SRR3552961_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552961_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	639259
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCT	5202	0.8137546753350364	Illumina PCR Primer Index 11 (96% over 25bp)
AATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCT	5032	0.7871613852914078	Illumina PCR Primer Index 11 (95% over 22bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCC	4886	0.7643224420774678	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGT	4337	0.6784417583483378	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2614	0.40891094220026625	No Hit
AAACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCT	1681	0.26296070919611614	Illumina PCR Primer Index 11 (95% over 22bp)
AAAAACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTT	1460	0.22838943213939888	No Hit
AATGACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTT	1177	0.1841194257726524	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCT	858	0.13421789916137278	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCT	792	0.12389344537972873	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	771	0.12060839190375106	No Hit
AACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTG	736	0.11513330277712164	Illumina PCR Primer Index 11 (95% over 23bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	707	0.1105968003579144	No Hit
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	640	0.10011591545836664	Illumina PCR Primer Index 11 (95% over 24bp)

Adapter graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTGCGT	20	7.030936E-4	45.0	41
AGCGTCG	20	7.030936E-4	45.0	1
AGCGTAA	20	7.030936E-4	45.0	25
TCGTTGC	25	3.8888567E-5	45.0	15
TTGGCGA	20	7.030936E-4	45.0	21
GTACCGT	25	3.8888567E-5	45.0	23
TCGCAAG	35	1.2107921E-7	45.0	1
GATCGCG	20	7.030936E-4	45.0	9
CGAAACG	20	7.030936E-4	45.0	1
CTATCAC	20	7.030936E-4	45.0	25
TCACACG	20	7.030936E-4	45.0	1
CGTTGAT	90	0.0	45.0	25
TAGCGAT	20	7.030936E-4	45.0	28
GGCCGAT	75	0.0	45.0	8
GCGCCTA	20	7.030936E-4	45.0	26
ATTGTCG	20	7.030936E-4	45.0	38
GATCACT	20	7.030936E-4	45.0	43
CTAAGCG	25	3.8888567E-5	45.0	1
CGCGACA	20	7.030936E-4	45.0	22
TAACGCG	35	1.2107921E-7	45.0	1