FastQCFastQC Report
Sat 18 Jun 2016
SRR3552976_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552976_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603557
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGCT42820.7094607468722921TruSeq Adapter, Index 13 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCC37340.6186656769783135No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGT33580.5563683297517882No Hit
AATCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCT32610.5402969396428174No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14740.24421885588270867No Hit
AAACTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCT8580.14215724446903938No Hit
AATGACTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTT7320.12128100577078883No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG7010.11614478831328276No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGATCG302.1637825E-645.00000427
TCCGATT302.1637825E-645.00000437
ACGTTAG302.1637825E-645.0000041
GCAAACG600.045.0000041
TTATCCG302.1637825E-645.0000041
CGTTAGC302.1637825E-645.00000417
TATAGCG302.1637825E-645.0000041
ACGTAGG302.1637825E-645.0000042
ACTCACG302.1637825E-645.0000041
TCGACAG302.1637825E-645.0000041
CTATGCG253.8885966E-545.01
TTAGGCG207.030621E-445.01
CGAACAT207.030621E-445.03
GTCGATG207.030621E-445.013
ATCTACG253.8885966E-545.01
CTCCGAT207.030621E-445.036
GGTCGAT207.030621E-445.012
CCGATTC207.030621E-445.038
CGTGCGA207.030621E-445.037
CGCATTG453.8380676E-1045.01