FastQCFastQC Report
Sat 18 Jun 2016
SRR3552955_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552955_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704421
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT49240.6990137999860878No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24030.3411312269225364No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCC17790.2525478371598802No Hit
AATCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT16450.2335251220505919No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGT15430.21904514487785004No Hit
AAACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT14190.20144203537373248No Hit
AAAAACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT9730.13812762538311607No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGC253.8892613E-545.00000415
TCTTGGC351.2110286E-745.00000417
TTAATCG950.045.00000420
ATAATCG253.8892613E-545.0000041
CACATCG253.8892613E-545.00000419
TAGCGAT253.8892613E-545.00000436
TCCTCGA351.2110286E-745.00000438
ATTCGAC253.8892613E-545.00000445
GTCTAGT253.8892613E-545.00000445
TTCAACG253.8892613E-545.0000041
ATATGCG351.2110286E-745.0000041
CACTGCG502.1827873E-1145.0000041
ATGTCGT253.8892613E-545.00000438
TTATCGT302.164299E-645.00000417
CCTAAAC253.8892613E-545.00000434
TCGATAG253.8892613E-545.0000041
CCATGCG253.8892613E-545.0000041
TCGGACA302.164299E-645.00000443
CCGTAAG253.8892613E-545.00000422
GTTCGAC351.2110286E-745.00000438