FastQCFastQC Report
Sat 18 Jun 2016
SRR3552958_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552958_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072442
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGCT56900.5305648230860037Illumina Single End Adapter 1 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20460.19077954798487937No Hit
AAACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT13890.1295174937199401No Hit
AATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT12820.11954026418211894No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCC11700.10909680896496034No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGT11280.10518051325852586No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACC207.0330745E-445.00000430
CGGCATA207.0330745E-445.00000430
TAAGTCG207.0330745E-445.00000415
TCGATAT207.0330745E-445.00000443
TTATCCG207.0330745E-445.0000041
CGTAGTA207.0330745E-445.0000046
TACGATA207.0330745E-445.00000423
ACCGTTG207.0330745E-445.00000427
ACGTAGT207.0330745E-445.0000045
CGATCAA207.0330745E-445.00000443
TTCGACA207.0330745E-445.00000436
TATCGCG406.8139343E-945.0000041
AAAGCGT207.0330745E-445.00000438
TGTCCGA207.0330745E-445.00000435
AGCCGTA253.8906303E-545.032
ATCTACG502.1827873E-1145.01
CTATACG253.8906303E-545.01
GCTATCG351.211829E-745.09
CGCGCCA253.8906303E-545.025
TAACGGT253.8906303E-545.028