##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552980_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	491119
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16942126042772	31.0	31.0	34.0	28.0	34.0
2	31.382958101804245	31.0	31.0	34.0	28.0	34.0
3	31.371649233688782	31.0	31.0	34.0	28.0	34.0
4	35.40193720870094	37.0	35.0	37.0	33.0	37.0
5	35.070361765682044	37.0	35.0	37.0	32.0	37.0
6	35.13051012076503	37.0	35.0	37.0	32.0	37.0
7	35.08737597201493	37.0	35.0	37.0	32.0	37.0
8	35.45738812792826	37.0	35.0	37.0	33.0	37.0
9	35.8940114310381	39.0	37.0	39.0	32.0	39.0
10	35.715134213907426	38.0	35.0	39.0	30.0	39.0
11	36.08544772244609	38.0	35.0	39.0	31.0	39.0
12	35.73781303512998	37.0	35.0	39.0	31.0	39.0
13	35.38987495902215	37.0	35.0	39.0	30.0	39.0
14	36.42596397207194	38.0	35.0	40.0	31.0	41.0
15	36.50331793312822	38.0	35.0	40.0	31.0	41.0
16	36.582284537963304	38.0	35.0	40.0	31.0	41.0
17	36.56485088135462	38.0	35.0	40.0	31.0	41.0
18	36.44872016761722	38.0	35.0	40.0	31.0	41.0
19	36.47826086956522	38.0	35.0	40.0	31.0	41.0
20	36.39945308570835	38.0	35.0	40.0	31.0	41.0
21	36.38178730613151	38.0	35.0	40.0	31.0	41.0
22	36.41210175130671	38.0	35.0	40.0	31.0	41.0
23	36.31554266888473	38.0	35.0	40.0	31.0	41.0
24	35.93322392332612	38.0	34.0	40.0	30.0	41.0
25	36.093958897945306	38.0	34.0	40.0	30.0	41.0
26	35.982839189687226	38.0	34.0	40.0	30.0	41.0
27	35.838806887943655	38.0	34.0	40.0	30.0	41.0
28	35.814077647169015	38.0	34.0	40.0	30.0	41.0
29	35.54328991547873	37.0	34.0	40.0	29.0	41.0
30	35.56339502238765	38.0	34.0	40.0	29.0	41.0
31	35.49623818259933	38.0	34.0	40.0	29.0	41.0
32	35.434656366379635	38.0	34.0	40.0	29.0	41.0
33	35.362832633231456	37.0	34.0	40.0	28.0	41.0
34	35.254579847246795	37.0	34.0	40.0	27.0	41.0
35	35.17275242863746	37.0	34.0	40.0	27.0	41.0
36	35.02484326609233	37.0	34.0	40.0	27.0	41.0
37	34.863153329437466	37.0	33.0	40.0	26.0	41.0
38	34.804405856829	37.0	33.0	40.0	26.0	41.0
39	34.722069396622814	37.0	33.0	40.0	26.0	41.0
40	34.54836811444884	37.0	33.0	40.0	25.0	41.0
41	34.63254119673643	37.0	33.0	40.0	26.0	41.0
42	34.569650125529655	36.0	33.0	40.0	26.0	41.0
43	34.45896412071209	36.0	33.0	40.0	25.0	41.0
44	34.36412967122021	36.0	33.0	40.0	25.0	41.0
45	34.124886229203106	36.0	33.0	40.0	24.0	41.0
46	33.95234963420271	35.0	33.0	39.0	23.0	41.0
47	33.78716563602711	35.0	33.0	39.0	23.0	41.0
48	33.75252840961152	35.0	33.0	39.0	23.0	41.0
49	33.568872309969684	35.0	32.0	39.0	23.0	40.0
50	33.450416294217895	35.0	32.0	39.0	23.0	40.0
51	31.65040652061924	34.0	29.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	1.0
10	10.0
11	8.0
12	13.0
13	22.0
14	32.0
15	54.0
16	126.0
17	222.0
18	503.0
19	899.0
20	1408.0
21	1984.0
22	2668.0
23	3477.0
24	4277.0
25	4984.0
26	6009.0
27	7343.0
28	8983.0
29	11420.0
30	14052.0
31	17930.0
32	23407.0
33	32848.0
34	52618.0
35	54302.0
36	49205.0
37	64748.0
38	76858.0
39	50702.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.24132644023139	31.921998538032536	21.013644350961783	26.823030670774294
2	23.781405321317237	31.47933596541775	21.08653910762972	23.652719605635294
3	21.637729348691458	31.614944646816763	19.262541257821425	27.48478474667036
4	19.825337647291185	33.26566473705965	19.263152107737636	27.645845507911528
5	18.45112895245348	35.375133114377576	18.57390978561204	27.599828147556906
6	20.490553206045785	37.79980004846076	19.29756331968423	22.41208342580922
7	81.20557339463551	7.904805149057561	5.794929538462165	5.0946919178447585
8	83.7460981961602	6.208882165014996	4.974150867712306	5.0708687711125
9	75.6908203510758	11.676396148387662	8.16115849722776	4.4716250033087706
10	44.77631694151519	28.659652752184293	11.389500304406875	15.174530001893634
11	36.38222100957202	25.725129754703037	21.25309751811679	16.639551717608157
12	34.48838265267685	25.133419802532586	23.81520568334762	16.56299186144295
13	24.90190768428833	33.41613743308648	20.82794597643341	20.85400890619178
14	18.30493220584013	34.574308874223966	26.88411566239547	20.236643257540432
15	17.37318246697847	28.605287109641452	32.87268462429676	21.14884579908332
16	19.312630950950787	25.658750730474694	32.66662458589466	22.36199373267986
17	19.18801756804359	26.370594499500122	26.915676241399744	27.525711691056546
18	22.167336226047045	26.934001738886092	26.6448661118792	24.25379592318766
19	24.442752163935825	30.373697617074473	24.220810027712226	20.962740191277472
20	27.709984749113758	28.5957171276208	23.083611100364678	20.610687022900763
21	21.88715973114459	31.83179636707193	23.483106945567165	22.797936956216315
22	22.655201692461503	27.582317116625504	23.997035341739988	25.765445849173013
23	22.816873303618877	31.03646977616423	21.744831700667252	24.401825219549643
24	20.536570566400403	27.99830590956571	28.130249491467445	23.334874032566443
25	21.258595167362696	27.468902648848854	25.944424874623056	25.328077309165398
26	22.771059559902998	31.027307027421053	22.44588378783961	23.75574962483634
27	19.700317031106515	30.83488930381435	25.4062660984405	24.058527566638634
28	22.036614343977732	28.636033222090777	28.227374628145114	21.099977805786377
29	20.40564506769235	28.70383756279028	27.77290229048357	23.117615079033797
30	23.308403869530604	27.671908437669895	26.034016195667448	22.98567149713206
31	29.386360535837547	26.18917207438523	21.322123558648716	23.102343831128504
32	26.827103003549034	25.257625952162304	25.160704432123378	22.75456661216528
33	26.46649793634537	25.587281290277918	23.562110201397218	24.3841105719795
34	21.24434200265109	25.806983643475412	28.022129056297963	24.926545297575537
35	23.41163750537039	24.631504788045262	28.30169470128421	23.655163005300142
36	26.08451312207428	26.181231025474478	26.644255261962986	21.090000590488252
37	22.697757569957584	28.550310617182394	26.447561588942804	22.304370223917218
38	23.829662464697964	28.72318114347032	25.81309214263753	21.63406424919419
39	22.41371235891912	26.245981116592926	26.826492153632824	24.513814370855126
40	24.206760479639357	24.589763377104124	25.190839694656486	26.012636448600034
41	20.60193150743506	21.729153219484484	29.716626723869368	27.952288549211087
42	24.51137097119028	23.700162282461072	24.883989420079452	26.904477326269195
43	22.357106933350167	24.59078146029781	26.161480211516963	26.89063139483506
44	22.9410794532486	24.377594839539906	25.815535542302374	26.865790164909114
45	24.738607140021056	26.719593418295766	24.655938784693728	23.885860656989447
46	21.486849419387156	26.586428136561608	28.797501216609415	23.12922122744182
47	22.08935105341068	25.41807586348726	26.633259963471172	25.859313119630883
48	23.749030275758017	21.234772020630437	31.671346455746978	23.34485124786457
49	22.98343171410595	22.912573123825386	29.800720395667852	24.30327476640081
50	21.21522482331166	25.093307324701346	26.613509149513664	27.07795870247333
51	19.956059529360502	24.39734565349742	29.92003974596788	25.726555071174197
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3229.0
1	2607.0
2	1985.0
3	1387.5
4	790.0
5	699.0
6	608.0
7	643.0
8	678.0
9	808.5
10	939.0
11	925.0
12	911.0
13	951.5
14	992.0
15	1069.0
16	1146.0
17	1340.5
18	1535.0
19	1554.5
20	1574.0
21	1532.5
22	1491.0
23	1526.5
24	1562.0
25	1726.5
26	2286.5
27	2682.0
28	2995.5
29	3309.0
30	4375.5
31	5442.0
32	5964.5
33	6487.0
34	7333.5
35	8180.0
36	9690.0
37	11200.0
38	11896.5
39	12593.0
40	15549.5
41	18506.0
42	25586.0
43	32666.0
44	37127.0
45	41588.0
46	42980.0
47	44372.0
48	45787.5
49	47203.0
50	44617.0
51	42031.0
52	39025.0
53	36019.0
54	33330.5
55	30642.0
56	28439.0
57	26236.0
58	24485.5
59	22735.0
60	21641.5
61	20548.0
62	19276.5
63	18005.0
64	15835.5
65	13666.0
66	11263.0
67	8860.0
68	7724.0
69	6588.0
70	5742.0
71	4896.0
72	3900.0
73	2904.0
74	2466.0
75	1650.5
76	1273.0
77	911.5
78	550.0
79	401.0
80	252.0
81	196.0
82	140.0
83	116.0
84	92.0
85	67.0
86	42.0
87	31.5
88	21.0
89	17.5
90	14.0
91	9.0
92	4.0
93	5.0
94	6.0
95	5.0
96	4.0
97	3.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	491119.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.885258937065668
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.88515534171917	22.78618138336207
2	9.363627065471965	5.40941584752548
3	3.270402507753926	2.833992697947032
4	1.4771613058434783	1.706727472444117
5	0.8154234894303947	1.1776859317781292
6	0.5353261097410499	0.9277819977385354
7	0.3741998073496188	0.7566200830645678
8	0.2469937471616764	0.5707582674080921
9	0.23276364508892072	0.6051094343575856
>10	3.179668576169851	23.077366866664565
>50	1.3595694226911803	27.698715915382383
>100	0.2519035203838043	9.448734663700902
>500	0.005676699050902632	1.2425048164028474
>1k	0.002128762144088487	1.7584046222236929
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGCT	4504	0.9170893408725787	TruSeq Adapter, Index 13 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2769	0.5638144726634482	No Hit
AAACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCT	1306	0.26592333019084985	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCC	980	0.19954430596250605	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGT	942	0.1918068736904905	No Hit
AATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCT	868	0.1767392424239339	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTT	849	0.17287052628792615	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	735	0.1496582294718795	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	636	0.12950018223689166	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	543	0.11056383483432732	No Hit
CCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	509	0.10364086911726078	TruSeq Adapter, Index 16 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1909537199741813	0.0
2	0.0	0.0	0.0	1.474388081096435	0.0
3	0.0	0.0	0.0	1.8050615024057306	0.0
4	0.0	0.0	0.0	3.555553745629878	0.0
5	0.0	0.0	0.0	3.767925899832831	0.0
6	0.0	0.0	0.0	4.981073833429373	0.0
7	0.0	0.0	0.0	5.9592481659231265	0.0
8	0.0	0.0	0.0	6.4405978998979885	0.0
9	0.0	0.0	0.0	7.556417080178123	0.0
10	0.0	0.0	0.0	8.857120168431683	0.0
11	0.0	0.0	0.0	10.474243513282932	0.0
12	0.0	0.0	0.0	11.15880265271757	0.0
13	0.0	0.0	0.0	11.437961064426341	0.0
14	0.0	0.0	0.0	11.756824720688876	0.0
15	0.0	0.0	0.0	11.992205555069138	0.0
16	0.0	0.0	0.0	12.524866682005786	0.0
17	0.0	0.0	0.0	13.141214247463445	0.0
18	0.0	0.0	0.0	13.92900702273787	0.0
19	0.0	0.0	0.0	14.265585326570546	2.0361663873725105E-4
20	0.0	0.0	0.0	14.593000881660046	2.0361663873725105E-4
21	0.0	0.0	0.0	14.990256943836423	2.0361663873725105E-4
22	0.0	0.0	0.0	15.384255139793003	2.0361663873725105E-4
23	0.0	0.0	0.0	15.810017531392596	2.0361663873725105E-4
24	0.0	0.0	0.0	16.111370156723726	2.0361663873725105E-4
25	2.0361663873725105E-4	0.0	0.0	16.388085168767653	2.0361663873725105E-4
26	2.0361663873725105E-4	0.0	0.0	16.615117720959685	2.0361663873725105E-4
27	2.0361663873725105E-4	0.0	0.0	16.846222605926467	2.0361663873725105E-4
28	2.0361663873725105E-4	0.0	0.0	17.09096980568864	2.0361663873725105E-4
29	2.0361663873725105E-4	0.0	0.0	17.321260224100474	2.0361663873725105E-4
30	2.0361663873725105E-4	0.0	0.0	17.662521710624105	2.0361663873725105E-4
31	2.0361663873725105E-4	0.0	0.0	17.92355824148526	2.0361663873725105E-4
32	2.0361663873725105E-4	0.0	0.0	18.19538645419949	2.0361663873725105E-4
33	2.0361663873725105E-4	0.0	0.0	18.46069893447413	2.0361663873725105E-4
34	2.0361663873725105E-4	0.0	0.0	18.709111233733577	2.0361663873725105E-4
35	2.0361663873725105E-4	0.0	0.0	19.02654957352495	2.0361663873725105E-4
36	2.0361663873725105E-4	0.0	0.0	19.274147406229446	2.0361663873725105E-4
37	2.0361663873725105E-4	0.0	0.0	19.508713774054762	2.0361663873725105E-4
38	2.0361663873725105E-4	0.0	0.0	19.78196730324015	2.0361663873725105E-4
39	2.0361663873725105E-4	0.0	0.0	20.088206727900978	2.0361663873725105E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGG	35	1.2100281E-7	45.0	2
GCATAAC	25	3.8875318E-5	45.0	23
TCACGCG	25	3.8875318E-5	45.0	1
TTGATCG	25	3.8875318E-5	45.0	1
CTTCGAC	20	7.0293376E-4	45.0	37
GTACCGC	20	7.0293376E-4	45.0	10
GGTACCG	20	7.0293376E-4	45.0	9
CCCCTAG	25	3.8875318E-5	45.0	1
CGAACAC	20	7.0293376E-4	45.0	14
TCCGCAC	20	7.0293376E-4	45.0	25
TTGGACG	20	7.0293376E-4	45.0	38
ATCTACG	20	7.0293376E-4	45.0	1
ACGTTAT	40	6.8012014E-9	45.0	16
GCGAACC	25	3.8875318E-5	45.0	36
GGGTCGT	25	3.8875318E-5	45.0	7
TCTTCGA	20	7.0293376E-4	45.0	36
CCGGCAC	25	3.8875318E-5	45.0	16
TTATGTC	20	7.0293376E-4	45.0	29
CTATACG	20	7.0293376E-4	45.0	1
TGGGTAC	25	3.8875318E-5	45.0	6
>>END_MODULE