##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552966_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	440218
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1774575324044	31.0	31.0	34.0	28.0	34.0
2	31.40831360825773	31.0	31.0	34.0	28.0	34.0
3	31.353879214389234	31.0	31.0	34.0	28.0	34.0
4	35.38089083136082	37.0	35.0	37.0	33.0	37.0
5	35.04920516653113	37.0	35.0	37.0	32.0	37.0
6	35.08979187584333	37.0	35.0	37.0	32.0	37.0
7	35.0044455247173	37.0	35.0	37.0	32.0	37.0
8	35.377962736644115	37.0	35.0	37.0	33.0	37.0
9	35.84509038703551	39.0	37.0	39.0	32.0	39.0
10	35.73169202531473	38.0	35.0	39.0	30.0	39.0
11	36.11510660627235	38.0	35.0	39.0	31.0	39.0
12	35.8105484101059	37.0	35.0	39.0	31.0	39.0
13	35.5588753753822	37.0	35.0	39.0	30.0	39.0
14	36.554052764766546	38.0	35.0	40.0	31.0	41.0
15	36.62254610215847	38.0	35.0	40.0	31.0	41.0
16	36.67598099123616	38.0	35.0	40.0	31.0	41.0
17	36.637459167957694	38.0	35.0	40.0	31.0	41.0
18	36.50471130212758	38.0	35.0	40.0	31.0	41.0
19	36.54852368599194	38.0	35.0	40.0	31.0	41.0
20	36.473597172310086	38.0	35.0	40.0	31.0	41.0
21	36.5124143038222	38.0	35.0	40.0	31.0	41.0
22	36.51424748647261	38.0	35.0	40.0	31.0	41.0
23	36.451778437047096	38.0	35.0	40.0	31.0	41.0
24	36.013909017804814	38.0	34.0	40.0	30.0	41.0
25	36.16977270352416	38.0	35.0	40.0	30.0	41.0
26	35.9721592483724	38.0	34.0	40.0	30.0	41.0
27	35.90128300069511	38.0	34.0	40.0	30.0	41.0
28	35.79705509542999	38.0	34.0	40.0	29.0	41.0
29	35.507278212158525	38.0	34.0	40.0	29.0	41.0
30	35.61359144787355	38.0	34.0	40.0	29.0	41.0
31	35.431268144419356	38.0	34.0	40.0	29.0	41.0
32	35.35497185485373	37.0	34.0	40.0	28.0	41.0
33	35.35494005242857	38.0	34.0	40.0	28.0	41.0
34	35.25508952382683	37.0	34.0	40.0	27.0	41.0
35	35.15233134492456	37.0	34.0	40.0	27.0	41.0
36	34.86433312586037	37.0	33.0	40.0	26.0	41.0
37	34.73554239036114	37.0	33.0	40.0	26.0	41.0
38	34.66946603728153	37.0	33.0	40.0	25.0	41.0
39	34.5983717158317	37.0	33.0	40.0	25.0	41.0
40	34.42487812856358	37.0	33.0	40.0	24.0	41.0
41	34.474928330963294	37.0	33.0	40.0	25.0	41.0
42	34.45158307929253	37.0	33.0	40.0	25.0	41.0
43	34.35596908804274	36.0	33.0	40.0	25.0	41.0
44	34.35954004606808	36.0	33.0	40.0	25.0	41.0
45	34.12114225224775	36.0	33.0	40.0	24.0	41.0
46	33.96788863699349	36.0	33.0	39.0	23.0	41.0
47	33.859717231008275	35.0	33.0	39.0	23.0	41.0
48	33.78428187852382	35.0	32.0	39.0	23.0	41.0
49	33.64090745948598	35.0	32.0	39.0	23.0	40.0
50	33.550020671576355	35.0	32.0	39.0	23.0	40.0
51	31.63278421145887	34.0	29.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	6.0
11	9.0
12	5.0
13	22.0
14	33.0
15	42.0
16	93.0
17	182.0
18	389.0
19	653.0
20	1091.0
21	1640.0
22	2244.0
23	2898.0
24	3540.0
25	4388.0
26	5142.0
27	6488.0
28	8245.0
29	10394.0
30	13386.0
31	16937.0
32	21641.0
33	30000.0
34	46418.0
35	48585.0
36	45882.0
37	58060.0
38	67431.0
39	44365.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.997446719579845	35.664148217474064	20.350599021393943	25.98780604155214
2	23.0363138263315	34.61171510478899	19.078729175090523	23.273241893788988
3	20.3260657219832	31.950760759441916	22.354378966784637	25.36879455179025
4	21.909145014515534	31.97234097651618	19.20775615717667	26.91075785179161
5	16.79372492719516	37.441449463674815	17.74166435720484	28.02316125192518
6	20.0087229509016	38.53817881140708	20.33038176539805	21.122716472293273
7	78.78142193186103	9.777655616081123	6.268939479984917	5.171982972072928
8	81.27064318133289	6.107882912556961	4.997296793861223	7.6241771122489315
9	73.64669322926368	11.906827980682298	8.681153428528592	5.765325361525426
10	43.826467795501316	27.256949965698812	12.352970573670317	16.56361166512955
11	34.337532767855926	25.37560935718212	21.935949915723572	18.350907959238377
12	33.32757860878019	22.566319414471923	26.66996806127873	17.436133915469153
13	24.873585359980737	31.364460335561017	22.963395408638448	20.7985588958198
14	19.228654893711752	31.58934891349286	29.88451176462571	19.29748442816968
15	17.706227369167095	28.786646615994805	30.887651118309567	22.619474896528537
16	19.582797613909474	24.270247922620154	32.755816436401965	23.391138027068408
17	18.605781680894466	26.342403082109318	27.438905269661852	27.612909967334364
18	20.985284563557148	26.917118336824032	26.71835317956103	25.379243920057785
19	22.94681271551822	30.665034142175013	25.790176685187795	20.59797645711897
20	25.962136941242747	28.99495250080642	24.340894738515917	20.702015819434916
21	21.815327860287404	29.08967829575347	25.391510569763163	23.703483274195968
22	20.636366527493198	27.453670681344246	24.4967266218101	27.413236169352455
23	21.99205848011667	27.490016310100906	22.002280688204483	28.515644521577943
24	19.317247363806114	31.38127018886097	26.264032820102766	23.037449627230146
25	21.804651331840134	26.486649796237323	25.714305185158263	25.994393686764283
26	23.08606190569218	32.275145496095114	21.76580694110645	22.872985657106252
27	19.584842055527034	29.036977134056308	25.480330200037255	25.897850610379404
28	23.159207483564963	28.791416979769114	26.17248726767193	21.876888268993998
29	19.561671717194663	31.99096811125397	25.528033837780374	22.919326333770996
30	22.951355919112803	27.987951424067166	23.121726053909654	25.938966602910373
31	26.813987615227003	31.11503845821843	18.729129658487388	23.341844268067184
32	24.445161261011588	30.132343520710194	20.491211172646278	24.931284045631937
33	25.582552280915365	27.87346269348368	21.001185776138186	25.542799249462767
34	19.032615658605508	30.704105693088422	21.39599016850742	28.867288479798646
35	23.019276812851814	28.045650109718366	23.39840715281974	25.53666592461008
36	26.0350553589358	29.18281396944241	23.076748338323284	21.705382333298502
37	20.382174286376294	33.03045309369449	20.334470648633175	26.25290197129604
38	21.81282909831038	30.65799217660341	22.864580730456275	24.664597994629933
39	20.552998741532605	28.342775624804073	24.835195289606514	26.26903034405681
40	22.46432449377354	27.01070833087243	21.737866238999768	28.78710093635426
41	20.283813928553577	23.72074744785538	26.908031929634863	29.087406693956176
42	23.644421627466393	26.554343529796604	22.575632981840815	27.22560186089619
43	21.555229454497542	25.13254796487195	25.988260361911596	27.323962218718904
44	21.404849415516857	24.517171037985726	24.79135337491879	29.28662617157863
45	23.352747956694184	29.12011775983717	24.13531477586105	23.391819507607593
46	21.470725867638308	25.70953482138395	29.44495681684983	23.37478249412791
47	21.81555502046713	27.395290515153853	23.88066821438469	26.908486249994322
48	21.938221517520866	23.241439468626908	30.75589821406667	24.06444079978556
49	22.21013225265664	23.790258462852496	28.022706931565722	25.97690235292514
50	21.62723923147168	24.43652917418188	24.93809885102381	28.998132743322625
51	21.19200032711066	25.049634499270816	28.681017132420756	25.077348041197773
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3050.0
1	2504.0
2	1958.0
3	1326.0
4	694.0
5	612.5
6	531.0
7	543.0
8	555.0
9	595.0
10	635.0
11	685.5
12	736.0
13	783.5
14	831.0
15	951.0
16	1071.0
17	1028.0
18	985.0
19	1010.5
20	1036.0
21	973.0
22	910.0
23	1352.5
24	1795.0
25	1710.0
26	1905.0
27	2185.0
28	2749.5
29	3314.0
30	3786.5
31	4259.0
32	5166.5
33	6074.0
34	6867.0
35	7660.0
36	8764.0
37	9868.0
38	10282.0
39	10696.0
40	12447.5
41	14199.0
42	19919.0
43	25639.0
44	34521.0
45	43403.0
46	45358.0
47	47313.0
48	45883.0
49	44453.0
50	41491.0
51	38529.0
52	34592.0
53	30655.0
54	27448.5
55	24242.0
56	22531.5
57	20821.0
58	19632.0
59	18443.0
60	17694.5
61	16946.0
62	15483.0
63	14020.0
64	12642.0
65	11264.0
66	10332.5
67	9401.0
68	8189.5
69	6978.0
70	6122.0
71	5266.0
72	4237.0
73	3208.0
74	2667.5
75	1713.0
76	1299.0
77	968.5
78	638.0
79	487.5
80	337.0
81	286.5
82	236.0
83	196.0
84	156.0
85	103.5
86	51.0
87	49.0
88	47.0
89	47.5
90	48.0
91	31.5
92	15.0
93	11.0
94	7.0
95	6.0
96	5.0
97	4.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	440218.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.355669109709645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.41993677322765	24.108453214021598
2	8.988023896402819	5.4567495869873435
3	3.0797300808602914	2.80461801845443
4	1.3341011092970734	1.6199012733087417
5	0.7399555235925236	1.1230922515038295
6	0.5198660305185141	0.9468528722278937
7	0.3518740219462512	0.7476959964952178
8	0.3196268663082954	0.7761989913782413
9	0.24101758547074825	0.6584625066754076
>10	3.719971262512995	29.025317758680576
>50	1.1968271611936778	23.834300541994992
>100	0.0793393865456722	3.877751473772686
>500	0.005987878229862053	1.2657733321841937
>1k	0.0037424238936637835	3.754832182314842
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCC	3980	0.9040975153219541	No Hit
CTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGCT	3661	0.8316334179883602	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGT	3160	0.7178261679440641	No Hit
AATCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCT	3124	0.7096484014738152	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2601	0.5908436274754781	No Hit
AAACTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCT	877	0.1992194776224507	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	812	0.18445406594005698	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	778	0.1767306198292664	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	709	0.16105656742795615	No Hit
AATGACTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTT	685	0.15560472311445692	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTT	620	0.1408393114320632	No Hit
CCGCAGAGAGGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAG	559	0.126982540468586	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCT	531	0.12062205543617026	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	482	0.1094912066294427	No Hit
AACTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTG	460	0.10449368267540173	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	443	0.10063195962000644	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.271601797291342E-4	0.0	0.0	1.13080337469163	0.0
2	2.271601797291342E-4	0.0	0.0	1.3500129481302445	0.0
3	2.271601797291342E-4	0.0	0.0	1.745044500679209	0.0
4	2.271601797291342E-4	0.0	0.0	4.040043796482652	0.0
5	2.271601797291342E-4	0.0	0.0	4.298552081014407	0.0
6	2.271601797291342E-4	0.0	0.0	5.652881072559505	0.0
7	2.271601797291342E-4	0.0	0.0	6.709403068479708	0.0
8	2.271601797291342E-4	0.0	0.0	7.186212285731161	0.0
9	2.271601797291342E-4	0.0	0.0	9.005311005002067	0.0
10	2.271601797291342E-4	0.0	0.0	10.25900803692716	0.0
11	2.271601797291342E-4	0.0	0.0	12.859764934646016	0.0
12	2.271601797291342E-4	0.0	0.0	13.570776297198206	0.0
13	2.271601797291342E-4	0.0	0.0	13.867901812283915	0.0
14	2.271601797291342E-4	0.0	0.0	14.276335815436898	0.0
15	2.271601797291342E-4	0.0	0.0	14.578231694296916	0.0
16	2.271601797291342E-4	0.0	0.0	15.152946949011627	0.0
17	2.271601797291342E-4	0.0	0.0	15.787405330995098	0.0
18	2.271601797291342E-4	0.0	0.0	16.593823969033526	0.0
19	2.271601797291342E-4	0.0	0.0	16.966593823969035	0.0
20	2.271601797291342E-4	0.0	0.0	17.335274795669417	0.0
21	2.271601797291342E-4	0.0	0.0	17.76483469553721	0.0
22	2.271601797291342E-4	0.0	0.0	18.19802915828067	0.0
23	2.271601797291342E-4	0.0	0.0	18.68801366595641	0.0
24	2.271601797291342E-4	0.0	0.0	19.025119372674446	0.0
25	2.271601797291342E-4	0.0	0.0	19.31588440272774	0.0
26	2.271601797291342E-4	0.0	0.0	19.573711206720308	0.0
27	2.271601797291342E-4	0.0	0.0	19.83244665143179	0.0
28	2.271601797291342E-4	0.0	0.0	20.1007228236919	0.0
29	2.271601797291342E-4	0.0	0.0	20.39103353338573	0.0
30	2.271601797291342E-4	0.0	0.0	20.71950715327406	0.0
31	2.271601797291342E-4	0.0	0.0	21.00618330009223	0.0
32	2.271601797291342E-4	0.0	0.0	21.268780467859106	0.0
33	2.271601797291342E-4	0.0	0.0	21.559772658092125	0.0
34	2.271601797291342E-4	0.0	0.0	21.801925409683385	0.0
35	2.271601797291342E-4	0.0	0.0	22.14675456251221	0.0
36	2.271601797291342E-4	0.0	0.0	22.409578890458818	0.0
37	2.271601797291342E-4	0.0	0.0	22.685578508829717	0.0
38	2.271601797291342E-4	0.0	0.0	22.964076889177633	0.0
39	2.271601797291342E-4	0.0	0.0	23.228718498562078	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTCAAG	30	2.1624382E-6	45.000004	1
ATCAAGT	30	2.1624382E-6	45.000004	45
CTACGAA	30	2.1624382E-6	45.000004	10
TATTCCG	30	2.1624382E-6	45.000004	1
GCTACGA	30	2.1624382E-6	45.000004	9
TAGTACG	30	2.1624382E-6	45.000004	1
AGGTATA	20	7.0285384E-4	45.0	11
AATCCGG	80	0.0	45.0	2
TCGTTGC	20	7.0285384E-4	45.0	39
ACACGTT	20	7.0285384E-4	45.0	33
CTCCGTG	20	7.0285384E-4	45.0	43
CGAGTTT	25	3.8868704E-5	45.0	28
CGAGTAG	25	3.8868704E-5	45.0	1
CGAAACG	20	7.0285384E-4	45.0	1
CCGATAT	20	7.0285384E-4	45.0	19
CGCATAG	25	3.8868704E-5	45.0	1
TAGCGCG	20	7.0285384E-4	45.0	1
ATTACCG	25	3.8868704E-5	45.0	1
ATTACAC	20	7.0285384E-4	45.0	18
TTGTCGT	20	7.0285384E-4	45.0	43
>>END_MODULE