FastQCFastQC Report
Sat 18 Jun 2016
SRR3552957_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552957_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1633092
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT66520.40732549054186784No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG21860.1338565126765669No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18600.11389437949607248No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA17180.10519921719045836No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAC302.1660653E-645.00000439
TATTACG302.1660653E-645.0000041
TATAGCG302.1660653E-645.0000041
CTATGCG406.8175723E-945.01
GTATTCG207.03416E-445.044
ATTACCG453.8562575E-1045.01
CGATTGT351.2123564E-745.034
ATATGCG351.2123564E-745.041
TCTAGCG1050.045.01
ACGATAG700.045.01
GTTCGAA207.03416E-445.09
TACGAAC207.03416E-445.011
TGTTACG207.03416E-445.01
CATACCG700.045.01
CGTAAAA253.8915292E-545.041
TATACGT207.03416E-445.042
TCGAATC207.03416E-445.011
TCGGTAA207.03416E-445.037
AAATGCG502.1827873E-1145.01
GTAACGG3000.044.252