FastQCFastQC Report
Sat 18 Jun 2016
SRR3552984_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552984_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398097
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCC131153.2944232184618323No Hit
AATCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCT113942.8621165193407636No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGT108782.732499868122593No Hit
CTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGCT53211.3366089169222577TruSeq Adapter, Index 16 (95% over 22bp)
AATGACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTT27530.6915400015574094No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22730.5709663725172508No Hit
AATGATCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCT20390.5121867283601735No Hit
AACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTG15650.393120269683017No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTAT14410.361972082180976No Hit
AAACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCT10310.25898210737584054No Hit
AATGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTC8860.22255882360329268No Hit
AAAAACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTT7130.1791020781367355No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGGATCTGTCGTATGCC5720.14368357460618894No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5250.13187740676267343No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATG4930.12383916482666285No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTATCTGTC4450.11178180192264699No Hit
AATGATCCGGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCC4130.10374355998663642No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATG302.1619126E-645.0000041
TAACCCG302.1619126E-645.0000041
ATCCCGG302.1619126E-645.0000042
TAGGGCG302.1619126E-645.0000045
GCGGCTA600.045.00000423
CGTAATG302.1619126E-645.0000041
AAGGGTC302.1619126E-645.0000045
ATCGCGG302.1619126E-645.0000042
TGATCGT207.027726E-445.028
TCGCCTT207.027726E-445.039
CCTTCCG207.027726E-445.01
CTTAACG351.209246E-745.01
AAGTAGT207.027726E-445.023
AGACTCG207.027726E-445.027
CAGCGCG207.027726E-445.01
TCGTTGA453.8380676E-1045.024
TTGGCGG253.8861963E-545.02
ATCTCGT207.027726E-445.014
CTCACGG700.045.02
GGAGCGT207.027726E-445.035