##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552969_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	545391
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.501601603253444	31.0	31.0	34.0	30.0	34.0
2	31.68235999493941	31.0	31.0	34.0	30.0	34.0
3	31.873078213611887	31.0	31.0	34.0	30.0	34.0
4	35.703663976853306	37.0	35.0	37.0	35.0	37.0
5	35.31708810743118	37.0	35.0	37.0	33.0	37.0
6	35.34666138605147	37.0	35.0	37.0	33.0	37.0
7	35.34005878351495	37.0	35.0	37.0	33.0	37.0
8	35.63848688372195	37.0	35.0	37.0	35.0	37.0
9	36.47631332383556	39.0	37.0	39.0	33.0	39.0
10	36.179891123982614	38.0	35.0	39.0	31.0	39.0
11	36.40197583018421	38.0	35.0	39.0	32.0	39.0
12	36.05055822336636	37.0	35.0	39.0	32.0	39.0
13	35.77690317588666	37.0	35.0	39.0	31.0	39.0
14	36.8396838231654	39.0	35.0	40.0	31.0	41.0
15	36.87598621906119	38.0	35.0	40.0	32.0	41.0
16	36.94507793491275	38.0	35.0	40.0	32.0	41.0
17	36.91087678381198	38.0	35.0	40.0	32.0	41.0
18	36.79838867894777	38.0	35.0	40.0	32.0	41.0
19	36.8276062494614	38.0	35.0	40.0	32.0	41.0
20	36.77420236124175	38.0	35.0	40.0	31.0	41.0
21	36.77024740048882	38.0	35.0	40.0	31.0	41.0
22	36.75666081765192	38.0	35.0	40.0	31.0	41.0
23	36.699600836830825	38.0	35.0	40.0	31.0	41.0
24	36.3371929496453	38.0	35.0	40.0	31.0	41.0
25	36.4512157332996	38.0	35.0	40.0	31.0	41.0
26	36.367054095135416	38.0	35.0	40.0	31.0	41.0
27	36.27405476071296	38.0	35.0	40.0	30.0	41.0
28	36.246315029034214	38.0	35.0	40.0	30.0	41.0
29	36.01295584268901	38.0	35.0	40.0	30.0	41.0
30	35.95630290928893	38.0	35.0	40.0	30.0	41.0
31	35.80260033627251	38.0	34.0	40.0	30.0	41.0
32	35.75596590336107	38.0	34.0	40.0	30.0	41.0
33	35.770351912664495	38.0	34.0	40.0	30.0	41.0
34	35.62225999328922	38.0	34.0	40.0	29.0	41.0
35	35.555661901278164	38.0	34.0	40.0	29.0	41.0
36	35.290835382322044	37.0	34.0	40.0	28.0	41.0
37	35.10925372805932	37.0	34.0	40.0	27.0	41.0
38	35.096316220839725	37.0	34.0	40.0	27.0	41.0
39	35.03227592681214	37.0	34.0	40.0	27.0	41.0
40	34.87392531229888	37.0	34.0	40.0	26.0	41.0
41	34.80512696395797	37.0	34.0	40.0	26.0	41.0
42	34.755667035209605	37.0	33.0	40.0	26.0	41.0
43	34.64228415943791	37.0	33.0	40.0	26.0	41.0
44	34.51709507490956	36.0	33.0	40.0	25.0	41.0
45	34.26293613205939	36.0	33.0	40.0	24.0	41.0
46	34.16309583399799	36.0	33.0	39.0	24.0	41.0
47	34.024565861922916	35.0	33.0	39.0	24.0	41.0
48	33.96572550702157	35.0	33.0	39.0	23.0	41.0
49	33.777964799565815	35.0	33.0	39.0	23.0	40.0
50	33.55890727936471	35.0	33.0	39.0	23.0	40.0
51	31.638015662157976	34.0	29.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	3.0
10	10.0
11	14.0
12	11.0
13	13.0
14	31.0
15	46.0
16	74.0
17	220.0
18	423.0
19	719.0
20	1145.0
21	1780.0
22	2363.0
23	3268.0
24	4087.0
25	4772.0
26	5703.0
27	7133.0
28	8833.0
29	11249.0
30	14277.0
31	18237.0
32	23902.0
33	33606.0
34	56201.0
35	60910.0
36	55239.0
37	74249.0
38	92622.0
39	64248.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.657839971690034	32.505486889222595	20.266010990280368	26.570662148807
2	24.082905658509215	31.86924610050404	20.059736959355764	23.988111281630978
3	20.813508107027804	31.619883716452968	20.552777731939102	27.01383044458013
4	21.538125858329163	32.00547863826136	19.628670073396883	26.827725430012595
5	16.9153873092882	36.07026885298804	18.374340610681145	28.640003227042616
6	21.88686648661236	37.64125187250981	19.231890515245027	21.239991125632805
7	80.84731871263003	7.633239272375232	6.276047826238424	5.243394188756324
8	83.60038944537038	5.832146111688679	4.930957789915859	5.636506653025077
9	76.12300166302708	10.35440628833259	8.867583073428055	4.655008975212279
10	43.88447920849446	29.207669360147126	11.831144995058592	15.076706436299828
11	35.94302069524433	25.61391735470516	21.200386511695278	17.242675438355235
12	32.483117616535665	24.484635793403264	25.19220155814819	17.840045031912886
13	24.51800634773951	32.88943161878359	22.449031978892208	20.143530054584694
14	19.122977826916838	33.529889565467705	28.090122499271164	19.25701010834429
15	17.08242343566359	28.418877465891445	32.28582796562466	22.212871132820307
16	19.33640269091349	25.631886114732367	31.566160791065496	23.46555040328865
17	18.408077874405702	26.273810898969728	28.091589336824406	27.22652188980016
18	23.954190663212263	25.713295598937275	26.01858116470569	24.313932573144772
19	24.779286786910674	29.78376980918277	23.982060576723853	21.4548828271827
20	27.496603354290777	28.079487927010167	22.853879143586894	21.57002957511217
21	22.75541767282555	30.257008274797347	24.31429928253308	22.67327476984402
22	22.672541351067398	27.37595596553665	23.69181009587617	26.25969258751978
23	23.428696109763454	29.56411088558484	21.20827076354395	25.798922241107757
24	19.645171995870854	29.226188184256802	27.48083485059343	23.647804969278923
25	20.985494810145383	27.462132671789597	26.820391242246387	24.731981275818633
26	24.10839196099679	30.810006032369436	22.67749192780959	22.404110078824182
27	21.370172958482996	29.415043519236654	24.355370734023847	24.8594127882565
28	21.907952276440206	27.95682363662033	29.272943631266372	20.862280455673083
29	22.751933933636604	28.100940426226323	27.06498640424943	22.08213923588765
30	24.08895636341634	29.259192029204733	24.763334928519175	21.888516678859755
31	29.604265563604827	28.244873861138153	20.669941381504277	21.48091919375274
32	28.35580345110205	28.09140598213025	22.121193785742705	21.43159678102499
33	27.849377785845387	26.18745083802263	23.433463331811488	22.529708044320497
34	23.524957324194936	27.959023892950196	23.98261064080632	24.53340814204855
35	25.45623231773168	27.42344483132285	26.379423202803125	20.740899648142342
36	29.640753147741712	28.14036168546969	23.91385263049812	18.305032536290476
37	24.68614260227983	32.14262794948945	21.205520443131626	21.965709005099093
38	26.46193281517297	29.419810741284692	22.93840565759244	21.179850785949895
39	25.930754266205348	27.236973107367007	23.25872630828158	23.573546318146064
40	27.23972342777934	27.16509806725817	21.489903573766345	24.105274931196153
41	21.543443144459662	25.29322999462771	27.58241335115541	25.58091350975722
42	26.27289412549895	26.359437541140206	22.932354952685323	24.435313380675517
43	24.066953800117712	25.286445870943965	25.365288389430702	25.281311939507617
44	24.617201237277474	24.327500820512256	24.097390679347477	26.95790726286279
45	25.337601830613266	27.91758573207112	23.937872095432454	22.806940341883163
46	23.41274425137195	26.267210129980146	27.72451323912569	22.595532379522215
47	23.541825956057213	26.263176326708727	25.194218459783897	25.000779257450162
48	23.940072351762314	23.75671765760711	29.88039773300256	22.422812257628014
49	24.099774290371496	23.565662066297392	27.785570352279375	24.54899329105174
50	22.524574112884153	26.14692945061433	25.140128825008112	26.18836761149341
51	22.282912625987596	25.409476870722102	27.375772610842496	24.931837892447803
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3365.0
1	2646.5
2	1928.0
3	1357.0
4	786.0
5	718.0
6	650.0
7	666.0
8	682.0
9	741.0
10	800.0
11	794.0
12	788.0
13	893.0
14	998.0
15	1005.0
16	1012.0
17	1027.5
18	1043.0
19	1160.5
20	1278.0
21	1289.5
22	1301.0
23	1820.5
24	2340.0
25	2072.0
26	2185.5
27	2567.0
28	3406.5
29	4246.0
30	5059.5
31	5873.0
32	7062.5
33	8252.0
34	8559.0
35	8866.0
36	9744.5
37	10623.0
38	12414.5
39	14206.0
40	17135.0
41	20064.0
42	26176.5
43	32289.0
44	41336.5
45	50384.0
46	50763.5
47	51143.0
48	51428.0
49	51713.0
50	49084.5
51	46456.0
52	43407.0
53	40358.0
54	37542.5
55	34727.0
56	31190.5
57	27654.0
58	26097.5
59	24541.0
60	23877.5
61	23214.0
62	20905.0
63	18596.0
64	16284.5
65	13973.0
66	12859.5
67	11746.0
68	10238.5
69	8731.0
70	7184.5
71	5638.0
72	5013.5
73	4389.0
74	3360.5
75	2125.0
76	1918.0
77	1466.0
78	1014.0
79	745.0
80	476.0
81	405.0
82	334.0
83	249.5
84	165.0
85	118.5
86	72.0
87	49.5
88	27.0
89	22.0
90	17.0
91	11.5
92	6.0
93	5.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	545391.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.112236648539543
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.32574639324143	21.235861718870545
2	9.706104831710752	5.2630842226575005
3	3.3520719762554068	2.726465060495227
4	1.5715727982613237	1.7043541446747401
5	0.8725368717123615	1.1828213075220966
6	0.5456362790893484	0.8876051953619389
7	0.35100322004887746	0.6661537656455196
8	0.288532168723274	0.6258201951313395
9	0.2492555295293291	0.6082087412300587
>10	2.899262244926606	19.93727855455048
>50	1.45468410607048	28.689023305527332
>100	0.3754894907034294	13.207002910474433
>500	0.003376704053088394	0.5939763959736457
>1k	0.004727385674323751	2.672344481885129
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGCT	4400	0.8067606542828907	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2865	0.5253111987546549	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCC	1731	0.3173869755826554	No Hit
AATCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCT	1579	0.2895170620710646	No Hit
AAACTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCT	1506	0.27613216939773483	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGT	1501	0.2752153959269588	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTT	1013	0.18573830517922005	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	944	0.1730868312825111	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	702	0.1287149952969521	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	563	0.10322869280937896	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8335469415520241E-4	0.0	0.0	1.1279980784428052	0.0
2	1.8335469415520241E-4	0.0	0.0	1.410364307441817	0.0
3	1.8335469415520241E-4	0.0	0.0	1.7662557687970648	0.0
4	1.8335469415520241E-4	0.0	0.0	3.689463155790983	0.0
5	1.8335469415520241E-4	0.0	0.0	3.971279320707529	0.0
6	1.8335469415520241E-4	0.0	0.0	5.29895066108535	0.0
7	1.8335469415520241E-4	0.0	0.0	6.32316998263631	0.0
8	1.8335469415520241E-4	0.0	0.0	6.821894750738461	0.0
9	1.8335469415520241E-4	0.0	0.0	8.00783291253431	0.0
10	1.8335469415520241E-4	0.0	0.0	9.210823060886593	0.0
11	1.8335469415520241E-4	0.0	0.0	10.903553597327422	0.0
12	3.6670938831040483E-4	0.0	0.0	11.600668144505502	0.0
13	3.6670938831040483E-4	0.0	0.0	11.932723495620573	0.0
14	3.6670938831040483E-4	0.0	0.0	12.291915341470615	0.0
15	3.6670938831040483E-4	0.0	0.0	12.569514348421592	0.0
16	3.6670938831040483E-4	0.0	0.0	13.100692897389212	0.0
17	3.6670938831040483E-4	0.0	0.0	13.754535736746664	0.0
18	3.6670938831040483E-4	0.0	0.0	14.547361434273759	0.0
19	3.6670938831040483E-4	0.0	0.0	14.899219092357592	0.0
20	3.6670938831040483E-4	0.0	0.0	15.230541024696043	0.0
21	3.6670938831040483E-4	0.0	0.0	15.638138509803058	0.0
22	3.6670938831040483E-4	0.0	0.0	16.046836123075003	0.0
23	3.6670938831040483E-4	0.0	0.0	16.495688414366942	0.0
24	3.6670938831040483E-4	0.0	0.0	16.807758103819094	0.0
25	3.6670938831040483E-4	0.0	0.0	17.06738835074286	0.0
26	3.6670938831040483E-4	0.0	0.0	17.311433448663436	0.0
27	3.6670938831040483E-4	0.0	0.0	17.567579956398255	0.0
28	3.6670938831040483E-4	0.0	0.0	17.821709562497364	0.0
29	3.6670938831040483E-4	0.0	0.0	18.09509141148277	0.0
30	3.6670938831040483E-4	0.0	0.0	18.479769559820387	0.0
31	3.6670938831040483E-4	0.0	0.0	18.755351665135656	0.0
32	3.6670938831040483E-4	0.0	0.0	18.995729669173127	0.0
33	3.6670938831040483E-4	0.0	0.0	19.27607899653643	0.0
34	3.6670938831040483E-4	0.0	0.0	19.526541508752437	0.0
35	3.6670938831040483E-4	0.0	0.0	19.82339275858971	0.0
36	3.6670938831040483E-4	0.0	0.0	20.06065373282654	0.0
37	3.6670938831040483E-4	0.0	0.0	20.311849663819167	0.0
38	3.6670938831040483E-4	0.0	0.0	20.57478029523773	0.0
39	3.6670938831040483E-4	0.0	0.0	20.845961887893274	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATAC	25	3.8880993E-5	45.000004	27
CGAGTTT	25	3.8880993E-5	45.000004	42
ATACTAG	25	3.8880993E-5	45.000004	1
CCATCGC	25	3.8880993E-5	45.000004	34
CGCTACG	25	3.8880993E-5	45.000004	1
TCTAACG	25	3.8880993E-5	45.000004	1
ACAATAC	25	3.8880993E-5	45.000004	21
ACTAACG	25	3.8880993E-5	45.000004	1
CATACCC	25	3.8880993E-5	45.000004	15
GCGATAG	25	3.8880993E-5	45.000004	1
ACAGACG	50	2.1827873E-11	45.000004	1
CGACTAA	25	3.8880993E-5	45.000004	30
GCGTAAC	25	3.8880993E-5	45.000004	17
GTCGGCC	25	3.8880993E-5	45.000004	8
CGAAGTA	25	3.8880993E-5	45.000004	35
TACCGGA	25	3.8880993E-5	45.000004	45
CTTACCG	25	3.8880993E-5	45.000004	1
ACGCGTA	25	3.8880993E-5	45.000004	23
CGGACTA	25	3.8880993E-5	45.000004	6
TTAATGT	20	7.030022E-4	45.0	31
>>END_MODULE