##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3552981_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 477697 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.38523164265214 31.0 31.0 34.0 30.0 34.0 2 31.57049133655853 31.0 31.0 34.0 30.0 34.0 3 31.774551650941916 31.0 31.0 34.0 30.0 34.0 4 35.61371120187064 37.0 35.0 37.0 33.0 37.0 5 35.215804160377814 37.0 35.0 37.0 33.0 37.0 6 35.22810903145718 37.0 35.0 37.0 32.0 37.0 7 35.2338720988409 37.0 35.0 37.0 32.0 37.0 8 35.53762112803723 37.0 35.0 37.0 33.0 37.0 9 36.3796758196095 39.0 37.0 39.0 32.0 39.0 10 36.03902473743817 38.0 35.0 39.0 31.0 39.0 11 36.27438313407872 38.0 35.0 39.0 32.0 39.0 12 35.854702876509585 37.0 35.0 39.0 31.0 39.0 13 35.528167436680576 37.0 35.0 39.0 30.0 39.0 14 36.577384827620854 38.0 35.0 40.0 31.0 41.0 15 36.61864738526723 38.0 35.0 40.0 31.0 41.0 16 36.67658578555025 38.0 35.0 40.0 31.0 41.0 17 36.653447687550894 38.0 35.0 40.0 31.0 41.0 18 36.50774863564142 38.0 35.0 40.0 31.0 41.0 19 36.54767352526811 38.0 35.0 40.0 31.0 41.0 20 36.48732774122509 38.0 35.0 40.0 31.0 41.0 21 36.48174889103344 38.0 35.0 40.0 31.0 41.0 22 36.48441166680971 38.0 35.0 40.0 31.0 41.0 23 36.39446343602744 38.0 35.0 40.0 31.0 41.0 24 36.06415782389255 38.0 34.0 40.0 30.0 41.0 25 36.18256133071801 38.0 35.0 40.0 30.0 41.0 26 36.0611915084248 38.0 35.0 40.0 30.0 41.0 27 35.919865521449786 38.0 34.0 40.0 30.0 41.0 28 35.952352641946675 38.0 34.0 40.0 30.0 41.0 29 35.65918354103124 38.0 34.0 40.0 29.0 41.0 30 35.637571514998 38.0 34.0 40.0 29.0 41.0 31 35.51386757714618 38.0 34.0 40.0 29.0 41.0 32 35.454049324153175 38.0 34.0 40.0 29.0 41.0 33 35.4360651207774 38.0 34.0 40.0 29.0 41.0 34 35.26225829343705 37.0 34.0 40.0 27.0 41.0 35 35.19837470195542 37.0 34.0 40.0 27.0 41.0 36 35.0061147547504 37.0 34.0 40.0 27.0 41.0 37 34.84212586639648 37.0 33.0 40.0 26.0 41.0 38 34.78332290133704 37.0 33.0 40.0 26.0 41.0 39 34.75235138592036 37.0 33.0 40.0 26.0 41.0 40 34.60191920820102 37.0 33.0 40.0 25.0 41.0 41 34.71145098252658 37.0 34.0 40.0 26.0 41.0 42 34.58130990983825 37.0 33.0 40.0 26.0 41.0 43 34.446904627828935 36.0 33.0 40.0 25.0 41.0 44 34.245659905756156 36.0 33.0 40.0 24.0 41.0 45 33.96939691896746 35.0 33.0 40.0 23.0 41.0 46 33.912333550346766 35.0 33.0 39.0 23.0 41.0 47 33.77973275946887 35.0 33.0 39.0 23.0 41.0 48 33.79264680330837 35.0 33.0 39.0 23.0 41.0 49 33.605699847392806 35.0 33.0 39.0 23.0 40.0 50 33.34837355059798 35.0 32.0 39.0 22.0 40.0 51 31.447057444363267 34.0 29.0 38.0 17.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 4.0 10 7.0 11 10.0 12 11.0 13 9.0 14 30.0 15 69.0 16 105.0 17 228.0 18 443.0 19 815.0 20 1289.0 21 1926.0 22 2582.0 23 3514.0 24 4185.0 25 4908.0 26 5790.0 27 7074.0 28 8469.0 29 10476.0 30 13241.0 31 17097.0 32 22163.0 33 30445.0 34 50442.0 35 53089.0 36 46505.0 37 62202.0 38 76514.0 39 54054.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 19.970190308919673 32.13103703812249 21.040324724668565 26.85844792828927 2 23.763808439240776 31.73915682953839 20.989664996849466 23.50736973437137 3 21.38426659577096 31.717176369121013 19.399326351222637 27.49923068388539 4 19.577472749462526 33.35775606713042 19.416701381838276 27.648069801568774 5 18.29988465491724 35.45825073215867 18.64926930669441 27.592595306229683 6 20.450620372328064 37.71093391836248 19.409793237135673 22.428652472173784 7 80.77170256459638 7.873819596941158 6.072259193589242 5.282218644873215 8 83.29380339420176 6.207491359585679 5.199948921596744 5.298756324615813 9 76.0475782766063 10.686271841774179 8.706774377900636 4.559375503718885 10 44.34317150829919 28.678639388566392 11.506875697356273 15.471313405778139 11 35.97698959800878 25.46593342641884 21.777821506101148 16.77925546947123 12 34.05903742330389 25.206982668930305 24.129102757605764 16.60487715016004 13 24.782236438579268 33.282185150838295 21.03509128171205 20.90048712887039 14 18.162140436301673 34.37618406646891 27.233162443975996 20.228513053253423 15 17.2854340722257 28.625258270409905 32.68368861433084 21.405619043033557 16 19.173241615501038 25.650778631643075 32.58195048325612 22.594029269599766 17 19.044289581052425 26.414861303294767 27.20866993093949 27.332179184713322 18 22.045355110038372 26.720913047391964 26.595101078717263 24.6386307638524 19 24.00705886785975 30.681164001448618 24.48162747515685 20.830149655534786 20 27.53272471880711 28.565597020705596 23.299916055574975 20.60176220491232 21 21.52033611264044 31.967544280160855 23.503182980006155 23.00893662719255 22 22.378202082072946 27.496927968984526 24.21974598961266 25.905123959329867 23 22.829743540361356 30.80571994381376 21.83497070318633 24.529565812638555 24 20.383841640202892 27.99996650596508 28.138338737735424 23.477853116096608 25 21.365844876564015 27.264563101715105 25.8756073410551 25.493984680665775 26 22.812159172027457 30.793159680718112 22.554464440848488 23.840216706405943 27 19.53267447775473 30.513903164558286 25.5130344130275 24.44038794465948 28 22.446236840507687 28.208885548789297 27.984475514813784 21.360402095889235 29 20.462133946832406 28.549059340962994 27.7071030381183 23.281703674086295 30 23.3509944588306 27.420938377255876 26.04914830949326 23.17891885442027 31 29.330935718666858 26.023818445583707 21.269968201600598 23.375277634148844 32 26.517646122960787 24.976083165688713 25.558879373326604 22.94739133802389 33 26.405441105973033 25.11801413866949 23.81656154424248 24.659983211114994 34 21.09747392175375 25.360636554133688 28.42848081524481 25.11340870886776 35 23.425518686531422 24.188554669591813 28.628817011620338 23.75710963225643 36 25.98530030542373 25.795640332679504 27.038897041430026 21.18016232046674 37 22.551115037356315 28.291783285220546 26.64345809163549 22.513643585787644 38 23.861569153668537 28.40566300395439 26.112368300408 21.620399541969075 39 22.474916107909408 25.779521328373423 27.103163720936074 24.642398842781095 40 24.079908393814488 24.328601603108247 25.158206980575553 26.433283022501712 41 20.52849400352106 21.427599503450935 30.039125219542935 28.004781273485076 42 24.599275272819384 23.471572984548782 25.126806322836444 26.802345419795394 43 22.215337337266092 24.265590949911765 26.46614904426865 27.052922668553496 44 22.95492749588128 23.92102106565459 25.85195217889164 27.272099259572492 45 24.89360410469398 26.306005689799182 24.85131788560531 23.949072319901525 46 21.453766718233524 26.163656041381877 29.32130618362686 23.061271056757736 47 21.959107970114946 25.065470371386045 26.768642047155417 26.206779611343595 48 23.679026663345176 20.80419177847045 32.11031260401468 23.406468954169693 49 23.075506021599466 22.29404831933213 30.23799605189063 24.39244960717777 50 21.22789132023019 24.658308509368908 26.559723004331197 27.554077166069707 51 19.852542511257134 23.871198688708535 30.439169599139206 25.83708920089513 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3151.0 1 2510.0 2 1869.0 3 1298.5 4 728.0 5 676.5 6 625.0 7 637.5 8 650.0 9 782.0 10 914.0 11 905.0 12 896.0 13 948.0 14 1000.0 15 1091.5 16 1183.0 17 1372.0 18 1561.0 19 1515.5 20 1470.0 21 1476.5 22 1483.0 23 1498.0 24 1513.0 25 1606.5 26 2120.0 27 2540.0 28 2797.5 29 3055.0 30 4049.5 31 5044.0 32 5561.5 33 6079.0 34 7080.5 35 8082.0 36 9266.5 37 10451.0 38 11252.0 39 12053.0 40 14904.5 41 17756.0 42 24597.5 43 31439.0 44 36002.0 45 40565.0 46 42021.0 47 43477.0 48 45162.0 49 46847.0 50 44167.0 51 41487.0 52 38735.5 53 35984.0 54 32906.5 55 29829.0 56 27726.5 57 25624.0 58 23647.0 59 21670.0 60 20844.5 61 20019.0 62 18706.0 63 17393.0 64 15117.0 65 12841.0 66 10680.5 67 8520.0 68 7383.5 69 6247.0 70 5509.0 71 4771.0 72 3783.0 73 2795.0 74 2406.0 75 1600.5 76 1184.0 77 882.0 78 580.0 79 414.0 80 248.0 81 203.0 82 158.0 83 126.0 84 94.0 85 75.5 86 57.0 87 42.0 88 27.0 89 14.5 90 2.0 91 4.5 92 7.0 93 6.5 94 6.0 95 4.5 96 3.0 97 2.5 98 2.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 477697.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.729098227975047 #Duplication Level Percentage of deduplicated Percentage of total 1 79.09714981797666 22.7238978667351 2 9.193923490353702 5.282662621097174 3 3.144934347124824 2.710533833372449 4 1.4390074595152629 1.6536554662081124 5 0.813299338726244 1.1682678295506708 6 0.4851126013603227 0.8362108545665526 7 0.3637074256005402 0.7314290449425291 8 0.2870955448038719 0.6598396885987557 9 0.2389814307573021 0.6179148898999693 >10 3.3324793740084617 23.96113707357378 >50 1.3697099178805225 27.755101498810188 >100 0.2265849917344907 8.695420744521739 >500 0.005099983736789179 1.1009776163537623 >1k 0.002914276421022388 2.102950971769249 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGCT 4827 1.0104731660445847 TruSeq Adapter, Index 13 (95% over 22bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2714 0.5681425673596443 No Hit AAACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCT 1431 0.2995622748311168 No Hit AATGATACGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCC 1075 0.22503804713029388 No Hit AATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCT 966 0.20222023583987336 No Hit AAAAACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTT 950 0.19887083234770156 No Hit AATGATACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGT 919 0.19238136308161868 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 675 0.1413029598259985 No Hit ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA 630 0.13188276250426526 No Hit TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG 575 0.12036918799992465 No Hit CCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC 545 0.11408905645210249 TruSeq Adapter, Index 16 (95% over 21bp) AAAAAACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCT 493 0.10320349510254408 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.093377182607385E-4 0.0 0.0 1.3588111292304537 0.0 2 2.093377182607385E-4 0.0 0.0 1.681191215351991 0.0 3 2.093377182607385E-4 0.0 0.0 2.054231029292627 0.0 4 2.093377182607385E-4 0.0 0.0 4.01949352832444 0.0 5 2.093377182607385E-4 0.0 0.0 4.26044124204255 0.0 6 2.093377182607385E-4 0.0 0.0 5.60962283623301 0.0 7 2.093377182607385E-4 0.0 0.0 6.681641291446251 0.0 8 2.093377182607385E-4 0.0 0.0 7.195146714339843 0.0 9 2.093377182607385E-4 0.0 0.0 8.375392769893887 0.0 10 2.093377182607385E-4 0.0 0.0 9.786328990971263 0.0 11 2.093377182607385E-4 0.0 0.0 11.56842098652493 0.0 12 2.093377182607385E-4 0.0 0.0 12.303824390774906 0.0 13 2.093377182607385E-4 0.0 0.0 12.568427266656478 0.0 14 2.093377182607385E-4 0.0 0.0 12.903576953591921 0.0 15 2.093377182607385E-4 0.0 0.0 13.153107513758721 0.0 16 2.093377182607385E-4 0.0 0.0 13.755372129194866 0.0 17 2.093377182607385E-4 0.0 0.0 14.417507332053582 0.0 18 2.093377182607385E-4 0.0 0.0 15.242507279719153 0.0 19 2.093377182607385E-4 0.0 0.0 15.62203656292587 2.093377182607385E-4 20 2.093377182607385E-4 0.0 0.0 15.966815784901307 2.093377182607385E-4 21 2.093377182607385E-4 0.0 0.0 16.386119234577567 2.093377182607385E-4 22 2.093377182607385E-4 0.0 0.0 16.788047653638184 2.093377182607385E-4 23 2.093377182607385E-4 0.0 0.0 17.230587590041385 2.093377182607385E-4 24 2.093377182607385E-4 0.0 0.0 17.53789536044815 2.093377182607385E-4 25 2.093377182607385E-4 0.0 0.0 17.82091995553667 2.093377182607385E-4 26 2.093377182607385E-4 0.0 0.0 18.060402305226955 2.093377182607385E-4 27 2.093377182607385E-4 0.0 0.0 18.29988465491724 2.093377182607385E-4 28 2.093377182607385E-4 0.0 0.0 18.543763096691 2.093377182607385E-4 29 2.093377182607385E-4 0.0 0.0 18.79120027967519 2.093377182607385E-4 30 2.093377182607385E-4 0.0 0.0 19.132211422721934 2.093377182607385E-4 31 2.093377182607385E-4 0.0 0.0 19.4053971450522 2.093377182607385E-4 32 2.093377182607385E-4 0.0 0.0 19.66895333234247 2.093377182607385E-4 33 2.093377182607385E-4 0.0 0.0 19.942139054672733 2.093377182607385E-4 34 2.093377182607385E-4 0.0 0.0 20.18287743067258 2.093377182607385E-4 35 2.093377182607385E-4 0.0 0.0 20.48871983705152 2.093377182607385E-4 36 2.093377182607385E-4 0.0 0.0 20.760440195353958 2.093377182607385E-4 37 2.093377182607385E-4 0.0 0.0 21.000341220480767 2.093377182607385E-4 38 2.093377182607385E-4 0.0 0.0 21.28252846469624 2.093377182607385E-4 39 2.093377182607385E-4 0.0 0.0 21.581672064090835 2.093377182607385E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGATCGG 20 7.029141E-4 45.000004 2 GTCGCTC 20 7.029141E-4 45.000004 13 CGAACAC 20 7.029141E-4 45.000004 14 CTACTGA 20 7.029141E-4 45.000004 29 ACGTTGT 20 7.029141E-4 45.000004 39 CAATTAG 20 7.029141E-4 45.000004 1 CGTTCCG 20 7.029141E-4 45.000004 1 TTATCCG 35 1.2099372E-7 45.000004 1 ATCCGTG 20 7.029141E-4 45.000004 26 TATTGGT 20 7.029141E-4 45.000004 27 TCCAGTA 20 7.029141E-4 45.000004 32 GTTCGGA 20 7.029141E-4 45.000004 15 GTATGCG 20 7.029141E-4 45.000004 1 CGTAGCG 40 6.8012014E-9 45.000004 1 TGCGAAA 20 7.029141E-4 45.000004 29 ACTACCG 35 1.2099372E-7 45.000004 1 GATATCG 20 7.029141E-4 45.000004 40 GTCGTAC 20 7.029141E-4 45.000004 22 TGTTACG 40 6.8012014E-9 45.000004 1 TTGCGAA 20 7.029141E-4 45.000004 15 >>END_MODULE