##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552976_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	603557
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.223218353858872	31.0	31.0	34.0	28.0	34.0
2	31.43044484613715	31.0	31.0	34.0	30.0	34.0
3	31.396231010492794	31.0	31.0	34.0	28.0	34.0
4	35.42594982743966	37.0	35.0	37.0	33.0	37.0
5	35.103239627740216	37.0	35.0	37.0	32.0	37.0
6	35.17177830759978	37.0	35.0	37.0	32.0	37.0
7	35.20394759732718	37.0	35.0	37.0	32.0	37.0
8	35.539857875892416	37.0	35.0	37.0	33.0	37.0
9	35.97047172015236	39.0	37.0	39.0	32.0	39.0
10	35.68790851568286	38.0	35.0	39.0	30.0	39.0
11	36.13821726862583	38.0	35.0	39.0	31.0	39.0
12	36.01958058642349	37.0	35.0	39.0	32.0	39.0
13	35.83159502747876	37.0	35.0	39.0	31.0	39.0
14	36.88117609438711	39.0	35.0	40.0	31.0	41.0
15	36.8941707245546	38.0	35.0	40.0	32.0	41.0
16	36.893009276671464	38.0	35.0	40.0	32.0	41.0
17	36.875411932924315	38.0	35.0	40.0	32.0	41.0
18	36.757177863896864	38.0	35.0	40.0	31.0	41.0
19	36.8321765798425	39.0	35.0	40.0	31.0	41.0
20	36.79829245622203	38.0	35.0	40.0	31.0	41.0
21	36.83085276121394	38.0	35.0	40.0	31.0	41.0
22	36.83983782807589	39.0	35.0	40.0	31.0	41.0
23	36.802088286607564	38.0	35.0	40.0	31.0	41.0
24	36.42545608782601	38.0	35.0	40.0	30.0	41.0
25	36.56922378499463	38.0	35.0	40.0	31.0	41.0
26	36.45389747778586	38.0	35.0	40.0	31.0	41.0
27	36.398819001353644	38.0	35.0	40.0	30.0	41.0
28	36.35807554216089	38.0	35.0	40.0	30.0	41.0
29	36.10900875973603	38.0	35.0	40.0	30.0	41.0
30	36.168012300412386	38.0	35.0	40.0	30.0	41.0
31	36.132431568186604	38.0	35.0	40.0	30.0	41.0
32	36.04776516551046	38.0	35.0	40.0	30.0	41.0
33	36.06025942868693	38.0	35.0	40.0	30.0	41.0
34	36.038819862912696	38.0	35.0	40.0	30.0	41.0
35	35.948308113401055	38.0	35.0	40.0	30.0	41.0
36	35.83758949030498	38.0	35.0	40.0	30.0	41.0
37	35.72090291389214	38.0	34.0	40.0	29.0	41.0
38	35.69242010282376	38.0	34.0	40.0	29.0	41.0
39	35.54484332051488	38.0	34.0	40.0	29.0	41.0
40	35.397887854833925	38.0	34.0	40.0	28.0	41.0
41	35.47390055951633	38.0	34.0	40.0	29.0	41.0
42	35.429530268060844	38.0	34.0	40.0	28.0	41.0
43	35.362348543716664	38.0	34.0	40.0	28.0	41.0
44	35.31455355500806	37.0	34.0	40.0	28.0	41.0
45	35.08352152323641	37.0	34.0	40.0	27.0	41.0
46	34.91299910364721	37.0	34.0	40.0	27.0	41.0
47	34.756236776311106	37.0	33.0	40.0	26.0	41.0
48	34.68044277508172	37.0	33.0	40.0	26.0	41.0
49	34.52352636122189	37.0	33.0	40.0	26.0	41.0
50	34.411162491695066	36.0	33.0	40.0	25.0	41.0
51	32.5307253498841	35.0	30.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	6.0
10	12.0
11	11.0
12	15.0
13	13.0
14	33.0
15	63.0
16	110.0
17	199.0
18	301.0
19	586.0
20	985.0
21	1479.0
22	2052.0
23	2721.0
24	3513.0
25	4301.0
26	5498.0
27	7024.0
28	8987.0
29	11793.0
30	15279.0
31	20178.0
32	26506.0
33	37051.0
34	57139.0
35	63045.0
36	66387.0
37	87445.0
38	108280.0
39	72541.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.35681799730597	32.77436928078044	19.902014225665514	26.966798496248074
2	23.548065882758383	32.435544613019154	21.216554525918845	22.799834978303622
3	21.066444428612378	29.790889675705856	22.6111204078488	26.531545487832965
4	21.321929826014777	30.974373588575727	19.993140664427717	27.71055592098178
5	17.366379645998638	35.28515119533035	18.982796985206036	28.365672173464972
6	21.333030683100354	35.84748416471021	20.52780433331069	22.29168081887875
7	82.5443164440144	6.509907100737792	6.003244101219934	4.942532354027872
8	84.83888017204671	4.262894805295938	4.5897901938010826	6.308434828856265
9	76.52616074372429	10.23449317960027	8.289357923112481	4.949988153562961
10	39.4887309732138	33.63543128486622	12.353597091906812	14.52224065001317
11	30.54326269101344	24.817871385801173	26.11600892707731	18.52285699610807
12	30.22829658176444	23.10651686584697	29.17106420768875	17.49412234469984
13	24.928051534486386	30.36382644886896	25.06291866385445	19.645203352790208
14	19.48647766490986	30.900146962093057	29.810440438931202	19.80293493406588
15	17.51880932538269	28.511971528786844	31.551452472591652	22.417766673238816
16	19.920239513417954	25.905755380187788	32.21071083592768	21.963294270466584
17	20.083272996585244	25.841801188620128	28.253835180438635	25.821090634355993
18	22.240650013171912	25.630056481823587	28.561511174586656	23.567782330417838
19	23.44053668501898	28.508326471236355	27.416797419299254	20.634339424445415
20	26.013450262361303	28.20678080115051	25.426264627864477	20.35350430862371
21	22.085900751710277	28.57112087176522	26.007319938299116	23.335658438225387
22	22.628020220128338	26.32825068717619	26.167536786086483	24.876192306608985
23	22.325314759003707	28.090636012837226	23.662222457862306	25.921826770296757
24	19.318307964285065	29.16708778127004	28.50501278255409	23.009591471890808
25	20.897446305817013	26.51481135998754	27.622908855335947	24.964833478859493
26	23.43424067652268	30.1898577930502	23.609866176682566	22.766035353744552
27	19.28367991755543	28.221029662484238	28.17778602518072	24.317504394779615
28	20.380013818081803	26.649844173789717	30.204935076554495	22.765206931573985
29	22.199725957945976	28.07771262697641	27.497982792014675	22.22457862306294
30	21.478501616251656	26.381435390526498	29.026256012273905	23.113806980947945
31	25.377884773103453	27.013190137799743	23.6570862404048	23.951838848692002
32	25.06938035678486	26.539166971802164	25.153050996011977	23.238401675400997
33	23.401435158568287	24.765018051319096	26.17482690118746	25.65871988892516
34	21.003318659215285	25.858535316465552	28.51942732832193	24.61871869599723
35	23.391659776955613	24.783574707939763	28.55256421514455	23.27220129996007
36	24.79682946266881	25.932430574079994	28.003651684927856	21.26708827832334
37	21.68560715889303	29.074304498166704	25.645465134196105	23.59462320874416
38	22.259040985358467	27.929922111747523	25.36131632969214	24.44972057320187
39	21.478501616251656	26.98137872645003	26.989497263721567	24.550622393576745
40	24.250236514529696	25.338783246652763	25.280959379147287	25.130020859670253
41	21.659760387171385	21.93529360110147	30.487261352283213	25.91768465944393
42	25.60437539453606	23.790462209865844	25.69931257528286	24.905849820315233
43	22.86594306751475	23.62030429603169	27.39393296739165	26.11981966906191
44	22.335255825050492	24.179820630031628	26.268272922027247	27.216650622890633
45	24.391896705696396	26.42617018773703	24.883813790578188	24.29811931598838
46	20.69083781647798	25.524681181727658	30.346429583287083	23.438051418507282
47	22.127984597975008	25.65026998278539	26.00118961423693	26.220555805002675
48	22.75957366081414	22.24363233298595	31.97146251306836	23.025331493131553
49	22.291846503312858	23.014230636045973	29.551309983978314	25.142612876662852
50	21.18159511032098	24.185453900791472	26.81105512818176	27.82189586070578
51	21.034301648394436	23.666861622017475	30.051511290565763	25.247325439022326
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1784.0
1	1433.0
2	1082.0
3	748.5
4	415.0
5	372.5
6	330.0
7	374.5
8	419.0
9	470.0
10	521.0
11	575.5
12	630.0
13	663.0
14	696.0
15	744.0
16	792.0
17	818.5
18	845.0
19	1075.0
20	1305.0
21	1227.0
22	1149.0
23	1699.5
24	2250.0
25	2303.0
26	3162.5
27	3969.0
28	4509.0
29	5049.0
30	6382.0
31	7715.0
32	10073.0
33	12431.0
34	12811.0
35	13191.0
36	15194.0
37	17197.0
38	18260.0
39	19323.0
40	22157.5
41	24992.0
42	30958.0
43	36924.0
44	46589.5
45	56255.0
46	58965.0
47	61675.0
48	62189.5
49	62704.0
50	58537.5
51	54371.0
52	50815.0
53	47259.0
54	41328.0
55	35397.0
56	32196.5
57	28996.0
58	26860.5
59	24725.0
60	22679.0
61	20633.0
62	19263.5
63	17894.0
64	15454.0
65	13014.0
66	10618.5
67	8223.0
68	6987.0
69	5751.0
70	4833.0
71	3915.0
72	3341.5
73	2768.0
74	2260.0
75	1436.0
76	1120.0
77	1014.5
78	909.0
79	691.5
80	474.0
81	335.0
82	196.0
83	129.0
84	62.0
85	50.0
86	38.0
87	29.5
88	21.0
89	21.0
90	21.0
91	16.0
92	11.0
93	8.0
94	5.0
95	3.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	603557.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.356376123222912
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.18770288755667	23.431389409422497
2	9.849792798370386	5.980080298462875
3	3.4185089010294862	3.1132062594070953
4	1.6707460083584875	2.028711769444143
5	0.9590438937660033	1.4556548578920518
6	0.5669959202604156	1.032716484945485
7	0.4728519744565325	1.0047850671047764
8	0.3646949289479539	0.8856653146700916
9	0.31076678257103807	0.8490378004497241
>10	3.9924076055492903	29.430004862140986
>50	1.0883552653110755	22.400117773262735
>100	0.11211699969284929	5.0595424700210545
>500	0.0032814731617419298	0.654627595054734
>1k	0.002734560968118275	2.6744600377217123
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGCT	4282	0.7094607468722921	TruSeq Adapter, Index 13 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCC	3734	0.6186656769783135	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGT	3358	0.5563683297517882	No Hit
AATCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCT	3261	0.5402969396428174	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1474	0.24421885588270867	No Hit
AAACTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCT	858	0.14215724446903938	No Hit
AATGACTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTT	732	0.12128100577078883	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	701	0.11614478831328276	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.31368868226199E-4	0.0	0.0	0.9488747541657209	0.0
2	3.31368868226199E-4	0.0	0.0	1.2018748850564238	0.0
3	3.31368868226199E-4	0.0	0.0	1.602002793439559	0.0
4	3.31368868226199E-4	0.0	0.0	3.6770346462720176	0.0
5	3.31368868226199E-4	0.0	0.0	3.95621291775259	0.0
6	3.31368868226199E-4	0.0	0.0	5.189733529724616	0.0
7	3.31368868226199E-4	0.0	0.0	6.139105337192676	0.0
8	3.31368868226199E-4	0.0	0.0	6.6058383880892775	0.0
9	3.31368868226199E-4	0.0	0.0	8.165094597527657	0.0
10	3.31368868226199E-4	0.0	0.0	9.554855630868335	0.0
11	3.31368868226199E-4	0.0	0.0	11.714552229532588	0.0
12	3.31368868226199E-4	0.0	0.0	12.459966498607422	0.0
13	3.31368868226199E-4	0.0	0.0	12.791501051267735	0.0
14	3.31368868226199E-4	0.0	0.0	13.189640746441512	0.0
15	3.31368868226199E-4	0.0	0.0	13.462688693859901	0.0
16	3.31368868226199E-4	0.0	0.0	14.048383168449707	0.0
17	3.31368868226199E-4	0.0	0.0	14.816330520563923	0.0
18	3.31368868226199E-4	0.0	0.0	15.703239296371345	0.0
19	3.31368868226199E-4	0.0	0.0	16.13004239864669	0.0
20	3.31368868226199E-4	0.0	0.0	16.557508238658485	0.0
21	3.31368868226199E-4	0.0	0.0	17.07096429997498	0.0
22	3.31368868226199E-4	0.0	0.0	17.658481303340032	0.0
23	3.31368868226199E-4	0.0	0.0	18.23937092934056	0.0
24	3.31368868226199E-4	0.0	0.0	18.64943990377048	0.0
25	3.31368868226199E-4	0.0	0.0	18.998205637578554	0.0
26	3.31368868226199E-4	0.0	0.0	19.32924313693653	0.0
27	3.31368868226199E-4	0.0	0.0	19.641889664107946	0.0
28	3.31368868226199E-4	0.0	0.0	19.97789769648931	0.0
29	3.31368868226199E-4	0.0	0.0	20.33809565625119	0.0
30	3.31368868226199E-4	0.0	0.0	20.795384694403346	0.0
31	3.31368868226199E-4	0.0	0.0	21.15425717869232	0.0
32	4.970533023392986E-4	0.0	0.0	21.51147281864016	0.0
33	4.970533023392986E-4	0.0	0.0	21.86156402792114	0.0
34	4.970533023392986E-4	0.0	0.0	22.21579734805495	0.0
35	4.970533023392986E-4	0.0	0.0	22.611783145585257	0.0
36	4.970533023392986E-4	0.0	0.0	22.982087855828034	0.0
37	4.970533023392986E-4	0.0	0.0	23.330522220767882	0.0
38	4.970533023392986E-4	0.0	0.0	23.690057442793307	0.0
39	4.970533023392986E-4	0.0	0.0	24.057048464353823	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGATCG	30	2.1637825E-6	45.000004	27
TCCGATT	30	2.1637825E-6	45.000004	37
ACGTTAG	30	2.1637825E-6	45.000004	1
GCAAACG	60	0.0	45.000004	1
TTATCCG	30	2.1637825E-6	45.000004	1
CGTTAGC	30	2.1637825E-6	45.000004	17
TATAGCG	30	2.1637825E-6	45.000004	1
ACGTAGG	30	2.1637825E-6	45.000004	2
ACTCACG	30	2.1637825E-6	45.000004	1
TCGACAG	30	2.1637825E-6	45.000004	1
CTATGCG	25	3.8885966E-5	45.0	1
TTAGGCG	20	7.030621E-4	45.0	1
CGAACAT	20	7.030621E-4	45.0	3
GTCGATG	20	7.030621E-4	45.0	13
ATCTACG	25	3.8885966E-5	45.0	1
CTCCGAT	20	7.030621E-4	45.0	36
GGTCGAT	20	7.030621E-4	45.0	12
CCGATTC	20	7.030621E-4	45.0	38
CGTGCGA	20	7.030621E-4	45.0	37
CGCATTG	45	3.8380676E-10	45.0	1
>>END_MODULE