FastQCFastQC Report
Sat 18 Jun 2016
SRR3552981_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552981_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477697
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGCT48271.0104731660445847TruSeq Adapter, Index 13 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27140.5681425673596443No Hit
AAACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCT14310.2995622748311168No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCC10750.22503804713029388No Hit
AATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCT9660.20222023583987336No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTT9500.19887083234770156No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGT9190.19238136308161868No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6750.1413029598259985No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA6300.13188276250426526No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG5750.12036918799992465No Hit
CCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC5450.11408905645210249TruSeq Adapter, Index 16 (95% over 21bp)
AAAAAACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCT4930.10320349510254408No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGG207.029141E-445.0000042
GTCGCTC207.029141E-445.00000413
CGAACAC207.029141E-445.00000414
CTACTGA207.029141E-445.00000429
ACGTTGT207.029141E-445.00000439
CAATTAG207.029141E-445.0000041
CGTTCCG207.029141E-445.0000041
TTATCCG351.2099372E-745.0000041
ATCCGTG207.029141E-445.00000426
TATTGGT207.029141E-445.00000427
TCCAGTA207.029141E-445.00000432
GTTCGGA207.029141E-445.00000415
GTATGCG207.029141E-445.0000041
CGTAGCG406.8012014E-945.0000041
TGCGAAA207.029141E-445.00000429
ACTACCG351.2099372E-745.0000041
GATATCG207.029141E-445.00000440
GTCGTAC207.029141E-445.00000422
TGTTACG406.8012014E-945.0000041
TTGCGAA207.029141E-445.00000415