FastQCFastQC Report
Sat 18 Jun 2016
SRR3552971_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552971_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510932
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGCT29920.5855965177362153No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCC22910.44839626408210875No Hit
AATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCT19800.38752710732543666No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGT18540.36286629140472704No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15900.31119601042800216No Hit
AAACTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCT7340.14365903877619723No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG6320.12369552112609897No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA5820.11390948306232532No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG600.045.0000041
CGGGTAC302.1631258E-645.0000046
TCGTTCG406.8030204E-945.00000445
CGACGTC207.029602E-445.0000041
CGACGAT207.029602E-445.00000434
GCGAAGT207.029602E-445.00000417
GCGCGAC302.1631258E-645.0000049
GACGTGC207.029602E-445.00000439
GACGTAA207.029602E-445.00000442
CGCATAG406.8030204E-945.0000041
TGAACGT207.029602E-445.00000421
TGTATCG351.2101555E-745.00000435
CGTCTAT207.029602E-445.00000439
CTAAGCG302.1631258E-645.0000041
TGACGTA207.029602E-445.00000441
TACGGGT600.045.0000044
CACGAAC207.029602E-445.00000441
CGCGACG650.045.0000041
ACGGGTA650.045.0000045
ACCCTCG207.029602E-445.00000414