47064.0
49	44901.0
50	42418.0
51	39935.0
52	36610.5
53	33286.0
54	30953.5
55	28621.0
56	24851.5
57	21082.0
58	20222.0
59	19362.0
60	18859.5
61	18357.0
62	16514.0
63	14671.0
64	13294.5
65	11918.0
66	10200.5
67	8483.0
68	7662.0
69	6841.0
70	6076.5
71	5312.0
72	4342.5
73	3373.0
74	2974.0
75	2201.5
76	1828.0
77	1414.5
78	1001.0
79	853.0
80	705.0
81	586.0
82	467.0
83	308.5
84	150.0
85	109.0
86	68.0
87	53.0
88	38.0
89	28.5
90	19.0
91	17.0
92	15.0
93	14.5
94	14.0
95	8.0
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	471796.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.456386105392873
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.41275699659892	23.39212831785557
2	9.045906893848668	5.329194522772831
3	3.2700485403408517	2.889714371629695
4	1.4712541489499382	1.7335132108252234
5	0.855172606509211	1.2595147242045266
6	0.5523642501458444	0.9762392773867083
7	0.3560679453260993	0.7341932414095652
8	0.2856395407316509	0.6731126879006882
9	0.2283440671560779	0.6053571906322673
>10	3.1111305612823124	22.585881563463555
>50	1.1938545956265079	24.397151422862816
>100	0.2058619946314075	8.211101285795912
>500	0.005074063247957228	1.0112277847287665
>1k	0.004349197069677624	2.4452239422959954
>5k	0.002174598534838812	3.756446456235893
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCC	6136	1.3005621073514824	No Hit
AATCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCT	5736	1.2157797013963663	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGT	5721	1.2126003611730494	No Hit
CTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGCT	3251	0.6890690044002069	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3125	0.6623625465243452	No Hit
AATGACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTT	1448	0.30691230955752064	No Hit
AAACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCT	1387	0.2939829926493654	No Hit
AATGATCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCT	1194	0.25307548177602185	No Hit
AAAAACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTT	1047	0.22191794758751665	No Hit
AACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTG	975	0.20665711451559574	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	798	0.1691408998804568	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCACGCACATTCGTAT	679	0.14391813410880974	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	606	0.12844534502200106	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCT	575	0.12187470856047952	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	569	0.1206029724711528	No Hit
CGTAGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	534	0.11318451195008013	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9109869519877235	0.0
2	0.0	0.0	0.0	1.1642743897786332	0.0
3	0.0	0.0	0.0	1.7414306183180865	0.0
4	0.0	0.0	0.0	4.782363563913217	0.0
5	0.0	0.0	0.0	5.1524387659072985	0.0
6	0.0	0.0	0.0	6.862923806051768	0.0
7	0.0	0.0	0.0	8.147801168301553	0.0
8	0.0	0.0	0.0	8.69697920287582	0.0
9	0.0	0.0	0.0	10.92336518325717	0.0
10	0.0	0.0	0.0	12.302351016117136	0.0
11	0.0	0.0	0.0	15.30152862677937	0.0
12	0.0	0.0	0.0	16.058211599928782	0.0
13	0.0	0.0	0.0	16.42192812147623	0.0
14	0.0	0.0	0.0	16.889503090318698	0.0
15	0.0	0.0	0.0	17.20468168445684	0.0
16	0.0	0.0	0.0	17.689637046520104	0.0
17	0.0	0.0	0.0	18.26870087919355	0.0
18	0.0	0.0	0.0	19.10062823762813	0.0
19	0.0	0.0	0.0	19.45162739828231	0.0
20	0.0	0.0	0.0	19.80283851495138	0.0
21	0.0	0.0	0.0	20.171641980856133	0.0
22	0.0	0.0	0.0	20.54786390728196	0.0
23	0.0	0.0	0.0	20.931504294228862	0.0
24	0.0	0.0	0.0	21.22315577071446	0.0
25	0.0	0.0	0.0	21.443166114167987	0.0
26	0.0	0.0	0.0	21.66847535799371	0.0
27	0.0	0.0	0.0	21.89865959016185	0.0
28	0.0	0.0	0.0	22.123332965942907	0.0
29	0.0	0.0	0.0	22.364114998855438	0.0
30	2.1195601488779048E-4	0.0	0.0	22.705788094854555	0.0
31	2.1195601488779048E-4	0.0	0.0	22.967765729255866	0.0
32	2.1195601488779048E-4	0.0	0.0	23.21003145427261	0.0
33	2.1195601488779048E-4	0.0	0.0	23.462471068003968	0.0
34	2.1195601488779048E-4	0.0	0.0	23.702405276856947	0.0
35	2.1195601488779048E-4	0.0	0.0	23.995328489431873	0.0
36	2.1195601488779048E-4	0.0	0.0	24.23632247835929	0.0
37	2.1195601488779048E-4	0.0	0.0	24.485582751867334	0.0
38	2.1195601488779048E-4	0.0	0.0	24.735690849434924	0.0
39	2.1195601488779048E-4	0.0	0.0	24.986434815047183	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGTC	20	7.029052E-4	45.000004	44
TCGCACA	20	7.029052E-4	45.000004	24
CAAACCG	20	7.029052E-4	45.000004	1
AGATCAT	20	7.029052E-4	45.000004	31
CGAACAG	20	7.029052E-4	45.000004	1
GCGAATT	20	7.029052E-4	45.000004	9
CCTAGCG	20	7.029052E-4	45.000004	1
TAGACGT	30	2.1627711E-6	45.000004	18
TAGCGTG	20	7.029052E-4	45.000004	19
CGTTGAA	20	7.029052E-4	45.000004	20
CGTCTAC	20	7.029052E-4	45.000004	42
GATCAAT	20	7.029052E-4	45.000004	9
TACGGTG	20	7.029052E-4	45.000004	45
GTCTAAG	20	7.029052E-4	45.000004	23
TGCGGTT	20	7.029052E-4	45.000004	35
ACGGGTA	40	6.8012014E-9	45.000004	5
CACCTCG	20	7.029052E-4	45.000004	37
TCGTGTT	20	7.029052E-4	45.000004	42
GTAGCGC	20	7.029052E-4	45.000004	18
GCACAAT	40	6.8012014E-9	45.000004	35
>>END_MODULE