##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552964_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	330044
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.204263673934385	31.0	31.0	34.0	28.0	34.0
2	31.42997297330053	31.0	31.0	34.0	28.0	34.0
3	31.41178751923986	31.0	31.0	34.0	28.0	34.0
4	35.421098399001345	37.0	35.0	37.0	33.0	37.0
5	35.0720782683521	37.0	35.0	37.0	32.0	37.0
6	35.13956320975385	37.0	35.0	37.0	32.0	37.0
7	35.13254293366945	37.0	35.0	37.0	32.0	37.0
8	35.44539818933233	37.0	35.0	37.0	33.0	37.0
9	35.897189465647	39.0	37.0	39.0	32.0	39.0
10	35.747136745403644	38.0	35.0	39.0	30.0	39.0
11	36.15371283828823	38.0	35.0	39.0	31.0	39.0
12	35.84268461174874	37.0	35.0	39.0	31.0	39.0
13	35.48758347371866	37.0	35.0	39.0	30.0	39.0
14	36.534159081819396	38.0	35.0	40.0	31.0	41.0
15	36.640666093005784	38.0	35.0	40.0	31.0	41.0
16	36.72509119996122	38.0	35.0	40.0	31.0	41.0
17	36.68480869217438	38.0	35.0	40.0	31.0	41.0
18	36.569048369308334	38.0	35.0	40.0	31.0	41.0
19	36.602165165856675	38.0	35.0	40.0	31.0	41.0
20	36.55115378555587	38.0	35.0	40.0	31.0	41.0
21	36.54217013489111	38.0	35.0	40.0	31.0	41.0
22	36.54790876367999	38.0	35.0	40.0	31.0	41.0
23	36.50383282229036	38.0	35.0	40.0	31.0	41.0
24	36.127764782877435	38.0	35.0	40.0	30.0	41.0
25	36.24147689398989	38.0	35.0	40.0	30.0	41.0
26	36.09507823199331	38.0	35.0	40.0	30.0	41.0
27	36.01290736992643	38.0	34.0	40.0	30.0	41.0
28	35.907509301790064	38.0	34.0	40.0	30.0	41.0
29	35.694368023657454	38.0	34.0	40.0	29.0	41.0
30	35.71779823296288	38.0	34.0	40.0	29.0	41.0
31	35.56244015949389	38.0	34.0	40.0	29.0	41.0
32	35.56518827792658	38.0	34.0	40.0	29.0	41.0
33	35.37794354692102	37.0	34.0	40.0	28.0	41.0
34	35.39100241179964	37.0	34.0	40.0	29.0	41.0
35	35.32620802074875	37.0	34.0	40.0	28.0	41.0
36	35.025239059034554	37.0	34.0	40.0	27.0	41.0
37	34.90975748688054	37.0	33.0	40.0	26.0	41.0
38	34.81490649731551	37.0	33.0	40.0	26.0	41.0
39	34.73691386602999	37.0	33.0	40.0	26.0	41.0
40	34.57076329216711	37.0	33.0	40.0	25.0	41.0
41	34.627031547308846	37.0	33.0	40.0	26.0	41.0
42	34.61130940117075	36.0	33.0	40.0	26.0	41.0
43	34.491401146513795	36.0	33.0	40.0	26.0	41.0
44	34.410269539818934	36.0	33.0	40.0	25.0	41.0
45	34.2148016628086	36.0	33.0	40.0	24.0	41.0
46	34.05481390360073	36.0	33.0	39.0	24.0	41.0
47	33.925661426961256	35.0	33.0	39.0	23.0	41.0
48	33.87378652543297	35.0	33.0	39.0	23.0	41.0
49	33.68253323799251	35.0	33.0	39.0	23.0	40.0
50	33.566224503399546	35.0	32.0	39.0	23.0	40.0
51	31.699946067797022	34.0	29.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	5.0
12	8.0
13	15.0
14	23.0
15	38.0
16	69.0
17	158.0
18	329.0
19	503.0
20	786.0
21	1239.0
22	1636.0
23	2117.0
24	2658.0
25	3100.0
26	3870.0
27	4762.0
28	6020.0
29	7345.0
30	9282.0
31	11912.0
32	15609.0
33	21532.0
34	34929.0
35	37162.0
36	34128.0
37	44652.0
38	52079.0
39	34070.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.43528741622329	32.54232768964138	20.503629819054428	27.518755075080897
2	22.781204930251725	31.588515470664518	21.102943849910922	24.52733574917284
3	20.40303717080147	31.62548023899844	20.280326259529033	27.691156330671063
4	20.144586782368414	33.13770285174098	19.783119826447383	26.934590539443228
5	17.33193150004242	36.89811055495631	18.030626219534366	27.739331725466908
6	20.999321302614195	37.395922967846715	19.372265516113004	22.23249021342609
7	82.14359297548206	7.205099926070463	5.803771618329677	4.847535480117802
8	84.09151507071785	5.37594987335022	4.693919598598974	5.838615457332962
9	76.02743876574033	11.169722824835477	7.924094969155628	4.87874344026857
10	44.57526875204518	28.22381258256475	12.05566530523203	15.14525336015804
11	35.99974548848032	24.23040564288398	21.491073917417072	18.278774951218626
12	33.96607725030602	23.194180169916738	25.39297790597617	17.44676467380107
13	24.33342221037195	33.54704221255348	22.558810340439457	19.560725236635115
14	18.17121353516501	34.19756153724958	28.17260728872514	19.45861763886027
15	15.803650422367927	28.144429227618133	34.395716934711736	21.6562034153022
16	19.033522803020205	23.704112178982196	33.70338500321169	23.558980014785906
17	18.311497860891272	24.524911829937825	26.987007792900343	30.176582516270557
18	21.988886330307476	25.57022700003636	27.672370956599725	24.76851571305644
19	24.059822326720074	28.17927306662142	25.155433820945085	22.60547078571342
20	27.29848141459927	27.207887433190724	23.36415750627189	22.129473645938116
21	21.447140381282495	29.631503678297438	24.19101695531505	24.730338985105014
22	21.61014894983699	25.97017367381319	23.806522766661413	28.613154609688408
23	20.665729417895797	29.281247348838335	21.913441844117752	28.139581389148116
24	19.254705433214966	28.61436656930591	29.06036770854795	23.070560288931173
25	21.707105719237436	26.843693568130313	26.007441432051483	25.441759280580772
26	22.320660275599614	30.58652785689181	23.553223206602755	23.53958866090582
27	20.566651719164717	28.06201597362776	26.00289658348584	25.36843572372168
28	21.78224721552278	27.23030868611458	29.558180121438355	21.42926397692429
29	20.769654955096897	26.900958660057444	28.743440268570254	23.585946116275405
30	24.73094496491377	26.768552071844965	26.035316503254112	22.46518645998715
31	28.2462338354886	28.3116796548339	21.640144950370257	21.801941559307245
32	29.911769339845595	24.495521809213315	22.865133133763983	22.727575717177103
33	25.944419531941193	28.338645756323398	22.47154924797906	23.24538546375635
34	22.980269297427007	26.557065118590252	25.851401631297644	24.611263952685096
35	26.673110251966403	25.54507883797312	25.396007805019938	22.385803105040537
36	28.083831246742857	26.06167662493486	26.162269273187817	19.692222855134467
37	25.006968767800657	29.162475306322794	23.988619699191624	21.841936226684926
38	25.60294990970901	28.79313061288798	24.25828071408661	21.34563876331641
39	23.316285101380423	27.170922664856807	26.647659099998787	22.865133133763983
40	26.538885724327667	26.02198494746155	23.637151410115013	23.80197791809577
41	22.969967640678213	21.835573438693025	28.677388469416197	26.517070451212565
42	28.499230405642884	23.514743488746955	22.98178424694889	25.00424185866127
43	24.061337276241957	24.466131788488806	25.879579692404647	25.592951242864586
44	24.42098629273673	24.473403546193843	25.30329289428076	25.80231726678867
45	24.03921901322248	27.952333628243508	24.75760807649889	23.250839282035123
46	22.943001539188714	26.029559695070958	28.43984438438511	22.587594381355213
47	22.660614948309924	26.46889505641672	25.16512949788513	25.705360497388224
48	24.36372120080959	21.46622874525821	31.247651828241086	22.922398225691122
49	23.969531335215912	22.676370423337495	29.030068718110314	24.324029523336282
50	21.710438608185576	24.802450582346598	27.054877531480653	26.432233277987176
51	21.260195610282263	26.111972949061336	28.1089794088061	24.5188520318503
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1584.0
1	1283.0
2	982.0
3	704.0
4	426.0
5	399.5
6	373.0
7	407.0
8	441.0
9	485.5
10	530.0
11	549.5
12	569.0
13	607.0
14	645.0
15	637.0
16	629.0
17	619.5
18	610.0
19	604.5
20	599.0
21	574.5
22	550.0
23	754.0
24	958.0
25	1152.5
26	1550.5
27	1754.0
28	2072.0
29	2390.0
30	2795.5
31	3201.0
32	3701.5
33	4202.0
34	4640.0
35	5078.0
36	6221.0
37	7364.0
38	8597.5
39	9831.0
40	11001.0
41	12171.0
42	16885.5
43	21600.0
44	24828.0
45	28056.0
46	30277.0
47	32498.0
48	32944.5
49	33391.0
50	31220.5
51	29050.0
52	26855.5
53	24661.0
54	21707.5
55	18754.0
56	18018.5
57	17283.0
58	15906.0
59	14529.0
60	13508.5
61	12488.0
62	11458.0
63	10428.0
64	9008.5
65	7589.0
66	7252.5
67	6916.0
68	6041.0
69	5166.0
70	4632.5
71	4099.0
72	3346.0
73	2593.0
74	2269.5
75	1583.0
76	1220.0
77	945.0
78	670.0
79	587.0
80	504.0
81	340.5
82	177.0
83	129.0
84	81.0
85	65.0
86	49.0
87	35.5
88	22.0
89	21.5
90	21.0
91	17.0
92	13.0
93	8.5
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	330044.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.756153883891795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.8488361796452	24.25086939106943
2	9.124466875990098	5.612670146928498
3	3.1486242104624336	2.905187122185898
4	1.424545361189613	1.7525414537332786
5	0.7897385441583612	1.2144660096087616
6	0.5048325079726985	0.9316023780479608
7	0.3728762722550979	0.8027768006390805
8	0.2979011497344451	0.7329834882736708
9	0.24391906216187065	0.6751810989958557
>10	4.032661871932805	31.73996830235569
>50	1.1376225112194769	23.317114838414245
>100	0.0669777752651186	3.318103105780301
>500	0.001999336575078167	0.4664160870523274
>1k	0.004998341437695417	2.2801197769150034
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGCT	2197	0.6656688199149204	RNA PCR Primer, Index 17 (96% over 25bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCC	1347	0.4081274011949922	No Hit
AATCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCT	1337	0.4050975021512283	TruSeq Adapter, Index 9 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1301	0.3941898655936784	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGT	1234	0.37388954200046054	No Hit
AAACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCT	840	0.2545115196761644	RNA PCR Primer, Index 47 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTT	677	0.20512416526281343	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	418	0.12664978002932942	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	393	0.11907503241991976	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCT	359	0.10877337567112265	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0298990437638615E-4	0.0	0.0	0.8801856722134018	0.0
2	3.0298990437638615E-4	0.0	0.0	1.1110639793482082	0.0
3	3.0298990437638615E-4	0.0	0.0	1.4289003890390373	0.0
4	3.0298990437638615E-4	0.0	0.0	3.2016943195452727	0.0
5	3.0298990437638615E-4	0.0	0.0	3.4862018397546994	0.0
6	3.0298990437638615E-4	0.0	0.0	4.899346753766165	0.0
7	3.0298990437638615E-4	0.0	0.0	6.0285901273769555	0.0
8	3.0298990437638615E-4	0.0	0.0	6.778187150804135	0.0
9	3.0298990437638615E-4	0.0	0.0	8.067712183830034	0.0
10	3.0298990437638615E-4	0.0	0.0	9.483886996885264	0.0
11	3.0298990437638615E-4	0.0	0.0	11.233653694658894	0.0
12	3.0298990437638615E-4	0.0	0.0	11.97022215219789	0.0
13	3.0298990437638615E-4	0.0	0.0	12.316236622995723	0.0
14	3.0298990437638615E-4	0.0	0.0	12.664069033219812	0.0
15	3.0298990437638615E-4	0.0	0.0	12.929185199549151	0.0
16	3.0298990437638615E-4	0.0	0.0	13.468507229339119	0.0
17	3.0298990437638615E-4	0.0	0.0	14.088727563597581	0.0
18	3.0298990437638615E-4	0.0	0.0	14.926191659293913	0.0
19	3.0298990437638615E-4	0.0	0.0	15.229484553574675	0.0
20	3.0298990437638615E-4	0.0	0.0	15.581558822460035	0.0
21	3.0298990437638615E-4	0.0	0.0	15.95332743512986	0.0
22	3.0298990437638615E-4	0.0	0.0	16.330246876174087	0.0
23	3.0298990437638615E-4	0.0	0.0	16.79442740967871	0.0
24	3.0298990437638615E-4	0.0	0.0	17.094387415011333	0.0
25	3.0298990437638615E-4	0.0	0.0	17.381924834264524	0.0
26	3.0298990437638615E-4	0.0	0.0	17.609773242355566	0.0
27	3.0298990437638615E-4	0.0	0.0	17.851559186047922	0.0
28	3.0298990437638615E-4	0.0	0.0	18.11000957448098	0.0
29	3.0298990437638615E-4	0.0	0.0	18.37058089224467	0.0
30	3.0298990437638615E-4	0.0	0.0	18.77234550544776	0.0
31	3.0298990437638615E-4	0.0	0.0	19.03109888378519	0.0
32	3.0298990437638615E-4	0.0	0.0	19.291064221740132	0.0
33	3.0298990437638615E-4	0.0	0.0	19.56617905491389	0.0
34	3.0298990437638615E-4	0.0	0.0	19.790694574056793	0.0
35	3.0298990437638615E-4	0.0	0.0	20.11277284240889	0.0
36	3.0298990437638615E-4	0.0	0.0	20.38122189768637	0.0
37	3.0298990437638615E-4	0.0	0.0	20.640278265928178	0.0
38	3.0298990437638615E-4	0.0	0.0	20.89357782598684	0.0
39	3.0298990437638615E-4	0.0	0.0	21.14505944661924	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGATC	20	7.025969E-4	45.000004	43
TTTAGAC	20	7.025969E-4	45.000004	34
GAGTGTA	20	7.025969E-4	45.000004	45
GGAATAC	20	7.025969E-4	45.000004	41
AGTCCGA	20	7.025969E-4	45.000004	11
GGTACAG	20	7.025969E-4	45.000004	1
TCCGCAT	25	3.8847415E-5	45.000004	31
CGTATCG	20	7.025969E-4	45.000004	1
GTCGAAA	20	7.025969E-4	45.000004	26
CTCAAAC	20	7.025969E-4	45.000004	22
CATGCGA	20	7.025969E-4	45.000004	36
CTATCGT	20	7.025969E-4	45.000004	19
ACGTTAA	20	7.025969E-4	45.000004	11
CGTGCCG	20	7.025969E-4	45.000004	13
GACACCC	20	7.025969E-4	45.000004	11
ACCGCGA	25	3.8847415E-5	45.000004	36
CGCATGA	25	3.8847415E-5	45.000004	33
CGCATCC	25	3.8847415E-5	45.000004	26
CCAATCG	20	7.025969E-4	45.000004	1
CACGCCC	30	2.160783E-6	45.000004	13
>>END_MODULE