FastQCFastQC Report
Sat 18 Jun 2016
SRR3552966_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552966_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440218
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCC39800.9040975153219541No Hit
CTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGCT36610.8316334179883602No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGT31600.7178261679440641No Hit
AATCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCT31240.7096484014738152No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26010.5908436274754781No Hit
AAACTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCT8770.1992194776224507No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG8120.18445406594005698No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA7780.1767306198292664No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7090.16105656742795615No Hit
AATGACTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTT6850.15560472311445692No Hit
AAAAACTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTT6200.1408393114320632No Hit
CCGCAGAGAGGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAG5590.126982540468586No Hit
AATGATCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCT5310.12062205543617026No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4820.1094912066294427No Hit
AACTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTG4600.10449368267540173No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA4430.10063195962000644No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCAAG302.1624382E-645.0000041
ATCAAGT302.1624382E-645.00000445
CTACGAA302.1624382E-645.00000410
TATTCCG302.1624382E-645.0000041
GCTACGA302.1624382E-645.0000049
TAGTACG302.1624382E-645.0000041
AGGTATA207.0285384E-445.011
AATCCGG800.045.02
TCGTTGC207.0285384E-445.039
ACACGTT207.0285384E-445.033
CTCCGTG207.0285384E-445.043
CGAGTTT253.8868704E-545.028
CGAGTAG253.8868704E-545.01
CGAAACG207.0285384E-445.01
CCGATAT207.0285384E-445.019
CGCATAG253.8868704E-545.01
TAGCGCG207.0285384E-445.01
ATTACCG253.8868704E-545.01
ATTACAC207.0285384E-445.018
TTGTCGT207.0285384E-445.043