##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3552955_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 704421 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.613635879679908 31.0 31.0 34.0 30.0 34.0 2 31.785895082628144 31.0 31.0 34.0 30.0 34.0 3 31.98894978996935 33.0 31.0 34.0 30.0 34.0 4 35.79844297657225 37.0 35.0 37.0 35.0 37.0 5 35.4260378381678 37.0 35.0 37.0 33.0 37.0 6 35.44415484490099 37.0 35.0 37.0 33.0 37.0 7 35.443401034324644 37.0 35.0 37.0 33.0 37.0 8 35.741380509666804 37.0 35.0 37.0 35.0 37.0 9 36.63879554982035 39.0 37.0 39.0 33.0 39.0 10 36.329615386253394 39.0 35.0 39.0 32.0 39.0 11 36.47744317673664 38.0 35.0 39.0 32.0 39.0 12 36.13556523726578 37.0 35.0 39.0 32.0 39.0 13 35.88905214353348 37.0 35.0 39.0 31.0 39.0 14 36.97578294798139 39.0 35.0 40.0 32.0 41.0 15 37.00765025460627 39.0 35.0 40.0 32.0 41.0 16 37.05375336623979 39.0 35.0 40.0 32.0 41.0 17 37.01020980351239 38.0 35.0 40.0 32.0 41.0 18 36.857115276233955 38.0 35.0 40.0 32.0 41.0 19 36.93266668654115 39.0 35.0 40.0 32.0 41.0 20 36.86987327180763 38.0 35.0 40.0 31.0 41.0 21 36.8954545648128 39.0 35.0 40.0 32.0 41.0 22 36.8365636458879 38.0 35.0 40.0 31.0 41.0 23 36.79351836472791 38.0 35.0 40.0 31.0 41.0 24 36.441013257696746 38.0 35.0 40.0 31.0 41.0 25 36.555410755783825 38.0 35.0 40.0 31.0 41.0 26 36.421733593972924 38.0 35.0 40.0 31.0 41.0 27 36.38609581486072 38.0 35.0 40.0 31.0 41.0 28 36.384608068186495 38.0 35.0 40.0 31.0 41.0 29 36.19888390607321 38.0 35.0 40.0 30.0 41.0 30 36.21177392496816 38.0 35.0 40.0 30.0 41.0 31 36.04800112432764 38.0 35.0 40.0 30.0 41.0 32 36.01924985200611 38.0 35.0 40.0 30.0 41.0 33 36.01497258031774 38.0 35.0 40.0 30.0 41.0 34 35.907183346322725 38.0 35.0 40.0 30.0 41.0 35 35.829865946642705 38.0 35.0 40.0 30.0 41.0 36 35.6430912763816 38.0 34.0 40.0 29.0 41.0 37 35.53967442765051 38.0 34.0 40.0 29.0 41.0 38 35.48338280658867 38.0 34.0 40.0 29.0 41.0 39 35.476294715802055 38.0 34.0 40.0 29.0 41.0 40 35.367195185833474 38.0 34.0 40.0 28.0 41.0 41 35.40763691031358 38.0 34.0 40.0 29.0 41.0 42 35.32484267220881 37.0 34.0 40.0 29.0 41.0 43 35.23911268971254 37.0 34.0 40.0 28.0 41.0 44 35.08483137214819 37.0 34.0 40.0 28.0 41.0 45 34.93847713228311 37.0 34.0 40.0 27.0 41.0 46 34.906809990048565 37.0 34.0 40.0 27.0 41.0 47 34.79984128809334 37.0 34.0 40.0 27.0 41.0 48 34.73696553623472 37.0 34.0 40.0 27.0 41.0 49 34.59363931512547 36.0 34.0 40.0 26.0 41.0 50 34.40338377192049 36.0 33.0 40.0 26.0 41.0 51 32.50151826819473 35.0 31.0 38.0 20.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 5.0 9 11.0 10 11.0 11 19.0 12 13.0 13 19.0 14 29.0 15 38.0 16 104.0 17 260.0 18 505.0 19 879.0 20 1384.0 21 2243.0 22 2886.0 23 3946.0 24 4672.0 25 5568.0 26 6452.0 27 7688.0 28 9646.0 29 12456.0 30 15987.0 31 21112.0 32 28280.0 33 40732.0 34 69684.0 35 75142.0 36 68788.0 37 95689.0 38 129751.0 39 100421.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.033048418488374 31.859640754605557 21.131255314648485 26.976055512257584 2 21.8329663652844 31.92778182365375 22.296041713691103 23.94321009737075 3 20.810282487319373 32.302841624539866 21.402683906357137 25.48419198178362 4 19.539735470691532 33.094555670543606 20.42301407822879 26.94269478053607 5 18.216663046672373 35.29778356976865 17.97490421211179 28.510649171447188 6 21.43405719023141 37.310784317900804 19.478976350790223 21.776182141077566 7 84.12497639905682 6.683929070825544 4.8725123186276385 4.318582211490004 8 86.38584028585178 5.4566800251554115 3.951756123113877 4.205723565878928 9 79.30456360613894 9.405313015937912 7.1677306610677425 4.122392716855403 10 46.78849721970242 25.957062608866003 11.563965299160587 15.690474872270984 11 35.799188269514964 26.340498082822627 21.641461569146863 16.218852078515546 12 34.26232324135709 25.048940903238265 23.28451309657151 17.40422275883314 13 24.146497620031205 32.91000694187141 22.234856712108243 20.708638725989147 14 19.199030125450548 33.258804039061864 26.959020244995536 20.583145590492048 15 19.192925821348314 28.51150093481029 32.0204820696714 20.275091174169994 16 20.98134496274245 26.50034567396486 31.41303283121883 21.105276532073862 17 20.30433504963651 26.81791144784156 26.78242130771229 26.09533219480964 18 23.089885168102597 27.932443808461134 25.40029329051803 23.57737773291824 19 24.15004663404413 29.774949923412276 25.080882029354605 20.99412141318899 20 27.08437141993211 29.79439852020312 23.808631486000557 19.31259857386421 21 24.13073999781381 29.534752655017382 24.647334477535452 21.687172869633358 22 23.48425160521904 27.913988935593913 25.357847082923424 23.243912376263626 23 23.884580385877197 28.65573286429564 22.911440743532633 24.54824600629453 24 21.403109788038687 29.903140309559202 27.550144018988643 21.143605883413468 25 21.53697859660629 28.155747770154495 26.326585947891957 23.980687685347256 26 22.298455043219892 33.09753684231447 22.737964938580763 21.866043175884876 27 21.353139670736677 29.65882618490931 26.52462092981328 22.463413214540736 28 21.139347066597956 29.085731402101867 28.849792950522485 20.925128580777688 29 20.18139720422872 29.350487847466216 27.496340966552673 22.97177398175239 30 22.20433519159707 28.38572387819216 27.796729512606806 21.61321141760396 31 26.996923714653594 28.966910412949076 21.499359048069266 22.536806824328064 32 25.967709650904787 27.82185653181833 23.842985941645694 22.367447875631193 33 24.560170693377966 27.403356799413984 26.2845656219789 21.751906885229147 34 21.381247861719057 28.10648745565507 25.66036503738531 24.85189964524056 35 22.05669620865931 27.940677520971125 27.530695422197805 22.471930848171763 36 25.23945197545218 28.173634800779645 26.517664862347946 20.06924836142023 37 22.78140487009899 29.35517254596328 25.98346727312218 21.87995531081555 38 23.63842077394058 28.811321638622356 25.85087610959923 21.699381477837825 39 22.602392603286955 26.89527995332337 26.490124513607626 24.01220292978205 40 23.489930027639723 26.25844487884376 24.935372454824602 25.316252638691918 41 20.364242406174718 25.0791785026284 28.132892119911247 26.423686971285637 42 23.45585949311562 26.74934449711181 25.108564338655437 24.68623167111713 43 22.25061433432564 25.838383580273728 27.54602716273365 24.364974922666985 44 23.127362756079105 25.46630495115847 25.662352485232553 25.743979807529872 45 23.39864938722724 26.502759003493647 25.28998993499626 24.80860167428285 46 20.33386284622406 26.895421913883883 29.174456752425044 23.59625848746701 47 22.491663366083632 26.054021671699168 26.29932952027268 25.154985441944515 48 22.780411146175368 23.445070490516326 30.763989148534755 23.010529214773552 49 21.860364753464193 23.63898861618265 29.525809139704805 24.974837490648348 50 21.90039763153001 25.730635514841264 25.971258664917713 26.39770818871101 51 20.451406190332204 25.941021065527575 28.25512016251645 25.352452581623773 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2865.0 1 2396.0 2 1927.0 3 1398.0 4 869.0 5 851.5 6 834.0 7 830.5 8 827.0 9 903.0 10 979.0 11 1038.5 12 1098.0 13 1243.0 14 1388.0 15 1433.0 16 1478.0 17 1750.0 18 2022.0 19 1987.0 20 1952.0 21 2196.5 22 2441.0 23 2564.0 24 2687.0 25 2600.0 26 3604.5 27 4696.0 28 6067.0 29 7438.0 30 8648.5 31 9859.0 32 11686.0 33 13513.0 34 14133.5 35 14754.0 36 15696.5 37 16639.0 38 20013.0 39 23387.0 40 29319.0 41 35251.0 42 43818.5 43 52386.0 44 58456.5 45 64527.0 46 67374.5 47 70222.0 48 65624.5 49 61027.0 50 56762.5 51 52498.0 52 48273.5 53 44049.0 54 40934.5 55 37820.0 56 34836.0 57 31852.0 58 30828.5 59 29805.0 60 29781.0 61 29757.0 62 26717.5 63 23678.0 64 20964.5 65 18251.0 66 15032.0 67 11813.0 68 10406.0 69 8999.0 70 7506.5 71 6014.0 72 5199.0 73 4384.0 74 3709.0 75 2636.5 76 2239.0 77 1628.5 78 1018.0 79 885.5 80 753.0 81 571.0 82 389.0 83 345.0 84 301.0 85 196.5 86 92.0 87 70.5 88 49.0 89 37.5 90 26.0 91 17.0 92 8.0 93 8.0 94 8.0 95 5.5 96 3.0 97 2.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 704421.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.989521796053697 #Duplication Level Percentage of deduplicated Percentage of total 1 77.79154413586554 19.43973487731885 2 9.878731473843901 4.937295509659677 3 3.4295361166822596 2.57107402614554 4 1.609578457808767 1.6089038381548273 5 0.8697568101738596 1.08674033825529 6 0.5247601243157881 0.7868102738573541 7 0.3667921697777714 0.6416172644898412 8 0.2951102351024676 0.5899730921865315 9 0.2229182790957798 0.5013559074782493 >10 2.8512572225802253 18.11603939431995 >50 1.5772974723973636 28.951749011576116 >100 0.5759285605812468 18.262233508581403 >500 0.0033944708875122596 0.5677700603098371 >1k 0.0033944708875122596 1.9387028976665333 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT 4924 0.6990137999860878 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2403 0.3411312269225364 No Hit AATGATACGGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCC 1779 0.2525478371598802 No Hit AATCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT 1645 0.2335251220505919 No Hit AATGATACCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGT 1543 0.21904514487785004 No Hit AAACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT 1419 0.20144203537373248 No Hit AAAAACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT 973 0.13812762538311607 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.9064181789015375 0.0 2 0.0 0.0 0.0 1.1379558531048903 0.0 3 0.0 0.0 0.0 1.4269875543176596 0.0 4 0.0 0.0 0.0 2.9581741600548535 0.0 5 0.0 0.0 0.0 3.1465558238610147 0.0 6 0.0 0.0 0.0 4.224888241548733 0.0 7 0.0 0.0 0.0 5.038464213872102 0.0 8 0.0 0.0 0.0 5.427010268007342 0.0 9 0.0 0.0 0.0 6.375732693942969 0.0 10 0.0 0.0 0.0 7.394867557895066 0.0 11 0.0 0.0 0.0 8.925344360829675 0.0 12 0.0 0.0 0.0 9.502697960452627 0.0 13 0.0 0.0 0.0 9.731680344566673 0.0 14 0.0 0.0 0.0 10.006515989727735 0.0 15 0.0 0.0 0.0 10.235214452720745 0.0 16 0.0 0.0 0.0 10.693321181509353 0.0 17 0.0 0.0 0.0 11.213464675243923 0.0 18 0.0 0.0 0.0 11.827728020601317 0.0 19 0.0 0.0 0.0 12.160199653332311 0.0 20 0.0 0.0 0.0 12.44724390669784 0.0 21 0.0 0.0 0.0 12.821593904781373 0.0 22 0.0 0.0 0.0 13.192962731094047 0.0 23 0.0 0.0 0.0 13.575404481127054 0.0 24 0.0 0.0 0.0 13.84953032348553 0.0 25 0.0 0.0 0.0 14.095547974861624 0.0 26 0.0 0.0 0.0 14.32495624065722 0.0 27 0.0 0.0 0.0 14.551667255802993 0.0 28 0.0 0.0 0.0 14.788883352427028 0.0 29 0.0 0.0 0.0 15.049239020415348 0.0 30 0.0 0.0 0.0 15.331740535844332 0.0 31 0.0 0.0 0.0 15.585707978609383 0.0 32 0.0 0.0 0.0 15.817813495054803 0.0 33 0.0 0.0 0.0 16.03898804834041 0.0 34 0.0 0.0 0.0 16.2891225559715 0.0 35 0.0 0.0 0.0 16.577444454381684 0.0 36 0.0 0.0 0.0 16.819203288942266 0.0 37 0.0 0.0 0.0 17.060962123502847 0.0 38 1.4196056051707715E-4 0.0 0.0 17.317768777478243 0.0 39 1.4196056051707715E-4 0.0 0.0 17.598282845059984 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACCGGC 25 3.8892613E-5 45.000004 15 TCTTGGC 35 1.2110286E-7 45.000004 17 TTAATCG 95 0.0 45.000004 20 ATAATCG 25 3.8892613E-5 45.000004 1 CACATCG 25 3.8892613E-5 45.000004 19 TAGCGAT 25 3.8892613E-5 45.000004 36 TCCTCGA 35 1.2110286E-7 45.000004 38 ATTCGAC 25 3.8892613E-5 45.000004 45 GTCTAGT 25 3.8892613E-5 45.000004 45 TTCAACG 25 3.8892613E-5 45.000004 1 ATATGCG 35 1.2110286E-7 45.000004 1 CACTGCG 50 2.1827873E-11 45.000004 1 ATGTCGT 25 3.8892613E-5 45.000004 38 TTATCGT 30 2.164299E-6 45.000004 17 CCTAAAC 25 3.8892613E-5 45.000004 34 TCGATAG 25 3.8892613E-5 45.000004 1 CCATGCG 25 3.8892613E-5 45.000004 1 TCGGACA 30 2.164299E-6 45.000004 43 CCGTAAG 25 3.8892613E-5 45.000004 22 GTTCGAC 35 1.2110286E-7 45.000004 38 >>END_MODULE