##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552962_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	587057
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.115295448312516	31.0	31.0	34.0	28.0	34.0
2	31.316420722348937	31.0	31.0	34.0	28.0	34.0
3	31.339752357948207	31.0	31.0	34.0	28.0	34.0
4	35.36859112488225	37.0	35.0	37.0	33.0	37.0
5	35.03096121841661	37.0	35.0	37.0	32.0	37.0
6	35.08678373650259	36.0	35.0	37.0	32.0	37.0
7	35.1812975571367	37.0	35.0	37.0	32.0	37.0
8	35.53163321449195	37.0	35.0	37.0	33.0	37.0
9	35.88574363307141	39.0	37.0	39.0	32.0	39.0
10	35.51713717748021	37.0	35.0	39.0	30.0	39.0
11	36.023304381005595	37.0	35.0	39.0	31.0	39.0
12	36.03548037073061	37.0	35.0	39.0	32.0	39.0
13	35.84243097348298	37.0	35.0	39.0	31.0	39.0
14	36.88589353333663	38.0	35.0	40.0	31.0	41.0
15	36.92215917704755	38.0	35.0	40.0	32.0	41.0
16	36.91114491437799	38.0	35.0	40.0	32.0	41.0
17	36.91384141573987	38.0	35.0	40.0	32.0	41.0
18	36.791849854443434	38.0	35.0	40.0	31.0	41.0
19	36.859441246761385	39.0	35.0	40.0	31.0	41.0
20	36.794938140589416	38.0	35.0	40.0	31.0	41.0
21	36.78959283340459	38.0	35.0	40.0	31.0	41.0
22	36.812999419136474	39.0	35.0	40.0	31.0	41.0
23	36.742880163255016	38.0	35.0	40.0	31.0	41.0
24	36.3698175815977	38.0	35.0	40.0	30.0	41.0
25	36.51401141626793	38.0	35.0	40.0	31.0	41.0
26	36.421451409318	38.0	35.0	40.0	31.0	41.0
27	36.30550696099357	38.0	35.0	40.0	30.0	41.0
28	36.23911136397317	38.0	35.0	40.0	30.0	41.0
29	36.03452986677614	38.0	35.0	40.0	30.0	41.0
30	35.95925267904139	38.0	35.0	40.0	30.0	41.0
31	35.93503867597184	38.0	35.0	40.0	30.0	41.0
32	35.82984786826492	38.0	34.0	40.0	29.0	41.0
33	35.80664228516141	38.0	34.0	40.0	29.0	41.0
34	35.81544722233105	38.0	35.0	40.0	29.0	41.0
35	35.71260882674085	38.0	34.0	40.0	29.0	41.0
36	35.5744569948063	38.0	34.0	40.0	28.0	41.0
37	35.43997090572125	38.0	34.0	40.0	27.0	41.0
38	35.37393302524286	38.0	34.0	40.0	27.0	41.0
39	35.27038771362917	38.0	34.0	40.0	27.0	41.0
40	35.14851198435586	38.0	34.0	40.0	26.0	41.0
41	35.242332516263325	38.0	34.0	40.0	27.0	41.0
42	35.186058593969584	38.0	34.0	40.0	27.0	41.0
43	35.095442180231224	38.0	34.0	40.0	26.0	41.0
44	35.01200053827822	38.0	34.0	40.0	26.0	41.0
45	34.7470364887907	37.0	33.0	40.0	25.0	41.0
46	34.57447402892734	37.0	33.0	40.0	24.0	41.0
47	34.46777229468348	37.0	33.0	40.0	24.0	41.0
48	34.36702228233374	37.0	33.0	40.0	24.0	41.0
49	34.26500833820225	37.0	33.0	40.0	24.0	41.0
50	34.13561885813473	37.0	33.0	40.0	23.0	41.0
51	32.22298686498926	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	6.0
11	3.0
12	8.0
13	6.0
14	22.0
15	24.0
16	90.0
17	153.0
18	393.0
19	695.0
20	1252.0
21	1836.0
22	2666.0
23	3437.0
24	4276.0
25	5082.0
26	5964.0
27	7524.0
28	9576.0
29	12090.0
30	15467.0
31	20125.0
32	26143.0
33	34945.0
34	53386.0
35	61501.0
36	63269.0
37	84021.0
38	103442.0
39	69648.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.37212740841179	31.921942843710237	21.57115918897143	26.134770558906546
2	20.685725576903096	32.771093777946604	21.946250534445547	24.596930110704754
3	21.00307125202493	33.16441163294195	20.618440798764006	25.214076316269118
4	18.96698276317291	33.947129495091616	19.170710850905447	27.915176890830022
5	17.8093438967596	36.3395717962651	18.218673825539938	27.632410481435365
6	20.46053449665024	37.253282049272904	19.606954690941425	22.679228763135438
7	83.57570048564278	6.111161267134197	5.459606137053131	4.853532110169882
8	86.19657035006823	4.764273315879038	4.341997455102316	4.697158878950425
9	76.47434576199585	12.112622794720105	7.6910759943242315	3.7219554489598115
10	34.680107723781504	42.03254539167406	11.025334848234499	12.262012036309933
11	25.67740440877121	25.30197238087613	32.586273564577205	16.434349645775452
12	26.82686689708154	25.216801775636778	30.438270900440674	17.518060426841007
13	22.168375472909783	32.934791681216645	26.682758233016557	18.214074612857015
14	16.77111421889186	34.70071901024943	30.324142289419935	18.204024481438772
15	15.891301866769325	30.402839928661102	34.029404299752834	19.67645390481674
16	17.654844418855408	29.74907036284381	32.89953105064755	19.696554167653225
17	17.805766731339546	27.786228594497636	29.241794238038217	25.166210436124604
18	19.940823463479695	27.859475315003486	29.652657237712866	22.547043983803956
19	20.743641588465856	31.291339682518053	28.664167193304905	19.300851535711182
20	24.095275245844952	29.755543328842005	27.33312097462427	18.816060450688774
21	19.37426859742751	32.496333405444446	26.998230154823126	21.131167842304922
22	19.15333604743662	29.25695460577082	27.63666901169733	23.95304033509523
23	19.626543930146475	31.020156475435947	24.58841305018082	24.76488654423676
24	16.253617621457543	31.772553602120407	30.043079292130066	21.930749484291987
25	17.69163812031881	31.166138892816196	28.168133588390905	22.974089398474085
26	20.293770451591584	33.5664168896717	24.598974205230498	21.54083845350622
27	16.845723669081536	33.22726753960859	26.767077132203514	23.159931659106356
28	19.14958854080609	31.215197161434066	29.82810868450593	19.80710561325391
29	18.62136044711161	29.50224594886016	28.591942520061934	23.284451083966292
30	19.55483028053494	32.38152342958179	26.043808352510915	22.01983793737235
31	23.728019596052853	31.29048797646566	22.94802719327084	22.03346523421065
32	21.697041343515195	32.276252561505956	22.91906918748946	23.107636907489393
33	22.072643712620753	30.003389790088526	23.97024479735358	23.953721699937145
34	18.424957031429656	30.298080084216693	26.497597337226196	24.77936554712745
35	18.372151256181258	30.143750947522985	25.644187872727862	25.839909923567898
36	22.086441350669524	29.436664582825863	27.25476401780406	21.222130048700553
37	19.810342096253002	32.36721476790158	24.265446115113185	23.55699702073223
38	21.819687015059866	30.198430476086653	25.569067398906746	22.412815109946735
39	20.40381087356083	28.79584094900495	27.348962707198787	23.45138547023543
40	21.976911952331715	28.52039921166088	24.30036606326132	25.20232277274609
41	18.79272370485319	24.637300977588204	29.825212883927794	26.744762433630804
42	22.489468654662154	26.903350100586486	25.283064506512996	25.324116738238367
43	20.289000897698177	27.093621232691206	26.95002359225765	25.667354277352967
44	21.45481614221447	28.132532275400855	25.860350868825343	24.55230071355933
45	22.09938728266591	30.11649635384639	25.110338519087584	22.673777844400117
46	19.355020040643414	28.449707609312213	29.264108936610928	22.93116341343345
47	20.077948137915058	27.754545129348596	26.05811701419113	26.109389718545216
48	20.96235970272052	25.050037730578122	31.054224717531685	22.933377849169673
49	21.603012995330946	23.646937179864988	29.325942796014697	25.424107028789365
50	19.762816898529444	26.525533295744708	26.326234079484617	27.385415726241234
51	19.165770955801566	26.383298384994987	29.490662746547606	24.96026791265584
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2588.0
1	2127.5
2	1667.0
3	1222.5
4	778.0
5	754.0
6	730.0
7	773.5
8	817.0
9	905.5
10	994.0
11	1111.5
12	1229.0
13	1325.5
14	1422.0
15	1557.0
16	1692.0
17	1676.0
18	1660.0
19	1773.5
20	1887.0
21	2009.0
22	2131.0
23	2319.0
24	2507.0
25	3228.0
26	4501.0
27	5053.0
28	6281.5
29	7510.0
30	8658.5
31	9807.0
32	12262.5
33	14718.0
34	15539.0
35	16360.0
36	18634.5
37	20909.0
38	23066.5
39	25224.0
40	28729.5
41	32235.0
42	38683.0
43	45131.0
44	50571.0
45	56011.0
46	59896.0
47	63781.0
48	66296.5
49	68812.0
50	63922.5
51	59033.0
52	51098.5
53	43164.0
54	36794.0
55	30424.0
56	25204.0
57	19984.0
58	17062.0
59	14140.0
60	12348.5
61	10557.0
62	8955.0
63	7353.0
64	6127.0
65	4901.0
66	3855.5
67	2810.0
68	2148.5
69	1487.0
70	1310.0
71	1133.0
72	940.5
73	748.0
74	768.0
75	638.0
76	488.0
77	342.5
78	197.0
79	162.5
80	128.0
81	96.0
82	64.0
83	46.0
84	28.0
85	20.0
86	12.0
87	9.0
88	6.0
89	5.0
90	4.0
91	3.5
92	3.0
93	2.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	587057.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.845419876371515
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.14671509172035	28.012018144218942
2	9.753740236014712	6.992538282500127
3	3.333252840099396	3.5844554282241203
4	1.639062862954658	2.350115861055092
5	0.9667220989661355	1.732627977060415
6	0.6508156088241612	1.399725525623905
7	0.47919608100549843	1.202388930872768
8	0.38253218823447976	1.0969621522793687
9	0.28388154196354415	0.9158267760151517
>10	3.6070693812167467	30.004304880760213
>50	0.6823569843063194	16.391699964940052
>100	0.07037549385172095	4.006905444383416
>500	0.0014265302807781274	0.31055733025636306
>1k	0.0028530605615562548	1.9998733018100485
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGCT	4816	0.8203632696654669	RNA PCR Primer, Index 40 (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2169	0.3694700855283218	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCC	1291	0.2199105027280145	No Hit
AATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCT	1262	0.21497060762413192	TruSeq Adapter, Index 19 (95% over 22bp)
AATGATACCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGT	1151	0.19606273326099508	No Hit
AAACTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCT	1044	0.17783622373977315	Illumina PCR Primer Index 8 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTT	728	0.1240084012285008	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.146226005311239	0.0
2	5.110236314361297E-4	0.0	0.0	1.4450044884908961	0.0
3	5.110236314361297E-4	0.0	0.0	1.8277611884365572	0.0
4	5.110236314361297E-4	0.0	0.0	3.8135990201973575	0.0
5	5.110236314361297E-4	0.0	0.0	4.107778290694089	0.0
6	5.110236314361297E-4	0.0	0.0	5.650217951578807	0.0
7	5.110236314361297E-4	0.0	0.0	6.852486215137542	0.0
8	5.110236314361297E-4	0.0	0.0	7.6966972542700285	0.0
9	5.110236314361297E-4	0.0	0.0	9.186842163537783	0.0
10	5.110236314361297E-4	0.0	0.0	11.065024350276039	0.0
11	5.110236314361297E-4	0.0	0.0	13.142165070853427	0.0
12	5.110236314361297E-4	0.0	0.0	14.131677162524252	0.0
13	5.110236314361297E-4	0.0	0.0	14.532490030780657	0.0
14	5.110236314361297E-4	0.0	0.0	14.927000274249348	0.0
15	5.110236314361297E-4	0.0	0.0	15.257462222578047	0.0
16	6.813648419148397E-4	0.0	0.0	15.93695331117762	0.0
17	6.813648419148397E-4	0.0	0.0	16.90449138669669	0.0
18	6.813648419148397E-4	0.0	0.0	17.968783269767673	0.0
19	6.813648419148397E-4	0.0	0.0	18.505698765196566	0.0
20	6.813648419148397E-4	0.0	0.0	19.037504024311097	0.0
21	6.813648419148397E-4	0.0	0.0	19.62978041314557	0.0
22	6.813648419148397E-4	0.0	0.0	20.319662315584345	0.0
23	6.813648419148397E-4	0.0	0.0	20.99642794481626	0.0
24	6.813648419148397E-4	0.0	0.0	21.462311155475533	0.0
25	6.813648419148397E-4	0.0	0.0	21.863635047363374	0.0
26	6.813648419148397E-4	0.0	0.0	22.279437942141904	0.0
27	6.813648419148397E-4	0.0	0.0	22.706313015601552	0.0
28	6.813648419148397E-4	0.0	0.0	23.11700567406572	0.0
29	6.813648419148397E-4	0.0	0.0	23.564321692782812	0.0
30	6.813648419148397E-4	0.0	0.0	24.149103068356226	0.0
31	6.813648419148397E-4	0.0	0.0	24.604765806386773	0.0
32	6.813648419148397E-4	0.0	0.0	24.99723195532972	0.0
33	6.813648419148397E-4	0.0	0.0	25.404858472005273	0.0
34	6.813648419148397E-4	0.0	0.0	25.824579214624816	0.0
35	6.813648419148397E-4	0.0	0.0	26.29795743854515	0.0
36	6.813648419148397E-4	0.0	0.0	26.7560049535224	0.0
37	6.813648419148397E-4	0.0	0.0	27.16789000045992	0.0
38	6.813648419148397E-4	0.0	0.0	27.56989525718968	0.0
39	6.813648419148397E-4	0.0	0.0	27.996429648228368	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCGCG	35	1.2105738E-7	45.000004	1
CGCGGCC	35	1.2105738E-7	45.000004	26
TTGTCGC	55	1.8189894E-12	45.000004	38
GAATACG	55	1.8189894E-12	45.000004	1
AACGGGT	35	1.2105738E-7	45.000004	4
TAGGGCG	35	1.2105738E-7	45.000004	5
GCTAGCG	35	1.2105738E-7	45.000004	1
CGGTAGC	35	1.2105738E-7	45.000004	16
CCACGCG	55	1.8189894E-12	45.000004	1
CTTCGCG	40	6.8048394E-9	45.0	1
CGGGTAG	40	6.8048394E-9	45.0	6
CCGGGTG	20	7.030464E-4	45.0	5
CCGGGTA	45	3.8380676E-10	45.0	5
ACCGGAT	20	7.030464E-4	45.0	4
ACGCCTA	20	7.030464E-4	45.0	29
TTAATCG	80	0.0	45.0	20
CGTGCCG	25	3.8884657E-5	45.0	15
CGTTGTG	20	7.030464E-4	45.0	33
ATAGCCG	25	3.8884657E-5	45.0	1
CTCCACG	50	2.1827873E-11	45.0	1
>>END_MODULE