##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552957_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1633092
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.574826157987424	31.0	31.0	34.0	30.0	34.0
2	31.766103195655848	31.0	31.0	34.0	30.0	34.0
3	31.95652541314268	33.0	31.0	34.0	30.0	34.0
4	35.772121840043305	37.0	35.0	37.0	35.0	37.0
5	35.413993210425375	37.0	35.0	37.0	33.0	37.0
6	35.43506856931514	37.0	35.0	37.0	33.0	37.0
7	35.46894418685537	37.0	35.0	37.0	33.0	37.0
8	35.76716988387672	37.0	35.0	37.0	35.0	37.0
9	36.60281049689791	39.0	37.0	39.0	33.0	39.0
10	36.22432294077737	38.0	35.0	39.0	31.0	39.0
11	36.49066188555207	38.0	35.0	39.0	32.0	39.0
12	36.2764975886233	38.0	35.0	39.0	32.0	39.0
13	36.12335496101873	38.0	35.0	39.0	32.0	39.0
14	37.25315781352184	39.0	36.0	40.0	32.0	41.0
15	37.20603370783765	39.0	35.0	40.0	32.0	41.0
16	37.23905144351941	39.0	35.0	40.0	32.0	41.0
17	37.180069463324784	39.0	35.0	40.0	32.0	41.0
18	37.05534164639837	39.0	35.0	40.0	32.0	41.0
19	37.153947236285525	39.0	35.0	40.0	32.0	41.0
20	37.09310008254281	39.0	35.0	40.0	32.0	41.0
21	37.12477435441482	39.0	35.0	40.0	32.0	41.0
22	37.09388693349793	39.0	35.0	40.0	32.0	41.0
23	37.05657488984087	39.0	35.0	40.0	32.0	41.0
24	36.722184053317264	39.0	35.0	40.0	31.0	41.0
25	36.80679777991687	39.0	35.0	40.0	31.0	41.0
26	36.71943221814815	39.0	35.0	40.0	31.0	41.0
27	36.62342231790983	39.0	35.0	40.0	31.0	41.0
28	36.581236084678636	38.0	35.0	40.0	31.0	41.0
29	36.35878444080309	38.0	35.0	40.0	30.0	41.0
30	36.32244907206697	38.0	35.0	40.0	30.0	41.0
31	36.24865224984263	38.0	35.0	40.0	30.0	41.0
32	36.20215823725791	38.0	35.0	40.0	30.0	41.0
33	36.20450286940356	38.0	35.0	40.0	30.0	41.0
34	36.08483661667561	38.0	35.0	40.0	30.0	41.0
35	36.01152721340868	38.0	35.0	40.0	30.0	41.0
36	35.8764515410032	38.0	35.0	40.0	29.0	41.0
37	35.75954998248721	38.0	34.0	40.0	29.0	41.0
38	35.69960051240224	38.0	34.0	40.0	29.0	41.0
39	35.66308756640777	38.0	34.0	40.0	29.0	41.0
40	35.533255321806735	38.0	34.0	40.0	28.0	41.0
41	35.56044729874373	38.0	34.0	40.0	29.0	41.0
42	35.520989631937454	38.0	34.0	40.0	29.0	41.0
43	35.43508510237023	38.0	34.0	40.0	28.0	41.0
44	35.281718972354284	37.0	34.0	40.0	28.0	41.0
45	35.10105799305857	37.0	34.0	40.0	27.0	41.0
46	35.02653310407497	37.0	34.0	40.0	27.0	41.0
47	34.92613643321993	37.0	34.0	40.0	27.0	41.0
48	34.89960761549258	37.0	34.0	40.0	27.0	41.0
49	34.71828286465184	37.0	34.0	40.0	26.0	41.0
50	34.55213178436977	36.0	33.0	40.0	26.0	41.0
51	32.69861159077382	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	14.0
10	19.0
11	25.0
12	27.0
13	38.0
14	83.0
15	167.0
16	310.0
17	607.0
18	1003.0
19	1737.0
20	2818.0
21	4082.0
22	5820.0
23	7577.0
24	9754.0
25	11898.0
26	14563.0
27	18205.0
28	23110.0
29	29326.0
30	37568.0
31	48634.0
32	63889.0
33	89625.0
34	144101.0
35	156112.0
36	166894.0
37	228996.0
38	312840.0
39	253238.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.026060993501897	30.832188266184634	21.502891447634305	27.638859292679165
2	23.654944118273804	30.20736125092769	21.14963517058439	24.98805946021412
3	20.48047507427628	30.72435600688755	21.170148405601154	27.625020513235015
4	20.64782633189067	31.835928410646797	19.975482091639662	27.540763165822867
5	18.86966564039258	34.21503503783008	17.688348237576328	29.226951084201012
6	21.081788411185652	37.28761147565477	18.53582039468689	23.094779718472687
7	86.15736284299966	5.465950479213663	4.427429685529045	3.9492569922576313
8	88.55741133996126	4.2312374318164565	3.6590100251547373	3.552341203067555
9	80.48658618130516	9.5515133256424	6.108596453843384	3.8533040392090587
10	42.494115457059365	32.882532031263395	11.280748420787072	13.342604090890164
11	33.56167319416175	25.615335816965608	22.757872795898823	18.06511819297382
12	33.40185366164307	23.525925055048948	24.15411991486089	18.918101368447093
13	26.365752817355055	27.524536278421547	23.67319171240812	22.43651919181528
14	20.73049160733137	29.112873004092847	27.316770886147257	22.839864502428522
15	20.4301411065635	28.301712334638836	29.473293605014295	21.794852953783376
16	23.349878635128945	26.23703992181702	28.598082655478073	21.81499878757596
17	22.65242864455891	26.769098127968295	26.66267424003057	23.915798987442226
18	24.10623528864265	27.355776649447794	25.41546955101121	23.122518510898345
19	25.16906579666057	29.175208745128874	23.774165815520497	21.881559642690064
20	28.807929988022718	27.501083833611332	23.92222850886539	19.768757669500555
21	26.606400619193533	28.020895332289914	24.061044938068402	21.311659110448154
22	24.969077063631442	25.589678964810307	25.17512791685955	24.266116054698696
23	25.10256617508383	26.766342618786936	23.968031194813275	24.163060011315956
24	23.379087032451327	26.543881177545416	27.341386768167382	22.73564502183588
25	23.14462381788656	27.639900262814344	24.35331261190429	24.86216330739481
26	24.54068723623654	28.947664920286183	23.420909538470582	23.0907383050067
27	22.921060172972496	27.319893796552797	25.720657501230793	24.03838852924391
28	22.448949599900068	28.415974115359088	27.689683128690852	21.445393156049995
29	23.061836075371136	27.417071420348638	25.65268827475733	23.868404229522895
30	23.73362921378587	27.695745248889835	25.901480137065153	22.66914540025914
31	25.8344906471895	27.247148354164985	23.397518327197734	23.52084267144778
32	26.586560953087762	26.752320138730703	23.887754027329752	22.773364880851783
33	25.472233040147156	27.20967342929853	24.654397915120523	22.66369561543379
34	21.989453135524514	26.89830089180524	26.15400724515214	24.958238727518108
35	22.93710335976173	27.125967183722654	26.280148332120913	23.65678112439471
36	24.880533368603853	28.101846068684434	25.229503298038324	21.788117264673392
37	23.68623445586654	29.48523414480017	24.753902413336174	22.074628985997112
38	23.12790706218633	28.574691444205225	25.78568751791081	22.511713975697635
39	23.169117232831955	27.693234673857937	25.055844986075492	24.08180310723462
40	24.06967886683665	26.983782909964653	24.386317488543206	24.560220734655488
41	20.70899863571679	25.26361037834978	27.309116693976826	26.718274291956607
42	23.96845982957482	25.357603858202722	24.47841272873788	26.19552358348458
43	23.013094179629807	25.350255833719103	26.591337169002117	25.045312817648973
44	23.363533713961	24.714529248811456	25.954140979197742	25.967796058029798
45	23.552929045026243	26.63824205862254	25.5514692374955	24.25735965885572
46	21.830674573141014	26.8106756998381	27.400109730498954	23.958539996521935
47	22.15123214123883	26.66004119792394	25.84073646800058	25.347990192836654
48	22.966740391845654	24.27217817489768	29.291368765507393	23.469712667749278
49	22.109470868756933	25.543080242876705	28.15224126993458	24.195207618431784
50	21.122631180607094	25.769705564658942	26.617728823605773	26.48993443112819
51	21.402590913432924	25.707431057160285	27.541497968271226	25.348480061135565
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2466.0
1	2089.0
2	1712.0
3	1298.0
4	884.0
5	898.0
6	912.0
7	914.5
8	917.0
9	1063.0
10	1209.0
11	1259.0
12	1309.0
13	1518.0
14	1727.0
15	1929.5
16	2132.0
17	2431.0
18	2730.0
19	2996.5
20	3263.0
21	3553.5
22	3844.0
23	5310.0
24	6776.0
25	7154.0
26	10279.5
27	13027.0
28	15604.5
29	18182.0
30	21439.0
31	24696.0
32	30468.5
33	36241.0
34	39000.0
35	41759.0
36	43793.5
37	45828.0
38	50924.0
39	56020.0
40	65095.5
41	74171.0
42	85620.5
43	97070.0
44	109009.0
45	120948.0
46	128130.0
47	135312.0
48	128646.5
49	121981.0
50	118551.5
51	115122.0
52	109679.5
53	104237.0
54	99277.5
55	94318.0
56	91940.5
57	89563.0
58	89098.0
59	88633.0
60	83676.5
61	78720.0
62	74731.5
63	70743.0
64	60778.5
65	50814.0
66	43746.0
67	36678.0
68	31863.0
69	27048.0
70	22447.0
71	17846.0
72	16102.0
73	14358.0
74	11513.5
75	7049.5
76	5430.0
77	4486.5
78	3543.0
79	2686.5
80	1830.0
81	1513.5
82	1197.0
83	1029.0
84	861.0
85	662.5
86	464.0
87	317.5
88	171.0
89	139.5
90	108.0
91	87.0
92	66.0
93	40.0
94	14.0
95	11.0
96	8.0
97	5.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1633092.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.91151017552878
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.266314989433	17.473764519304773
2	10.612577749975905	4.8630036621432655
3	3.9495733004753273	2.7147206658851175
4	1.8240163311169437	1.671638749228661
5	0.9329095117382643	1.0687182885519413
6	0.5623900828093381	0.7731123662941575
7	0.3672777075762003	0.5890420854063898
8	0.2638547457195525	0.4836248553132068
9	0.20629142992470983	0.4253803376259934
>10	2.614357370591933	15.275525977959889
>50	1.4808984699796266	25.11140060255297
>100	0.9134539087203918	27.754680569920826
>500	0.0031744705767233986	0.4842733335124282
>1k	0.002645392147269499	0.9079367379244273
>5k	2.645392147269499E-4	0.40317724837605406
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT	6652	0.40732549054186784	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	2186	0.1338565126765669	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1860	0.11389437949607248	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1718	0.10519921719045836	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.123353736347983E-5	0.0	0.0	0.5452234166844244	0.0
2	6.123353736347983E-5	0.0	0.0	0.6815292708555305	0.0
3	6.123353736347983E-5	0.0	0.0	0.872761608041678	0.0
4	6.123353736347983E-5	0.0	0.0	1.8076140229699245	0.0
5	6.123353736347983E-5	0.0	0.0	1.9170995877758266	0.0
6	6.123353736347983E-5	0.0	0.0	2.6165702850788564	0.0
7	6.123353736347983E-5	0.0	0.0	3.129646094647454	0.0
8	6.123353736347983E-5	0.0	0.0	3.378499190492636	0.0
9	6.123353736347983E-5	0.0	0.0	3.999958361194593	0.0
10	6.123353736347983E-5	0.0	0.0	4.774440141767886	0.0
11	6.123353736347983E-5	0.0	0.0	5.743767038231772	0.0
12	6.123353736347983E-5	0.0	0.0	6.164747607605695	0.0
13	6.123353736347983E-5	0.0	0.0	6.323709870601289	0.0
14	6.123353736347983E-5	0.0	0.0	6.5305567598151235	0.0
15	6.123353736347983E-5	0.0	0.0	6.706541946197765	0.0
16	1.2246707472695966E-4	0.0	0.0	7.06139029521913	0.0
17	1.2246707472695966E-4	0.0	0.0	7.484820206087593	0.0
18	1.2246707472695966E-4	0.0	0.0	7.930967759317907	0.0
19	1.2246707472695966E-4	0.0	0.0	8.204314270108481	0.0
20	1.2246707472695966E-4	0.0	0.0	8.457270012957016	0.0
21	1.2246707472695966E-4	0.0	0.0	8.789829354378076	0.0
22	1.2246707472695966E-4	0.0	0.0	9.142595763129084	0.0
23	1.2246707472695966E-4	0.0	0.0	9.498852483509808	0.0
24	1.2246707472695966E-4	0.0	0.0	9.762769029546407	0.0
25	1.2246707472695966E-4	0.0	0.0	10.0037229990717	0.0
26	1.2246707472695966E-4	0.0	0.0	10.21277429563062	0.0
27	1.2246707472695966E-4	0.0	0.0	10.424948502595077	0.0
28	1.2246707472695966E-4	0.0	0.0	10.64477690172997	0.0
29	1.2246707472695966E-4	0.0	0.0	10.884383733433266	0.0
30	1.2246707472695966E-4	0.0	0.0	11.158648747284293	0.0
31	1.837006120904395E-4	0.0	0.0	11.393050728311694	0.0
32	1.837006120904395E-4	0.0	0.0	11.62457473308301	0.0
33	1.837006120904395E-4	0.0	0.0	11.87250932586774	0.0
34	1.837006120904395E-4	0.0	0.0	12.100849186696157	0.0
35	2.4493414945391933E-4	0.0	0.0	12.365867936405298	0.0
36	2.4493414945391933E-4	0.0	0.0	12.61386376272739	0.0
37	2.4493414945391933E-4	0.0	0.0	12.862533157960483	0.0
38	2.4493414945391933E-4	0.0	0.0	13.130184949776252	0.0
39	2.4493414945391933E-4	0.0	0.0	13.401082119072287	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCGAC	30	2.1660653E-6	45.000004	39
TATTACG	30	2.1660653E-6	45.000004	1
TATAGCG	30	2.1660653E-6	45.000004	1
CTATGCG	40	6.8175723E-9	45.0	1
GTATTCG	20	7.03416E-4	45.0	44
ATTACCG	45	3.8562575E-10	45.0	1
CGATTGT	35	1.2123564E-7	45.0	34
ATATGCG	35	1.2123564E-7	45.0	41
TCTAGCG	105	0.0	45.0	1
ACGATAG	70	0.0	45.0	1
GTTCGAA	20	7.03416E-4	45.0	9
TACGAAC	20	7.03416E-4	45.0	11
TGTTACG	20	7.03416E-4	45.0	1
CATACCG	70	0.0	45.0	1
CGTAAAA	25	3.8915292E-5	45.0	41
TATACGT	20	7.03416E-4	45.0	42
TCGAATC	20	7.03416E-4	45.0	11
TCGGTAA	20	7.03416E-4	45.0	37
AAATGCG	50	2.1827873E-11	45.0	1
GTAACGG	300	0.0	44.25	2
>>END_MODULE