##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552971_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	510932
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.541737452341994	31.0	31.0	34.0	30.0	34.0
2	31.72658201091339	31.0	31.0	34.0	30.0	34.0
3	31.924939130843242	33.0	31.0	34.0	30.0	34.0
4	35.72596353330776	37.0	35.0	37.0	35.0	37.0
5	35.3437267581596	37.0	35.0	37.0	33.0	37.0
6	35.35969170065684	37.0	35.0	37.0	33.0	37.0
7	35.348095245551264	37.0	35.0	37.0	33.0	37.0
8	35.64919989352791	37.0	35.0	37.0	35.0	37.0
9	36.48856403591867	39.0	37.0	39.0	33.0	39.0
10	36.227255290332174	38.0	35.0	39.0	32.0	39.0
11	36.45190945174701	38.0	35.0	39.0	32.0	39.0
12	36.06353095911002	37.0	35.0	39.0	32.0	39.0
13	35.82488863488683	37.0	35.0	39.0	31.0	39.0
14	36.88130318711688	39.0	35.0	40.0	31.0	41.0
15	36.9049071109267	38.0	35.0	40.0	32.0	41.0
16	36.95109721058771	38.0	35.0	40.0	32.0	41.0
17	36.936079556575045	38.0	35.0	40.0	32.0	41.0
18	36.811659868632226	38.0	35.0	40.0	31.0	41.0
19	36.89444583623652	38.0	35.0	40.0	32.0	41.0
20	36.84210423304862	38.0	35.0	40.0	31.0	41.0
21	36.81221571559425	38.0	35.0	40.0	31.0	41.0
22	36.81689735620396	38.0	35.0	40.0	31.0	41.0
23	36.742539124580176	38.0	35.0	40.0	31.0	41.0
24	36.438469307070214	38.0	35.0	40.0	31.0	41.0
25	36.498806103356216	38.0	35.0	40.0	31.0	41.0
26	36.404271018452555	38.0	35.0	40.0	31.0	41.0
27	36.30899219465604	38.0	35.0	40.0	30.0	41.0
28	36.2646849287185	38.0	35.0	40.0	30.0	41.0
29	36.06485207424863	38.0	35.0	40.0	30.0	41.0
30	36.059409079877554	38.0	35.0	40.0	30.0	41.0
31	35.93518902711124	38.0	35.0	40.0	30.0	41.0
32	35.94521384450377	38.0	35.0	40.0	30.0	41.0
33	35.88692820179593	38.0	35.0	40.0	30.0	41.0
34	35.787363093327485	38.0	34.0	40.0	30.0	41.0
35	35.76146140778029	38.0	34.0	40.0	30.0	41.0
36	35.499035096646914	38.0	34.0	40.0	29.0	41.0
37	35.455831695802964	38.0	34.0	40.0	29.0	41.0
38	35.312241159293215	37.0	34.0	40.0	28.0	41.0
39	35.28054222479704	37.0	34.0	40.0	28.0	41.0
40	35.10949597989556	37.0	34.0	40.0	27.0	41.0
41	35.141120540502456	37.0	34.0	40.0	27.0	41.0
42	35.12661763209194	37.0	34.0	40.0	28.0	41.0
43	35.03339191908121	37.0	34.0	40.0	27.0	41.0
44	34.89993776079791	37.0	34.0	40.0	27.0	41.0
45	34.66298254953693	36.0	33.0	40.0	26.0	41.0
46	34.57487884884877	36.0	33.0	40.0	26.0	41.0
47	34.461247289267455	36.0	33.0	40.0	26.0	41.0
48	34.454258100882306	36.0	33.0	40.0	26.0	41.0
49	34.287145843282474	36.0	33.0	39.0	26.0	41.0
50	34.04361832885785	35.0	33.0	39.0	24.0	41.0
51	32.19383401313678	35.0	30.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	11.0
11	5.0
12	15.0
13	27.0
14	38.0
15	62.0
16	98.0
17	190.0
18	364.0
19	598.0
20	979.0
21	1448.0
22	1946.0
23	2647.0
24	3269.0
25	4025.0
26	4746.0
27	6046.0
28	7792.0
29	9876.0
30	12688.0
31	16671.0
32	21792.0
33	31020.0
34	51963.0
35	56826.0
36	51478.0
37	69398.0
38	88422.0
39	66491.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.143776471232965	33.16840597183187	20.30955195603329	26.37826560090188
2	25.04012275606147	30.59369935725302	19.90656290856709	24.45961497811842
3	21.07227576272381	31.194953535891273	20.642864412485416	27.089906288899503
4	21.15780573540119	31.574847533526967	19.87074600925368	27.396600721818164
5	18.81307101532102	35.27220843478192	18.26231279309184	27.652407756805207
6	20.76871286198555	36.56787987442556	20.658717794148732	22.004689469440162
7	82.86347302576468	7.2305120837998	5.127101062372292	4.778913828063225
8	85.23697869775233	5.082868170324035	4.254382187844958	5.425770944078664
9	78.3579811012033	9.684458988671683	7.457939608401901	4.499620301723126
10	44.01388051638966	29.713151652274668	11.301308197568364	14.971659633767311
11	35.76659907776377	26.135963298442842	20.50801280796662	17.589424815826764
12	33.5735479476721	25.154619401407626	24.809563699278964	16.462268951641317
13	25.569743136072905	33.05743230018868	21.570385100169887	19.80243946356854
14	19.888948040052295	33.087377576663826	27.164475898945454	19.859198484338425
15	18.14644610241676	28.583451418192634	31.30671009057957	21.963392388811034
16	20.130663180227508	26.193505202257832	31.795033389961873	21.880798227552788
17	20.18448638957826	26.543649644179656	26.00815764132996	27.263706324912125
18	25.062043481324324	25.66975644508467	25.99563151260833	23.272568560982677
19	25.79462629077842	28.43548652266838	23.63171615792317	22.138171028630033
20	29.839000101774797	28.385577728543133	21.770215997432143	20.005206172249927
21	23.9043551783799	29.979723329131865	23.565171099089508	22.55075039339873
22	24.391308432433277	27.305199126302522	23.203870573774985	25.099621867489212
23	24.329656392631506	30.236704688686554	21.347654873838398	24.085984044843542
24	20.32403529236767	28.351326595319925	28.308463748600598	23.016174363711805
25	21.431227638903025	27.01983825636288	26.50372260887946	25.045211495854637
26	24.91662295569665	29.80122599484863	22.545074491321742	22.73707655813298
27	21.15956722225267	29.082539359445093	25.02642230277219	24.731471115530052
28	20.65715202805853	29.84956902288367	28.852958906468963	20.64032004258884
29	22.313145389210305	29.005033937980006	26.89242404077255	21.78939663203714
30	22.24327307743496	27.69742353189857	26.453030931709108	23.606272458957356
31	27.729130295225197	29.376903384403402	20.39938778545873	22.49457853491267
32	27.091080613467156	26.200355428902476	23.43873548730555	23.269828470324818
33	23.401939984185763	28.216474990801128	23.97148739949739	24.410097625515725
34	21.174833441632153	27.376245762645517	26.157688302944422	25.291232492777905
35	22.863903611439486	26.368283842076835	27.7322618274056	23.035550719078078
36	25.928695012252117	29.51077638511583	23.683777880422443	20.876750722209607
37	23.093679785176892	31.28107067085248	20.499205373709223	25.1260441702614
38	22.930448670273147	32.029898303492445	22.306686604088217	22.732966422146195
39	22.647436449468813	27.834036623268847	23.964245731330198	25.554281195932138
40	24.993541214877908	27.353346433576288	22.688929250859214	24.964183100686586
41	21.529088019540758	25.559174214964024	26.12500293581142	26.786734829683795
42	26.62878817533449	24.225924389155505	23.185864263737642	25.959423171772368
43	23.192127328098454	24.753000399270352	25.65546882951156	26.399403443119635
44	23.994582449327893	25.40122756061472	24.051341470097782	26.552848519959603
45	24.62167176845451	28.055788245793963	23.282941761330274	24.039598224421255
46	21.02021404022453	27.83971252534584	26.749743605802728	24.390329828626903
47	22.359922651155145	28.259729279043004	24.224162902304023	25.15618516749783
48	23.019305895892213	23.81667227732849	29.034392052171327	24.12962977460797
49	23.944869375963926	24.70739746189317	27.212036043935395	24.13569711820751
50	21.34119608871631	27.325162643952623	24.89313646434359	26.44050480298748
51	21.246271519497704	26.169431548620953	27.372135626658732	25.212161305222615
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1877.0
1	1507.0
2	1137.0
3	805.0
4	473.0
5	413.5
6	354.0
7	402.5
8	451.0
9	507.0
10	563.0
11	598.0
12	633.0
13	733.5
14	834.0
15	907.0
16	980.0
17	1066.5
18	1153.0
19	1172.0
20	1191.0
21	1064.5
22	938.0
23	1287.0
24	1636.0
25	1471.0
26	2194.0
27	3082.0
28	3906.0
29	4730.0
30	5099.5
31	5469.0
32	7218.0
33	8967.0
34	9184.5
35	9402.0
36	10351.0
37	11300.0
38	13422.5
39	15545.0
40	18718.0
41	21891.0
42	26620.5
43	31350.0
44	35854.0
45	40358.0
46	43565.0
47	46772.0
48	47283.0
49	47794.0
50	45858.0
51	43922.0
52	41022.5
53	38123.0
54	35236.0
55	32349.0
56	30562.0
57	28775.0
58	26539.5
59	24304.0
60	22324.5
61	20345.0
62	18661.5
63	16978.0
64	15409.0
65	13840.0
66	11531.5
67	9223.0
68	8473.5
69	7724.0
70	6516.5
71	5309.0
72	4698.5
73	4088.0
74	3267.0
75	1938.5
76	1431.0
77	1156.5
78	882.0
79	730.5
80	579.0
81	377.0
82	175.0
83	135.0
84	95.0
85	82.5
86	70.0
87	58.0
88	46.0
89	33.0
90	20.0
91	17.5
92	15.0
93	9.5
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	510932.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.03837817457592
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.82011389817134	20.52347933443733
2	9.081521847701639	4.7293620054225745
3	3.041647505753465	2.3759870408569292
4	1.3852239476390453	1.4427594002041766
5	0.7871845315170971	1.0248504262409277
6	0.48950817747974074	0.7647599426858951
7	0.3637369417552367	0.6629784031840583
8	0.2569781851588687	0.5353036134226253
9	0.217894528520296	0.5106258122204148
>10	3.61020951640362	24.22817946844322
>50	1.6431892941391912	30.35222463175299
>100	0.2967808738843075	10.373693712969818
>500	0.0022540319535517025	0.3811024111808619
>1k	0.0037567199225861715	2.094693796978177
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGCT	2992	0.5855965177362153	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCC	2291	0.44839626408210875	No Hit
AATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCT	1980	0.38752710732543666	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGT	1854	0.36286629140472704	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1590	0.31119601042800216	No Hit
AAACTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCT	734	0.14365903877619723	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	632	0.12369552112609897	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	582	0.11390948306232532	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.914415225509461E-4	0.0	0.0	0.7946262907784206	0.0
2	3.914415225509461E-4	0.0	0.0	0.9856497537832823	0.0
3	3.914415225509461E-4	0.0	0.0	1.2735549936195032	0.0
4	3.914415225509461E-4	0.0	0.0	2.7929352634010005	0.0
5	3.914415225509461E-4	0.0	0.0	2.994527647514738	0.0
6	3.914415225509461E-4	0.0	0.0	3.9829174919558765	0.0
7	3.914415225509461E-4	0.0	0.0	4.720197599680584	0.0
8	3.914415225509461E-4	0.0	0.0	5.14275872327433	0.0
9	3.914415225509461E-4	0.0	0.0	6.195344977413824	0.0
10	3.914415225509461E-4	0.0	0.0	7.217007351271794	0.0
11	3.914415225509461E-4	0.0	0.0	8.790602271926597	0.0
12	3.914415225509461E-4	0.0	0.0	9.319831210415476	0.0
13	3.914415225509461E-4	0.0	0.0	9.57563824540252	0.0
14	3.914415225509461E-4	0.0	0.0	9.874308127108891	0.0
15	3.914415225509461E-4	0.0	0.0	10.094689704305074	0.0
16	3.914415225509461E-4	0.0	0.0	10.515880782569893	0.0
17	3.914415225509461E-4	0.0	0.0	11.034932241472447	0.0
18	3.914415225509461E-4	0.0	0.0	11.64479813360682	0.0
19	3.914415225509461E-4	0.0	0.0	11.942489411506815	0.0
20	3.914415225509461E-4	0.0	0.0	12.206320997706152	0.0
21	3.914415225509461E-4	0.0	0.0	12.539633454158283	0.0
22	3.914415225509461E-4	0.0	0.0	12.883906273241841	0.0
23	3.914415225509461E-4	0.0	0.0	13.235812202015142	0.0
24	3.914415225509461E-4	0.0	0.0	13.49357644461494	0.0
25	3.914415225509461E-4	0.0	0.0	13.714740904856223	0.0
26	3.914415225509461E-4	0.0	0.0	13.92885941769159	0.0
27	3.914415225509461E-4	0.0	0.0	14.144935138139713	0.0
28	3.914415225509461E-4	0.0	0.0	14.352594865852991	0.0
29	3.914415225509461E-4	0.0	0.0	14.580609552738917	0.0
30	3.914415225509461E-4	0.0	0.0	14.917053541371455	0.0
31	3.914415225509461E-4	0.0	0.0	15.166793232758959	0.0
32	3.914415225509461E-4	0.0	0.0	15.404593957708657	0.0
33	3.914415225509461E-4	0.0	0.0	15.654920811379988	0.0
34	3.914415225509461E-4	0.0	0.0	15.88352266054974	0.0
35	3.914415225509461E-4	0.0	0.0	16.16242474536729	0.0
36	3.914415225509461E-4	0.0	0.0	16.388677945401735	0.0
37	3.914415225509461E-4	0.0	0.0	16.616105470003838	0.0
38	3.914415225509461E-4	0.0	0.0	16.85116610429568	0.0
39	3.914415225509461E-4	0.0	0.0	17.091706919903235	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCG	60	0.0	45.000004	1
CGGGTAC	30	2.1631258E-6	45.000004	6
TCGTTCG	40	6.8030204E-9	45.000004	45
CGACGTC	20	7.029602E-4	45.000004	1
CGACGAT	20	7.029602E-4	45.000004	34
GCGAAGT	20	7.029602E-4	45.000004	17
GCGCGAC	30	2.1631258E-6	45.000004	9
GACGTGC	20	7.029602E-4	45.000004	39
GACGTAA	20	7.029602E-4	45.000004	42
CGCATAG	40	6.8030204E-9	45.000004	1
TGAACGT	20	7.029602E-4	45.000004	21
TGTATCG	35	1.2101555E-7	45.000004	35
CGTCTAT	20	7.029602E-4	45.000004	39
CTAAGCG	30	2.1631258E-6	45.000004	1
TGACGTA	20	7.029602E-4	45.000004	41
TACGGGT	60	0.0	45.000004	4
CACGAAC	20	7.029602E-4	45.000004	41
CGCGACG	65	0.0	45.000004	1
ACGGGTA	65	0.0	45.000004	5
ACCCTCG	20	7.029602E-4	45.000004	14
>>END_MODULE