FastQCFastQC Report
Sat 18 Jun 2016
SRR3552953_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552953_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664272
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCC84601.2735746802514631No Hit
AATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCT78671.1843040200399837No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGT72531.0918720042392274No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT64270.9675253510610111No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23510.35392128525664185No Hit
AATGACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTT16660.2508008767492834No Hit
AAACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCT15150.22806922465496063No Hit
AATGATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCT13330.20067080954789604No Hit
AACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG11690.17598212780306863No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG11310.1702615795939013No Hit
AATGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC9800.1475299274995785No Hit
AAAAACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTT8880.13368017920369968No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG207.0311327E-445.0000041
AGCGTTC207.0311327E-445.00000436
ACTATCG207.0311327E-445.00000412
GTCGACT207.0311327E-445.00000411
TACCAGT207.0311327E-445.00000443
ACGCATT207.0311327E-445.00000423
ACGCATC207.0311327E-445.00000411
CGACGAA207.0311327E-445.00000418
GCGCGTT207.0311327E-445.00000441
CGGTTGC207.0311327E-445.00000438
TAGAACG207.0311327E-445.0000041
GAATCTA207.0311327E-445.0000049
CCAATAC207.0311327E-445.00000431
CACGCAT207.0311327E-445.00000432
CAACCCA207.0311327E-445.00000424
ATGCTAT207.0311327E-445.00000410
CGTCTAA207.0311327E-445.0000041
CACGATT207.0311327E-445.00000416
TTCCGAA207.0311327E-445.00000433
TAACGCG207.0311327E-445.0000041