##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552984_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	398097
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.00620954189557	31.0	31.0	33.0	28.0	34.0
2	31.209875984998632	31.0	31.0	34.0	28.0	34.0
3	31.126662597306687	31.0	31.0	34.0	28.0	34.0
4	35.112824261423725	35.0	35.0	37.0	32.0	37.0
5	34.74526560109722	35.0	35.0	37.0	32.0	37.0
6	34.81430656347574	35.0	35.0	37.0	32.0	37.0
7	34.70109797360945	35.0	35.0	37.0	32.0	37.0
8	35.11532867617691	37.0	35.0	37.0	32.0	37.0
9	35.77795110236952	39.0	35.0	39.0	32.0	39.0
10	35.48335707126655	37.0	35.0	39.0	30.0	39.0
11	35.91712070173852	37.0	35.0	39.0	31.0	39.0
12	35.54544244241981	37.0	35.0	39.0	30.0	39.0
13	35.477574560973835	37.0	35.0	39.0	30.0	39.0
14	36.35797305681781	38.0	35.0	40.0	31.0	41.0
15	36.533781967711384	38.0	35.0	40.0	31.0	41.0
16	36.40578301268284	38.0	35.0	40.0	31.0	41.0
17	36.3695154698478	38.0	35.0	40.0	31.0	41.0
18	36.10813696159479	38.0	35.0	40.0	30.0	41.0
19	36.13572822704015	38.0	34.0	40.0	30.0	41.0
20	36.265671431836964	38.0	34.0	40.0	30.0	41.0
21	36.42858147637385	38.0	35.0	40.0	31.0	41.0
22	36.42537622740186	38.0	35.0	40.0	31.0	41.0
23	36.400892244854894	38.0	35.0	40.0	31.0	41.0
24	35.9828810566269	38.0	34.0	40.0	30.0	41.0
25	36.10040266568198	38.0	34.0	40.0	30.0	41.0
26	35.991662835941995	38.0	34.0	40.0	30.0	41.0
27	35.872493387290035	38.0	34.0	40.0	30.0	41.0
28	35.82296023330997	38.0	34.0	40.0	30.0	41.0
29	35.67443361793734	38.0	34.0	40.0	29.0	41.0
30	35.50527886419641	38.0	34.0	40.0	29.0	41.0
31	35.368352436717686	37.0	34.0	40.0	28.0	41.0
32	35.43296985408079	38.0	34.0	40.0	29.0	41.0
33	35.442550433688275	38.0	34.0	40.0	29.0	41.0
34	35.58510363052221	38.0	34.0	40.0	29.0	41.0
35	35.238238419279725	38.0	34.0	40.0	27.0	41.0
36	35.14915711497449	37.0	34.0	40.0	27.0	41.0
37	34.77501714406288	37.0	33.0	40.0	25.0	41.0
38	34.87715054371171	37.0	33.0	40.0	26.0	41.0
39	34.94299630492066	37.0	33.0	40.0	26.0	41.0
40	34.76542149275177	37.0	33.0	40.0	26.0	41.0
41	34.97975870202488	37.0	34.0	40.0	26.0	41.0
42	34.947811714230454	37.0	34.0	40.0	26.0	41.0
43	34.881435931443846	37.0	33.0	40.0	26.0	41.0
44	34.773238683034535	37.0	33.0	40.0	26.0	41.0
45	34.43933011301266	37.0	33.0	40.0	25.0	41.0
46	34.16645691879115	37.0	33.0	40.0	24.0	41.0
47	33.99268519983823	36.0	33.0	40.0	23.0	40.0
48	33.98723929092658	36.0	33.0	40.0	23.0	41.0
49	33.86434964342861	36.0	32.0	39.0	23.0	40.0
50	33.699475253518614	36.0	32.0	39.0	23.0	40.0
51	31.651172955335007	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	2.0
11	3.0
12	4.0
13	7.0
14	3.0
15	25.0
16	58.0
17	133.0
18	264.0
19	475.0
20	867.0
21	1258.0
22	1753.0
23	2363.0
24	2996.0
25	3571.0
26	4512.0
27	5812.0
28	7249.0
29	9379.0
30	12633.0
31	16129.0
32	21286.0
33	27844.0
34	41408.0
35	45465.0
36	43231.0
37	52701.0
38	60287.0
39	36374.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.038309758676906	43.03649613034009	17.632386076760188	22.292808034222816
2	17.66780458029073	42.5079817230474	19.692185572862893	20.132028123798975
3	18.492477963913316	28.2117172447921	31.99496605098757	21.30083874030701
4	26.6892239830996	28.318977535625738	18.604762155956966	26.3870363253177
5	15.46155836391633	40.91741459995931	19.782364599582515	23.838662436541846
6	21.583181988309384	31.257959743479603	27.837687799707105	19.321170468503908
7	69.53606784276194	14.375893312433904	9.503713918969497	6.584324925834658
8	72.42556462369724	4.76064878660226	6.228632720166191	16.58515386953431
9	69.69582790124015	10.906387136803342	12.106345940813421	7.291439021143089
10	36.12310567525	34.22959730919851	14.018693936402435	15.62860307914905
11	28.107973684805458	20.233008538120107	31.625960507112588	20.033057269961844
12	25.912277660972077	22.156660311431637	37.12562516170682	14.805436865889469
13	22.375953599248426	34.41271850830325	23.49050608268839	19.720821809759933
14	13.39271584563561	32.8548067430802	37.85233247173427	15.900144939549909
15	12.63837707895312	30.017809729789473	31.246655965757093	26.097157225500318
16	14.791118747440951	26.71509707433113	37.306485605267056	21.18729857296086
17	14.141025930866096	27.40588349070704	30.308944804909355	28.144145773517508
18	17.023238055046885	27.72766436320796	28.804537587572877	26.444559994172273
19	17.865997483025495	31.23761294357908	33.11353765539554	17.782851917999885
20	21.99087157150142	33.20170712163116	26.18180996088893	18.625611345978495
21	16.46558502073615	28.580471593606582	28.026335290142857	26.92760809551441
22	17.11768739779501	32.41270343659962	28.375998814359317	22.093610351246053
23	20.56081809207304	28.06954084054891	22.178765476755665	29.190875590622383
24	14.695915819511324	37.015099335086674	26.98136383846148	21.30762100694052
25	18.109907886771314	25.81632114786095	28.919584925282027	27.15418604008571
26	23.727885414861202	33.55991127790463	21.09460759563624	21.61759571159793
27	14.883809724765573	28.882910446448978	32.21903204495386	24.01424778383158
28	24.474185939607686	27.302139930720404	26.455863771894787	21.767810357777126
29	21.341783535168567	27.889433982170175	31.961808303001533	18.80697417965973
30	22.93787694958766	24.98787983833086	28.791726639487365	23.282516572594115
31	26.631197924124024	34.14067425778139	21.89139832754329	17.3367294905513
32	22.955962993943686	23.96827908776002	27.859541769970637	25.21621614832566
33	27.091136079900124	25.859275503206504	23.686187034818147	23.36340138207522
34	21.511089005945788	24.54025024051927	29.20494251401041	24.743718239524537
35	28.100437832990455	22.726873098767385	29.032622702507176	20.140066365734985
36	25.577434645325138	25.766082135760882	31.11101063308691	17.54547258582707
37	20.454813776541897	25.666860086863252	32.191149393238334	21.687176743356517
38	22.706777493927362	29.60635222068993	26.370457451324675	21.31641283405803
39	18.86675860405881	23.76581586899675	36.89603287640952	20.47139265053492
40	25.005463492565884	26.348352286000647	22.891405863395104	25.754778358038365
41	19.2400344639623	20.413366591559342	37.6445941567005	22.702004787777852
42	31.30141648894616	22.646993069528282	23.160184578130455	22.891405863395104
43	23.732406925950208	22.37369284370392	29.20921283003891	24.68468740030696
44	20.16669304214802	23.03659660836429	27.889936372291174	28.906773977196515
45	27.905510466042195	28.54907221104404	23.661067528768115	19.88434979414565
46	18.08478838072128	22.98962313205073	38.10779784826311	20.817790638964876
47	22.284267402165803	26.4641532088913	24.66283343004343	26.588745958899462
48	20.823568125356385	19.3148905919914	38.659171006061335	21.20237027659088
49	24.467403672974175	18.992356134308974	31.633496358927598	24.906743833789253
50	20.01446883548482	22.201373032200696	25.599791005709665	32.184367126604826
51	17.509049302054525	22.03759385275448	35.56545264093927	24.887904204251726
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2600.0
1	2031.0
2	1462.0
3	1015.0
4	568.0
5	503.0
6	438.0
7	477.0
8	516.0
9	538.5
10	561.0
11	596.5
12	632.0
13	738.5
14	845.0
15	865.5
16	886.0
17	874.5
18	863.0
19	947.5
20	1032.0
21	1069.0
22	1106.0
23	1189.5
24	1273.0
25	1842.5
26	2606.5
27	2801.0
28	3157.5
29	3514.0
30	4558.0
31	5602.0
32	6008.0
33	6414.0
34	7880.5
35	9347.0
36	10458.5
37	11570.0
38	12745.0
39	13920.0
40	15487.5
41	17055.0
42	21874.0
43	26693.0
44	46034.0
45	65375.0
46	62021.5
47	58668.0
48	51811.5
49	44955.0
50	39440.0
51	33925.0
52	30082.5
53	26240.0
54	22605.5
55	18971.0
56	15557.0
57	12143.0
58	10501.5
59	8860.0
60	7647.5
61	6435.0
62	5463.0
63	4491.0
64	3453.5
65	2416.0
66	1826.0
67	1236.0
68	1071.5
69	907.0
70	657.0
71	407.0
72	352.0
73	297.0
74	295.5
75	265.5
76	237.0
77	157.0
78	77.0
79	50.5
80	24.0
81	18.5
82	13.0
83	9.0
84	5.0
85	4.5
86	4.0
87	4.5
88	5.0
89	2.5
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	398097.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.73853629316568
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.95030505439311	25.057671223253575
2	9.480107012073438	6.017694409315745
3	3.2599156162268086	3.103948502948164
4	1.4851111221244446	1.885410129957228
5	0.8426076171889838	1.337156621952521
6	0.5699670107817318	1.0853951194561888
7	0.43943685480777345	0.9762957795409768
8	0.3880425505578886	0.9852722059339301
9	0.2876690381981375	0.8217174788344486
>10	3.527090106616256	26.32367769624122
>50	0.6820251937352733	14.349384772703552
>100	0.07665868342099827	4.384729324773297
>500	0.0031611828214844644	0.6762330678787718
>1k	0.004741774232226697	2.784695667503755
>5k	7.902957053711161E-4	1.334657327219193
>10k+	0.0023708871161133484	8.876060672487423
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCC	13115	3.2944232184618323	No Hit
AATCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCT	11394	2.8621165193407636	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGT	10878	2.732499868122593	No Hit
CTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGCT	5321	1.3366089169222577	TruSeq Adapter, Index 16 (95% over 22bp)
AATGACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTT	2753	0.6915400015574094	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2273	0.5709663725172508	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCT	2039	0.5121867283601735	No Hit
AACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTG	1565	0.393120269683017	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTAT	1441	0.361972082180976	No Hit
AAACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCT	1031	0.25898210737584054	No Hit
AATGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTC	886	0.22255882360329268	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTT	713	0.1791020781367355	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGGATCTGTCGTATGCC	572	0.14368357460618894	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	525	0.13187740676267343	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATG	493	0.12383916482666285	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTATCTGTC	445	0.11178180192264699	No Hit
AATGATCCGGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCC	413	0.10374355998663642	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.7694180061643268	0.0
2	0.0	0.0	0.0	2.0879333428787454	0.0
3	0.0	0.0	0.0	2.968874420053404	0.0
4	0.0	0.0	0.0	8.206542626545792	0.0
5	0.0	0.0	0.0	8.743095275774497	0.0
6	0.0	0.0	0.0	10.925477961401366	0.0
7	0.0	0.0	0.0	12.6235565703836	0.0
8	0.0	0.0	0.0	13.232453397036402	0.0
9	0.0	0.0	0.0	17.56029309439659	0.0
10	0.0	0.0	0.0	19.199592059221747	0.0
11	0.0	0.0	0.0	24.85977035747569	0.0
12	0.0	0.0	0.0	25.880124693228034	0.0
13	0.0	0.0	0.0	26.439033702841268	0.0
14	0.0	0.0	0.0	27.253910479104338	0.0
15	0.0	0.0	0.0	27.674159815321392	0.0
16	0.0	0.0	0.0	28.361931890971295	0.0
17	0.0	0.0	0.0	29.191377980743386	0.0
18	0.0	0.0	0.0	30.396863076084472	0.0
19	0.0	0.0	0.0	30.884181493455113	0.0
20	0.0	0.0	0.0	31.31573460739468	0.0
21	0.0	0.0	0.0	31.857562352893893	0.0
22	0.0	0.0	0.0	32.42476079950364	0.0
23	0.0	0.0	0.0	32.999746292988895	0.0
24	0.0	0.0	0.0	33.39487612315592	0.0
25	0.0	0.0	0.0	33.72469523759285	0.0
26	0.0	0.0	0.0	34.06255259396579	0.0
27	0.0	0.0	0.0	34.383328686224715	0.0
28	0.0	0.0	0.0	34.686521124248614	0.0
29	0.0	0.0	0.0	35.036435843525574	0.0
30	0.0	0.0	0.0	35.516720799202204	0.0
31	0.0	0.0	0.0	35.8897454640452	0.0
32	0.0	0.0	0.0	36.1936914872506	0.0
33	0.0	0.0	0.0	36.500651851182	0.0
34	0.0	0.0	0.0	36.84076996309945	0.0
35	0.0	0.0	0.0	37.22057689457594	0.0
36	0.0	0.0	0.0	37.55516871516239	0.0
37	0.0	0.0	0.0	37.88574141478082	0.0
38	0.0	0.0	0.0	38.214555748975755	0.0
39	0.0	0.0	0.0	38.54638442389669	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCATG	30	2.1619126E-6	45.000004	1
TAACCCG	30	2.1619126E-6	45.000004	1
ATCCCGG	30	2.1619126E-6	45.000004	2
TAGGGCG	30	2.1619126E-6	45.000004	5
GCGGCTA	60	0.0	45.000004	23
CGTAATG	30	2.1619126E-6	45.000004	1
AAGGGTC	30	2.1619126E-6	45.000004	5
ATCGCGG	30	2.1619126E-6	45.000004	2
TGATCGT	20	7.027726E-4	45.0	28
TCGCCTT	20	7.027726E-4	45.0	39
CCTTCCG	20	7.027726E-4	45.0	1
CTTAACG	35	1.209246E-7	45.0	1
AAGTAGT	20	7.027726E-4	45.0	23
AGACTCG	20	7.027726E-4	45.0	27
CAGCGCG	20	7.027726E-4	45.0	1
TCGTTGA	45	3.8380676E-10	45.0	24
TTGGCGG	25	3.8861963E-5	45.0	2
ATCTCGT	20	7.027726E-4	45.0	14
CTCACGG	70	0.0	45.0	2
GGAGCGT	20	7.027726E-4	45.0	35
>>END_MODULE