SRR3552964_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552964_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	330044
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGCT	2197	0.6656688199149204	RNA PCR Primer, Index 17 (96% over 25bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCC	1347	0.4081274011949922	No Hit
AATCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCT	1337	0.4050975021512283	TruSeq Adapter, Index 9 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1301	0.3941898655936784	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGT	1234	0.37388954200046054	No Hit
AAACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCT	840	0.2545115196761644	RNA PCR Primer, Index 47 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTT	677	0.20512416526281343	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	418	0.12664978002932942	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	393	0.11907503241991976	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCT	359	0.10877337567112265	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGATC	20	7.025969E-4	45.000004	43
TTTAGAC	20	7.025969E-4	45.000004	34
GAGTGTA	20	7.025969E-4	45.000004	45
GGAATAC	20	7.025969E-4	45.000004	41
AGTCCGA	20	7.025969E-4	45.000004	11
GGTACAG	20	7.025969E-4	45.000004	1
TCCGCAT	25	3.8847415E-5	45.000004	31
CGTATCG	20	7.025969E-4	45.000004	1
GTCGAAA	20	7.025969E-4	45.000004	26
CTCAAAC	20	7.025969E-4	45.000004	22
CATGCGA	20	7.025969E-4	45.000004	36
CTATCGT	20	7.025969E-4	45.000004	19
ACGTTAA	20	7.025969E-4	45.000004	11
CGTGCCG	20	7.025969E-4	45.000004	13
GACACCC	20	7.025969E-4	45.000004	11
ACCGCGA	25	3.8847415E-5	45.000004	36
CGCATGA	25	3.8847415E-5	45.000004	33
CGCATCC	25	3.8847415E-5	45.000004	26
CCAATCG	20	7.025969E-4	45.000004	1
CACGCCC	30	2.160783E-6	45.000004	13