SRR3552962_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552962_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	587057
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGCT	4816	0.8203632696654669	RNA PCR Primer, Index 40 (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2169	0.3694700855283218	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCC	1291	0.2199105027280145	No Hit
AATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCT	1262	0.21497060762413192	TruSeq Adapter, Index 19 (95% over 22bp)
AATGATACCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGT	1151	0.19606273326099508	No Hit
AAACTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCT	1044	0.17783622373977315	Illumina PCR Primer Index 8 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTT	728	0.1240084012285008	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCGCG	35	1.2105738E-7	45.000004	1
CGCGGCC	35	1.2105738E-7	45.000004	26
TTGTCGC	55	1.8189894E-12	45.000004	38
GAATACG	55	1.8189894E-12	45.000004	1
AACGGGT	35	1.2105738E-7	45.000004	4
TAGGGCG	35	1.2105738E-7	45.000004	5
GCTAGCG	35	1.2105738E-7	45.000004	1
CGGTAGC	35	1.2105738E-7	45.000004	16
CCACGCG	55	1.8189894E-12	45.000004	1
CTTCGCG	40	6.8048394E-9	45.0	1
CGGGTAG	40	6.8048394E-9	45.0	6
CCGGGTG	20	7.030464E-4	45.0	5
CCGGGTA	45	3.8380676E-10	45.0	5
ACCGGAT	20	7.030464E-4	45.0	4
ACGCCTA	20	7.030464E-4	45.0	29
TTAATCG	80	0.0	45.0	20
CGTGCCG	25	3.8884657E-5	45.0	15
CGTTGTG	20	7.030464E-4	45.0	33
ATAGCCG	25	3.8884657E-5	45.0	1
CTCCACG	50	2.1827873E-11	45.0	1