FastQC Report![[OK]](data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAACAAAAAgCAYAAABzenr0AAAFlUlEQVR42s2XCUzTVxzHybIs0IgCLYqyQ9RtmhjCNKKbikajYVc22NThNjYjeC0yWBESxAV1Ckw8QEUF5ChnS4FyFETucwWU+7ClHKKiImLI4nCz9bv3/pS7ra1HtM1Lm/b/fp/ve+93PQMABq9y6D/Bz+ANNvethWxPw3Xs3wx/YAb9Tn6j/700AeZc1qccrhGPw2UNkk8Fx9voIceX9Tcz6Hf62/B/PPrsCxNgzjW043iyGjheRkOWQRzFt2IH/Nl4DDFyHjJ6siDoSoZ/YwB2VuyEffpGzA5iK+izdA6d+8wCyHa+SYyEsj2N/jU/aaL0vbIf0sF2tA92oOVBG+ruN6CqrwZldypR0FuMnBuXkX49C5GyaGwq3ASLU2ZKOpfaoLb0EmDibmLC8TQq5/iw/vlIaA1xjxiyQTmaH7Sitr8ekr5qlN6pQP6tImTfyIXoeiYEnSmIlSfiooyHc20R8G84htUZq0FtUFvUpk4ChldO4H6sRzvKXdEw0ISmgRZc7a/DXwRccrscebcKIb5xCWndGeB3CsGTJyBCGoPQtnCcajlLjugk/qgPxO+1h7Eqyw7UFrWpbiemCKBbRlW7lrmgcaAZV/prUXm3CsW3y3D5VgGyenKQ2p2OpM5k8NoTEC6Nxtm2MJxsPoPAxhM4XB+AA1cPwbvmADyqvPFLpQdsM1aodoIVqlUAdRp6bgv5HzBbXHFXgqLbpci9mY/MnmykdIuQ2CFATHs8wqRRONN6ASeaTyOg8TgO1fnDl4C9anzhLvHCnkp3uJTvgXOJC7YUOWN+0nww/jTJMSevvsHs+DRlSOsZFPaW4NLNPOLpYgi70pBAwFHtcbhwLRKnW8/jeHMIOecgHKw7iv1XD2Jf9X78KtmHyNZoiOQi/FiyHZsJ+Ov87/DZZUesEq8H+4SxkjLUCmDinISPY74jcm4Oe3RyVyriO/iIksUS8EWEtJ5DUFMwjhIH86s7Ap8rfvCs9oGbxBO7KtwQ3hKBrgdd6H94D3ypgIAdsD7nc6wWb8DyzDWYy58HyhifJ8ZWT5NMoLGCJ48jsZ2COHkSCSkezl+LQHBLKI40BGJLsRPW5qzDskxbLE5bDBuRDWwzV5DV2SGwNhCdAx0Yed172AdeWyyWpa/ColQbzE1+H7MTLcEOmKagrAkCaAqlWWwZf6kqlMY8+gDZ3jXZazEz0Rym8TPUDg+yetl9Kca/HikeIUUmgEXizAnPml2YDsoaSduMAJrHaSrdWvw9AYcR8LBHu0m4eJtvqRGsDS4k8DlJFlPnxM0AZVHmmABSTGg+31GxW+XRAfi51AWztKxaI/zxELNytXDVoCzKHBNAKhotKrsr9zIe7Vq+G5wEs9EJi1I+hFDKx7zkuTrAk7XCGQGERZlTBLiSuN1Ltn2BcMEEeGF3PoaI8dzObFgJ3nsuuHoBqiNwLNgMu+x1ow9SByrsyoNC+ZiBPFb8h5LrhWrhqTKhTnD1R6BywuWZn0zYejq2FTuj/k7tKEz5RPlccLVOOBKG7DATtZO+yXNATW81npD3ZHhaux5wTWE4kohoktA0cWPOelT3SkZFDMNT9ICbwCJplvpEND4Vm8ZqNrJBJWJIT7h5Ioek5a+wRLRUcyoeX4y0GaMiaEjqCn9X8A62le0ADXGLYLbmYjS+HJtGTdf5TLUNa5E1Kc8+8K09iPkJVk8vx+MbEm1H8bTBTjDFF3lfMlmVNipLREt0a0gmt2TPIsKKZEv3Ki5TU2gZt8+1168lm9yU6noc1MO3FDkxYNojhpH+YW2Gnf5Nqbq2nHHMWPVbbZNuA6firQghpZt2TRHSSGwn/eScYPNnb8s1XUxoDFtGWpD6sAgrxSvhSi4juypJ31f6ExwLSNsl/BicAOMXczF5ba5mr83l9GWN/wE1f784zfWjGwAAAABJRU5ErkJg)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | SRR3552969_1.fastq |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 545391 |
| Sequences flagged as poor quality | 0 |
| Sequence length | 51 |
| %GC | 49 |
Per base sequence quality
Per sequence quality scores
![[FAIL]](data:image/png;base64,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)
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
![[WARN]](data:image/png;base64,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)
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| CTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGCT | 4400 | 0.8067606542828907 | No Hit |
| AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2865 | 0.5253111987546549 | No Hit |
| AATGATACGGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCC | 1731 | 0.3173869755826554 | No Hit |
| AATCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCT | 1579 | 0.2895170620710646 | No Hit |
| AAACTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCT | 1506 | 0.27613216939773483 | No Hit |
| AATGATACCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGT | 1501 | 0.2752153959269588 | No Hit |
| AAAAACTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTT | 1013 | 0.18573830517922005 | No Hit |
| TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 944 | 0.1730868312825111 | No Hit |
| CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 702 | 0.1287149952969521 | No Hit |
| AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA | 563 | 0.10322869280937896 | No Hit |
Adapter Content
Kmer Content
| Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| TTGATAC | 25 | 3.8880993E-5 | 45.000004 | 27 |
| CGAGTTT | 25 | 3.8880993E-5 | 45.000004 | 42 |
| ATACTAG | 25 | 3.8880993E-5 | 45.000004 | 1 |
| CCATCGC | 25 | 3.8880993E-5 | 45.000004 | 34 |
| CGCTACG | 25 | 3.8880993E-5 | 45.000004 | 1 |
| TCTAACG | 25 | 3.8880993E-5 | 45.000004 | 1 |
| ACAATAC | 25 | 3.8880993E-5 | 45.000004 | 21 |
| ACTAACG | 25 | 3.8880993E-5 | 45.000004 | 1 |
| CATACCC | 25 | 3.8880993E-5 | 45.000004 | 15 |
| GCGATAG | 25 | 3.8880993E-5 | 45.000004 | 1 |
| ACAGACG | 50 | 2.1827873E-11 | 45.000004 | 1 |
| CGACTAA | 25 | 3.8880993E-5 | 45.000004 | 30 |
| GCGTAAC | 25 | 3.8880993E-5 | 45.000004 | 17 |
| GTCGGCC | 25 | 3.8880993E-5 | 45.000004 | 8 |
| CGAAGTA | 25 | 3.8880993E-5 | 45.000004 | 35 |
| TACCGGA | 25 | 3.8880993E-5 | 45.000004 | 45 |
| CTTACCG | 25 | 3.8880993E-5 | 45.000004 | 1 |
| ACGCGTA | 25 | 3.8880993E-5 | 45.000004 | 23 |
| CGGACTA | 25 | 3.8880993E-5 | 45.000004 | 6 |
| TTAATGT | 20 | 7.030022E-4 | 45.0 | 31 |